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Folkesson, M., Mackey, A. L., Langberg, H., Oskarsson, E., Piehl-Aulin, K., Henriksson, J. & Kadi, F. (2013). The expression of heat shock protein in human skeletal muscle: effects of muscle fibre phenotype and training background. Acta Physiologica, 209(1), 26-33
Öppna denna publikation i ny flik eller fönster >>The expression of heat shock protein in human skeletal muscle: effects of muscle fibre phenotype and training background
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2013 (Engelska)Ingår i: Acta Physiologica, ISSN 1748-1708, E-ISSN 1748-1716, Vol. 209, nr 1, s. 26-33Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Aim: Exercise-induced adaptations of skeletal muscle are related to training mode and can be muscle fibre type specific. This study aimed to investigate heat shock protein expression in type I and type II muscle fibres in resting skeletal muscle of subjects with different training backgrounds.

Methods: Three groups of subjects were included: healthy active not engaged in any training programme (ACT, n = 12), resistance trained (RES, n = 6) and endurance trained (END, n = 8). Biopsies were obtained from vastus lateralis, and immunohistochemistry was performed using monoclonal antibodies against myosin heavy chain I and IIA, αB-crystallin, HSP27, HSP60 and HSP70.

Results: In ACT and RES, but not in END, a fibre type–specific expression with higher staining intensity in type I than type II fibres was seen for αB-crystallin. The opposite (II > I) was found for HSP27 in subjects from ACT (6 of 12 subjects) and RES (3 of 6), whereas all subjects from END displayed uniform staining. HSP60 showed no fibre-specific expression. HSP70 displayed a fibre-specific expression pattern (I > II) in ACT (4 of 12), but not in END or RES.

Conclusion: This study shows that the level of expression of the different HSPs in human skeletal muscle is influenced by muscle fibre phenotype. The fibre type–specific expression of HSP70 is influenced by resistance and endurance training, whereas those of αB-crystallin and HSP27 is influenced only by endurance training, suggesting the existence of a training-modality-specific action on the adaptive processes including heat shock proteins in human skeletal muscle.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell, 2013
Nyckelord
Adaptation, endurance, heat shock protein, immunohistochemistry, resistance
Nationell ämneskategori
Idrottsvetenskap
Forskningsämne
Idrott med inriktning mot fysiologi
Identifikatorer
urn:nbn:se:oru:diva-30127 (URN)10.1111/apha.12124 (DOI)000322950400006 ()23710799 (PubMedID)2-s2.0-84881557932 (Scopus ID)
Anmärkning

Funding agency:

Nordea Foundation

Tillgänglig från: 2013-08-06 Skapad: 2013-08-06 Senast uppdaterad: 2018-11-19Bibliografiskt granskad
Eliason, G., Zakrisson, A.-B., Piehl-Aulin, K. & Hurtig-Wennlöf, A. (2011). Physical activity patterns in patients in different stages of chronic obstructive pulmonary disease. COPD: Journal of Chronic Obstructive Pulmonary Disease, 8(5), 369-374
Öppna denna publikation i ny flik eller fönster >>Physical activity patterns in patients in different stages of chronic obstructive pulmonary disease
2011 (Engelska)Ingår i: COPD: Journal of Chronic Obstructive Pulmonary Disease, ISSN 1541-2555, E-ISSN 1541-2563, Vol. 8, nr 5, s. 369-374Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

It has previously been suggested that exercise capacity is decreased in COPD and that it is associated with degree of disease. The reduced exercise capacity may plausibly be due to low levels of physical activity in this patient group. The aim of the present study was to assess exercise capacity and physical activity in different stages of COPD and to examine the associations between exercise capacity, pulmonary function and degree of physical activity. A total of 44 COPD patients and 17 healthy subjects participated in the study. Exercise capacity was assessed using the 6-minute walking test and physical activity was assessed using an accelerometer worn all waking hours during 7 days. Mean exercise capacity was significantly lower in COPD patients compared with healthy subjects. Mean physical activity level and time spent at least moderately active were significantly lower in patients with moderate and severe COPD compared with healthy subjects while no differences in time spent sedentary were observed between the study groups. Pulmonary function, mean physical activity level and time spent at least moderately physically active were significantly associated with exercise capacity in the patients. We conclude that patients with moderate and severe COPD are less physically active compared with healthy subjects. Furthermore, mean physical activity level and physical activity of at least moderate intensity are positively associated with exercise capacity in COPD, while time spent sedentary is not, which stresses an important role of physical activity on exercise capacity in these patients.

