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Nilén, G., Larsson, M., Hyötyläinen, T. & Keiter, S. (2024). A complex mixture of polycyclic aromatic compounds causes embryotoxic, behavioral, and molecular effects in zebrafish larvae (Danio rerio), and in vitro bioassays. Science of the Total Environment, 906, Article ID 167307.
Open this publication in new window or tab >>A complex mixture of polycyclic aromatic compounds causes embryotoxic, behavioral, and molecular effects in zebrafish larvae (Danio rerio), and in vitro bioassays
2024 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 906, article id 167307Article in journal (Refereed) Published
Abstract [en]

Polycyclic aromatic compounds (PACs) are prevalent in the environment, typically found in complex mixtures and high concentrations. Our understanding of the effects of PACs, excluding the 16 priority polycyclic aromatic hydrocarbons (16 PAHs), remains limited. Zebrafish embryos and in vitro bioassays were utilized to investigate the embryotoxic, behavioral, and molecular effects of a soil sample from a former gasworks site in Sweden. Additionally, targeted chemical analysis was conducted to analyze 87 PACs in the soil, fish, water, and plate material. CALUX® assays were used to assess the activation of aryl hydrocarbon and estrogen receptors, as well as the inhibition of the androgen receptor. Larval behavior was measured by analyzing activity during light and darkness and in response to mechanical stimulation. Furthermore, qPCR analyses were performed on a subset of 36 genes associated with specific adverse outcomes, and the total lipid content in the larvae was measured. Exposure to the sample resulted in embryotoxic effects (LC50 = 0.480 mg dry matter soil/mL water). The mixture also induced hyperactivity in darkness and hypoactivity in light and in response to the mechanical stimulus. qPCR analysis revealed differential regulation of 15 genes, including downregulation of opn1sw1 (eye pigmentation) and upregulation of fpgs (heart failure). The sample caused significant responses in three bioassays (ERα-, DR-, and PAH-CALUX), and the exposed larvae exhibited elevated lipid levels. Chemical analysis identified benzo[a]pyrene as the predominant compound in the soil and approximately half of the total PAC concentration was attributed to the 16 PAHs. This study highlights the value of combining in vitro and in vivo methods with chemical analysis to assess toxic mechanisms at specific targets and to elucidate the possible interactions between various pathways in an organism. It also enhances our understanding of the risks associated with environmental mixtures of PACs and their distribution during toxicity testing.

Place, publisher, year, edition, pages
Elsevier, 2024
Keywords
Chemical analysis, Environment, Gasworks, Lipidomics, Quantitative gene expression, Soil
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-108825 (URN)10.1016/j.scitotenv.2023.167307 (DOI)001102592900001 ()37804991 (PubMedID)2-s2.0-85173583428 (Scopus ID)
Funder
Knowledge Foundation, 201660019
Available from: 2023-10-10 Created: 2023-10-10 Last updated: 2023-12-15Bibliographically approved
Espinosa-Ruiz, C., González-Fernández, C., Cormier, B., Keiter, S. H., Vieira, L. R., Guilhermino, L., . . . Cuesta, A. (2023). Immunotoxicological effects of perfluorooctanesulfonic acid on European seabass are reduced by polyethylene microplastics. Fish and Shellfish Immunology, 137, Article ID 108793.
Open this publication in new window or tab >>Immunotoxicological effects of perfluorooctanesulfonic acid on European seabass are reduced by polyethylene microplastics
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2023 (English)In: Fish and Shellfish Immunology, ISSN 1050-4648, E-ISSN 1095-9947, Vol. 137, article id 108793Article in journal (Refereed) Published
Abstract [en]

