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Allbrand, Marianne
Publikationer (4 of 4) Visa alla publikationer
Allbrand, M., Åman, J., Nilsson, K., Cao, Y. & Lodefalk, M. (2019). Expression of genes involved in inflammation and growth: does sampling site in human full-term placenta matter?. Journal of Perinatal Medicine, 47(5), 539-546
Öppna denna publikation i ny flik eller fönster >>Expression of genes involved in inflammation and growth: does sampling site in human full-term placenta matter?
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2019 (Engelska)Ingår i: Journal of Perinatal Medicine, ISSN 0300-5577, E-ISSN 1619-3997, Vol. 47, nr 5, s. 539-546Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Objective: To investigate the placental gene expression of substances in the inflammatory cascade and growth factors at nine different well-defined sampling sites in full-term placentas from 12 normal weight healthy non-smoking women with an uncomplicated singleton pregnancy.

Methods: All placentas (six girls and six boys) were delivered vaginally. Quantitative real-time polymerase chain reaction was used to analyze toll receptor-2 and -4, interleukin-6 and -8, tumor necrosis factor-α, leptin, ghrelin, insulin-like growth factor-1 and -2, hepatocyte growth factor, hepatocyte growth factor receptor and insulin receptor (IR).

Results: The leptin gene and the IR gene showed higher expression in lateral regions near the chorionic plate compared to central regions near the basal plate (P = 0.028 and P = 0.041, respectively).

Conclusion: Our results suggest that the sampling site may influence the gene expression for leptin and IR in placental tissue obtained from full-term normal pregnancies. We speculate that this may be due to differences in placental structure and perfusion and may be important when future studies are designed.

Ort, förlag, år, upplaga, sidor
Walter de Gruyter, 2019
Nyckelord
Cytokines, gene expression, growth factors, placenta, sampling
Nationell ämneskategori
Reproduktionsmedicin och gynekologi
Identifikatorer
urn:nbn:se:oru:diva-73427 (URN)10.1515/jpm-2018-0290 (DOI)000473532900008 ()30920955 (PubMedID)2-s2.0-85063721684 (Scopus ID)
Anmärkning

Funding Agencies:

Research Committee of Region Örebro County  

Nyckelfonden, Örebro University Hospital  

Tillgänglig från: 2019-04-04 Skapad: 2019-04-04 Senast uppdaterad: 2019-08-08Bibliografiskt granskad
Allbrand, M., Åman, J. & Lodefalk, M. (2017). Placental ghrelin and leptin expression and cord blood ghrelin, adiponectin, leptin, and C-peptide levels in severe maternal obesity. The Journal of Maternal-Fetal & Neonatal Medicine, 31(21), 2839-2846
Öppna denna publikation i ny flik eller fönster >>Placental ghrelin and leptin expression and cord blood ghrelin, adiponectin, leptin, and C-peptide levels in severe maternal obesity
2017 (Engelska)Ingår i: The Journal of Maternal-Fetal & Neonatal Medicine, ISSN 1476-7058, E-ISSN 1476-4954, Vol. 31, nr 21, s. 2839-2846Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

PURPOSE: The purpose of this study is to investigate placental ghrelin and leptin expression as well as cord blood ghrelin and adiponectin levels in maternal obesity and associations between placental ghrelin expression, cord blood ghrelin levels and maternal and infant variables.

MATERIALS AND METHODS: Placental ghrelin and leptin expression were analyzed by RT-PCR in 32 severely obese and 32 matched normal-weight women. Cord blood ghrelin, adiponectin, leptin, and C-peptide concentrations were analyzed by ELISA.

RESULTS: Neither ghrelin nor leptin expression and neither cord blood ghrelin nor adiponectin levels differed between the groups. Placental ghrelin expression was associated with BMI at delivery in the obese women (r = 0.424, p = .016) and in the infants born to normal-weight women with their weight z-scores at six (r = -0.642, p = .010), nine (r = -0.441, p = .015), and 12 months of age (r = -0.402, p = .028).

CONCLUSIONS: Placental ghrelin and leptin expression as well as cord blood ghrelin and adiponectin levels do not seem to be altered in severe maternal obesity. Placenta-derived ghrelin may influence the infants' postnatal weight gain, but possibly only when the mother has normal weight.

Ort, förlag, år, upplaga, sidor
Taylor & Francis Group, 2017
Nyckelord
Adiponectin, birth weight, ghrelin, leptin, obesity, placenta
Nationell ämneskategori
Reproduktionsmedicin och gynekologi
Identifikatorer
urn:nbn:se:oru:diva-61734 (URN)10.1080/14767058.2017.1358262 (DOI)000440610300007 ()28783996 (PubMedID)2-s2.0-85027047254 (Scopus ID)
Anmärkning

Funding agencies:

Research Committee of Region Örebro County

Nyckelfonden, Örebro University Hospital

Tillgänglig från: 2017-11-06 Skapad: 2017-11-06 Senast uppdaterad: 2018-09-12Bibliografiskt granskad
Allbrand, M., Åman, J. & Lodefalk, M. (2015). Adipocytokines in placenta and cord blood in relation to maternal obesity, and foetal and postnatal growth of the child. Paper presented at 54th Annual ESPE (European Society for Paediatric Endocrinology) Meeting in Barcelona, Spain, 1-3 October 2015.. Hormone Research in Paediatrics, 82(Suppl. 1), 47-48
Öppna denna publikation i ny flik eller fönster >>Adipocytokines in placenta and cord blood in relation to maternal obesity, and foetal and postnatal growth of the child
2015 (Engelska)Ingår i: Hormone Research in Paediatrics, ISSN 1663-2818, E-ISSN 1663-2826, Vol. 82, nr Suppl. 1, s. 47-48Artikel i tidskrift, Meeting abstract (Övrigt vetenskapligt) Published
Abstract [en]

Background: The nutritional and hormonal state in utero may be a link between maternal obesity and obesity in the offspring. The gene expression in placentae in pregnancies complicated by diabetes is reduced for leptin, but increased for ghrelin. It is not known whether these genes’ expressions in placentae are altered in maternal obesity.

