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Geng, D., Musse, A. A., Wigh, V., Carlsson, C., Engwall, M., Oresic, M., . . . Hyötyläinen, T. (2019). Effect of perfluorooctanesulfonic acid (PFOS) on the liver lipid metabolism of the developing chicken embryo. Ecotoxicology and Environmental Safety, 170, 691-698
Open this publication in new window or tab >>Effect of perfluorooctanesulfonic acid (PFOS) on the liver lipid metabolism of the developing chicken embryo
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2019 (English)In: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 170, p. 691-698Article in journal (Refereed) Published
Abstract [en]

Perfluorooctanesulfonate (PFOS) is a well-known contaminant in the environment and it has shown to disrupt multiple biological pathways, particularly those related with lipid metabolism. In this study, we have studied the impact of in ovo exposure to PFOS on lipid metabolism in livers in developing chicken embryos using lipidomics for detailed characterization of the liver lipidome. We used an avian model (Gallus gallus domesticus) for in ovo treatment at two levels of PFOS. The lipid profile of the liver of the embryo was investigated by ultra-high performance liquid chromatography combined with quadrupole-time-of-flight mass spectrometry and by gas chromatography mass spectrometry. Over 170 lipids were identified, covering phospholipids, ceramides, di- and triacylglycerols, cholesterol esters and fatty acid composition of the lipids. The PFOS exposure caused dose dependent changes in the lipid levels, which included upregulation of specific phospholipids associated with the phosphatidylethanolamine N-methyltransferase (PEMT) pathway, triacylglycerols with low carbon number and double bond count as well as of lipotoxic ceramides and diacylglycerols. Our data suggest that at lower levels of exposure, mitochondrial fatty acid β-oxidation is suppressed while the peroxisomal fatty acid β -oxidation is increased. At higher doses, however, both β -oxidation pathways are upregulated.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Avian model, Lipidomics, Liver metabolism, Mass spectrometry, Perfluorooctanesulfonate
National Category
Pharmaceutical Sciences
Identifiers
urn:nbn:se:oru:diva-71192 (URN)10.1016/j.ecoenv.2018.12.040 (DOI)000456890700083 ()30580163 (PubMedID)2-s2.0-85058940877 (Scopus ID)
Funder
Swedish Research Council, 2016-05176Swedish Research Council FormasKnowledge Foundation
Available from: 2019-01-08 Created: 2019-01-08 Last updated: 2019-03-04Bibliographically approved
Geng, D., Kukucka, P. & Ericson Jogsten, I. (2017). Analysis of brominated flame retardants and their derivatives by atmospheric pressure chemical ionization using gas chromatography coupled to tandem quadrupole mass spectrometry. Talanta: The International Journal of Pure and Applied Analytical Chemistry, 162, 618-624
Open this publication in new window or tab >>Analysis of brominated flame retardants and their derivatives by atmospheric pressure chemical ionization using gas chromatography coupled to tandem quadrupole mass spectrometry
2017 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 162, p. 618-624Article in journal (Refereed) Published
Abstract [en]

A validated method using an atmospheric pressure chemical ionization source for coupling gas chromatography (GC-APCI) to tandem quadrupole mass spectrometry (MS/MS) for the determination of brominated flame retardants (BFRs) is presented. Polybrominated diphenyl ethers (PBDEs), their methoxylated derivatives (MeO-PBDEs) and other emerging BFRs were included in this study. The method showed good linearity and repeatability. The relative standard deviation (RSD) of the relative response factors (RRFs) of all compounds was less than 16%. Repeatability for BFRs was tested on one or two concentration levels of calibration standardswith RSDs for RRFs below 16%. The lowest calibration standards (0.075 –0.1 pg/μL for emerging BFRs, BDE 209 and MeO-PBDEs mixtures, 0.625 –6.25 pg/μL for Br1-9 PBDEs mixtures) were used as instrument detection limits (IDL). The method was applied on biotic samples, including fish, osprey, and seal. In general, BDE209 and decabromodiphenyl ethane (DBDPE) were detected in 50% of the seal samples. A 100% detection rate was achieved for 6-MeO-BDE47 in all the samples (72 –580 pg/g ww in osprey samples, 24 000 –96 000 pg/g ww in seal samples and 78–99 pg/g ww in fish samples). AllBr3-6PBDEs (BDE28, 47, 99, 100, 153, 154) were detected in all the samples (ranging from 12 to 20 000 pg/g ww), while BDE183 was detected in 60% of the osprey eggs, 20% of the seal samples and below MDL in all fish samples. The results presented indicate the capability of the GC-APCI-MS/MS system for the detection and quantification of BFRs.

