oru.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Khalaf, Hazem
Publications (10 of 46) Show all publications
Bengtsson, T., Selegård, R., Musa, A., Hultenby, K., Utterström, J., Sivlér, P., . . . Khalaf, H. (2020). Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics. Scientific Reports, 10(1), Article ID 3580.
Open this publication in new window or tab >>Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics
Show others...
2020 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 10, no 1, article id 3580Article in journal (Refereed) Published
Abstract [en]

The use of conventional antibiotics has substantial clinical efficacy, however these vital antimicrobial agents are becoming less effective due to the dramatic increase in antibiotic-resistant bacteria. Novel approaches to combat bacterial infections are urgently needed and bacteriocins represent a promising alternative. In this study, the activities of the two-peptide bacteriocin PLNC8 αβ were investigated against different Staphylococcus spp. The peptide sequences of PLNC8 α and β were modified, either through truncation or replacement of all L-amino acids with D-amino acids. Both L- and D-PLNC8 αβ caused rapid disruption of lipid membrane integrity and were effective against both susceptible and antibiotic resistant strains. The D-enantiomer was stable against proteolytic degradation by trypsin compared to the L-enantiomer. Of the truncated peptides, β1-22, β7-34 and β1-20 retained an inhibitory activity. The peptides diffused rapidly (2 min) through the bacterial cell wall and permeabilized the cell membrane, causing swelling with a disorganized peptidoglycan layer. Interestingly, sub-MIC concentrations of PLNC8 αβ substantially enhanced the effects of different antibiotics in an additive or synergistic manner. This study shows that PLNC8 αβ is active against Staphylococcus spp. and may be developed as adjuvant in combination therapy to potentiate the effects of antibiotics and reduce their overall use.

Place, publisher, year, edition, pages
Nature Publishing Group, 2020
National Category
Infectious Medicine
Identifiers
urn:nbn:se:oru:diva-80305 (URN)10.1038/s41598-020-60570-w (DOI)32107445 (PubMedID)2-s2.0-85081035544 (Scopus ID)
Available from: 2020-03-03 Created: 2020-03-03 Last updated: 2020-03-25Bibliographically approved
Selegård, R., Musa, A., Nyström, P., Aili, D., Bengtsson, T. & Khalaf, H. (2019). Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis. Future microbiology, 14(3), 195-206
Open this publication in new window or tab >>Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis
Show others...
2019 (English)In: Future microbiology, ISSN 1746-0913, Vol. 14, no 3, p. 195-206Article in journal (Refereed) Published
Abstract [en]

AIM: Bacteriocins are considered as promising alternatives to antibiotics against infections. In this study, the plantaricins (Pln) A, E, F, J and K were investigated for their antimicrobial activity against Staphylococcus epidermidis.

MATERIALS & METHODS: The effects on membrane integrity were studied using liposomes and viable bacteria, respectively.

RESULTS: We show that PlnEF and PlnJK caused rapid and significant lysis of S. epidermidis, and induced lysis of liposomes. The PlnEF and PlnJK displayed similar mechanisms by targeting and disrupting the bacterial cell membrane. Interestingly, Pln enhanced the effects of different antibiotics by 30- to 500-fold.

CONCLUSION: This study shows that Pln in combination with low concentrations of antibiotics is efficient against S. epidermidis and may be developed as potential treatment of infections.

Place, publisher, year, edition, pages
Future Medicine, 2019
Keywords
Pln, antibiotic, combination therapy, liposome, membrane lysis, plantaricin, synergy
National Category
Infectious Medicine Microbiology in the medical area
Identifiers
urn:nbn:se:oru:diva-71656 (URN)10.2217/fmb-2018-0285 (DOI)000460352400005 ()30648887 (PubMedID)2-s2.0-85062167749 (Scopus ID)
Funder
Magnus Bergvall Foundation, 2015-00823Knowledge Foundation, 20150244 20150086Swedish Research Council, 2016-04874
Available from: 2019-01-22 Created: 2019-01-22 Last updated: 2019-03-19Bibliographically approved
Fürsatz, M., Skog, M., Sivlér, P., Palm, E., Aronsson, C., Skallberg, A., . . . Aili, D. (2018). Functionalization of bacterial cellulose wound dressings with the antimicrobial peptide ε-poly-L-Lysine. Biomedical Materials, 13, Article ID 025014.
Open this publication in new window or tab >>Functionalization of bacterial cellulose wound dressings with the antimicrobial peptide ε-poly-L-Lysine
Show others...
2018 (English)In: Biomedical Materials, ISSN 1748-6041, E-ISSN 1748-605X, Vol. 13, article id 025014Article in journal (Refereed) Published
Abstract [en]

