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Wiman, E., Zattarin, E., Aili, D., Bengtsson, T., Selegård, R. & Khalaf, H. (2023). Development of novel broad-spectrum antimicrobial lipopeptides derived from plantaricin NC8 β. Scientific Reports, 13(1), Article ID 4104.
Open this publication in new window or tab >>Development of novel broad-spectrum antimicrobial lipopeptides derived from plantaricin NC8 β
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2023 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 13, no 1, article id 4104Article in journal (Refereed) Published
Abstract [en]

Bacterial resistance towards antibiotics is a major global health issue. Very few novel antimicrobial agents and therapies have been made available for clinical use during the past decades, despite an increasing need. Antimicrobial peptides have been intensely studied, many of which have shown great promise in vitro. We have previously demonstrated that the bacteriocin Plantaricin NC8 αβ (PLNC8 αβ) from Lactobacillus plantarum effectively inhibits Staphylococcus spp., and shows little to no cytotoxicity towards human keratinocytes. However, due to its limitations in inhibiting gram-negative species, the aim of the present study was to identify novel antimicrobial peptidomimetic compounds with an enhanced spectrum of activity, derived from the β peptide of PLNC8 αβ. We have rationally designed and synthesized a small library of lipopeptides with significantly improved antimicrobial activity towards both gram-positive and gram-negative bacteria, including the ESKAPE pathogens. The lipopeptides consist of 16 amino acids with a terminal fatty acid chain and assemble into micelles that effectively inhibit and kill bacteria by permeabilizing their cell membranes. They demonstrate low hemolytic activity and liposome model systems further confirm selectivity for bacterial lipid membranes. The combination of lipopeptides with different antibiotics enhanced the effects in a synergistic or additive manner. Our data suggest that the novel lipopeptides are promising as future antimicrobial agents, however additional experiments using relevant animal models are necessary to further validate their in vivo efficacy.

Place, publisher, year, edition, pages
Springer Nature, 2023
National Category
Microbiology
Identifiers
urn:nbn:se:oru:diva-104967 (URN)10.1038/s41598-023-31185-8 (DOI)000988825800016 ()36914718 (PubMedID)2-s2.0-85150098922 (Scopus ID)
Funder
Swedish Foundation for Strategic Research, RMX18 0039Knowledge Foundation, 20180148
Available from: 2023-03-15 Created: 2023-03-15 Last updated: 2024-01-02Bibliographically approved
Eskilson, O., Zattarin, E., Berglund, L., Oksman, K., Hanna, K., Rakar, J., . . . Aili, D. (2023). Nanocellulose composite wound dressings for real-time pH wound monitoring. Materials today. Bio, 19, Article ID 100574.
Open this publication in new window or tab >>Nanocellulose composite wound dressings for real-time pH wound monitoring
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2023 (English)In: Materials today. Bio, E-ISSN 2590-0064, Vol. 19, article id 100574Article in journal (Refereed) Published
Abstract [en]

The skin is the largest organ of the human body. Wounds disrupt the functions of the skin and can have catastrophic consequences for an individual resulting in significant morbidity and mortality. Wound infections are common and can substantially delay healing and can result in non-healing wounds and sepsis. Early diagnosis and treatment of infection reduce risk of complications and support wound healing. Methods for monitoring of wound pH can facilitate early detection of infection. Here we show a novel strategy for integrating pH sensing capabilities in state-of-the-art hydrogel-based wound dressings fabricated from bacterial nanocellulose (BC). A high surface area material was developed by self-assembly of mesoporous silica nanoparticles (MSNs) in BC. By encapsulating a pH-responsive dye in the MSNs, wound dressings for continuous pH sensing with spatiotemporal resolution were developed. The pH responsive BC-based nanocomposites demonstrated excellent wound dressing properties, with respect to conformability, mechanical properties, and water vapor transmission rate. In addition to facilitating rapid colorimetric assessment of wound pH, this strategy for generating functional BC-MSN nanocomposites can be further be adapted for encapsulation and release of bioactive compounds for treatment of hard-to-heal wounds, enabling development of novel wound care materials.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Bacterial nanocellulose, Infection, Mesoporous silica nanoparticles, Wound dressing, pH sensor
National Category
Biomaterials Science
Identifiers
urn:nbn:se:oru:diva-104577 (URN)10.1016/j.mtbio.2023.100574 (DOI)000944392500001 ()36852226 (PubMedID)2-s2.0-85148095686 (Scopus ID)
Funder
Swedish Foundation for Strategic Research, FFL15-0026 RMX18-0039Vinnova, 2016-05156Knut and Alice Wallenberg Foundation, 2016.0231Swedish Research Council, 2021-04427
Note