Place, publisher, year, edition, pages
Amsterdam, Netherlands: Elsevier, 2017
Keywords
BFR, PBDE, MeO-PBDE, gas chromatography-atmospheric pressure chemical ionization (GC-APCI), tandem quadrupole mass spectrometry (MS/MS)
National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-53049 (URN)10.1016/j.talanta.2016.10.060 (DOI)000389088700083 ()27837880 (PubMedID)2-s2.0-84994381585 (Scopus ID)
Note

Funding Agencies:

China Scholarship Council 201206400003

Czech Ministry of Education, Youth and Sports LO1214  LM2015051

Ingrid Ericson Jogsten 20140057

Available from: 2016-10-19 Created: 2016-10-19 Last updated: 2017-11-29Bibliographically approved
Geng, D., Ericson Jogsten, I., Dunstan, J., Hagberg, J., Wang, T., Ruzzin, J., . . . van Bavel, B. (2016). Gas chromatography/atmospheric pressure chemical ionization/mass spectrometry for the analysis of organochlorine pesticides and polychlorinated biphenyls in human serum. Journal of Chromatography A, 1453, 88-98
Open this publication in new window or tab >>Gas chromatography/atmospheric pressure chemical ionization/mass spectrometry for the analysis of organochlorine pesticides and polychlorinated biphenyls in human serum
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2016 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1453, p. 88-98Article in journal (Refereed) Published
Abstract [en]

A method using a novel atmospheric pressure chemical ionization source for coupling gas chromatography (GC/APCI) to triple quadrupole mass spectrometry (MS/MS) for the determination of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) regulated by the Stockholm Convention is presented. One microliter injection of a six-point calibration curve of native PCBs and OCPs, ranging from 0.04 to 300 pg/μL, was performed. The relative standard deviation (RSD) of the relative response factors (RRFs) was less than 15% with a coefficient of determination (r2) >0.995. Meanwhile, two calibration solutions (CS), CS 2 (0.4 pg/μL) and CS 3 (4 pg/μL) were analyzed to study the repeatability calculated for both area and RRFs. The RSD for RRF ranged from 3.1 to 16% and 3.6 to 5.5% for CS 2 and CS 3, respectively. The limits of detection (LOD) determined by peak-to-peak signal-to-noise ratio (S/N) of 3 were compared between the GC/APCI/MS/MS and a GC coupled to high resolution mass spectrometry (GC/HRMS) system. GC/APCI/MS/MS resulted in lower LOD for most of the compounds, except for PCB#74, cis-chlordane and trans-chlordane. GC/APCI/MS/MS and GC/HRMS were also compared by performing analysis on 75 human serum samples together with eight QA/QC serum samples. The comparison between GC/APCI/MS/MS system and GC/HRMS system for 16 of the targeted compounds was carried out. No statistically significant difference was discovered. Due to increased sensitivity and user friendly operation under atmospheric pressure, GC/APCI/MS/MS is a powerful alternative technique that can easily meet the specification of GC/HRMS.

Place, publisher, year, edition, pages
Amsterdam, Netherlands: Elsevier, 2016
Keywords
PCBs, OCPs, atmospheric pressure chemical ionization (APCI), triple quadrupole mass spectrometry (MS/MS), high resolution mass spectrometry (GC/HRMS), human serum
National Category
Chemical Sciences
Research subject
Analytical Chemistry; Environmental Chemistry; Enviromental Science
Identifiers
urn:nbn:se:oru:diva-50854 (URN)10.1016/j.chroma.2016.05.030 (DOI)000378182800010 ()27236485 (PubMedID)2-s2.0-84969722268 (Scopus ID)
Note

Funding Agency:

China Scholarship Council (CSC) under the Grant CSC 201206400003

Available from: 2016-06-15 Created: 2016-06-14 Last updated: 2018-09-12Bibliographically approved
Geng, D. (2016). Gas chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry methods for the determination of environmental contaminants. (Doctoral dissertation). Örebro: Örebro university
Open this publication in new window or tab >>Gas chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry methods for the determination of environmental contaminants
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The recent developments and improvements of instrumental methods for the analyses of the environmental contaminants, especially the persistent organic pollutants (POPs), have made it possible to detect and quantify these at very low concentrations in environmental and biotic matrices.

The main objective of this thesis is to demonstrate the capability of the atmospheric pressure chemical ionization technique (APCI), using gas chromatography coupled to tandem mass spectrometry for the determination of a wide range of environmental contaminants, including the POPs regulated by Stockholm Convention, such as polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), but also the derivates of PBDEs and novel brominated flame retardants (NBFRs).

