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Fjaeraa, Christina
Alternative names
Publications (5 of 5) Show all publications
Fjæraa Alfredsson, C., Rendel, F., Liang, Q.-L., Sundström, B. E. & Nånberg, E. (2015). Altered sensitivity to ellagic acid in neuroblastoma cells undergoing differentiation with 12-0-tetradecanoylphorbol-13-acetate and all-trans retinoic acid. Biomedicine and Pharmacotherapy, 76, 39-45
Open this publication in new window or tab >>Altered sensitivity to ellagic acid in neuroblastoma cells undergoing differentiation with 12-0-tetradecanoylphorbol-13-acetate and all-trans retinoic acid
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2015 (English)In: Biomedicine and Pharmacotherapy, ISSN 0753-3322, E-ISSN 1950-6007, Vol. 76, p. 39-45Article in journal (Refereed) Published
Abstract [en]

Ellagic acid has previously been reported to induce reduced proliferation and activation of apoptosis in several tumor cell lines including our own previous data from non-differentiated human neuroblastoma SH-SY5Y cells. The aim of this study was now to investigate if in vitro differentiation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate or the vitamin A derivative all-trans retinoic acid altered the sensitivity to ellagic acid in SH-SY5Y cells. The methods used were cell counting and LDH-assay for evaluation of cell number and cell death, flow cytometric analysis of SubG(1)-and TUNEL-analysis for apoptosis and western blot for expression of apoptosis-associated proteins. In vitro differentiation was shown to reduce the sensitivity to ellagic acid with respect to cell detachment, loss of viability and activation of apoptosis. The protective effect was phenotype-specific and most prominent in all-trans retinoic acid-differentiated cultures. Differentiation-dependent up-regulation of Bcl-2 and integrin expression is introduced as possible protective mechanisms. The presented data also point to a positive correlation between proliferative activity and sensitivity to ellagic-acid-induced cell detachment. In conclusion, the presented data emphasize the need to consider degree of neuronal differentiation and phenotype of neuroblastoma cells when discussing a potential pharmaceutical application of ellagic acid in tumor treatment.

Place, publisher, year, edition, pages
Elsevier, 2015
Keywords
Ellagic acid, Cell adhesion, Apoptosis, Neuroblastoma, Differentiation
National Category
Basic Medicine Cell and Molecular Biology
Research subject
Biomedical Sciences
Identifiers
urn:nbn:se:oru:diva-79030 (URN)10.1016/j.biopha.2015.10.008 (DOI)000367541500007 ()26653548 (PubMedID)2-s2.0-84949206850 (Scopus ID)
Funder
The Royal Swedish Academy of Sciences
Note

This work was financially supported by The County Council of Värmland, The Swedish Childrens Cancer Society and The Royal Swedish Academy of Sciences.

Available from: 2013-10-24 Created: 2020-01-17 Last updated: 2020-01-27Bibliographically approved
Fjæraa Alfredsson, C., Ding, M., Liang, Q.-L., Sundström, B. & Nånberg, E. (2014). Ellagic acid induces a dose- and time-dependent depolarization of mitochondria and activation of caspase-9 and -3 in human neuroblastoma cells. Biomedicine and Pharmacotherapy, 68(1), 129-135
Open this publication in new window or tab >>Ellagic acid induces a dose- and time-dependent depolarization of mitochondria and activation of caspase-9 and -3 in human neuroblastoma cells
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2014 (English)In: Biomedicine and Pharmacotherapy, ISSN 0753-3322, E-ISSN 1950-6007, Vol. 68, no 1, p. 129-135Article in journal (Refereed) Published
Abstract [en]

The polyphenol ellagic acid is found in many natural food sources and has been proposed as a candidate compound for clinical applications due to its anti-oxidative capacity and as a potential anti-tumorigenic compound. The objective of the present study was to evaluate the sensitivity to and possible apoptosis mechanism induced by ellagic acid in neuronal tumor cells. As a model the human neuroblastoma SH-SY5Y cell line was used. The methods applied were bright field and phase contrast microscopy, XTT- and LDH-assays, western blot, and flow cytometric analysis of DNA degradation and mitochondrial membrane potential. Ellagic acid treatment was found to induce a reduction in cell number preceded by alterations of the mitochondrial membrane potential and activation of caspase-9 and -3, DNA-fragmentation and cell death by apoptosis. The apoptotic cell death studied was not due to anoikis since it was significant in the adherent fraction of the cells. We conclude that ellagic acid induces dose- and time-dependent apoptosis, at least partly by the mitochondrial pathway, in an embryonal neuronal tumor cell system. This finding is in agreement with previously reported data on adult carcinoma cells thus suggesting a more general effect of ellagic acid on tumor cells.

Place, publisher, year, edition, pages
Elsevier, 2014
Keywords
Ellagic acid, Caspase, Mitochondrial membrane potential
National Category
Medical and Health Sciences Pharmacology and Toxicology
Identifiers
urn:nbn:se:oru:diva-79029 (URN)10.1016/j.biopha.2013.08.010 (DOI)000332448400019 ()24051122 (PubMedID)2-s2.0-84895072497 (Scopus ID)
Note

This work was financially supported by The County Council of Värmland and The Swedish Childrens Cancer Society.