Funding agencies:

Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linköping University 2009-00971

Swedish strategic research program Bio4Energy

Available from: 2023-03-01 Created: 2023-03-01 Last updated: 2024-01-02Bibliographically approved
Omer, A. A. M., Hinkula, J., Tran, P. T., Melik, W., Zattarin, E., Aili, D., . . . Khalaf, H. (2022). Plantaricin NC8 αβ rapidly and efficiently inhibits flaviviruses and SARS-CoV-2 by disrupting their envelopes. PLOS ONE, 17(11), Article ID e0278419.
Open this publication in new window or tab >>Plantaricin NC8 αβ rapidly and efficiently inhibits flaviviruses and SARS-CoV-2 by disrupting their envelopes
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2022 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 17, no 11, article id e0278419Article in journal (Refereed) Published
Abstract [en]

Potent broad-spectrum antiviral agents are urgently needed to combat existing and emerging viral infections. This is particularly important considering that vaccine development is a costly and time consuming process and that viruses constantly mutate and render the vaccine ineffective. Antimicrobial peptides (AMP), such as bacteriocins, are attractive candidates as antiviral agents against enveloped viruses. One of these bacteriocins is PLNC8 αβ, which consists of amphipathic peptides with positive net charges that display high affinity for negatively charged pathogen membrane structures, including phosphatidylserine rich lipid membranes of viral envelopes. Due to the morphological and physiological differences between viral envelopes and host cell plasma membranes, PLNC8 αβ is thought to have high safety profile by specifically targeting viral envelopes without effecting host cell membranes. In this study, we have tested the antiviral effects of PLNC8 αβ against the flaviviruses Langat and Kunjin, coronavirus SARS-CoV-2, influenza A virus (IAV), and human immunodeficiency virus-1 (HIV-1). The concentration of PLNC8 αβ that is required to eliminate all the infective virus particles is in the range of nanomolar (nM) to micromolar (μM), which is surprisingly efficient considering the high content of cholesterol (8–35%) in their lipid envelopes. We found that viruses replicating in the endoplasmic reticulum (ER)/Golgi complex, e.g. SARS-CoV-2 and flaviviruses, are considerably more susceptible to PLNC8 αβ, compared to viruses that acquire their lipid envelope from the plasma membrane, such as IAV and HIV-1. Development of novel broad-spectrum antiviral agents can significantly benefit human health by rapidly and efficiently eliminating infectious virions and thereby limit virus dissemination and spreading between individuals. PLNC8 αβ can potentially be developed into an effective and safe antiviral agent that targets the lipid compartments of viral envelopes of extracellular virions, more or less independent of virus antigenic mutations, which faces many antiviral drugs and vaccines.

Place, publisher, year, edition, pages
Public Library of Science, 2022
National Category
Microbiology in the medical area Cell and Molecular Biology Other Basic Medicine
Identifiers
urn:nbn:se:oru:diva-102724 (URN)10.1371/journal.pone.0278419 (DOI)000905496400010 ()36449554 (PubMedID)2-s2.0-85143180780 (Scopus ID)
Available from: 2022-12-14 Created: 2022-12-14 Last updated: 2023-02-02Bibliographically approved
Squinca, P., Berglund, L., Hanna, K., Rakar, J., Junker, J., Khalaf, H., . . . Oksman, K. (2021). Multifunctional Ginger Nanofiber Hydrogels with Tunable Absorption: The Potential for Advanced Wound Dressing Applications. Biomacromolecules, 22(8), 3202-3215
Open this publication in new window or tab >>Multifunctional Ginger Nanofiber Hydrogels with Tunable Absorption: The Potential for Advanced Wound Dressing Applications
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2021 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 22, no 8, p. 3202-3215Article in journal (Refereed) Published
Abstract [en]