Nyckelord
COPD, physical activity, accelerometry, exercise capacity
Nationell ämneskategori
Medicin och hälsovetenskap
Forskningsämne
Medicin
Identifikatorer
urn:nbn:se:oru:diva-20846 (URN)10.3109/15412555.2011.605403 (DOI)000295081900008 ()
Tillgänglig från: 2012-01-11 Skapad: 2012-01-11 Senast uppdaterad: 2017-12-08Bibliografiskt granskad
Eliason, G., Abdel-Halim, S. M., Piehl-Aulin, K. & Kadi, F. (2010). Alterations in the muscle-to-capillary interface in patients with different degrees of chronic obstructive pulmonary disease. Respiratory research (Online), 11, Article ID 97.
Öppna denna publikation i ny flik eller fönster >>Alterations in the muscle-to-capillary interface in patients with different degrees of chronic obstructive pulmonary disease
2010 (Engelska)Ingår i: Respiratory research (Online), ISSN 1465-9921, E-ISSN 1465-993X, Vol. 11, artikel-id 97Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: It is hypothesized that decreased capillarization of limb skeletal muscle is implicated in the decreased exercise tolerance in COPD patients. We have recently demonstrated decreased number of capillaries per muscle fibre (CAF) but no changes in CAF in relation to fibre area (CAFA), which is based on the diffusion distance between the capillary and muscle fibre. The aim of the current study is to investigate the muscle-to-capillary interface which is an important factor involved in oxygen supply to the muscle that has previously been suggested to be a more sensitive marker for changes in the capillary bed compared to CAF and CAFA.

Methods: 23 COPD patients and 12 age-matched healthy subjects participated in the study. Muscle-to-capillary interface was assessed in muscle biopsies from the tibialis anterior muscle using the following parameters:

1) The capillary-to-fibre ratio (C:Fi) which is defined as the sum of the fractional contributions of all capillary contacts around the fibre

2) The ratio between C:Fi and the fibre perimeter (CFPE-index)

3) The ratio between length of capillary and fibre perimeter (LC/PF) which is also referred to as the index of tortuosity.

Exercise capacity was determined using the 6-min walking test.

Results: A positive correlation was found between CFPE-index and ascending disease severity with CFPE-index for type I fibres being significantly lower in patients with moderate and severe COPD. Furthermore, a positive correlation was observed between exercise capacity and CFPE-index for both type I and type IIa fibres.

Conclusion: It can be concluded that the muscle-to-capillary interface is disturbed in the tibialis anterior muscle in patients with COPD and that interface is strongly correlated to increased disease severity and to decreased exercise capacity in this patient group.

 

Ort, förlag, år, upplaga, sidor
London, United Kingdom: BioMed Central, 2010
Nyckelord
COPD, muscle-capillary interface, CFPE-index, LC/PF, tortuosity
Nationell ämneskategori
Fysiologi Medicin och hälsovetenskap
Forskningsämne
Fysiologi; Medicin
Identifikatorer
urn:nbn:se:oru:diva-12493 (URN)10.1186/1465-9921-11-97 (DOI)000282550500002 ()20633289 (PubMedID)2-s2.0-77956692758 (Scopus ID)
Tillgänglig från: 2010-11-19 Skapad: 2010-11-18 Senast uppdaterad: 2018-09-11Bibliografiskt granskad
Nilsson, A., Andersen, L. B., Ommundsen, Y., Froberg, K., Sardinha, L. B., Piehl-Aulin, K. & Ekelund, U. (2009). Correlates of objectively assessed physical activity and sedentary time in children: a cross-sectional study (The European Youth Heart Study). BMC Public Health, 9, Article ID 322.
Öppna denna publikation i ny flik eller fönster >>Correlates of objectively assessed physical activity and sedentary time in children: a cross-sectional study (The European Youth Heart Study)
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2009 (Engelska)Ingår i: BMC Public Health, ISSN 1471-2458, E-ISSN 1471-2458, Vol. 9, artikel-id 322Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background

Identifying leisure time activities performed before and after school that influence time in physical activity (PA) and/or time spent sedentary can provide useful information when designing interventions aimed to promote an active lifestyle in young people. The purpose of this study was to examine associations between mode of transportation to school, outdoor play after school, participation in exercise in clubs, and TV viewing with objectively assessed PA and sedentary behaviour in children.

