oru.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Kärrman, Anna
Alternative names
Publications (10 of 62) Show all publications
Stubleski, J., Kukucka, P., Salihovic, S., Lind, P. M., Lind, L. & Kärrman, A. (2018). A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction. Journal of Chromatography A, 1546, 18-27
Open this publication in new window or tab >>A method for analysis of marker persistent organic pollutants in low-volume plasma and serum samples using 96-well plate solid phase extraction
Show others...
2018 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1546, p. 18-27Article in journal (Refereed) Published
Abstract [en]

The objective of this study was to develop and validate a 96-well plate solid phase extraction method for analysis of 23 lipophilic persistent organic pollutants (POPs) in low-volume plasma and serum samples which is applicable for biomonitoring and epidemiological studies. The analysis of selected markers for internal exposure: 16 polychlorinated biphenyls (PCBs), 5 organochlorine pesticides (OCPs), octachlorinated dibenzo-p-dioxin (OCDD), and polybrominated diphenylether 47 (BDE 47) was evaluated by comparing two SPE sorbents and GC-HRMS or GC-MS/MS detection. The final method extracted 23 POPs from 150 μL of serum and plasma using a 96-well extraction plate containing 60 mg Oasis HLB sorbent per well prior to GC-HRMS magnetic sector analysis. The extraction method was applied to 40 plasma samples collected for an epidemiological study. The recovery of selected POPs ranged from 31% to 63% (n = 48), and detection limits ranged from 2.2 to 45 pg/mL for PCBs, 4.2 to 167 pg/mL for OCPs, 7.8 pg/mL for OCDD and 6.1 pg/mL for BDE 47. This method showed good precision with relative standard deviations of selected POP concentrations in quality control samples (n = 48) ranging from 11% to 25%. The trueness was determined with standard reference material serum (n = 48) and the deviation from certified values ranged from 1 to 27%. Of the 23 POPs analyzed, 18 were detected in 43% to 100% of plasma samples collected for the epidemiological study. The method showed good robustness with low inter-well plate variation (11-31%) determined by twelve 96-well plate extractions, and can extract 96 samples, including quality controls and procedural blanks in 2-3 days. Comparison with GC-MS/MS analysis showed that similar concentrations (within 0.5% to 30%) of most POPs could be obtained with GC-APCI-MS/MS. Larger deviations were observed for PCB 194 (60%) and trans-nonachlor (43%). The developed method produces accurate concentrations of low-level marker POPs in plasma and serum, providing a suitable high-throughput sample preparation procedure for biomonitoring and epidemiological studies involving large sample size and limited sample volume. GC-HRMS was chosen over GC-MS/MS, however the latter showed promising results, and could be used as an alternative to GC-HRMS analysis for most POPs.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
High-throughput SPE, Persistent organic pollutants, GC-HRMS, APCI-MS/MS
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:oru:diva-66713 (URN)10.1016/j.chroma.2018.02.057 (DOI)000430766800003 ()29510870 (PubMedID)2-s2.0-85042642069 (Scopus ID)
Funder
Swedish Research Council Formas, 216-2013-478
Note

Funding Agency:

RECETOX Research Infrastructure  LM2015051  CZ.02.1.01/0.0/0.0/16_013/0001761

Available from: 2018-04-23 Created: 2018-04-23 Last updated: 2018-05-14Bibliographically approved
Salihovic, S., Stubleski, J., Kärrman, A., Larsson, A., Fall, T., Lind, L. & Lind, P. M. (2018). Changes in markers of liver function in relation to changes in perfluoroalkyl substances: A longitudinal study. Environment International, 117, 196-203
Open this publication in new window or tab >>Changes in markers of liver function in relation to changes in perfluoroalkyl substances: A longitudinal study
Show others...
2018 (English)In: Environment International, ISSN 0160-4120, E-ISSN 1873-6750, Vol. 117, p. 196-203Article in journal (Refereed) Published
Abstract [en]

Background: While it is known that perfluoroalkyl substances (PFASs) induce liver toxicity in experimental studies, the evidence of an association in humans is inconsistent.

Objective: The main aim of the present study was to examine the association of PFAS concentrations and markers of liver function using panel data.

Methods: We investigated 1002 individuals from Sweden (50% women) at ages 70, 75 and 80 in 2001-2014. Eight PFASs were measured in plasma using isotope dilution ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS). Bilirubin and hepatic enzymes alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma-glutamyltransferase (GGT) were determined in serum using an immunoassay methodology. Mixed-effects linear regression models were used to examine the relationship between the changes in markers of liver function and changes in PFAS levels.

Results: The changes in majority of PFAS concentrations were positively associated with the changes in activity of ALT, ALP, and GGT and inversely associated with the changes in circulating bilirubin after adjustment for gender and the time-updated covariates LDL- and HDL-cholesterol, serum triglycerides, BMI, statin use, smoking, fasting glucose levels and correction for multiple testing. For example, changes in perfluorononanoic acid (PFNA) were associated with the changes liver function markers beta(BILIRUBIN) = -1.56, 95% confidence interval (CI) -1.93 to -1.19, beta(ALT)= 0.04, 95% CI 0.03-0.06, and beta(ALP)= 0.11, 95% CI 0.06-0.15.