The APCI was operated in charge transfer condition, preferably producing molecular ions. Multiple reaction monitoring (MRM) experiments were optimized by adjusting cone voltage, collision energy and dwell time. Optimization of source parameters, such as gas flows and temperatures was also performed. Low concentration standards were analyzed, achieving a visible chromatographic peak for 2 fg 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) demonstrating the excellent sensitivity of the system. Adequate linearity and repeatability were observed for all the studied compounds. The performance of APCI methods was validated against the conventional methods using gas chromatography coupled to high resolution mass spectrometry for chlorinated compounds in a wide range of matrices including environmental, air, human and food matrices.

The GC-APCI-MS/MS method was successfully applied to a set of 75 human serum samples to study the circulating levels of POPs in epidemiologic studies. Moreover the method was utilized to establish temporal trends of POPs in osprey eggs samples collected during the past five decades.

Place, publisher, year, edition, pages
Örebro: Örebro university, 2016. p. 83
Series
Örebro Studies in Chemistry, ISSN 1651-4270 ; 17
Keywords
PCDD/Fs, PCBs, OCPs, PBDEs, NBFRs, APCI, MS/MS, HRMS, human serum, osprey egg
National Category
Chemical Sciences Other Chemistry Topics
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-51727 (URN)978-91-7668-157-4 (ISBN)
Public defence
2016-09-30, Örebro universitet, Musikhögskolan, Hörsalen, Fakultetsgatan 1, Örebro, 10:15 (English)
Opponent
Supervisors
Available from: 2016-08-22 Created: 2016-08-22 Last updated: 2017-10-17Bibliographically approved
Ericson Jogsten, I., Wang, T., Geng, D. & Roos, A. (2016). Temporal trends of persistent organochlorine and bromine compounds in ringed seals from the Baltic Sea (Phoca hispida baltica) from year 1974 to 2015. Paper presented at 36th International Symposium on Halogenated Persistent Organic Pollutants, Dioxin 2016, Florence, Italy, August 28 - September 2, 2016. Organohalogen Compounds, 78
Open this publication in new window or tab >>Temporal trends of persistent organochlorine and bromine compounds in ringed seals from the Baltic Sea (Phoca hispida baltica) from year 1974 to 2015
2016 (English)In: Organohalogen Compounds, ISSN 1026-4892, Vol. 78Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Eco-Informa Press, 2016
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:oru:diva-65626 (URN)
Conference
36th International Symposium on Halogenated Persistent Organic Pollutants, Dioxin 2016, Florence, Italy, August 28 - September 2, 2016
Available from: 2018-03-09 Created: 2018-03-09 Last updated: 2018-04-05Bibliographically approved
Geng, D., Kukucka, P., van Bavel, B., Ericson Jogsten, I. & Roos, A. (2016). Temporal Trends of Polychlorinated Biphenyls, Organochlorine Pesticides and Polybrominated Diphenyl Ethers in Osprey Eggs in Sweden over the Years 1966 – 2013. Paper presented at 36th International Symposium on Halogenated Persistent Organic Pollutants, Dioxin 2016, Florence, Italy, August 28 - September 2, 2016. Organohalogen Compounds, 78
Open this publication in new window or tab >>Temporal Trends of Polychlorinated Biphenyls, Organochlorine Pesticides and Polybrominated Diphenyl Ethers in Osprey Eggs in Sweden over the Years 1966 – 2013
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2016 (English)In: Organohalogen Compounds, ISSN 1026-4892, Vol. 78Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Eco-Informa Press, 2016
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:oru:diva-65669 (URN)
Conference
36th International Symposium on Halogenated Persistent Organic Pollutants, Dioxin 2016, Florence, Italy, August 28 - September 2, 2016
Available from: 2018-03-12 Created: 2018-03-12 Last updated: 2018-04-05Bibliographically approved
van Bavel, B., Geng, D., Cherta, L., Nacher-Mestre, J., Portoles, T., Abalos, M., . . . Hernandez, F. (2015). Atmospheric-Pressure Chemical Ionization Tandem Mass Spectrometry (APGC/MS/MS) an Alternative to High-Resolution Mass Spectrometry (HRGC/HRMS) for the Determination of Dioxins. Analytical Chemistry, 87(17), 9047-9053
Open this publication in new window or tab >>Atmospheric-Pressure Chemical Ionization Tandem Mass Spectrometry (APGC/MS/MS) an Alternative to High-Resolution Mass Spectrometry (HRGC/HRMS) for the Determination of Dioxins
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2015 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 87, no 17, p. 9047-9053Article in journal (Refereed) Published
Abstract [en]