Marine environments receive plastic waste, where it suffers a transformation process into smaller particles. Among them, microplastics (MPs; <5 mm) are ingested by aquatic organisms leading to negative effects on animal welfare. The interactions between MPs, contaminants and organisms are poorly understood. To clarify this issue, European seabass (Dicentrarchus labrax L.) were fed with diets supplemented with 0 (control), polyethylene (PE) MPs (100 mg/kg diet), perfluorooctanesulfonic acid (PFOS, 4.83 μg/kg diet) or PFOS adsorbed to MPs (MPs-PFOS; final concentrations of 4.83 μg and 100 mg of PFOS and MP per kg of feed, respectively). Samples of skin mucus, serum, head-kidney (HK), liver, muscle, brain and intestine were obtained. PFOS levels were high in the liver of fish fed with the PFOS-diet, and markedly reduced when adsorbed to MPs. Compared to the control groups, liver EROD activity did not show any significant changes, whereas brain and muscle cholinesterase activities were decreased in all the groups. The histological and morphometrical study on liver and intestine showed significant alterations in fish fed with the experimental diets. At functional level, all the experimental diets affected the humoral (peroxidase, IgM, protease and bactericidal activities) as well as cellular (phagocytosis, respiratory burst and peroxidase) activities of HK leukocytes, being more marked those effects caused by the PFOS diet. Besides, treatments produced inflammation and oxidative stress as evidenced at gene level. Principal component analysis demonstrated that seabass fed with MPs-PFOS showed more similar effects to MPs alone than to PFOS. Overall, seabass fed with MPs-PFOS diet showed similar or lower toxicological alterations than those fed with MPs or PFOS alone demonstrating the lack of additive effects or even protection against PFOS toxicity.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
European seabass, Immunity, Microplastics, Oxidative stress, PFOS, Polyethylene
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-105863 (URN)10.1016/j.fsi.2023.108793 (DOI)000998727100001 ()37146847 (PubMedID)2-s2.0-85156181052 (Scopus ID)
Note

Funding agencies:

EPHEMARE project (JPI Oceans)

Spanish Government PCIN-2015-187-C03-02

Ministerio de Ciencia e Innovacio'n-Agencia Estatal de Investigacion (MCIN/AEI) PID2019-105522GB-I00

Fundacion Seneca 19883/GERM/15

Fundacao para a Ciencia e a Tecnologia (FCT) JPIOCEANS/0004/2015

Spanish national postdoctoral program Juan de la Cierva Incorporacion JC2019-040510-I

 

Available from: 2023-05-08 Created: 2023-05-08 Last updated: 2023-06-20Bibliographically approved
Fallet, M., Blanc, M., Di Criscio, M., Antczak, P., Engwall, M., Guerrero Bosagna, C., . . . Keiter, S. (2023). Present and future challenges for the investigation of transgenerational epigenetic inheritance. Environment International, 172, Article ID 107776.
Open this publication in new window or tab >>Present and future challenges for the investigation of transgenerational epigenetic inheritance
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2023 (English)In: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Vol. 172, article id 107776Article, review/survey (Refereed) Published
Abstract [en]

Epigenetic pathways are essential in different biological processes and in phenotype-environment interactions in response to different stressors and they can induce phenotypic plasticity. They encompass several processes that are mitotically and, in some cases, meiotically heritable, so they can be transferred to subsequent generations via the germline. Transgenerational Epigenetic Inheritance (TEI) describes the phenomenon that phenotypic traits, such as changes in fertility, metabolic function, or behavior, induced by environmental factors (e.g., parental care, pathogens, pollutants, climate change), can be transferred to offspring generations via epigenetic mechanisms. Investigations on TEI contribute to deciphering the role of epigenetic mechanisms in adaptation, adversity, and evolution. However, molecular mechanisms underlying the transmission of epigenetic changes between generations, and the downstream chain of events leading to persistent phenotypic changes, remain unclear. Therefore, inter-, (transmission of information between parental and offspring generation via direct exposure) and transgenerational (transmission of information through several generations with disappearance of the triggering factor) consequences of epigenetic modifications remain major issues in the field of modern biology. In this article, we review and describe the major gaps and issues still encountered in the TEI field: the general challenges faced in epigenetic research; deciphering the key epigenetic mechanisms in inheritance processes; identifying the relevant drivers for TEI and implement a collaborative and multi-disciplinary approach to study TEI. Finally, we provide suggestions on how to overcome these challenges and ultimately be able to identify the specific contribution of epigenetics in transgenerational inheritance and use the correct tools for environmental science investigation and biomarkers identification.