Wound dressings based on bacterial cellulose (BC) can form a soft and conformable protective layer that can stimulate wound healing while preventing bacteria from entering the wound. Bacteria already present in the wound can, however, thrive in the moist environment created by the BC dressing which can aggravate the healing process. Possibilities to render the BC antimicrobial without affecting the beneficial structural and mechanical properties of the material would hence be highly attractive. Here we present methods for functionalization of BC with ε-Poly-L-Lysine (ε-PLL), a non-toxic biopolymer with broad-spectrum antimicrobial activity. Low molecular weight ε-PLL was cross-linked in pristine BC membranes and to carboxymethyl cellulose (CMC) functionalized BC using carbodiimide chemistry. The functionalization of BC with ε-PLL inhibited growth of S. epidermidis on the membranes but did not affect the cytocompatibility to cultured human fibroblasts as compared to native BC. The functionalization had no significant effects on the nanofibrous structure and mechanical properties of the BC. The possibility to functionalize BC with ε-PLL is a promising, green and versatile approach to improve the performance of BC in wound care and other biomedical applications.

Place, publisher, year, edition, pages
Institute of Physics Publishing (IOPP), 2018
Keywords
bacterial cellulose; antimicrobial; carboxymethyl cellulose; epsilon-poly-L-Lysine
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Biomaterials Science Textile, Rubber and Polymeric Materials
Identifiers
urn:nbn:se:oru:diva-62415 (URN)10.1088/1748-605X/aa9486 (DOI)000423860700006 ()29047451 (PubMedID)
Funder
Carl Tryggers foundation Knowledge Foundation
Note

Funding Agencies:

Linkoping University  2009 00971 

Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linkoping University  2009 00971 

Available from: 2017-12-11 Created: 2017-12-11 Last updated: 2018-08-16Bibliographically approved
Jayaprakash, K., Demirel, I., Khalaf, H. & Bengtsson, T. (2018). Porphyromonas gingivalis-induced inflammatory responses in THP1 cells are altered by native and modified low-density lipoproteins in a strain-dependent manner. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 126(8), 667-677
Open this publication in new window or tab >>Porphyromonas gingivalis-induced inflammatory responses in THP1 cells are altered by native and modified low-density lipoproteins in a strain-dependent manner
2018 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 126, no 8, p. 667-677Article in journal (Refereed) Published
Abstract [en]

Strong epidemiological evidence supports an association between cardiovascular and periodontal disease and furthermore, the periodontopathogen Porphyromonas gingivalis has been identified in blood and from atheromatous plaques. Blood exposed to P.gingivalis shows an increased protein modification of low-density lipoprotein (LDL). In this study, we investigate the inflammatory responses of THP1 cells incubated with P.gingivalis and the effects of native or modified LDL on these responses. Reactive oxygen species (ROS) and IL-1 were observed in THP1 cells following infection with P.gingivalis ATCC33277 and W50. Caspase 1 activity was quantified in THP1 cells and correlated with IL-1 accumulation. Oxidized LDL (oxLDL) induced IL-1 release and CD36 expression on THP1 cells. Modified LDL co-stimulated with ATCC33277 exhibited regulatory effects on caspase 1 activity, IL-1 release and CD36 expression in THP1 cells, whereas W50 induced more modest responses in THP1 cells. In summary, we show that P.gingivalis is capable of inducing pro-inflammatory responses in THP1 cells, and native and modified LDL could alter these responses in a dose- and strain-dependent manner. Strain-dependent differences in THP1 cell responses could be due to the effect of P.gingivalis proteases, presence or absence of capsule and proteolytic transformation of native and modified LDL.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Inc., 2018
Keywords
Periodontitis, caspase 1, CD36, inflammation, IL-1beta
National Category
Immunology in the medical area Microbiology in the medical area
Identifiers
urn:nbn:se:oru:diva-68467 (URN)10.1111/apm.12860 (DOI)000440136000003 ()2-s2.0-85050727521 (Scopus ID)
Funder
Swedish Heart Lung FoundationKnowledge Foundation
Note

