oru.sePublikasjoner
Endre søk
Link to record
Permanent link

Direct link
BETA
Ganda Mall, John Peter
Alternativa namn
Publikasjoner (10 av 10) Visa alla publikasjoner
Ganda Mall, J.-P., Östlund-Lagerström, L., Lindqvist, C. M., Algilani, S., Rasoal, D., Repsilber, D., . . . Schoultz, I. (2018). Are self-reported gastrointestinal symptoms among older adults associated with increased intestinal permeability and psychological distress?. BMC Geriatrics, 18(1), Article ID 75.
Åpne denne publikasjonen i ny fane eller vindu >>Are self-reported gastrointestinal symptoms among older adults associated with increased intestinal permeability and psychological distress?
Vise andre…
2018 (engelsk)Inngår i: BMC Geriatrics, ISSN 1471-2318, E-ISSN 1471-2318, Vol. 18, nr 1, artikkel-id 75Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

BACKGROUND: Despite the substantial number of older adults suffering from gastrointestinal (GI) symptoms little is known regarding the character of these complaints and whether they are associated with an altered intestinal barrier function and psychological distress. Our aim was to explore the relationship between self-reported gut health, intestinal permeability and psychological distress among older adults.

METHODS: Three study populations were included: 1) older adults with GI symptoms (n = 24), 2) a group of older adults representing the general elderly population in Sweden (n = 22) and 3) senior orienteering athletes as a potential model of healthy ageing (n = 27). Questionnaire data on gut-health, psychological distress and level of physical activity were collected. Intestinal permeability was measured by quantifying zonulin in plasma. The level of systemic and local inflammation was monitored by measuring C-reactive protein (CRP), hydrogen peroxide in plasma and calprotectin in stool samples. The relationship between biomarkers and questionnaire data in the different study populations was illustrated using a Principal Component Analysis (PCA).

RESULTS: Older adults with GI symptoms displayed significantly higher levels of both zonulin and psychological distress than both general older adults and senior orienteering athletes. The PCA analysis revealed a separation between senior orienteering athletes and older adults with GI symptoms and showed an association between GI symptoms, psychological distress and zonulin.

CONCLUSIONS: Older adults with GI symptoms express increased plasma levels of zonulin, which might reflect an augmented intestinal permeability. In addition, this group suffer from higher psychological distress compared to general older adults and senior orienteering athletes. This relationship was further confirmed by a PCA plot, which illustrated an association between GI symptoms, psychological distress and intestinal permeability.

sted, utgiver, år, opplag, sider
BioMed Central, 2018
Emneord
Older adults; Gastrointestinal symptoms; Intestinal barrier function; Psychological distress
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-66053 (URN)10.1186/s12877-018-0767-6 (DOI)000428260300001 ()29554871 (PubMedID)2-s2.0-85044174344 (Scopus ID)
Forskningsfinansiär
Knowledge Foundation, 20110225
Merknad

Funding Agencies:

Bo Rydins stiftelse  F0514 

Faculty of Medicine and Health at Örebro University  

Diarrheal Disease Research Centre, Linköping University  

Tilgjengelig fra: 2018-03-27 Laget: 2018-03-27 Sist oppdatert: 2019-12-20bibliografisk kontrollert
Ganda Mall, J. P., Löfvendahl, L., Lindqvist, C. M., Brummer, R. J., Keita, Å. V. & Schoultz, I. (2018). Differential effects of dietary fibres on colonic barrier function in elderly individuals with gastrointestinal symptoms. Scientific Reports, 8(1), Article ID 13404.
Åpne denne publikasjonen i ny fane eller vindu >>Differential effects of dietary fibres on colonic barrier function in elderly individuals with gastrointestinal symptoms
Vise andre…
2018 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, nr 1, artikkel-id 13404Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Gastrointestinal problems are common in elderly and often associated with psychological distress and increased levels of corticotrophin-releasing hormone, a hormone known to cause mast cell (MC) degranulation and perturbed intestinal barrier function. We investigated if dietary fibres (non-digestible polysaccharides [NPS]) could attenuate MC-induced colonic hyperpermeability in elderly with gastrointestinal (GI) symptoms. Colonic biopsies from elderly with diarrhoea and/or constipation (n = 18) and healthy controls (n = 19) were mounted in Ussing chambers and pre-stimulated with a yeast-derived beta (β)-glucan (0.5 mg/ml) or wheat-derived arabinoxylan (0.1 mg/ml) before the addition of the MC-degranulator Compound (C) 48/80 (10 ng/ml). Permeability markers were compared pre and post exposure to C48/80 in both groups and revealed higher baseline permeability in elderly with GI symptoms. β-glucan significantly attenuated C48/80-induced hyperpermeability in elderly with GI symptoms but not in healthy controls. Arabinoxylan reduced MC-induced paracellular and transcellular hyperpermeability across the colonic mucosa of healthy controls, but did only attenuate transcellular permeability in elderly with GI symptoms. Our novel findings indicate that NPS affect the intestinal barrier differently depending on the presence of GI symptoms and could be important in the treatment of moderate constipation and/or diarrhoea in elderly.

