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Meyer-Alert, H., Larsson, M., Hollert, H. & Keiter, S. (2019). Benzo[a]pyrene and 2,3-benzofuran induce divergent temporal patterns of AhR-regulated responses in zebrafish embryos (Danio rerio). Ecotoxicology and Environmental Safety, 183, Article ID UNSP 109505.
Open this publication in new window or tab >>Benzo[a]pyrene and 2,3-benzofuran induce divergent temporal patterns of AhR-regulated responses in zebrafish embryos (Danio rerio)
2019 (English)In: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 183, article id UNSP 109505Article in journal (Refereed) Published
Abstract [en]

Biotests like the fish embryo toxicity test have become increasingly popular in risk assessment and evaluation of chemicals found in the environment. The large range of possible endpoints is a big advantage when researching on the mode of action of a certain substance. Here, we utilized the frequently used model organism zebrafish (Danio rerio) to examine regulative mechanisms in the pathway of the aryl-hydrocarbon receptor (AHR) in early development. We exposed embryos to representatives of two chemical classes known to elicit dioxin-like activity: benzo[a]pyrene for polycyclic aromatic hydrocarbons (PAHs) and 2,3-benzofuran for polar O-substituted heterocycles as a member of heterocyclic compounds in general (N-, S-, O-heterocycles; NSO-hets). We measured gene transcription of the induced P450 cytochromes (cyp1), their formation of protein and biotransformation activity throughout the whole embryonic development until 5 days after fertilization. The results show a very specific time course of transcription depending on the chemical properties (e.g. halogenation, planarity, Kow), the physical decay and the biodegradability of the tested compound. However, although this temporal pattern was not precisely transferable onto the protein level, significant regulation in enzymatic activity over time could be detected. We conclude, that a careful choice of time and end point as well as consideration of the chemical properties of a substance are fairly important when planning, conducting and especially evaluating biotests.

Place, publisher, year, edition, pages
Academic Press, 2019
Keywords
Aryl hydrocarbon receptor, Biotransformation, Cytochrome P450, Dioxin-like activity, EROD, Zebrafish embryo
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:oru:diva-75813 (URN)10.1016/j.ecoenv.2019.109505 (DOI)000487178000011 ()31394372 (PubMedID)2-s2.0-85070090993 (Scopus ID)
Note

Funding Agencies:

German Federal Environment Foundation (DBU)  

EnForce laboratory - Knowledge Foundation  20160019

Available from: 2019-08-23 Created: 2019-08-23 Last updated: 2019-11-15Bibliographically approved
Blanc, M., Rüegg, J., Scherbak, N. & Keiter, S. (2019). Environmental chemicals differentially affect epigenetic-related mechanisms in the zebrafish liver (ZF-L) cell line and in zebrafish embryos. Aquatic Toxicology, 215, Article ID 105272.
Open this publication in new window or tab >>Environmental chemicals differentially affect epigenetic-related mechanisms in the zebrafish liver (ZF-L) cell line and in zebrafish embryos
2019 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 215, article id 105272Article in journal (Refereed) Published
Abstract [en]