Funding Agency:

Foundation of Olle Engkvist

Available from: 2018-08-15 Created: 2018-08-15 Last updated: 2018-08-15Bibliographically approved
Bengtsson, T., Lönn, J., Khalaf, H. & Palm, E. (2018). The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria-induced proinflammatory responses in dermal fibroblasts. MicrobiologyOpen, 7(6), Article ID e606.
Open this publication in new window or tab >>The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria-induced proinflammatory responses in dermal fibroblasts
2018 (English)In: MicrobiologyOpen, ISSN 2045-8827, E-ISSN 2045-8827, Vol. 7, no 6, article id e606Article in journal (Refereed) Published
Abstract [en]

Antimicrobial resistance needs to be tackled from new angles, and antimicrobial peptides could be future candidates for combating bacterial infections. This study aims to investigate in vitro the bactericidal effects of the lantibiotic gallidermin on Staphylococcus epidermidis and Staphylococcus aureus, possible cytotoxic effects and its impact on host-microbe interactions. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of gallidermin were determined, and cytotoxicity and proinflammatory effects of gallidermin on fibroblasts, red blood cells (RBCs) and in whole blood were investigated. Both MIC and MBC for all four tested strains of S. epidermidis was 6.25 μg/ml. Both MIC and MBC for methicillin-sensitive S. aureus was 12.5 μg/ml and for methicillin-resistant S. aureus (MRSA) 1.56 μg/ml. Gallidermin displayed no cytotoxic effects on fibroblasts, only a high dose of gallidermin induced low levels of CXCL8 and interleukin-6. Gallidermin hemolyzed less than 1% of human RBCs, and did not induce reactive oxygen species production or cell aggregation in whole blood. In cell culture, gallidermin inhibited the cytotoxic effects of the bacteria and totally suppressed the bacteria-induced release of CXCL8 and interleukin-6 from fibroblasts. We demonstrate that gallidermin, expressing low cell cytotoxicity, is a promising candidate for treating bacterial infections caused by S. epidermidis and S. aureus, especially MRSA.

Place, publisher, year, edition, pages
John Wiley & Sons, 2018
Keywords
Streptococcus, antimicrobial resistance, bacteriocin, cytokines, fibroblasts, gallidermin
National Category
Immunology Microbiology in the medical area
Identifiers
urn:nbn:se:oru:diva-65822 (URN)10.1002/mbo3.606 (DOI)000453616500003 ()29536668 (PubMedID)2-s2.0-85043709207 (Scopus ID)
Funder
Knowledge Foundation, 20150244
Available from: 2018-03-15 Created: 2018-03-15 Last updated: 2019-01-08Bibliographically approved
Khalaf, H., Palm, E. & Bengtsson, T. (2017). Cellular Response Mechanisms in Porphyromonas gingivalis Infection. In: Pachiappan Arjunan (Ed.), Periodontitis: A Useful Reference (pp. 45-68). InTech
Open this publication in new window or tab >>Cellular Response Mechanisms in Porphyromonas gingivalis Infection
2017 (English)In: Periodontitis: A Useful Reference / [ed] Pachiappan Arjunan, InTech, 2017, p. 45-68Chapter in book (Refereed)
Abstract [en]

The pathogenicity of the periodontal biofilm is highly dependent on a few key species, of which Porphyromonas gingivalis is considered to be one of the most important pathogens. P. gingivalis expresses a broad range of virulence factors, of these cysteine proteases (gingipains) are of special importance both for the bacterial survival/proliferation and for the pathological outcome. Several cell types, for example, epithelial cells, endothelial cells, dendritic cells, osteoblasts, and fibroblasts, reside in the periodontium and are part of the innate host response, as well as platelets, neutrophils, lymphocytes, and monocytes/macrophages. These cells recognize and respond to P. gingivalis and its components through pattern recognition receptors (PRRs), for example, Toll-like receptors and protease-activated receptors. Ligation of PRRs induces downstream-signaling pathways modifying the activity of transcription factors that regulates the expression of genes linked to inflammation. This is followed by the release of inflammatory mediators, for example, cytokines and reactive oxygen species. Periodontal disease is today considered to play a significant role in various systemic conditions such as cardiovascular disease (CVD). The mechanisms by which P. gingivalis and its virulence factors interact with host immune cells and contribute to the pathogenesis of periodontitis and CVD are far from completely understood.