sted, utgiver, år, opplag, sider
Nature Publishing Group, 2018
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-68799 (URN)10.1038/s41598-018-31492-5 (DOI)000444022800008 ()30194322 (PubMedID)2-s2.0-85053008629 (Scopus ID)
Tilgjengelig fra: 2018-09-10 Laget: 2018-09-10 Sist oppdatert: 2019-12-20bibliografisk kontrollert
Ganda Mall, J.-P. (2018). Non-digestible Polysaccharides and Intestinal Barrier Function: specific focus on its efficacy in elderly and patients with Crohn’s disease. (Doctoral dissertation). Örebro: Örebro University
Åpne denne publikasjonen i ny fane eller vindu >>Non-digestible Polysaccharides and Intestinal Barrier Function: specific focus on its efficacy in elderly and patients with Crohn’s disease
2018 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

A large number of elderly suffer from gastrointestinal (GI) symptoms such as constipation and diarrhoea. The underlying mechanisms of age-acquired GI symptoms are not well studied but are necessary to clarify in order to recommend the right treatment. Non-digestible polysaccharides (NPS) are dietary fibres that could have beneficial effects on the intestinal immune system and barrier function, although their efficacy needs to be evaluated. Paper I showed that elderly with GI symptoms have significantly higher small intestinal permeability than a general elderly population, along with a stronger association to psychological distress. In Paper II we performed a randomised controlled trial with a general population of elderly that consumed either placebo, the NPS’s arabinoxylan or oat β-glucan for a period of 6 weeks. No protective effects were observed related to indomethacin-induced intestinal hyperpermeability, inflammatory markers, or self-reported health if compared to placebo. Paper III showed that stimulation with a yeast-derived β-glucan significantly attenuated Compound (C) 48/80-induced hyperpermeability in colonic biopsies from elderly with GI symptoms mounted in Ussing chambers, but not in young healthy adults. Arabinoxylan attenuated only C48/80-induced transcellular permeability in elderly but both paracellular and transcellular permeability in young healthy adults. Paper IV showed that the same yeast-derived β-glucan from paper III could cross the epithelium of ileal tissues from patients with Crohn’s disease (CD) and non-CD controls, mounted in Ussing chambers, and attenuate C48/80-induced hyperpermeability. In conclusion, we found that elderly with GI symptoms display a deteriorated barrier function and that administration of selective NPS can have beneficial effect on intestinal permeability in selective populations.

sted, utgiver, år, opplag, sider
Örebro: Örebro University, 2018. s. 121
Serie
Örebro Studies in Medicine, ISSN 1652-4063 ; 180
Emneord
Non-digestible polysachharides, beta-glucan, arabinoxylan, barrier function, permeability, Ussing chamber, elderly, Crohn’s disease
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-66055 (URN)978-91-7529-246-5 (ISBN)
Disputas
2018-06-08, Örebro universitet, Campus USÖ, hörsal C3, Södra Grev Rosengatan 32, Örebro, 13:00 (svensk)
Opponent
Veileder
Tilgjengelig fra: 2018-03-26 Laget: 2018-03-26 Sist oppdatert: 2018-05-08bibliografisk kontrollert
Ganda Mall, J.-P., Casado-Bedmar, M., Winberg, M. E., Brummer, R. J., Schoultz, I. & Keita, Å. V. (2017). A β-Glucan-Based Dietary Fiber Reduces Mast Cell-Induced Hyperpermeability in Ileum From Patients With Crohn's Disease and Control Subjects. Inflammatory Bowel Diseases, 24(1), 166-178
Åpne denne publikasjonen i ny fane eller vindu >>A β-Glucan-Based Dietary Fiber Reduces Mast Cell-Induced Hyperpermeability in Ileum From Patients With Crohn's Disease and Control Subjects
Vise andre…
2017 (engelsk)Inngår i: Inflammatory Bowel Diseases, ISSN 1078-0998, E-ISSN 1536-4844, Vol. 24, nr 1, s. 166-178Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Administration of β-glucan has shown immune-enhancing effects. Our aim was to investigate whether β-glucan could attenuate mast cell (MC)-induced hyperpermeability in follicle-associated epithelium (FAE) and villus epithelium (VE) of patients with Crohn's disease (CD) and in noninflammatory bowel disease (IBD)-controls. Further, we studied mechanisms of β-glucan uptake and effects on MCs in vitro.