Methods

A total of 1327 nine- and 15-year-old children from three European countries (Norway, Estonia, Portugal) participated as part of the European Youth Heart Study. PA was measured during two weekdays and two weekend days using the MTI accelerometer, and average percent of time in moderate-to-vigorous PA (MVPA) and time spent sedentary were derived. Potential correlates were assessed by self-report. Independent associations between self-reported correlates with percent time in MVPA and percent time sedentary were analysed by general linear models, adjusted by age, gender, country, measurement period, monitored days and parental socio-economic status.

Results

In 9-year-olds, playing outdoors after school was associated with higher percent time in MVPA (P < 0.01), while participation in sport clubs was associated with higher percent time in MVPA (P < 0.01) in 15-year-olds. No associations with percent time sedentary were observed in either age group.

Conclusion

Frequency of outdoor play after school is a significant correlate for daily time in MVPA in 9-year-olds, while this correlate is attenuated in favour of participation in sport and exercise in clubs in 15-year-olds. Targeting walking to school or reduced TV viewing time in order to increase time in daily MVPA in children is unlikely to be sufficient. Correlates related to time spent sedentary need further examination.

Ort, förlag, år, upplaga, sidor
London: BioMed Central, 2009
Nyckelord
assessment, accelerometer, children, health behaviours
Nationell ämneskategori
Tvärvetenskapliga studier inom samhällsvetenskap Idrottsvetenskap Medicin och hälsovetenskap
Forskningsämne
Idrottsvetenskap; Biomedicin
Identifikatorer
urn:nbn:se:oru:diva-2878 (URN)10.1186/1471-2458-9-322 (DOI)000270675100001 ()19735565 (PubMedID)2-s2.0-70349311819 (Scopus ID)
Tillgänglig från: 2009-06-03 Skapad: 2009-05-25 Senast uppdaterad: 2019-09-20Bibliografiskt granskad
Oskarsson, E., Piehl Aulin, K., Gustafsson, B.-E. & Pettersson, K. (2009). Improved intramuscular blood flow and normalized metabolism in lateral epicondylitis after botulinum toxin treatment. Scandinavian Journal of Medicine and Science in Sports, 19(3), 323-328
Öppna denna publikation i ny flik eller fönster >>Improved intramuscular blood flow and normalized metabolism in lateral epicondylitis after botulinum toxin treatment
2009 (Engelska)Ingår i: Scandinavian Journal of Medicine and Science in Sports, ISSN 0905-7188, E-ISSN 1600-0838, Vol. 19, nr 3, s. 323-328Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Lateral epicondylitis is a common cause of elbow pain, and decreased microcirculation in extensor carpi radialis brevis (ECRB) has recently been suggested to contribute to the symptoms. The purpose of this pilot study was to investigate the treatment response after injection of botulinum toxin type A. Ten patients with unilateral epicondylitis and decreased intramuscular blood flow in ECRB participated. Handgrip, 2-pinch grip and muscle strength during radial deviation and dorsal extension of the wrist were recorded. Perceived pain during contraction was evaluated with the Visual Analogue Scale (VAS) and function in daily activities was assessed using the Disability of Arm, Shoulder and Hand instrument (DASH) and the Canadian Occupational Performance Measure instrument (COPM). Intramuscular blood flow was recorded by laser Doppler flowmetry, and microdialysis was used to analyze muscle metabolism. The difference in intramuscular blood flow between the control and the affected side had decreased 3 and 12 months after treatment (P=0.03). Lactate concentration at the 12-month follow-up had decreased (P=0.02); perceived pain was reduced and function in daily activities had improved. Injection of botulinum toxin is an alternative treatment for epicondylitis. Symptom relief may be due to enhanced microcirculation causing an aerobic metabolism.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell Publishing Inc., 2009
Nationell ämneskategori
Medicin och hälsovetenskap Idrottsvetenskap
Forskningsämne
Medicin; Idrottsvetenskap
Identifikatorer
urn:nbn:se:oru:diva-4607 (URN)10.1111/j.1600-0838.2008.00804.x (DOI)000266431300004 ()18435689 (PubMedID)2-s2.0-66249083500 (Scopus ID)
Tillgänglig från: 2008-09-23 Skapad: 2008-09-23 Senast uppdaterad: 2017-12-14Bibliografiskt granskad
Piehl-Aulin, K., Jones, I., Lindvall, B., Magnuson, A. & Abdel-Halim, S. M. (2009). Increased serum inflammatory markers in the absence of clinical and skeletal muscle inflammation in patients with chronic obstructive pulmonary disease. Respiration, 78(2), 191-196
Öppna denna publikation i ny flik eller fönster >>Increased serum inflammatory markers in the absence of clinical and skeletal muscle inflammation in patients with chronic obstructive pulmonary disease
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2009 (Engelska)Ingår i: Respiration, ISSN 0025-7931, E-ISSN 1423-0356, Vol. 78, nr 2, s. 191-196Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Muscle wasting and cachexia are common occurrences in patients with chronic obstructive pulmonary disease (COPD). Objectives: The current study aimed to investigate markers of inflammation in the circulation and skeletal muscle that might be associated with development of muscle wasting. Methods: Three groups of patients with mild, moderate and severe COPD and matched healthy controls were recruited. Serum levels of C-reactive protein (CRP), high-sensitivity CRP (hs-CRP), IL-6, IL-8, TNF-α, cortisol, insulin-like growth factor 1 (IGF-1), leptin and ghrelin were analysed. Skeletal muscle inflammation was investigated microscopically using a panel of antibodies and standard staining for inflammatory cell infiltration. Results: All COPD patients were clinically stable, with no sign of inflammation and normal CRP values. Compared to controls, significantly increased hs-CRP levels were observed in all COPD patient groups. Significant rises in IL-6 levels were first observed in moderate COPD, while IL-8 levels were significantly elevated at the late severe stage. Circulating levels of TNF-α, cortisol, IGF-1, leptin and ghrelin were similar to control levels. No microscopic signs of skeletal muscle inflammation were observed. Conclusion: Our results identify hs-CRP as an early marker of inflammation that is significantly increased in the circulation even in mild COPD. Serum interleukin levels appear to be increased with disease progress. These changes were manifested in the absence of any clinical signs of disease exacerbation, evidence of skeletal muscle inflammation or hormonal changes. © 2009 S. Karger AG, Basel.