Place, publisher, year, edition, pages
Elsevier, 2023
Keywords
Adaptation, DNA methylation, Evolution, Non-coding RNAs, Phenotypic plasticity, Post-translational histone modifications
National Category
Genetics
Identifiers
urn:nbn:se:oru:diva-104026 (URN)10.1016/j.envint.2023.107776 (DOI)000927403300001 ()36731188 (PubMedID)2-s2.0-85147212319 (Scopus ID)
Funder
Swedish Research Council Formas, 2019-00510Swedish Research Council, 2021-05245
Available from: 2023-02-03 Created: 2023-02-03 Last updated: 2024-01-02Bibliographically approved
Bebianno, M. J., Mendes, V. M., O'Donovan, S., Carteny, C. C., Keiter, S. & Manadas, B. (2022). Effects of microplastics alone and with adsorbed benzo(a)pyrene on the gills proteome of Scrobicularia plana. Science of the Total Environment, 842, Article ID 156895.
Open this publication in new window or tab >>Effects of microplastics alone and with adsorbed benzo(a)pyrene on the gills proteome of Scrobicularia plana
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2022 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 842, article id 156895Article in journal (Refereed) Published
Abstract [en]

Microplastics (MPs) are globally present in the marine environment, but the biological effects on marine organisms at the molecular and cellular levels remain scarce. Due to their lipophilic nature, MPs can adsorb other contaminants present in the marine environment, which may increase their detrimental effects once ingested by organisms. This study investigates the effects of low-density polyethylene (PE) MPs with and without adsorbed benzo[a]pyrene in the gills proteome of the peppery furrow shell clam, Scrobicularia plana. Clams were exposed to PE MPs (11-13 μm; 1 mg L-1) for 14 days. BaP was analyzed in whole clams' soft tissues, and a proteomic approach was applied in the gills using SWATH/DIA analysis. Proteomic responses suggest that virgin MPs cause disturbance by altering cytoskeleton and cell structure, energy metabolism, conformational changes, oxidative stress fatty acid, DNA binding and, neurotransmission highlighting the potential risk of this type of MPs for the clam health. Conversely, when clam gills were exposed to MPs adsorbed with BaP a higher differentiation of protein expression was observed that besides changes in cytoskeleton and cell structure, oxidative stress, energy metabolism and DNA binding also induce changes in glucose metabolism, RNA binding and apoptosis. These results indicate that the presence of both stressors (MPs and BaP) have a higher toxicological risk to the health of S. plana.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Bivalves, Microplastics, Polyethylene, Proteomics, Scrobicularia plana
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-99784 (URN)10.1016/j.scitotenv.2022.156895 (DOI)000823295200005 ()35753444 (PubMedID)2-s2.0-85133432069 (Scopus ID)
Note

Funding agencies:

Portuguese Foundation for Science and Technology JPIOCEANS/0005/2015 JPIOCEANS/0005/2020  

European Regional Development Fund (ERDF) through the COMPETE 2020 -Operational Programme for Competitiveness and Internationalization

Portuguese Foundation for Science and Technology POCI-01-0145-FEDER-007440 UIDB/04539/2020 UIDP/04539/2020  

National Mass Spectrometry Network (RNEM) - FEDER CRESC Algarve (P02020) POCI-01-0145-FEDER-402-022125 ROTEIRO/0028/2013 PTDC/BIA-BMA/30922/2017 ALG-010145-FEDER-030922 PTDC/BIA-BMA/30922/2017 

Portuguese Foundation for Science and Technology European Commission LA/P/0069/2020 UID/00350/2020