Methods: Segments of FAE and VE from 8 CD patients and 9 controls were mounted in Ussing chambers. Effects of the MC-degranulator compound 48/80 (C48/80) and yeast-derived β-1,3/1,6 glucan on hyperpermeability were investigated. Translocation of β-glucan and colocalization with immune cells were studied by immunofluorescence. Caco-2-cl1- and FAE-cultures were used to investigate β-glucan-uptake using endocytosis inhibitors and HMC-1.1 to study effects on MCs.

Results: β-glucan significantly attenuated MC-induced paracellular hyperpermeability in CD and controls. Transcellular hyperpermeability was only significantly attenuated in VE. Baseline paracellular permeability was higher in FAE than VE in both groups, P<0.05, and exhibited a more pronounced effect by C48/80 and β-glucan P<0.05. No difference was observed between CD and controls. In vitro studies showed increased passage, P<0.05, of β-glucan through FAE-culture compared to Caco-2-cl1. Passage was mildly attenuated by the inhibitor methyl-β-cyclodextrin. HMC-1.1 experiments showed a trend to decreasing MC-degranulation and levels of TNF-α but not IL-6 by β-glucan. Immunofluorescence revealed more β-glucan-uptake and higher percentage of macrophages and dendritic cells close to β-glucan in VE of CD compared to controls.

Conclusions: We demonstrated beneficial effects of β-glucan on intestinal barrier function and increased β-glucan-passage through FAE model. Our results provide important and novel knowledge on possible applications of β-glucan in health disorders and diseases characterized by intestinal barrier dysfunction.

sted, utgiver, år, opplag, sider
Lippincott-Raven Publishers, 2017
Emneord
Crohn’s disease, intestinal permeability, β-glucan
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-63994 (URN)10.1093/ibd/izx002 (DOI)000427524400018 ()29272475 (PubMedID)
Forskningsfinansiär
Swedish Foundation for Strategic Research , RB13-016Swedish Research Council, 2014-02537
Merknad

Funding Agency:

LIONS research foundation

Tilgjengelig fra: 2018-01-09 Laget: 2018-01-09 Sist oppdatert: 2018-08-13bibliografisk kontrollert
Rangel, I., Ganda Mall, J. P., Roger, W., Sjöberg, F. & Hultgren-Hörnquist, E. (2016). Degree of colitis correlates with microbial composition and cytokine responses in colon and caecum of Gαi2-deficient mice. FEMS Microbiology Ecology, 92(7), Article ID fiw098.
Åpne denne publikasjonen i ny fane eller vindu >>Degree of colitis correlates with microbial composition and cytokine responses in colon and caecum of Gαi2-deficient mice
Vise andre…
2016 (engelsk)Inngår i: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 92, nr 7, artikkel-id fiw098Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

An altered immune response and gut microbiota have been associated with the pathology of Inflammatory Bowel Diseases (IBD). However, there is limited knowledge of how inflammation is associated with changes in the microbiota. We studied the microbiota in the intestine and faeces as well as the cytokine gene expressions in caecum and colon of a mouse model (Gαi2(-/-)) of colitis, and analysed them in relation to the degrees of inflammation in the colon. The degree of colitis was associated with general changes in the complexity of the microbiota and was corroborated by quantitative analyses of the Bacteroides and Lactobacillus High gene expression levels of IL-17 and IFN-γ in colon and caecum were detected in Gαi2(-/-) mice with moderate and severe colitis. High IL-27 gene expression in the colon of mice with moderate and severe colitis and in the caecum of mice with moderate colitis was also detected. Negative correlations between IL-27 and Bacteroides and Lactobacillus and between IFN-γ and Lactobacillus were detected in caecum. This research indicates that the degree of colitis in IBD correlates with the gene expression of cytokines and with disturbances in the gut microbiota. Furthermore, the caecum could have an important role in the pathology of IBD.