A number of chemicals have been shown to affect epigenetic patterning and functions. Since epigenetic mechanisms regulate transcriptional networks, epigenetic changes induced by chemical exposure can represent early molecular events for long-term adverse physiological effects. Epigenetics has thus appeared as a research field of major interest within (eco)toxicological sciences. The present study aimed at measuring effects on epigenetic-related mechanisms of selected environmental chemicals (bisphenols, perfluorinated chemicals, methoxychlor, permethrin, vinclozolin and coumarin 47) in zebrafish embryos and liver cells (ZFL). Transcription of genes related to DNA methylation and histone modifications was measured and global DNA methylation was assessed in ZFL cells using the LUMA assay. The differences in results gathered from both models suggest that chemicals affect different mechanisms related to epigenetics in embryos and cells. In zebrafish embryos, exposure to bisphenol A, coumarin 47, methoxychlor and permethrin lead to significant transcriptional changes in epigenetic factors suggesting that they can impact early epigenome reprogramming related to embryonic development. In ZFL cells, significant transcriptional changes were observed upon exposure to all chemicals but coumarin 47; however, only perfluorooctane sulfonate induced significant effects on global DNA methylation. Notably, in contrast to the other tested chemicals, perfluorooctane sulfonate affected only the expression of the histone demethylase kdm5ba. In addition, kdm5ba appeared as a sensitive gene in zebrafish embryos as well. Taken together, the present results suggest a role for kdm5ba in regulating epigenetic patterns in response to chemical exposure, even though mechanisms remain unclear. To confirm these findings, further evidence is required regarding changes in site-specific histone marks and DNA methylation together with their long-term effects on physiological outcomes.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Chemical pollutant, DNA methylation, Danio rerio, Histone modification, LUMA, qPCR
National Category
Developmental Biology
Identifiers
urn:nbn:se:oru:diva-77033 (URN)10.1016/j.aquatox.2019.105272 (DOI)000489354800005 ()31442592 (PubMedID)2-s2.0-85070872497 (Scopus ID)
Funder
Swedish Research Council Formas
Note

Funding Agency:

EnForce platform - KK Foundation  201660019

Available from: 2019-10-07 Created: 2019-10-07 Last updated: 2019-10-25Bibliographically approved
Alfonso, S., Blanc, M., Joassard, L., Keiter, S., Munschy, C., Loizeau, V., . . . Cousin, X. (2019). Examining multi- and transgenerational behavioral and molecular alterations resulting from parental exposure to an environmental PCB and PBDE mixture. Aquatic Toxicology, 208, 29-38
Open this publication in new window or tab >>Examining multi- and transgenerational behavioral and molecular alterations resulting from parental exposure to an environmental PCB and PBDE mixture
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2019 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 208, p. 29-38Article in journal (Refereed) Published
Abstract [en]

Polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants extensively used during the 20th century and still present in aquatic environments despite their ban. Effects of exposure to these compounds over generations are poorly documented. Therefore, our aims were to characterize behavioral responses and underlying molecular mechanisms in zebrafish exposed to an environmentally relevant mixture of PCBs and PBDEs as well as in four unexposed offspring generations. Zebrafish (F0) were chronically exposed from the first meal onward to a diet spiked with a mixture containing 22 PCB and 7 PBDE congeners in proportions and concentrations reflecting environmental situations (ΣPCBs = 1991 and ΣPBDEs = 411 ng/g). Four offspring generations (F1 to F4) were obtained from this F0 and were not further exposed. Behavior was assessed at both larval and adult stages. Mechanisms related to behavioral defects (habenula maturation and c-fos transcription) and methylation (dnmts transcription) were monitored in larvae. Exposed adult F0 as well as F1 and F3 adults displayed no behavioral change while F2 expressed anxiety-like behavior. Larval behavior was also disrupted, i.e. hyperactive after light to dark transition in F1 or hypoactive in F2, F3 and F4. Behavioral disruptions may be related to defect in habenula maturation (observed in F1) and change in c-fos transcription (observed in F1 and F2). Transcription of the gene encoding DNA methyltransferase (dnmt3ba) was also modified in all generations. Our results lead us to hypothesize that chronic dietary exposure to an environmentally relevant mixture of PCB and PBDE triggers multigenerational and transgenerational molecular and behavioral disruptions in a vertebrate model.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Behavior, Epigenetic, Gene transcription, Offspring, Parental exposure, Zebrafish
National Category
Developmental Biology Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-71178 (URN)10.1016/j.aquatox.2018.12.021 (DOI)000459950500004 ()30605867 (PubMedID)2-s2.0-85059240354 (Scopus ID)
Note

Funding Agencies:

French National Research Agency, project Fish'N'POPs  ANR-13-CESA-020 

Ifremer 

Available from: 2019-01-08 Created: 2019-01-08 Last updated: 2019-06-18Bibliographically approved
Cormier, B., Batel, A., Cachot, J., Begout, M.-L., Braunbeck, T., Cousin, X. & Keiter, S. (2019). Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio). Frontiers in Environmental Science, 7, Article ID 135.
Open this publication in new window or tab >>Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)
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2019 (English)In: Frontiers in Environmental Science, E-ISSN 2296-665X, Vol. 7, article id 135Article in journal (Refereed) Published
Abstract [en]