Available from: 2022-06-28 Created: 2022-06-28 Last updated: 2022-08-01Bibliographically approved
Rodrigues, A. R., Mestre, N. C. C., Fonseca, T. G., Pedro, P. Z., Carteny, C. C., Cormier, B., . . . Bebianno, M. J. (2022). Influence of Particle Size on Ecotoxicity of Low-Density Polyethylene Microplastics, with and without Adsorbed Benzo-a-Pyrene, in Clam Scrobicularia plana. Biomolecules, 12(1), Article ID 78.
Open this publication in new window or tab >>Influence of Particle Size on Ecotoxicity of Low-Density Polyethylene Microplastics, with and without Adsorbed Benzo-a-Pyrene, in Clam Scrobicularia plana
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2022 (English)In: Biomolecules, E-ISSN 2218-273X, Vol. 12, no 1, article id 78Article in journal (Refereed) Published
Abstract [en]

This study investigated the ecotoxicological effects of differently sized (4-6 µm and 20-25 µm) low-density polyethylene (LDPE) microplastics (MPs), with and without adsorbed benzo-a-pyrene (BaP), in clam Scrobicularia plana. Biomarkers of oxidative stress (superoxide dismutase-SOD; catalase-CAT), biotransformation (glutathione-S-transferases-GST), oxidative damage (lipid peroxidation-LPO) and neurotoxicity (acetylcholinesterase-AChE) were analysed in gills and digestive glands at different time intervals for a total of 14 days of exposure. In order to have a better impact perspective of these contaminants, an integrated biomarker response index (IBR) and Health Index were applied. Biomarker alterations are apparently more related to smaller sized (4-6 µm) MPs in gills and to virgin LDPE MPs in the digestive gland according to IBR results, while the digestive gland was more affected by these MPs according to the health index.

Place, publisher, year, edition, pages
MDPI, 2022
Keywords
IBR, Scrobicularia plana, benzo-a pyrene, biomarkers, health index, microplastics
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-96833 (URN)10.3390/biom12010078 (DOI)000758128100001 ()35053226 (PubMedID)2-s2.0-85122123962 (Scopus ID)
Note

Funding agencies:

National funding agencies in the framework of JPI Oceans (Fundacao para a Ciencia e Tecnologia (FCT), Portugal JPIOCEANS/0005/2015

FCT through University of Algarve UID/00350/2020

Available from: 2022-01-31 Created: 2022-01-31 Last updated: 2022-03-03Bibliographically approved
Nilén, G., Obamwonyi, O. S., Liem-Nguyen, V., Engwall, M., Larsson, M. & Keiter, S. (2022). Observed and predicted embryotoxic and teratogenic effects of organic and inorganic environmental pollutants and their mixtures in zebrafish (Danio rerio). Aquatic Toxicology, 248, Article ID 106175.
Open this publication in new window or tab >>Observed and predicted embryotoxic and teratogenic effects of organic and inorganic environmental pollutants and their mixtures in zebrafish (Danio rerio)
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2022 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 248, article id 106175Article in journal (Refereed) Published
Abstract [en]