Nyckelord
Chronic obstructive pulmonary disease, High sensitivity C-reactive protein, Inflammation, skeletal muscle
Nationell ämneskategori
Medicin och hälsovetenskap
Forskningsämne
Medicin
Identifikatorer
urn:nbn:se:oru:diva-14216 (URN)10.1159/000207793 (DOI)
Tillgänglig från: 2011-01-25 Skapad: 2011-01-25 Senast uppdaterad: 2018-04-16Bibliografiskt granskad
Branth, S., Hambraeus, L., Piehl-Aulin, K., Essén-Gustavsson, B., Åkerfeldt, T., Olsson, R., . . . Ronquist, G. (2009). Metabolic stress-like condition can be induced by prolonged strenuous exercise in athletes. Upsala Journal of Medical Sciences, 114(1), 12-25, Article ID PII 908457901.
Öppna denna publikation i ny flik eller fönster >>Metabolic stress-like condition can be induced by prolonged strenuous exercise in athletes
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2009 (Engelska)Ingår i: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 114, nr 1, s. 12-25, artikel-id PII 908457901Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Few studies have examined energy metabolism during prolonged, strenuous exercise. We wanted therefore to investigate energy metabolic consequences of a prolonged period of continuous strenuous work with very high energy expenditure. Twelve endurance-trained athletes (6 males and 6 females) were recruited. They performed a 7-h bike race on high work-load intensity. Physiological, biochemical, endocrinological, and anthropometric muscular compartment variables were monitored before, during, and after the race. The energy expenditure was high, being 5557 kcal. Work-load intensity (% of VO2 peak) was higher in females (77.7%) than in men (69.9%). Muscular glycogen utilization was pronounced, especially in type I fibres (>90%). Additionally, muscular triglyceride lipolysis was considerably accelerated. Plasma glucose levels were increased concomitantly with an unchanged serum insulin concentration which might reflect an insulin resistance state in addition to proteolytic glyconeogenesis. Increased reactive oxygen species (malondialdehyde (MDA)) were additional signs of metabolic stress. MDA levels correlated with glycogen utilization rate. A relative deficiency of energy substrate on a cellular level was indicated by increased intracellular water of the leg muscle concomitantly with increased extracellular levels of the osmoregulatory amino acid taurine. A kindred nature of a presumed insulin-resistant state with less intracellular availability of glucose for erythrocytes was also indicated by the findings of decreased MCV together with increased MCHC (haemoconcentration) after the race. This strenuous energy-demanding work created a metabolic stress-like condition including signs of insulin resistance and deteriorated intracellular glucose availability leading to compromised fuelling of ion pumps, culminating in a disturbed cellular osmoregulation indicated by taurine efflux and cellular swelling. © 2009 Informa UK Ltd All rights reserved.