In this study, ginger residue from juice production was evaluated as a raw material resource for preparation of nanofiber hydrogels with multifunctional properties for advanced wound dressing applications. Alkali treatment was applied to adjust the chemical composition of ginger fibers followed by TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical)-mediated oxidation prior to nanofiber isolation. The effect of alkali treatment on hydrogel properties assembled through vacuum filtration without addition of any chemical cross-linker was evaluated. An outstanding absorption ability of 6200% combined with excellent mechanical properties, tensile strength of 2.1 ± 0.2 MPa, elastic modulus of 15.3 ± 0.3 MPa, and elongation at break of 25.1%, was achieved without alkali treatment. Furthermore, the absorption capacity was tunable by applying alkali treatment at different concentrations and by adjusting the hydrogel grammage. Cytocompatibility evaluation of the hydrogels showed no significant effect on human fibroblast proliferation in vitro. Ginger essential oil was used to functionalize the hydrogels by providing antimicrobial activity, furthering their potential as a multifunctional wound dressing.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2021
National Category
Polymer Technologies
Identifiers
urn:nbn:se:oru:diva-93546 (URN)10.1021/acs.biomac.1c00215 (DOI)000685091300003 ()34254779 (PubMedID)2-s2.0-85111213327 (Scopus ID)
Funder
Swedish Foundation for Strategic Research , RMX18-0039The Kempe Foundations
Note

Funding Agencies:

Bio4Energy  

KAW 2018.0451

WWSC 

Coordenacao de Aperfeicamento de Pessoal de Nivel Superior, Brasil (CAPES) 001

Available from: 2021-08-10 Created: 2021-08-10 Last updated: 2024-01-02Bibliographically approved
Musa, A., Wiman, E., Selegård, R., Aili, D., Bengtsson, T. & Khalaf, H. (2021). Plantaricin NC8 αβ prevents Staphylococcus aureus-mediated cytotoxicity and inflammatory responses of human keratinocytes. Scientific Reports, 11(1), Article ID 12514.
Open this publication in new window or tab >>Plantaricin NC8 αβ prevents Staphylococcus aureus-mediated cytotoxicity and inflammatory responses of human keratinocytes
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2021 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 11, no 1, article id 12514Article in journal (Refereed) Published
Abstract [en]

Multidrug resistance bacteria constitue an increasing global health problem and the development of novel therapeutic strategies to face this challenge is urgent. Antimicrobial peptides have been proven as potent agents against pathogenic bacteria shown by promising in vitro results. The aim of this study was to characterize the antimicrobial effects of PLNC8 αβ on cell signaling pathways and inflammatory responses of human keratinocytes infected with S. aureus. PLNC8 αβ did not affect the viability of human keratinocytes but upregulated several cytokines (IL-1β, IL-6, CXCL8), MMPs (MMP1, MMP2, MMP9, MMP10) and growth factors (VEGF and PDGF-AA), which are essential in cell regeneration. S. aureus induced the expression of several inflammatory mediators at the gene and protein level and PLNC8 αβ was able to significantly suppress these effects. Intracellular signaling events involved primarily c-Jun via JNK, c-Fos and NFκB, suggesting their essential role in the initiation of inflammatory responses in human keratinocytes. PLNC8 αβ was shown to modulate early keratinocyte responses, without affecting their viability. The peptides have high selectivity towards S. aureus and were efficient at eliminating the bacteria and counteracting their inflammatory and cytotoxic effects, alone and in combination with low concentrations of gentamicin. We propose that PLNC8 αβ may be developed to combat infections caused by Staphylococcus spp.