Abstract [en]

Periodontitis - A Useful Reference is a comprehensive book compiled by a team of experts with the objective of providing an overview of the basic pathology of "periodontitis" and its implication on oral health and general systemic health. Periodontitis has become a global health burden in recent days. It is noteworthy that oral health is being considered as the mirror of general health and the study of oral-systemic health connections has advanced among scientists, clinicians, and the public as well. We wish the array of chapters that highlights the importance and impact of periodontal health could be a useful guide for the community of public, students, and clinicians.

Place, publisher, year, edition, pages
InTech, 2017
Keywords
Host-microbe interaction, immune cells, pathogen recognition receptors, intracellular signaling, inflammatory responses, Porphyromonas gingivalis, gingipains, LPS, cardiovascular disease, treatment
National Category
Medical and Health Sciences Medical Bioscience
Research subject
Medicine; Biomedicine
Identifiers
urn:nbn:se:oru:diva-63388 (URN)978-953-51-3606-4 (ISBN)978-953-51-3605-7 (ISBN)
Available from: 2017-12-15 Created: 2017-12-15 Last updated: 2018-02-07Bibliographically approved
Bengtsson, T., Zhang, B., Selegård, R., Wiman, E., Aili, D. & Khalaf, H. (2017). Dual action of bacteriocin PLNC8 alpha beta through inhibition of Porphyromonas gingivalis infection and promotion of cell proliferation. Pathogens and Disease, 75(5), Article ID ftx064.
Open this publication in new window or tab >>Dual action of bacteriocin PLNC8 alpha beta through inhibition of Porphyromonas gingivalis infection and promotion of cell proliferation
Show others...
2017 (English)In: Pathogens and Disease, E-ISSN 2049-632X, Vol. 75, no 5, article id ftx064Article in journal (Refereed) Published
Abstract [en]

Periodontitis is a chronic inflammatory disease that is characterised by accumulation of pathogenic bacteria, including Porphyromonas gingivalis, in periodontal pockets. The lack of effective treatments has emphasised in an intense search for alternative methods to prevent bacterial colonisation and disease progression. Bacteriocins are bacterially produced antimicrobial peptides gaining increased consideration as alternatives to traditional antibiotics. We show rapid permeabilisation and aggregation of P. gingivalis by the two-peptide bacteriocin PLNC8 alpha beta. In a cell culture model, P. gingivalis was cytotoxic against gingival fibroblasts. The proteome profile of fibroblasts is severely affected by P. gingivalis, including induction of the ubiquitin-proteasome pathway. PLNC8 alpha beta enhanced the expression of growth factors and promoted cell proliferation, and suppressed proteins associated with apoptosis. PLNC8 alpha beta efficiently counteracted P. gingivalis-mediated cytotoxicity, increased expression of a large number of proteins and restored the levels of inflammatory mediators. In conclusion, we show that bacteriocin PLNC8 alpha beta displays dual effects by acting as a potent antimicrobial agent killing P. gingivalis and as a stimulatory factor promoting cell proliferation. We suggest preventive and therapeutical applications of PLNC8 alpha beta in periodontitis to supplement the host immune defence against P. gingivalis infection and support wound healing processes.