Risk assessment of chemicals is still primarily focusing on single compound evaluation, even if environmental contamination consists of a mixture of pollutants. The concentration addition (CA) and independent action (IA) models have been developed to predict mixture toxicity. Both models assume no interaction between the components, resulting in an additive mixture effect. In the present study, the embryo toxicity test (OECD TG no. 236) with zebrafish embryos (Danio rerio) was performed to investigate whether the toxicity caused by binary, ternary, and quaternary mixtures of organic (Benzo[a]pyrene, perfluorooctanesulfonate, and 3,3´,4,4´,5-pentachlorobiphenyl 126) and inorganic (arsenate) pollutants can be predicted by CA and IA. The acute toxicity and sub-lethal alterations such as lack of blood circulation were investigated. The models estimated the mixture toxicity well and most of the mixtures were additive. However, the binary mixture of PFOS and PCB126 caused a synergistic effect, with almost a ten-fold difference between the observed and predicted LC50-value. For most of the mixtures, the CA model was better in predicting the mixture toxicity than the IA model, which was not expected due to the chemicals' different modes of action. In addition, some of the mixtures caused sub-lethal effects not observed in the single compound toxicity tests. The mixture of PFOS and BaP caused a division of the yolk and imbalance was caused by the combination of PFOS and As and the ternary mixture of PFOS, As, and BaP. Interestingly, PFOS was part of all three mixtures causing the mixture specific sub-lethal effects. In conclusion, the present study shows that CA and IA are mostly resulting in good estimations of the risks that mixtures with few components are posing. However, for a more reliable assessment and a better understanding of mixture toxicity, further investigations are required to study the underlying mechanisms.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Binary mixtures, Concentration addition, Independent action, Mixture toxicity, Mixtures, Quaternary mixture, Ternary
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-98901 (URN)10.1016/j.aquatox.2022.106175 (DOI)000817770000002 ()35523058 (PubMedID)2-s2.0-85129524208 (Scopus ID)
Note

Funding agency:

General Electric 201660019

Available from: 2022-05-09 Created: 2022-05-09 Last updated: 2023-10-31Bibliographically approved
Cormier, B., Borchet, F., Kärrman, A., Szot, M., Yeung, L. W. Y. & Keiter, S. (2022). Sorption and desorption kinetics of PFOS to pristine microplastic. Environmental Science and Pollution Research, 29(3), 4497-4507
Open this publication in new window or tab >>Sorption and desorption kinetics of PFOS to pristine microplastic
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2022 (English)In: Environmental Science and Pollution Research, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 29, no 3, p. 4497-4507Article in journal (Refereed) Published
Abstract [en]

The sorption processes of persistent organic pollutants on microplastics particles are poorly understood. Therefore, the present study investigated the sorption processes of perfluorooctanesulfonate (PFOS) on polyethylene (PE) microplastic particles (MPs) which are representing a prominent environmental pollutant and one of the most abundant microplastic polymers in the aquatic environment, respectively. The focus was set on the investigation of the impact of the particle size on PFOS sorption using four different PE MPs size ranges. The sorption kinetics for 6 months was studied with one selected size range of PE MPs. Besides, the desorption of PFOS from PE MPs under simulated digestive conditions was carried out by using artificial gut fluid mimicking the intestinal juice of fish. The investigation of the size effects of particles over 6 months demonstrated a linear increase of PFOS concentration sorbed onto PE with a decrease of the particle size. Thus, our findings implicate efficient sorption of PFOS onto PE MPs of different sizes. The results showed that PFOS desorbed from the PE MPs into the artificial gut fluid with a rate of 70 to 80%. Besides, a longer exposure of PE MPs to PFOS leads to a higher concentration adsorbed by PE MPs, which may favor the ingestion of higher concentration of PFOS, and thus represents a higher risk to transfer relevant concentrations of PFOS during digestion.

Place, publisher, year, edition, pages
Springer, 2022
Keywords
Microplastics, PFOS, Polyethylene, Sorption
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-93803 (URN)10.1007/s11356-021-15923-x (DOI)000686072700008 ()34409531 (PubMedID)2-s2.0-85112806294 (Scopus ID)
Funder
Swedish Research Council Formas, 2015-01865Knowledge Foundation, 201660019
Note

Funding agencies:

Örebro University

JPI Oceans FCT JPIOCEANS/0005/2015

IdEx grant from University of Bordeaux

Available from: 2021-08-20 Created: 2021-08-20 Last updated: 2022-02-03Bibliographically approved
Cormier, B., Le Bihanic, F., Cabar, M., Crebassa, J.-C., Blanc, M., Larsson, M., . . . Cousin, X. (2021). Chronic feeding exposure to virgin and spiked microplastics disrupts essential biological functions in teleost fish. Journal of Hazardous Materials, 415, Article ID 125626.
Open this publication in new window or tab >>Chronic feeding exposure to virgin and spiked microplastics disrupts essential biological functions in teleost fish
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2021 (English)In: Journal of Hazardous Materials, ISSN 0304-3894, E-ISSN 1873-3336, Vol. 415, article id 125626Article in journal (Refereed) Published
Abstract [en]