Place, publisher, year, edition, pages
Nature Publishing Group, 2021
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:oru:diva-92410 (URN)10.1038/s41598-021-91682-6 (DOI)000664657500009 ()34131160 (PubMedID)2-s2.0-85108146841 (Scopus ID)
Funder
Knowledge Foundation, 20180148Swedish Foundation for Strategic Research , RMX18-0039
Available from: 2021-06-17 Created: 2021-06-17 Last updated: 2024-01-02Bibliographically approved
Davies, J. R., Kad, T., Neilands, J., Kinnby, B., Prgomet, Z., Bengtsson, T., . . . Svensäter, G. (2021). Polymicrobial synergy stimulates Porphyromonas gingivalis survival and gingipain expression in a multi-species subgingival community. BMC Oral Health, 21(1), Article ID 639.
Open this publication in new window or tab >>Polymicrobial synergy stimulates Porphyromonas gingivalis survival and gingipain expression in a multi-species subgingival community
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2021 (English)In: BMC Oral Health, ISSN 1472-6831, E-ISSN 1472-6831, Vol. 21, no 1, article id 639Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Dysbiosis in subgingival microbial communities, resulting from increased inflammatory transudate from the gingival tissues, is an important factor in initiation and development of periodontitis. Dysbiotic communities are characterized by increased numbers of bacteria that exploit the serum-like transudate for nutrients, giving rise to a proteolytic community phenotype. Here we investigate the contribution of interactions between members of a sub-gingival community to survival and development of virulence in a serum environment-modelling that in the subgingival pocket.

METHODS: Growth and proteolytic activity of three Porphyromonas gingivalis strains in nutrient broth or a serum environment were assessed using A600 and a fluorescent protease substrate, respectively. Adherence of P. gingivalis strains to serum-coated surfaces was studied with confocal microscopy and 2D-gel electrophoresis of bacterial supernatants used to investigate extracellular proteins. A model multi-species sub-gingival community containing Fusobacterium nucleatum, Streptococcus constellatus, Parvimonas micra with wild type or isogenic mutants of P. gingivalis was then created and growth and proteolytic activity in serum assessed as above. Community composition over time was monitored using culture techniques and qPCR.

RESULTS: The P. gingivalis strains showed different growth rates in nutrient broth related to the level of proteolytic activity (largely gingipains) in the cultures. Despite being able to adhere to serum-coated surfaces, none of the strains was able to grow alone in a serum environment. Together in the subgingival consortium however, all the included species were able to grow in the serum environment and the community adopted a proteolytic phenotype. Inclusion of P. gingivalis strains lacking gingipains in the consortium revealed that community growth was facilitated by Rgp gingipain from P. gingivalis.

CONCLUSIONS: In the multi-species consortium, growth was facilitated by the wild-type and Rgp-expressing strains of P. gingivalis, suggesting that Rgp is involved in delivery of nutrients to the whole community through degradation of complex protein substrates in serum. Whereas they are constitutively expressed by P. gingivalis in nutrient broth, gingipain expression in the model periodontal pocket environment (serum) appeared to be orchestrated through signaling to P. gingivalis from other members of the community, a phenomenon which then promoted growth of the whole community.

Place, publisher, year, edition, pages
BioMed Central, 2021
Keywords
Dysbiosis, Microbial community, Periodontitis, Proteolytic activity, Virulence
National Category
Dentistry
Identifiers
urn:nbn:se:oru:diva-96064 (URN)10.1186/s12903-021-01971-9 (DOI)000730556300002 ()34911531 (PubMedID)2-s2.0-85121339809 (Scopus ID)
Funder
Swedish Research CouncilKnowledge Foundation
Note

Funding agencies:

Malmö University

Foresight Research Programme at Malmö University

Odontologisk Forskning Region Skåne

Available from: 2021-12-17 Created: 2021-12-17 Last updated: 2024-01-02Bibliographically approved
Bengtsson, T., Selegård, R., Musa, A., Hultenby, K., Utterström, J., Sivlér, P., . . . Khalaf, H. (2020). Author Correction: Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics. Scientific Reports, 10(1), Article ID 16027.
Open this publication in new window or tab >>Author Correction: Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics
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2020 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 10, no 1, article id 16027Article in journal (Refereed) Published
Abstract [en]

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Place, publisher, year, edition, pages
Nature Publishing Group, 2020
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:oru:diva-86049 (URN)10.1038/s41598-020-72918-3 (DOI)000615373300001 ()32973307 (PubMedID)2-s2.0-85091429718 (Scopus ID)
Note

Erratum for Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics. Bengtsson T, Selegård R, Musa A, Hultenby K, Utterström J, Sivlér P, Skog M, Nayeri F, Hellmark B, Söderquist B, Aili D, Khalaf H. Sci Rep. 2020 Feb 27;10(1):3580. doi: 10.1038/s41598-020-60570-w.