As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a] pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019
Keywords
fish embryotoxicity test (FET), swimming behavior, EROD, cyp1a, perfluorooctane sulfonate, benzo[a]pyrene, oxybenzone
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-76995 (URN)10.3389/fenvs.2019.00135 (DOI)000486181200001 ()
Note

Funding Agencies:

JPI Oceans  FCT JPIOCEANS/0005/2015 ANR-15-JOCE-0002-01 BMBF 03F0735A

University of Bordeaux  

German Academic Scholarship Foundation (Studienstiftung des Deutschen Volkes)  

JPI Oceans (FORMAS)  2015-01865

Available from: 2019-10-03 Created: 2019-10-03 Last updated: 2019-10-03Bibliographically approved
Meyer-Alert, H., Ladermann, K., Larsson, M., Schiwy, S., Hollert, H. & Keiter, S. H. (2018). A temporal high-resolution investigation of the Ah-receptor pathway during early development of zebrafish (Danio rerio). Aquatic Toxicology, 204, 117-129
Open this publication in new window or tab >>A temporal high-resolution investigation of the Ah-receptor pathway during early development of zebrafish (Danio rerio)
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2018 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 204, p. 117-129Article in journal (Refereed) Published
Abstract [en]

In order to contribute to a comprehensive understanding of the regulating mechanisms of the aryl-hydrocarbon-receptor (AHR) in zebrafish embryos, we aimed to elucidate the interaction of proteins taking part in this signaling pathway during early development of the zebrafish (Danio rerio) after chemical exposure. We managed to illustrate initial transcription processes of the implemented proteins after exposure to two environmentally relevant chemicals: polychlorinated biphenyl 126 (PCB126) and β-Naphthoflavone (BNF). Using qPCR, we quantified mRNA every 4 h until 118 h post fertilization and found the expression of biotransformation enzymes (cyp1 family) and the repressor of the AHR (ahr-r) to be dependent on the duration of chemical exposure and the biodegradability of the compounds. PCB126 induced persistently increased amounts of transcripts as it is not metabolized, whereas activation by BNF was limited to the initial period of exposure. We did not find a clear relation between the amount of transcripts and activity of the induced CYP-proteins, so posttranscriptional mechanisms are likely to regulate biotransformation of BNF. With regard to zebrafish embryos and their application in risk assessment of hazardous chemicals, our examination of the AHR pathway especially supports the relevance of the time point or period of exposure that is used for bioanalytical investigations and consideration of chemical properties determining biodegradability.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Arylhydrocarbon receptor, Biotransformation, Cytochrome P450, Polychlorinated biphenyl 126, Zebrafish embryo, β-Naphthoflavone
National Category
Biochemistry and Molecular Biology Developmental Biology
Identifiers
urn:nbn:se:oru:diva-69587 (URN)10.1016/j.aquatox.2018.09.007 (DOI)000449240800012 ()30245344 (PubMedID)2-s2.0-85053782365 (Scopus ID)
Note

Funding Agencies:

German Federal Environment Foundation (DBU)  

European FP7 Collaborative Project SOLUTIONS  603437 

Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2018-11-20Bibliographically approved
Legradi, J. B., Di Paolo, C., Kraak, M. H., van der Geest, H. G., Schymanski, E. L., Williams, A. J., . . . Hollert, H. (2018). An ecotoxicological view on neurotoxicity assessment. Environmental Sciences Europe, 30, Article ID 46.
Open this publication in new window or tab >>An ecotoxicological view on neurotoxicity assessment
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2018 (English)In: Environmental Sciences Europe, ISSN 2190-4707, E-ISSN 2190-4715, Vol. 30, article id 46Article, review/survey (Refereed) Published
Abstract [en]