Place, publisher, year, edition, pages
Oxford University Press, 2017
Keywords
Porphyromonas gingivalis, periodontitis, cell proliferation, proteomics, bacteriocin, PLNC8
National Category
Immunology in the medical area Infectious Medicine Microbiology in the medical area
Identifiers
urn:nbn:se:oru:diva-59311 (URN)10.1093/femspd/ftx064 (DOI)000407245300014 ()2-s2.0-85028656048 (Scopus ID)
Funder
Knowledge Foundation, 20150244 20150086
Note

Funding Agency:

Foundation of Magnus Bergvall 2015-00823  

Available from: 2017-08-25 Created: 2017-08-25 Last updated: 2018-01-13Bibliographically approved
Jayaprakash, K., Demirel, I., Gunaltay, S., Khalaf, H. & Bengtsson, T. (2017). PKC, ERK/p38 MAP kinases and NF-B targeted signalling play a role in the expression and release of IL-1β  and CXCL8 in Porphyromonas gingivalis-infected THP1 cells. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 125(7), 623-633
Open this publication in new window or tab >>PKC, ERK/p38 MAP kinases and NF-B targeted signalling play a role in the expression and release of IL-1β  and CXCL8 in Porphyromonas gingivalis-infected THP1 cells
Show others...
2017 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 125, no 7, p. 623-633Article in journal (Refereed) Published
Abstract [en]

Porphyromonas gingivalis is a keystone pathogen in periodontitis and is gaining importance in cardiovascular pathogenesis. Protease-activated receptors (PARs), toll-like receptors (TLRs) and nucleotide-binding oligomerization domain (NOD) on monocytes recognize the structural components on P. gingivalis, inducing inflammatory intermediates. Here, we elucidate the modulation of PARs, TLRs, NODs, and the role of MAPK and NF-B in IL-1 and CXCL8 release. THP1 cells were stimulated with P. gingivalis wild-type W50 and its isogenic gingipain mutants: Rgp mutant E8 and Kgp mutant K1A. We observed modulation of PARs, TLRs, NOD, IL-1 and CXCL8 expression by P. gingivalis. Gingipains hydrolyse IL-1 and CXCL8, which is more evident for IL-1 accumulation at 24 h. Inhibition of PKC (protein kinase C), p38 and ERK (extracellular signal-regulated kinases) partially reduced P. gingivalis-induced IL-1 at 6 h, whereas PKC and ERK reduced CXCL8 at both 6 and 24 h. Following NF-B inhibition, P. gingivalis-induced IL-1 and CXCL8 were completely suppressed to basal levels. Overall, TLRs, PARs and NOD possibly act in synergy with PKC, MAPK ERK/p38 and NF-B in P. gingivalis-induced IL-1 and CXCL8 release from THP1 cells. These pro-inflammatory cytokines could affect leucocytes in circulation and exacerbate other vascular inflammatory conditions such as atherosclerosis.

Place, publisher, year, edition, pages
John Wiley & Sons, 2017
Keywords
THP1 cells, Porphyromonas gingivalis, chemokine ligand 8, interleukin-1, protease-activated receptors
National Category
Immunology in the medical area Microbiology in the medical area Cancer and Oncology
Identifiers
urn:nbn:se:oru:diva-58945 (URN)10.1111/apm.12701 (DOI)000403476400003 ()28493507 (PubMedID)2-s2.0-85019056141 (Scopus ID)
Funder
Swedish Heart Lung FoundationKnowledge Foundation
Note

Funding Agency:

Foundation of Olle Engkvist

Available from: 2017-08-18 Created: 2017-08-18 Last updated: 2018-01-13Bibliographically approved
Khalaf, H., Nakka, S. S., Sandén, C., Svärd, A., Hultenby, K., Scherbak, N., . . . Bengtsson, T. (2016). Antibacterial effects of Lactobacillus and bacteriocin PLNC8 αβ on the periodontal pathogen Porphyromonas gingivalis. BMC Microbiology, 16(1), Article ID 188.
Open this publication in new window or tab >>Antibacterial effects of Lactobacillus and bacteriocin PLNC8 αβ on the periodontal pathogen Porphyromonas gingivalis
Show others...
2016 (English)In: BMC Microbiology, ISSN 1471-2180, E-ISSN 1471-2180, Vol. 16, no 1, article id 188Article in journal (Refereed) Published
Abstract [en]

Background: The complications in healthcare systems associated with antibiotic-resistant microorganisms have resulted in an intense search for new effective antimicrobials. Attractive substances from which novel antibiotics may be developed are the bacteriocins. These naturally occurring peptides are generally considered to be safe and efficient at eliminating pathogenic bacteria. Among specific keystone pathogens in periodontitis, Porphyromonas gingivalis is considered to be the most important pathogen in the development and progression of chronic inflammatory disease. The aim of the present study was to investigate the antimicrobial effects of different Lactobacillus species and the two-peptide bacteriocin PLNC8 αβ on P. gingivalis.