Ort, förlag, år, upplaga, sidor
Taylor & Francis, 2009
Nyckelord
Cellular swelling, Energy expenditure, Lipid peroxidation, Metabolism, Myocytes, Taurine efflux
Nationell ämneskategori
Medicin och hälsovetenskap
Forskningsämne
Medicin
Identifikatorer
urn:nbn:se:oru:diva-14218 (URN)10.1080/03009730802579778 (DOI)000263095500003 ()19242868 (PubMedID)2-s2.0-65249102915 (Scopus ID)
Tillgänglig från: 2011-01-25 Skapad: 2011-01-25 Senast uppdaterad: 2017-12-11Bibliografiskt granskad
Jatta, K., Eliason, G., Portela-Gomes, G. M., Grimelius, L., Caro, O., Nilholm, L., . . . Abdel-Halim, S. M. (2009). Overexpression of von Hippel-Lindau protein in skeletal muscles of patients with chronic obstructive pulmonary disease. Journal of Clinical Pathology, 62(1), 70-76
Öppna denna publikation i ny flik eller fönster >>Overexpression of von Hippel-Lindau protein in skeletal muscles of patients with chronic obstructive pulmonary disease
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2009 (Engelska)Ingår i: Journal of Clinical Pathology, ISSN 0021-9746, E-ISSN 1472-4146, Vol. 62, nr 1, s. 70-76Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background/aim: A Significant number of patients with chronic obstructive pulmonary disease (COPD) exhibit skeletal muscle wasting and decreased capillary area formation which have been correlated to increased mortality. The current study aimed to determine the molecular mechanisms mediating decreased capillary formation in COPD.

Methods: Twenty-four COPD patients and twelve matching controls were recruited. COPD patients were divided into mild, moderate and severe groups according to GOLD (Global Initiative for Chronic Obstructive Lung Disease) criteria. Skeletal muscle biopsies were obtained from the tibialis anterior muscle. Fibre typing and capillary formation together with messenger RNA (mRNA) expression of hypoxia-inducible factors (HIF-1á and HIF-3á ), vascular endothelial growth factors (VEGF-A, -B and -C isoforms) and von Hippel Lindau (VHL) were determined. VHL expression and localization was further studied by immunohistochemistry.

Results: Skeletal muscle capillary formation was significantly decreased with ascending disease severity. Compared to controls, a tendency to mRNA overexpression of HIF-1á, HIF-3á and VEGF isoforms was observed at mild and moderate COPD that decreased at the severe stage. By contrast, skeletal muscle biopsies from COPD patients exhibited significant overexpression of VHL both on the mRNA and protein levels by immunohistochemistry. VHL protein was further determined to be localized to satellite cells.

Conclusions: Overexpression of VHL was identified in the skeletal muscle of patients with COPD. Increased VHL activity may exert a negative impact on transducing the hypoxic signal and may contribute to decreased capillarization in skeletal muscles of patients with COPD.

Ort, förlag, år, upplaga, sidor
London: BMJ Publishing Group Ltd, 2009
Nationell ämneskategori
Medicin och hälsovetenskap Cell- och molekylärbiologi
Forskningsämne
Patologi; Medicin
Identifikatorer
urn:nbn:se:oru:diva-3418 (URN)10.1136/jcp.2008.057190 (DOI)000262420400018 ()18818266 (PubMedID)2-s2.0-58349102711 (Scopus ID)
Tillgänglig från: 2008-12-04 Skapad: 2008-12-04 Senast uppdaterad: 2018-01-13Bibliografiskt granskad
Eliason, G., Abdel-Halim, S., Arvidsson, B., Kadi, F. & Piehl-Aulin, K. (2009). Physical performance and muscular characteristics in different stages of COPD. Scandinavian Journal of Medicine and Science in Sports, 19(6), 865-870
Öppna denna publikation i ny flik eller fönster >>Physical performance and muscular characteristics in different stages of COPD
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2009 (Engelska)Ingår i: Scandinavian Journal of Medicine and Science in Sports, ISSN 0905-7188, E-ISSN 1600-0838, Vol. 19, nr 6, s. 865-870Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