The numbers of potential neurotoxicants in the environment are raising and pose a great risk for humans and the environment. Currently neurotoxicity assessment is mostly performed to predict and prevent harm to human populations. Despite all the efforts invested in the last years in developing novel in vitro or in silico test systems, in vivo tests with rodents are still the only accepted test for neurotoxicity risk assessment in Europe. Despite an increasing number of reports of species showing altered behaviour, neurotoxicity assessment for species in the environment is not required and therefore mostly not performed. Considering the increasing numbers of environmental contaminants with potential neurotoxic potential, eco-neurotoxicity should be also considered in risk assessment. In order to do so novel test systems are needed that can cope with species differences within ecosystems. In the field, online-biomonitoring systems using behavioural information could be used to detect neurotoxic effects and effect-directed analyses could be applied to identify the neurotoxicants causing the effect. Additionally, toxic pressure calculations in combination with mixture modelling could use environmental chemical monitoring data to predict adverse effects and prioritize pollutants for laboratory testing. Cheminformatics based on computational toxicological data from in vitro and in vivo studies could help to identify potential neurotoxicants. An array of in vitro assays covering different modes of action could be applied to screen compounds for neurotoxicity. The selection of in vitro assays could be guided by AOPs relevant for eco-neurotoxicity. In order to be able to perform risk assessment for eco-neurotoxicity, methods need to focus on the most sensitive species in an ecosystem. A test battery using species from different trophic levels might be the best approach. To implement eco-neurotoxicity assessment into European risk assessment, cheminformatics and in vitro screening tests could be used as first approach to identify eco-neurotoxic pollutants. In a second step, a small species test battery could be applied to assess the risks of ecosystems.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
Eco-neurotoxicity, Neurotoxicity, EDA, REACH, AOP, Behaviour, Computational toxicity, Ecological, Species
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-71139 (URN)10.1186/s12302-018-0173-x (DOI)000453261000001 ()2-s2.0-85058931720 (Scopus ID)
Note

Funding Agencies:

Federal Ministry of Education and Research (BMBF)  

Norman Network  

Dutch Water companies  BTO2018-2023 

SOLUTIONS project (European Union)  603437 

Available from: 2019-01-08 Created: 2019-01-08 Last updated: 2019-01-08Bibliographically approved
O’Donovan, S., Mestre, N. C., Abel, S., Fonseca, T. G., Carteny, C. C., Cormier, B., . . . Bebianno, M. J. (2018). Ecotoxicological Effects of Chemical Contaminants Adsorbed to Microplastics in the Clam Scrobicularia plana. Frontiers in Marine Science, 5, Article ID 143.
Open this publication in new window or tab >>Ecotoxicological Effects of Chemical Contaminants Adsorbed to Microplastics in the Clam Scrobicularia plana
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2018 (English)In: Frontiers in Marine Science, E-ISSN 2296-7745, Vol. 5, article id 143Article in journal (Refereed) Published
Abstract [en]

Although microplastics are distributed globally in the marine environment, a great deal of unknowns relating to their ecotoxicological effects on the marine biota remain. Due to their lipophilic nature, microplastics have the potential to adsorb persistent organic pollutants present in contaminated regions, which may increase their detrimental impact once assimilated by organisms. This study investigates the ecotoxicological effects of exposure to low-density polyethylene (LDPE) microplastics (11 - 13 µm), with and without adsorbed contaminants (benzo[a]pyrene - BaP and perfluorooctane sulfonic acid - PFOS), in the peppery furrow shell clam, Scrobicularia plana. Environmentally relevant concentrations of contaminants (BaP - 16.87±0.22 µg g-1 and PFOS - 70.22±12.41 µg g-1) were adsorbed to microplastics to evaluate the potential role of plastic particles as a source of chemical contamination once ingested. S. plana were exposed to microplastics, at a concentration of 1 mg L-1, in a water-sediment exposure setup for 14 days. Clams were sampled at the beginning of the experiment (day 0) and after 3, 7 and 14 days. BaP accumulation, in whole clam tissues, was analysed. A multi-biomarker assessment was conducted in the gills, digestive gland, and haemolymph of clams to clarify the effects of exposure. This included the quantification of antioxidant (superoxide dismutase, catalase, glutathione peroxidase) and biotransformation (glutathione-S-transferases) enzyme activities, oxidative damage (lipid peroxidation levels), genotoxicity (single and double strand DNA breaks), and neurotoxicity (acetylcholinesterase activity). Results suggest a potential mechanical injury of gills caused by ingestion of microplastics that may also affect the analysed biomarkers. The digestive gland seems less affected by mechanical damage caused by virgin microplastic exposure, with the MP-adsorbed BaP and PFOS exerting a negative influence over the assessed biomarkers in this tissue.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2018
Keywords
polyethylene, benzo[a]pyrene, perfluorooctane sulfonic acid, DNA damage, oxidative stress, neurotoxicity, oxidative damage
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-69513 (URN)10.3389/fmars.2018.00143 (DOI)000457085600001 ()2-s2.0-85046668101 (Scopus ID)
Note