Toxicity of polyethylene (PE) and polyvinyl chloride (PVC) microplastics (MPs), either virgin or spiked with chemicals, was evaluated in two short-lived fish using a freshwater species, zebrafish, and a marine species, marine medaka. Exposures were performed through diet using environmentally relevant concentrations of MPs over 4 months. No modification of classical biomarkers, lipid peroxidation, genotoxicity or F0 behaviour was observed. A significant decrease in growth was reported after at least two months of exposure. This decrease was similar between species, independent from the type of MPs polymer and the presence or not of spiked chemicals, but was much stronger in females. The reproduction was evaluated and it revealed a significant decrease in the reproductive output for both species and in far more serious numbers in medaka. PVC appeared more reprotoxic than PE as were MPs spiked with PFOS and benzophenone-3 compared to MPs spiked with benzo[a]pyrene. Further, PVC-benzophenone-3 produced behavioural disruption in offspring larvae. These results obtained with two species representing different aquatic environments suggest that microplastics exert toxic effects, slightly different according to polymers and the presence or not of sorbed chemicals, which may lead in all cases to serious ecological disruptions.

Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
Adsorbed chemicals, Growth alteration, Polyethylene, Polyvinyl chloride, Reproductive toxicity
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-90622 (URN)10.1016/j.jhazmat.2021.125626 (DOI)000657737000003 ()33740727 (PubMedID)2-s2.0-85102562731 (Scopus ID)
Funder
Swedish Research Council Formas, 2015-01865
Note

Funding Agencies:

French National Research Agency (ANR) ANR-15-JOCE-0002-05

University of Bordeaux, France  

JPI Oceans 

Available from: 2021-03-22 Created: 2021-03-22 Last updated: 2021-06-18Bibliographically approved
Islam, N., Garcia da Fonseca, T., Vilke, J., Gonçalves, J. M., Pedro, P., Keiter, S., . . . Bebianno, M. J. (2021). Perfluorooctane sulfonic acid (PFOS) adsorbed to polyethylene microplastics: Accumulation and ecotoxicological effects in the clam Scrobicularia plana. Marine Environmental Research, 164, Article ID 105249.
Open this publication in new window or tab >>Perfluorooctane sulfonic acid (PFOS) adsorbed to polyethylene microplastics: Accumulation and ecotoxicological effects in the clam Scrobicularia plana
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2021 (English)In: Marine Environmental Research, ISSN 0141-1136, E-ISSN 1879-0291, Vol. 164, article id 105249Article in journal (Refereed) Published
Abstract [en]

Microplastics are widespread in the marine environment, whereby the uptake of these tiny particles by organisms, can cause adverse biological responses. Plastic debris also act as a vector of many contaminants, herein depending on type, size, shape and chemical properties, possibly intensifying their effects on marine organisms. This study aimed to assess the accumulation and potential toxicity of different sizes of microplastics with and without adsorbed perfluorooctane sulfonic acid (PFOS) in the clam Scrobicularia plana. Clams were exposed to low-density polyethylene microplastics (1 mg L-1) of two different sizes (4-6 and 20-25 μm) virgin and contaminated with PFOS (55.7 ± 5.3 and 46.1 ± 2.9 μg g-1 respectively) over 14 days. Microplastic ingestion, PFOS accumulation and filtration rate were determined along with a multi biomarker approach to assess the biological effects of microplastics ingestion. Biomarkers include oxidative stress (superoxide dismutase, catalase, glutathione peroxidases), biotransformation enzymes (glutathione-S-transferases activity), neurotoxicity (acetylcholinesterase activity), oxidative damage and apoptosis. Microplastics ingestion and PFOS accumulation was microplastic size dependent but not PFOS dependent and filtration rate was reduced at the end of the exposure. Reactive oxygen species in gills and digestive gland were generated as a result of exposure to both types of microplastics, confirming the disturbance of the antioxidant system. Larger virgin microparticles lead to stronger impacts, when compared to smaller ones which was also supported by the Integrated Biomarker Responses index calculated for both tissues. An anti-apoptotic response was detected in digestive glands under exposure to any of the MPs treatments. 

Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
Biomarkers, Microplastics, Perfluorooctane sulfonic acid, Scrobicularia plana, Toxicity
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:oru:diva-88939 (URN)10.1016/j.marenvres.2020.105249 (DOI)000618531300002 ()33477023 (PubMedID)2-s2.0-85099631630 (Scopus ID)
Note

Funding Agencies:

EPHEMARE project JPIOCEANS/0005/2015

JPI Oceans  

EMERGEMIX project PTDC/BIA-BMA/30922/2017

Portuguese Foundation for Science and Technology

European Commission UIDB 50006/2020 IF/01616/2015 UID/00350/2020

Available from: 2021-01-27 Created: 2021-01-27 Last updated: 2021-03-25Bibliographically approved
Blanc, M., Antczak, P., Cousin, X., Grunau, C., Scherbak, N., Rüegg, J. & Keiter, S. (2021). The insecticide permethrin induces transgenerational behavioral changes linked to transcriptomic and epigenetic alterations in zebrafish (Danio rerio). Science of the Total Environment, 779, Article ID 146404.
Open this publication in new window or tab >>The insecticide permethrin induces transgenerational behavioral changes linked to transcriptomic and epigenetic alterations in zebrafish (Danio rerio)
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2021 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 779, article id 146404Article in journal (Refereed) Published
Abstract [en]

The pyrethroid insecticide permethrin is widely used for agricultural and domestic purposes. Previous data indicated that it acts as a developmental neurotoxicant and can induce transgenerational effects in non-target organisms. However, associated underlying mechanisms remain unclear. The aim of this study was to investigate permethrin-related transgenerational effects in the zebrafish model, and to identify possible molecular mechanisms underlying inheritance. Zebrafish (F0) were exposed to permethrin during early-life (2 h post-fertilization up to 28 days). The F1 and F2 offspring generations were obtained by pairing exposed F0 males and females, and were bred unexposed. Locomotor and anxiety behavior were investigated, together with transcriptomic and epigenomic (DNA methylation) changes in brains. Permethrin exposed F0 fish were hypoactive at adulthood, while males from the F1 and F2 generations showed a specific decrease in anxiety-like behavior. In F0, transcriptomic data showed enrichment in pathways related to glutamatergic synapse activity, which may partly underlie the behavioral effects. In F1 and F2 males, dysregulation of similar pathways was observed, including a subset of differentially methylated regions that were inherited from the F0 to the F2 generation and indicated stable dysregulation of glutamatergic signaling. Altogether, the present results provide novel evidence on the transgenerational neurotoxic effects of permethrin, as well as mechanistic insight: a transient exposure induces persistent transcriptional and DNA methylation changes that may translate into transgenerational alteration of glutamatergic signaling and, thus, into behavioral alterations.

Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
DNA methylation, Developmental neurotoxicity, Glutamatergic signaling, Pesticide, Transgenerational epigenetic inheritance
National Category
Developmental Biology
Identifiers
urn:nbn:se:oru:diva-90673 (URN)10.1016/j.scitotenv.2021.146404 (DOI)000655687200016 ()33752003 (PubMedID)2-s2.0-85102882062 (Scopus ID)
Funder
Knowledge FoundationHelge Ax:son Johnsons stiftelse
Note

Funding Agency:

French National Research Agency (ANR) ANR-10-LABX-04-01

Available from: 2021-03-23 Created: 2021-03-23 Last updated: 2021-06-18Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0002-2356-6686

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