This study has examined exercise capacity and muscle morphology in patients with different severities of chronic obstructive pulmonary disease (COPD). Twenty-three patients and 12 healthy matched controls were recruited. Based on the severity of airflow obstruction, patients were divided into two subgroups. Exercise capacity was determined using a 6-min walk test. Muscle fiber composition, fiber area and number of satellite cells/muscle fiber were determined in muscle biopsies using immunohistochemistry. A progressive decline in exercise capacity was noted with ascending disease severity. Furthermore, a correlation between reduction in exercise capacity and changes in muscle fiber composition was observed in COPD. The group with severe and very severe COPD had a lower proportion of type I and a higher proportion of type IIa fibers compared with the other groups. In severe and very severe COPD, a reduction in fiber area of type IIa fibers was also seen. The number of satellite cells/muscle fiber did not differ between the groups. In conclusion, a decline in exercise capacity occurs already in mild and moderate COPD, indicating that the 6-min walk test is a reliable indicator of disease severity. Furthermore, changes in skeletal muscle morphology are associated with disease severity while muscle regenerative capacity is not altered.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell Publishing Inc., 2009
Nyckelord
Activities of Daily Living/*psychology, Adaptation; Psychological, Aged, Aged; 80 and over, Attitude to Health, Body Image, Dependency (Psychology), Female, Health Services Needs and Demand, Humans, Interpersonal Relations, Male, Middle Aged, Models; Psychological, Neoplasms/psychology, Nursing Methodology Research, Palliative Care/methods/*psychology, Power (Psychology), Quality of Life/psychology, Questionnaires, Respite Care/psychology, Self Concept, Social Support, Sweden
Nationell ämneskategori
Medicin och hälsovetenskap Fysiologi Lungmedicin och allergi Idrottsvetenskap
Forskningsämne
Fysiologi; Idrottsvetenskap
Identifikatorer
urn:nbn:se:oru:diva-3417 (URN)10.1111/j.1600-0838.2008.00858.x (DOI)000272132200014 ()18980606 (PubMedID)2-s2.0-70450260481 (Scopus ID)
Tillgänglig från: 2008-12-04 Skapad: 2008-12-04 Senast uppdaterad: 2018-01-13Bibliografiskt granskad
Wåhlin-Larsson, B., Ulfberg, J., Piehl-Aulin, K. & Kadi, F. (2009). The expression of vascular endothelial growth factor in skeletal muscle of patients with sleep disorders. Muscle and Nerve, 40(4), 556-561
Öppna denna publikation i ny flik eller fönster >>The expression of vascular endothelial growth factor in skeletal muscle of patients with sleep disorders
2009 (Engelska)Ingår i: Muscle and Nerve, ISSN 0148-639X, E-ISSN 1097-4598, Vol. 40, nr 4, s. 556-561Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

An increased capillary network has been observed in the skeletal muscle in patients with Restless Legs Syndrome (RLS) and Obstructive sleep apnea syndrome (OSAS). These changes could be due to upregulation of growth factors responsible for angiogenesis.

The aim of the study was to examine the occurrence and localization of VEGF and capillary proliferation in skeletal muscle of RLS (n=12), OSAS (n=12) and controls (n=11).

Double-immunofluorescence staining for capillaries (CD31) and VEGF, and proliferating cells (Ki-67), was carried out on biopsies taken from the tibialis anterior. The percentage of capillaries expressing VEGF (CD31,VEGF+)  was significantly higher in OSAS and RLS compared to controls. The percentage of proliferating capillaries (CD31,Ki-67+)  was significantly higher in OSAS compared with controls.

In conclusion our study shows the occurrence of proliferation of endothelial cells in skeletal muscle in RLS and OSAS, supporting an upregulation of VEGF located in capillaries probably due to local hypoxia.

Ort, förlag, år, upplaga, sidor
New York, NY: John Wiley & Sons, 2009
Nyckelord
VEGF, immunofluorescence, capillary, RLS, OSAS
Nationell ämneskategori
Medicin och hälsovetenskap Tvärvetenskapliga studier inom samhällsvetenskap Idrottsvetenskap
Forskningsämne
Idrottsvetenskap
Identifikatorer
urn:nbn:se:oru:diva-6213 (URN)10.1002/mus.21357 (DOI)000270794900006 ()
Tillgänglig från: 2009-04-08 Skapad: 2009-04-08 Senast uppdaterad: 2018-01-13Bibliografiskt granskad

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