Funding Agencies:

JPI Oceans  FCT JPIOCEANS/0005/2015 

JPI Oceans (BELSPO)  

JPI Oceans (FWO)  

JPI Oceans (FORMAS)  

JPI Oceans (SwAM)  

FCT  UID/MAR/00350/2013 

Available from: 2019-01-12 Created: 2019-01-12 Last updated: 2019-02-13Bibliographically approved
Bour, A., Haarr, A., Keiter, S. & Hylland, K. (2018). Environmentally relevant microplastic exposure affects sediment-dwelling bivalves. Environmental Pollution, 236, 652-660
Open this publication in new window or tab >>Environmentally relevant microplastic exposure affects sediment-dwelling bivalves
2018 (English)In: Environmental Pollution, ISSN 0269-7491, E-ISSN 1873-6424, Vol. 236, p. 652-660Article in journal (Refereed) Published
Abstract [en]

Most microplastics are expected to sink and end up in marine sediments. However, very little is known concerning their potential impact on sediment-dwelling organisms. We studied the long-term impact of microplastic exposure on two sediment-dwelling bivalve species. Ennucula tenuis and Abra nitida were exposed to polyethylene microparticles at three concentrations (1; 10 and 25 mg/kg of sediment) for four weeks. Three size classes (4-6; 20-25 and 125-500 mu m) were used to study the influence of size on microplastic ecotoxicity. Microplastic exposure did not affect survival, condition index or burrowing behaviour in either bivalve species. However, significant changes in energy reserves were observed. No changes were observed in protein, carbohydrate or lipid contents in E. tenuis, with the exception of a decrease in lipid content for one condition. However, total energy decreased in a dose-dependent manner for bivalves exposed to the largest particles. To the contrary, no significant changes in total energy were observed for A. nitida, although a significant decrease of protein content was observed for individuals exposed to the largest particles, at all concentrations. Concentration and particle size significantly influenced microplastic impacts on bivalves, the largest particles and higher concentrations leading to more severe effects. Several hypotheses are presented to explain the observed modulation of energy reserves, including the influence of microplastic size and concentration. Our results suggest that long-term exposure to microplastics at environmentally relevant concentrations can impact marine benthic biota.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Polyethylene, Ecotoxicity, Benthic bivalves, Environmental conditions, Energy reserves
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-66732 (URN)10.1016/j.envpol.2018.02.006 (DOI)000429187500068 ()29433106 (PubMedID)2-s2.0-85041509457 (Scopus ID)
Note

Funding Agencies:

EU JPI-Oceans programme [project EPHEMARE]  

Research Council of Norway  257902 

Available from: 2018-04-25 Created: 2018-04-25 Last updated: 2018-04-25Bibliographically approved
Hausen, J., Otte, J. C., Legradi, J., Yang, L., Strähle, U., Fenske, M., . . . Ottermanns, R. (2018). Fishing for contaminants: identification of three mechanism specific transcriptome signatures using Danio rerio embryos. Environmental science and pollution research international, 25(5), 4023-4036
Open this publication in new window or tab >>Fishing for contaminants: identification of three mechanism specific transcriptome signatures using Danio rerio embryos
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2018 (English)In: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 25, no 5, p. 4023-4036Article in journal (Refereed) Published
Abstract [en]