Objectives and hypotheses: To compare obese and normal-weight women and their children concerning gene expressions of leptin and ghrelin in placentae; leptin, ghrelin, adiponectin, and C-peptide levels in cord blood, birth size and postnatal growth. Changes in the expression of these adipocytokines may lead to an altered hypothalamic sensitivity to leptin and ghrelin resulting in an increased risk of obesity in the offspring.

Method: 32 women with pre-pregnancy obesity, but otherwise healthy, were compared to 32 matched, normal-weight controls. Full-term placenta biopsies were analysed with qPCR for leptin mRNA and ghrelin mRNA. Cord blood samples were examined with ELISA for leptin, ghrelin, adiponectin, and C-peptide concentrations. Birth size and postnatal growth of the children were collected from clinical registers at the Child Health Care Units.

Results: The leptin and ghrelin gene expressions in placentae did not differ between obese and normal-weight women. The leptin concentration in cord blood was higher in children of obese mothers (P=0.021). It correlated with birth weight Z-score (r=0.467, P<0.001) and C-peptide level in cord blood (r=0.446, P<0.001). Children of obese women were slightly heavier at birth, but postnatal growth did not differ between groups. Children with birth weight  ≤−0.67 Z-score had higher ghrelin levels in cord blood than heavier children (P=0.042). The leptin level in cord blood correlated negatively with weight gain at 6 months (r=−0.332, P=0.009). The ghrelin level in cord blood correlated with weight gain at 3 months in girls (r=0.611, P=0.001), but not in boys. The adiponectin level in cord blood correlated negatively with length gain at 3 years in the obese group (r=−0.571, P=0.033), but not in the normal-weight group.

Conclusion: Leptin and ghrelin placental gene expressions are not altered in obese women, but foetal adipocytokine production may influence early postnatal growth, possibly by influencing hunger signalling or insulin levels

Ort, förlag, år, upplaga, sidor
Basel, Switzerland: S. Karger, 2015
Nyckelord
Obesity, ghrelin, leptin, adipocytokines, growth
Nationell ämneskategori
Medicin och hälsovetenskap Pediatrik
Forskningsämne
Pediatrik
Identifikatorer
urn:nbn:se:oru:diva-49275 (URN)
Konferens
54th Annual ESPE (European Society for Paediatric Endocrinology) Meeting in Barcelona, Spain, 1-3 October 2015.
Tillgänglig från: 2016-03-11 Skapad: 2016-03-11 Senast uppdaterad: 2017-11-30Bibliografiskt granskad
Allbrand, M., Björkqvist, M., Nilsson, K., Östlund, I. & Åman, J. (2015). Placental gene expression of inflammatory markers and growth factors: a case control study of obese and normal weight women. Journal of Perinatal Medicine, 43(2), 159-164
Öppna denna publikation i ny flik eller fönster >>Placental gene expression of inflammatory markers and growth factors: a case control study of obese and normal weight women
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2015 (Engelska)Ingår i: Journal of Perinatal Medicine, ISSN 0300-5577, E-ISSN 1619-3997, Vol. 43, nr 2, s. 159-164Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Objective: To survey the placental gene expression of inflammatory markers and growth factors in non-smoking obese women with an uncomplicated pregnancy without associated morbidity and delivery at term compared with normal weight women.

Methods: Placental tissue samples from 32 obese women (body mass index, BMI >= 35.0 kg/m(2)) were compared with samples from 94 normal weight women (BMI 18.5-25.0 kg/m(2)) matched for age (+/- 1 year), gestational age (+/- 3 days), parity and mode of delivery. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to analyse toll receptor-2 and -4, interleukin-6 and -8, tumour necrosis factor-alpha, leptin, adiponectin, insulin-like growth factor-1 and -2, hepatocyte growth factor, hepatocyte growth factor receptor and insulin receptor.

Results: There was no significant difference in gene expression in placental tissue samples from obese and normal weight women.

Conclusion: We found no difference in the occurrence of inflammatory marker and growth factor mRNA levels in placental tissue samples from a large group of obese women without associated morbidity and with healthy infants compared to a closely matched control group of healthy normal weight women. Compared with the previous studies, this anomalous finding may be explained by the absence of associated morbidity in the obese women in our study.

Ort, förlag, år, upplaga, sidor
Walter de Gruyter, 2015
Nyckelord
Obesity, pregnancy
Nationell ämneskategori
Reproduktionsmedicin och gynekologi Pediatrik
Forskningsämne
Obstetrik och gynekologi; Pediatrik
Identifikatorer
urn:nbn:se:oru:diva-44240 (URN)10.1515/jpm-2013-0343 (DOI)000350338000005 ()25014513 (PubMedID)
Anmärkning

Funding Agency:

Foundation for Medical Research, Orebro University Hospital

Tillgänglig från: 2015-04-14 Skapad: 2015-04-14 Senast uppdaterad: 2018-06-29Bibliografiskt granskad
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