Available from: 2020-09-29 Created: 2020-09-29 Last updated: 2024-01-02Bibliographically approved
Bengtsson, T., Selegård, R., Musa, A., Hultenby, K., Utterström, J., Sivlér, P., . . . Khalaf, H. (2020). Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics. Scientific Reports, 10(1), Article ID 3580.
Open this publication in new window or tab >>Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics
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2020 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 10, no 1, article id 3580Article in journal (Refereed) Published
Abstract [en]

The use of conventional antibiotics has substantial clinical efficacy, however these vital antimicrobial agents are becoming less effective due to the dramatic increase in antibiotic-resistant bacteria. Novel approaches to combat bacterial infections are urgently needed and bacteriocins represent a promising alternative. In this study, the activities of the two-peptide bacteriocin PLNC8 αβ were investigated against different Staphylococcus spp. The peptide sequences of PLNC8 α and β were modified, either through truncation or replacement of all L-amino acids with D-amino acids. Both L- and D-PLNC8 αβ caused rapid disruption of lipid membrane integrity and were effective against both susceptible and antibiotic resistant strains. The D-enantiomer was stable against proteolytic degradation by trypsin compared to the L-enantiomer. Of the truncated peptides, β1-22, β7-34 and β1-20 retained an inhibitory activity. The peptides diffused rapidly (2 min) through the bacterial cell wall and permeabilized the cell membrane, causing swelling with a disorganized peptidoglycan layer. Interestingly, sub-MIC concentrations of PLNC8 αβ substantially enhanced the effects of different antibiotics in an additive or synergistic manner. This study shows that PLNC8 αβ is active against Staphylococcus spp. and may be developed as adjuvant in combination therapy to potentiate the effects of antibiotics and reduce their overall use.

Place, publisher, year, edition, pages
Nature Publishing Group, 2020
National Category
Infectious Medicine
Identifiers
urn:nbn:se:oru:diva-80305 (URN)10.1038/s41598-020-60570-w (DOI)000560075900001 ()32107445 (PubMedID)2-s2.0-85081035544 (Scopus ID)
Funder
Magnus Bergvall Foundation, 2015-00823Knowledge Foundation, 20150244 20150086Swedish Research Council, 2016-04874 2017-04475Swedish Cancer Society, 17 0532Swedish Foundation for Strategic Research , RMX18-0039
Note

Funding Agencies:

Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linköping University  2009-00971

Örebro University

Erratum in Author Correction: Plantaricin NC8 αβ exerts potent antimicrobial activity against Staphylococcus spp. and enhances the effects of antibiotics. Bengtsson T, Selegård R, Musa A, Hultenby K, Utterström J, Sivlér P, Skog M, Nayeri F, Hellmark B, Söderquist B, Aili D, Khalaf H. Sci Rep. 2020 Sep 24;10(1):16027. doi: 10.1038/s41598-020-72918-3.

Available from: 2020-03-03 Created: 2020-03-03 Last updated: 2024-01-02Bibliographically approved
Eskilson, O., Lindström, S. B., Sepulveda, B., Shahjamali, M. M., Güell-Grau, P., Sivlér, P., . . . Aili, D. (2020). Self-Assembly of Mechanoplasmonic Bacterial Cellulose-Metal Nanoparticle Composites. Advanced Functional Materials, 30(40), Article ID 2004766.
Open this publication in new window or tab >>Self-Assembly of Mechanoplasmonic Bacterial Cellulose-Metal Nanoparticle Composites
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2020 (English)In: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 30, no 40, article id 2004766Article in journal (Refereed) Published
Abstract [en]