Results: Growth inhibition of P. gingivalis was obtained by viable Lactobacillus and culture media from L. plantarum NC8 and 44048, but not L. brevis 30670. The two-peptide bacteriocin from L. plantarum NC8 (PLNC8 αβ) was found to be efficient against P. gingivalis through binding followed by permeabilization of the membranes, using Surface plasmon resonance analysis and DNA staining with Sytox Green. Liposomal systems were acquired to verify membrane permeabilization by PLNC8 αβ. The antimicrobial activity of PLNC8 αβ was found to be rapid (1 min) and visualized by TEM to cause cellular distortion through detachment of the outer membrane and bacterial lysis.

Conclusion: Soluble or immobilized PLNC8 αβ bacteriocins may be used to prevent P. gingivalis colonization and subsequent pathogenicity, and thus supplement the host immune system against invading pathogens associated with periodontitis.

Place, publisher, year, edition, pages
London, United Kingdom: BioMed Central, 2016
Keywords
Periodontitis, P. gingivalis, Lactobacillus, Bacteriocin, PLNC8
National Category
Microbiology
Identifiers
urn:nbn:se:oru:diva-51749 (URN)10.1186/s12866-016-0810-8 (DOI)000383422500001 ()27538539 (PubMedID)2-s2.0-84982308370 (Scopus ID)
Funder
Swedish Heart Lung FoundationKnowledge FoundationMagnus Bergvall Foundation
Note

Funding Agency:

Foundation of Olle Engkvist

Available from: 2016-08-23 Created: 2016-08-23 Last updated: 2017-11-28Bibliographically approved
Wickham, A., Vagin, M., Khalaf, H., Bertazzo, S., Hodder, P., Dånmark, S., . . . Aili, D. (2016). Electroactive biomimetic collagen-silver nanowire composite scaffolds. Nanoscale, 8(29), 14146-14155
Open this publication in new window or tab >>Electroactive biomimetic collagen-silver nanowire composite scaffolds
Show others...
2016 (English)In: Nanoscale, ISSN 2040-3364, E-ISSN 2040-3372, Vol. 8, no 29, p. 14146-14155Article in journal (Refereed) Published
Abstract [en]

Electroactive biomaterials are widely explored as bioelectrodes and as scaffolds for neural and cardiac regeneration. Most electrodes and conductive scaffolds for tissue regeneration are based on synthetic materials that have limited biocompatibility and often display large discrepancies in mechanical properties with the surrounding tissue causing problems during tissue integration and regeneration. This work shows the development of a biomimetic nanocomposite material prepared from self-assembled collagen fibrils and silver nanowires (AgNW). Despite consisting of mostly type I collagen fibrils, the homogeneously embedded AgNWs provide these materials with a charge storage capacity of about 2.3 mC cm(-2) and a charge injection capacity of 0.3 mC cm(-2), which is on par with bioelectrodes used in the clinic. The mechanical properties of the materials are similar to soft tissues with a dynamic elastic modulus within the lower kPa range. The nanocomposites also support proliferation of embryonic cardiomyocytes while inhibiting the growth of both Gram-negative Escherichia coli and Gram-positive Staphylococcus epidermidis. The developed collagen/AgNW composites thus represent a highly attractive bioelectrode and scaffold material for a wide range of biomedical applications.

Place, publisher, year, edition, pages
Cambridge: Royal Society of Chemistry, 2016
National Category
Chemical Sciences
Identifiers
urn:nbn:se:oru:diva-52701 (URN)10.1039/c6nr02027e (DOI)000381815000038 ()27385421 (PubMedID)2-s2.0-84979626544 (Scopus ID)
Funder
Swedish Foundation for Strategic Research
Note

Funding Agency:

Linköping University

Available from: 2016-09-30 Created: 2016-09-30 Last updated: 2020-01-29Bibliographically approved
Organisations

Search in DiVA

Show all publications