Available from: 2013-10-17 Created: 2020-01-17 Last updated: 2020-01-27Bibliographically approved
Karlsson, S., Nånberg, E., Fjaeraa, C. & Wijkander, J. (2010). Ellagic acid inhibits lipopolysaccharide-induced expression of enzymes involved in the synthesis of prostaglandin E2 in human monocytes. British Journal of Nutrition, 103(8), 1102-1109
Open this publication in new window or tab >>Ellagic acid inhibits lipopolysaccharide-induced expression of enzymes involved in the synthesis of prostaglandin E2 in human monocytes
2010 (English)In: British Journal of Nutrition, ISSN 0007-1145, E-ISSN 1475-2662, Vol. 103, no 8, p. 1102-1109Article in journal (Refereed) Published
Abstract [en]

Ellagic acid, a natural polyphenol found in certain fruits, nuts and vegetables, has in recent years been the subject of intense research within the fields of cancer and inflammation. Pain, fever and swelling, all typical symptoms of inflammation, are ascribed to elevated levels of PGE(2). In the present study, we have investigated the effects of ellagic acid on PGE(2) release and on prostaglandin-synthesising enzymes in human monocytes. Ellagic acid was found to inhibit Ca ionophore A23187-, phorbol myristate acetate- and opsonised zymosan-induced release of PGE(2) from monocytes pre-treated with the inflammatory agent lipopolysaccharide. Ellagic acid suppressed the lipopolysaccharide-induced increase in protein expression of cyclo-oxygenase-2 (COX-2), microsomal PGE synthase-1 (mPGEs-1) and cytosolic phospholipase A(2)alpha (cPLA(2)alpha), while it had no effect on the constitutively expressed COX-1 protein. Ellagic acid had no apparent inhibitory effect on these enzymes when the activities were determined in cell-free assays. We conclude that the inhibitory effect of ellagic acid on PGE(2) release from monocytes is due to a suppressed expression of COX-2, mPGEs-1 and cPLA(2)alpha, rather than a direct effect on the activities of these enzymes.

Place, publisher, year, edition, pages
Cambridge University Press, 2010
Keywords
Ellagic acid, Prostaglandin E2, Human monocytes
National Category
Basic Medicine Biochemistry and Molecular Biology Pharmacology and Toxicology
Research subject
Biology
Identifiers
urn:nbn:se:oru:diva-79033 (URN)10.1017/S0007114509992935 (DOI)000277248800004 ()19948080 (PubMedID)2-s2.0-77951633127 (Scopus ID)
Note

The present study was financially supported by The County Council of Värmland.

Available from: 2013-11-21 Created: 2020-01-17 Last updated: 2020-01-27Bibliographically approved
Fjaeraa, C. & Nånberg, E. (2009). Effect of ellagic acid on proliferation, cell adhesion and apoptosis in SH-SY5Y human neuroblastoma cells. Biomedicine and Pharmacotherapy, 63(4), 254-261
Open this publication in new window or tab >>Effect of ellagic acid on proliferation, cell adhesion and apoptosis in SH-SY5Y human neuroblastoma cells
2009 (English)In: Biomedicine and Pharmacotherapy, ISSN 0753-3322, E-ISSN 1950-6007, Vol. 63, no 4, p. 254-261Article in journal (Refereed) Published
Abstract [en]

Ellagic acid, a polyphenolic compound found in berries, fruits and nuts, has been shown to possess growth-inhibiting and apoptosis promoting activities in cancer cell lines in vitro. The objective of this study was to investigate the effect of ellagic acid in human neuroblastoma SH-SY5Y cells. In cultures of SH-SY5Y cells incubated with ellagic acid, time- and concentration-dependent inhibitory effects on cell number were demonstrated.Ellagic acid induced cell detachment, decreased cell viability and induced apoptosis as measured by DNA strand breaks. Ellagic acid-induced alterations in cell cycle were also observed. Simultaneous treatment with all-trans retinoic acid did not rescue the cells from ellagic acid effects. Furthermore, the results suggested that pre-treatment with all-trans retinoic acid to induce differentiation and cell cycle arrest did not rescue the cells from ellagic acid-induced cell death.

Place, publisher, year, edition, pages
Elsevier, 2009
Keywords
Ellagic acid, Apoptosis, Neuroblastoma
National Category
Biological Sciences Cell Biology
Research subject
Biomedical Sciences
Identifiers
urn:nbn:se:oru:diva-79028 (URN)10.1016/j.biopha.2008.07.093 (DOI)000266350500003 ()18848760 (PubMedID)2-s2.0-64749108144 (Scopus ID)
Note

This work was financially supported by The County Council of Värmland and The Swedish Children’s Cancer Society.

Available from: 2012-02-08 Created: 2020-01-17 Last updated: 2020-01-27Bibliographically approved
Fjæraa Alfredsson, C., Rendel, F., Sundström, B. E. & Nånberg, E.Proliferation in morphologically differentiated SH-SY5Y human neuroblastoma cells.
Open this publication in new window or tab >>Proliferation in morphologically differentiated SH-SY5Y human neuroblastoma cells
(English)Manuscript (preprint) (Other academic)
National Category
Medical and Health Sciences Cell and Molecular Biology
Research subject
Biomedical Sciences
Identifiers
urn:nbn:se:oru:diva-79031 (URN)
Available from: 2013-10-24 Created: 2020-01-17 Last updated: 2020-02-03Bibliographically approved
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