The use of a new atmospheric-pressure chemical ionization source for gas chromatography (APGC) coupled with a tandem quadrupole mass spectrometry (MS/MS) system, as an alternative to high-resolution mass spectrometry (HRMS), for the determination of PCDDs/PCDFs is described. The potential of using atmospheric-pressure chemical ionization (APCI) coupled to a tandem quadrupole analyzer has been validated for the identification and quantification of dioxins and furans in different complex matrices. The main advantage of using the APCI source is the soft ionization at atmospheric pressure, which results in very limited fragmentation. APCI mass spectra are dominated by the molecular ion cluster, in contrast with the high energy ionization process under electron ionization (El). The use of the molecular ion as the precursor ion in MS/MS enhances selectivity and, consequently, sensitivity by increasing the signal-to-noise ratios (S/N). For standard solutions of 2,3,7,8-TCDD, injections of 10 fg in the splitless mode on 30- or 60-m-length, 0.25 mm inner diameter (id), and 25 mu m film thickness low-polarity capillary columns (DB5MS type), signal-to-noise (S/N) ratios of >10:1 were routinely obtained. Linearity was achieved in the region (correlation coefficient of r(2) > 0.998) for calibration curves ranging from 100 fg/mu L, to 1000 pg/mu L. The results from a wide variety of complex samples, including certified and standard reference materials and samples from several QA/QC studies, which were previously analyzed by EI HRGC/HRMS, were compared with the results from the APGC/MS/MS system. Results between instruments showed good agreement both in individual congeners and toxic equivalence factors (TEQs). The data show that the use of APGC in combination with MS/MS for the analysis of dioxins has the same potential, in terms of sensitivity and selectivity, as the traditional HEMS instrumentation used for this analysis. However, the APCI/MS/MS system, as a benchtop system, is much easier to use.

National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:oru:diva-46035 (URN)10.1021/acs.analchem.5b02264 (DOI)000360773100062 ()26267710 (PubMedID)2-s2.0-84940971553 (Scopus ID)
Note

Funding Agencies:

Generalitat Valenciana, as research group of excellence PROMETEO/2009/054

Serveis Centrals d'Instrumentacio Cientifica (SCIC) of the University Jaume I

China Scholarship Council 201206400003

Available from: 2015-10-07 Created: 2015-10-07 Last updated: 2018-07-02Bibliographically approved
Mullin, L., Burgess, J. A., Jogsten, I. E., Geng, D., Aubin, A. & van Bavel, B. (2015). Rapid separation of hexabromocyclododecane diastereomers using a novel method combining convergence chromatography and tandem mass spectrometry. Analytical Methods, 7(7), 2950-2958
Open this publication in new window or tab >>Rapid separation of hexabromocyclododecane diastereomers using a novel method combining convergence chromatography and tandem mass spectrometry
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2015 (English)In: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 7, no 7, p. 2950-2958Article in journal (Refereed) Published
Abstract [en]