sted, utgiver, år, opplag, sider
Oxford, United Kingdom: Oxford University Press, 2016
Emneord
Microbiota, gut, IBD, cytokines, colon, caecum
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-50318 (URN)10.1093/femsec/fiw098 (DOI)000379693700009 ()27162181 (PubMedID)2-s2.0-84978438318 (Scopus ID)
Tilgjengelig fra: 2016-05-27 Laget: 2016-05-16 Sist oppdatert: 2018-07-10bibliografisk kontrollert
König, J., Ganda-Mall, J.-P., Rangel, I., Edebol-Carlman, H. & Brummer, R. J. (2015). The Role of the Gut Microbiota in Brain Function. In: Koen Venema & Ana Paula do Carmo (Ed.), Probiotics and Prebiotics: Current Research and Future Trends. Poole, UK: Caister Academic Press
Åpne denne publikasjonen i ny fane eller vindu >>The Role of the Gut Microbiota in Brain Function
Vise andre…
2015 (engelsk)Inngår i: Probiotics and Prebiotics: Current Research and Future Trends / [ed] Koen Venema & Ana Paula do Carmo, Poole, UK: Caister Academic Press, 2015Kapittel i bok, del av antologi (Fagfellevurdert)
sted, utgiver, år, opplag, sider
Poole, UK: Caister Academic Press, 2015
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-39860 (URN)978-1-910190-09-8 (ISBN)
Tilgjengelig fra: 2014-12-17 Laget: 2014-12-17 Sist oppdatert: 2019-03-26bibliografisk kontrollert
Rangel, I., Ganda-Mall, J.-P., Sjöberg, F., Willen, R. & Hultgren-Hörnquist, E. (2013). Alterations in gut microbiota composition in tissues and feces of G alpha i2(-/-) mice with colitis in parallel with enhanced cytokine secretion. Paper presented at Annual Congress of the British-Society-for-Immunology, Liverpool, ENGLAND, DEC 02-05, 2013. Immunology, 140, 168-169
Åpne denne publikasjonen i ny fane eller vindu >>Alterations in gut microbiota composition in tissues and feces of G alpha i2(-/-) mice with colitis in parallel with enhanced cytokine secretion
Vise andre…
2013 (engelsk)Inngår i: Immunology, ISSN 0019-2805, E-ISSN 1365-2567, Vol. 140, s. 168-169Artikkel i tidsskrift, Meeting abstract (Annet vitenskapelig) Published
sted, utgiver, år, opplag, sider
Hoboken, USA: Wiley-Blackwell, 2013
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-32913 (URN)000327487700442 ()
Eksternt samarbeid:
Konferanse
Annual Congress of the British-Society-for-Immunology, Liverpool, ENGLAND, DEC 02-05, 2013
Tilgjengelig fra: 2014-01-03 Laget: 2014-01-03 Sist oppdatert: 2017-12-06bibliografisk kontrollert
Ganda Mall, J.-P., Löfvendahl, L., Brummer, R. J., Keita, Å. V. & Schoultz, I.Differential effects of dietary fibres on colonic barrier function in elderly individuals with gastrointestinal symptoms.
Åpne denne publikasjonen i ny fane eller vindu >>Differential effects of dietary fibres on colonic barrier function in elderly individuals with gastrointestinal symptoms
Vise andre…
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-66866 (URN)
Tilgjengelig fra: 2018-05-04 Laget: 2018-05-04 Sist oppdatert: 2018-05-04bibliografisk kontrollert
Ganda Mall, J.-P., Fart, F., Sabet, J., Lindqvist, C.-M., Keita, Å. V., Brummer, R. J. & Schoultz, I.Effects of dietary fibres on indomethacin-induced intestinal permeability in elderly: A randomised placebo controlled parallel clinical trial.
Åpne denne publikasjonen i ny fane eller vindu >>Effects of dietary fibres on indomethacin-induced intestinal permeability in elderly: A randomised placebo controlled parallel clinical trial
Vise andre…
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Identifikatorer
urn:nbn:se:oru:diva-66863 (URN)
Tilgjengelig fra: 2018-05-04 Laget: 2018-05-04 Sist oppdatert: 2019-12-20bibliografisk kontrollert
Östlund-Lagerström, L., Ganda Mall, J.-P., Algilani, S., Rasoal, D., Brummer, R. J. & Schoultz, I.Low levels of inflammation and oxidative stress in senior orienteering athletes.
Åpne denne publikasjonen i ny fane eller vindu >>Low levels of inflammation and oxidative stress in senior orienteering athletes
Vise andre…
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Forskningsprogram
Medicin
Identifikatorer
urn:nbn:se:oru:diva-49596 (URN)
Tilgjengelig fra: 2016-03-31 Laget: 2016-03-31 Sist oppdatert: 2018-01-10bibliografisk kontrollert
Organisasjoner