Nanocomposites of metal nanoparticles (NPs) and bacterial nanocellulose (BC) enable fabrication of soft and biocompatible materials for optical, catalytic, electronic, and biomedical applications. Current BC-NP nanocomposites are typically prepared by in situ synthesis of the NPs or electrostatic adsorption of surface functionalized NPs, which limits possibilities to control and tune NP size, shape, concentration, and surface chemistry and influences the properties and performance of the materials. Here a self-assembly strategy is described for fabrication of complex and well-defined BC-NP composites using colloidal gold and silver NPs of different sizes, shapes, and concentrations. The self-assembly process results in nanocomposites with distinct biophysical and optical properties. In addition to antibacterial materials and materials with excellent senor performance, materials with unique mechanoplasmonic properties are developed. The homogenous incorporation of plasmonic gold NPs in the BC enables extensive modulation of the optical properties by mechanical stimuli. Compression gives rise to near-field coupling between adsorbed NPs, resulting in tunable spectral variations and enhanced broadband absorption that amplify both nonlinear optical and thermoplasmonic effects and enables novel biosensing strategies.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2020
Keywords
antimicrobials, bacterial cellulose, gold nanoparticles, nanocomposite, sensors
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:oru:diva-85180 (URN)10.1002/adfm.202004766 (DOI)000557380700001 ()2-s2.0-85089155627 (Scopus ID)
Funder
Swedish Foundation for Strategic Research , FFL15-0026 RMX18-0039Vinnova, 2016-05156Knut and Alice Wallenberg Foundation, KAW 2016.0231Swedish Research Council, 2017-05178 2015-05002
Note

Funding Agencies:

Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linköping University  2009-00971

Spanish Ministerio de Ciencia, Innovacion y Universidades (MICINN)  MAT2016-77391-R

Severo Ochoa Centres of Excellence programme - Spanish Research Agency (AEI)  SEV-2017-0706

Available from: 2020-08-31 Created: 2020-08-31 Last updated: 2024-01-02Bibliographically approved
Selegård, R., Musa, A., Nyström, P., Aili, D., Bengtsson, T. & Khalaf, H. (2019). Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis. Future Microbiology, 14(3), 195-206
Open this publication in new window or tab >>Plantaricins markedly enhance the effects of traditional antibiotics against Staphylococcus epidermidis
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2019 (English)In: Future Microbiology, ISSN 1746-0913, E-ISSN 1746-0921, Vol. 14, no 3, p. 195-206Article in journal (Refereed) Published
Abstract [en]

AIM: Bacteriocins are considered as promising alternatives to antibiotics against infections. In this study, the plantaricins (Pln) A, E, F, J and K were investigated for their antimicrobial activity against Staphylococcus epidermidis.

MATERIALS & METHODS: The effects on membrane integrity were studied using liposomes and viable bacteria, respectively.

RESULTS: We show that PlnEF and PlnJK caused rapid and significant lysis of S. epidermidis, and induced lysis of liposomes. The PlnEF and PlnJK displayed similar mechanisms by targeting and disrupting the bacterial cell membrane. Interestingly, Pln enhanced the effects of different antibiotics by 30- to 500-fold.

CONCLUSION: This study shows that Pln in combination with low concentrations of antibiotics is efficient against S. epidermidis and may be developed as potential treatment of infections.

Place, publisher, year, edition, pages
Future Medicine, 2019
Keywords
Pln, antibiotic, combination therapy, liposome, membrane lysis, plantaricin, synergy
National Category
Infectious Medicine Microbiology in the medical area
Identifiers
urn:nbn:se:oru:diva-71656 (URN)10.2217/fmb-2018-0285 (DOI)000460352400005 ()30648887 (PubMedID)2-s2.0-85062167749 (Scopus ID)
Funder
Magnus Bergvall Foundation, 2015-00823Knowledge Foundation, 20150244 20150086Swedish Research Council, 2016-04874
Available from: 2019-01-22 Created: 2019-01-22 Last updated: 2024-01-02Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-3373-7864

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