In ecotoxicology, transcriptomics is an effective way to detect gene expression changes in response to environmental pollutants. Such changes can be used to identify contaminants or contaminant classes and can be applied as early warning signals for pollution. To do so, it is important to distinguish contaminant-specific transcriptomic changes from genetic alterations due to general stress. Here we present a first step in the identification of contaminant class-specific transcriptome signatures. Embryos of zebrafish (Danio rerio) were exposed to three substances (methylmercury, chlorpyrifos and Aroclor 1254, each from 24 to 48 hpf exposed) representing sediment typical contaminant classes. We analyzed the altered transcriptome to detect discriminative genes significantly regulated in reaction to the three applied contaminants. By comparison of the results of the three contaminants, we identified transcriptome signatures and biologically important pathways (using Cytoscape/ClueGO software) that react significantly to the contaminant classes. This approach increases the chance of finding genes that play an important role in contaminant class-specific pathways rather than more general processes.

Place, publisher, year, edition, pages
Springer, 2018
Keywords
Transcriptomics; Methylmercury; Aroclor 1254; Chlorpyrifos; Ecotoxicogenomics; Pathway network analysis
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-57329 (URN)10.1007/s11356-017-8977-6 (DOI)000425770300009 ()28391457 (PubMedID)2-s2.0-85017145945 (Scopus ID)
Note

Funding Agencies:

DanTox  

German Federal Ministry of Education and Research (BMBF)  02WU1053 

GENDarT2 project (BMBF)  AZ:0315190 B 

Available from: 2017-05-30 Created: 2017-05-30 Last updated: 2018-03-21Bibliographically approved
Beiras, R., Bellas, J., Cachot, J., Cormier, B., Cousin, X., Engwall, M., . . . Vidal-Liñán, L. (2018). Ingestion and contact with polyethylene microplastics does not cause acute toxicity on marine zooplankton. Journal of Hazardous Materials, 360, 452-460
Open this publication in new window or tab >>Ingestion and contact with polyethylene microplastics does not cause acute toxicity on marine zooplankton
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2018 (English)In: Journal of Hazardous Materials, ISSN 0304-3894, E-ISSN 1873-3336, Vol. 360, p. 452-460Article in journal (Refereed) Published
Abstract [en]

Toxicity of polyethylene microplastics (PE-MP) of size ranges similar to their natural food to zooplanktonic organisms representative of the main taxa present in marine plankton, including rotifers, copepods, bivalves, echinoderms and fish, was evaluated. Early life stages (ELS) were prioritized as testing models in order to maximize sensitivity. Treatments included particles spiked with benzophenone-3 (BP-3), a hydrophobic organic chemical used in cosmetics with direct input in coastal areas. Despite documented ingestion of both virgin and BP-3 spiked microplastics no acute toxicity was found at loads orders of magnitude above environmentally relevant concentrations on any of the invertebrate models. In fish tests some effects, including premature or reduced hatching, were observed after 12 d exposure at 10 mg L-1 of BP-3 spiked PE-MP. The results obtained do not support environmentally relevant risk of microplastics on marine zooplankton. Similar approaches testing more hydrophobic chemicals with higher acute toxicity are needed before these conclusions could be extended to other organic pollutants common in marine ecosystems. Therefore, the replacement of these polymers in consumer products must be carefully considered.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Benzophenone-3, Embryo-larval bioassays, Marine litter, Marine zooplankton, Polyethylene
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-69565 (URN)10.1016/j.jhazmat.2018.07.101 (DOI)000446144600050 ()30142596 (PubMedID)2-s2.0-85051825465 (Scopus ID)
Funder
Swedish Research Council Formas
Note

Funding Agencies:

Spanish Government (MINECO)  PCIN-2015-187-C03-03  CTM2016-77945-C3 

French National Research Agency (Agence National de La Recherche)  ANR-15-JOCE-0002-05 

Galician Government  ED431C 2017/46 

Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2018-10-17Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-2356-6686

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