Analysis of the brominated flame retardant hexabromocyclododecane (HBCDD) is characterized by the separation of its three predominant diastereomers. This analysis is typically performed using reversed phase liquid chromatography (RPLC) coupled with mass spectrometric (MS) detection with analysis times in the order of 10 minutes or greater. Here we describe a rapid method using supercritical CO2 and methanol to baseline separate the three most abundant HBCDD diastereomers within a three minute run time using a High Strength Silica (HSS) C18 1.8 mu m particle size column. A unique elution order of the alpha-, beta- and gamma-HBCDD diastereomers using supercritical CO2 was observed, and can be used as an orthogonal separation for further confidence in diastereomer identification when used in conjuction with RPLC. A tandem quadrupole mass spectrometer with negative mode electrospray ionization was used for detection, operating in multiple reaction monitoring (MRM) mode. Ionization was enhanced by the addition of a make-up flow, which was introduced to the post-column effluent. Method limit of detection (LOD) and limit of quantification (LOQ) for alpha-, beta- and gamma-HBCDD were based on peak-to-peak signal to noise ratios of greater than 3 or 10, respectively. The LOD for all HBCDD diastereomers as solvent standards was 100 fg on-column, and LOQs 500 fg on-column for alpha- and gamma-HBCDD and 250 fg on-column for beta-HBCDD. In order to test the efficiency of this method, small subsets of complex human serum and whale blubber extracts were analyzed using this method, resulting in positive detections in samples of alpha-HBCDD.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2015
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
urn:nbn:se:oru:diva-44434 (URN)10.1039/c4ay02923b (DOI)000351931000008 ()
Available from: 2015-04-24 Created: 2015-04-24 Last updated: 2019-10-09Bibliographically approved
Geng, D., Ericson Jogsten, I., Kukucka, P., Hagberg, J., Roos, A. & van Bavel, B. (2014). Comparison on atmospheric pressure gas chromatography-tandem mass spectrometry (APGC-MS/MS) and high resolution mass spectrometry for the analysis of polybrominated diphenyl ethers (PBDEs). Paper presented at 34th International Symposium on Halogenated Persistent Organic Pollutants, Dioxin 2014, Madrid, Spain, August 31 - September 5, 2014. Organohalogen Compounds, 76, 1593-1596
Open this publication in new window or tab >>Comparison on atmospheric pressure gas chromatography-tandem mass spectrometry (APGC-MS/MS) and high resolution mass spectrometry for the analysis of polybrominated diphenyl ethers (PBDEs)
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2014 (English)In: Organohalogen Compounds, ISSN 1026-4892, Vol. 76, p. 1593-1596Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Eco-Informa Press, 2014
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-43699 (URN)
Conference
34th International Symposium on Halogenated Persistent Organic Pollutants, Dioxin 2014, Madrid, Spain, August 31 - September 5, 2014
Available from: 2015-03-17 Created: 2015-03-17 Last updated: 2018-04-05Bibliographically approved
Gauthier, M.-S., Rabasa-Lhoret, R., Prud'homme, D., Karelis, A. D., Geng, D., van Bavel, B. & Ruzzin, J. (2014). The Metabolically Healthy But Obese Phenotype Is Associated With Lower Plasma Levels of Persistent Organic Pollutants as Compared to the Metabolically Abnormal Obese Phenotype. Journal of Clinical Endocrinology and Metabolism, 99(6), E1061-E1066
Open this publication in new window or tab >>The Metabolically Healthy But Obese Phenotype Is Associated With Lower Plasma Levels of Persistent Organic Pollutants as Compared to the Metabolically Abnormal Obese Phenotype
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2014 (English)In: Journal of Clinical Endocrinology and Metabolism, ISSN 0021-972X, E-ISSN 1945-7197, Vol. 99, no 6, p. E1061-E1066Article in journal (Refereed) Published
Abstract [en]

Context: Although obesity is strongly linked to insulin resistance and type 2 diabetes, a subset of obese individuals termed metabolically healthy but obese(MHO) appears relatively protected from the development of cardiometabolic complications. The origins of this metabolically healthy phenotype remain unclear. Recently, persistent organic pollutants (POPs) have emerged as potential endocrine disruptors.

Objective: The aim of this study was to test the hypothesis that the MHO phenotype presents lower circulating levels of POPs as compared to the metabolically abnormal obese (MAO) phenotype.

Design, Setting, and Patients: We conducted a cross-sectional study of 76 nondiabetic obese (body mass index >= 30 kg/m(2)) postmenopausal women.

Main Outcome Measures: Plasma concentrations of 21 POPs as well as cardiometabolic risk factors were analyzed.

Results: For similar age, body mass index, and fat mass index, MHO women (n = 40) showed higher insulin sensitivity levels and a more favorable cardiometabolic profile than MAO women (n = 36), as evidenced by a 2-fold increase in glucose disposal rates measured by the hyperinsulinemic-euglycemic clamp (P = .001). Among 18 detectable pollutants measured, MAO women had higher plasma concentrations of 12 POPs (fold increase, 1.4-2.9; P < .001-.036). Logistic regression analyses showed that the prevalence of the MAO phenotype was significantly associated with higher levels of total dioxin and non-dioxin-like polychlorinated biphenyls (odds ratio, 4.7; 95% confidence interval, 1.8-12.5; P = .002), as well as trans-nonachlor (odds ratio, 6.1; 95% CI, 2.2-16.4; P < .001).

Conclusion: Our study demonstrates that the metabolically healthy and abnormal phenotypes have distinct plasma POP profiles.

National Category
Chemical Sciences
Research subject
Chemistry
Identifiers
urn:nbn:se:oru:diva-38248 (URN)10.1210/jc.2013-3935 (DOI)000342340500018 ()24606089 (PubMedID)2-s2.0-84899864055 (Scopus ID)
Note

Funding Agencies:

Genome Quebec (CAO project)

J-A DeSeve Chair for Clinical Research

Canadian Institutes of Health Research New Emerging Team in Obesity (University of Montreal and University of Ottawa, MONET project) T 0602145.02

Norwegian Diabetes Association

Grieg Foundation

China Scholarship Council 201206400003

Research Council of Norway 228135

Available from: 2014-11-03 Created: 2014-10-30 Last updated: 2018-09-12Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-8553-8824

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