Conclusion: Our longitudinal assessment established associations between changes in markers of liver function and changes in plasma PFAS concentrations. These findings suggest a relationship between low-dose background PFAS exposure and altered liver function in the general population.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Epidemiology, Liver function markers, PFAS, ALT, Bilirubin, PFNA
National Category
Occupational Health and Environmental Health
Identifiers
urn:nbn:se:oru:diva-67003 (URN)10.1016/j.envint.2018.04.052 (DOI)000436573400023 ()29754000 (PubMedID)2-s2.0-85046747241 (Scopus ID)
Funder
Swedish Research Council Formas, 2007-2047 2013-478 2015-756
Available from: 2018-05-24 Created: 2018-05-24 Last updated: 2018-07-27Bibliographically approved
Lind, P. M., Salihovic, S., Stubleski, J., Kärrman, A. & Lind, L. (2018). Changes in plasma levels of perfluoroalkyl substances (PFASs) are related to increase in carotid intima-media thickness over 10 years - a longitudinal study. Environmental health, 17, Article ID 59.
Open this publication in new window or tab >>Changes in plasma levels of perfluoroalkyl substances (PFASs) are related to increase in carotid intima-media thickness over 10 years - a longitudinal study
Show others...
2018 (English)In: Environmental health, ISSN 1476-069X, E-ISSN 1476-069X, Vol. 17, article id 59Article in journal (Refereed) Published
Abstract [en]

Background: It has previously been reported that the environmental contaminants perfluoroalkyl substances (PFASs) are linked to atherosclerosis in cross-sectional studies. Since cross-sectional studies could be subject to reverse causation, the purpose of this study was to analyze if the longitudinal changes in PFASs during a 10-year follow-up were related to the change in carotid artery intima-media thickness (IMT, ultrasound) during the same period.

Methods: In the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study, 1016 individuals were investigated at age 70; 826 of them were reinvestigated at age 75 and 602 at age 80 years. Eight different PFASs were measured in plasma by ultra-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and IMT was measured at all three time points. Random-effects mixed regression models were used to examine the associations over time.

Results: IMT increased 0.058 mm during the 10-year period (p <0.0001). Following adjustment for baseline values of PFASs (age 70) and sex, the changes in plasma levels of 6 of the 8 measured PFASs were significantly related to the change in IMT over the 10-year follow-up period in a positive fashion (p <0.0062 using Bonferroni correction for 8 tests). Further adjustment for traditional cardiovascular (CV) risk factors (HDL and LDL cholesterol, smoking, systolic blood pressure, statin use, fasting glucose and serum triglycerides) affected these relationships only marginally.

Conclusion: The change in plasma levels of several PFASs during 10 years was positively related to increase in IMT seen during the same period, giving prospective evidence that PFASs might interfere with the atherosclerotic process.

Place, publisher, year, edition, pages
BMC, 2018
Keywords
Atherosclerosis, Longitudinal, Perfluoroalkyl substances (PFASs), Elderly, Epidemiology, IMT
National Category
Environmental Sciences
Identifiers
urn:nbn:se:oru:diva-68134 (URN)10.1186/s12940-018-0403-0 (DOI)000437299700002 ()29970113 (PubMedID)2-s2.0-85049455829 (Scopus ID)
Funder
Swedish Research Council Formas, 2007-2047
Available from: 2018-07-26 Created: 2018-07-26 Last updated: 2018-07-26Bibliographically approved
Salihovic, S., Fall, T., Ganna, A., Broeckling, C. D., Prenni, J. E., Hyötyläinen, T., . . . Lind, L. (2018). Identification of metabolic profiles associated with human exposure to perfluoroalkyl substances. Journal of Exposure Science and Environmental Epidemiology
Open this publication in new window or tab >>Identification of metabolic profiles associated with human exposure to perfluoroalkyl substances
Show others...
2018 (English)In: Journal of Exposure Science and Environmental Epidemiology, ISSN 1559-0631, E-ISSN 1559-064XArticle in journal (Refereed) Epub ahead of print
Abstract [en]

Recent epidemiological studies suggest that human exposure to perfluoroalkyl substances (PFASs) may be associated with type 2 diabetes and other metabolic phenotypes. To gain further insights regarding PFASs exposure in humans, we here aimed to characterize the associations between different PFASs and the metabolome. In this cross-sectional study, we investigated 965 individuals from Sweden (all aged 70 years, 50% women) sampled in 2001-2004. PFASs were analyzed in plasma using isotope-dilution ultra-pressure liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Non-target metabolomics profiling was performed in plasma using UPLC coupled to time-of-flight mass spectrometry (UPLC-QTOFMS) operated in positive electrospray mode. Multivariate linear regression analysis was used to investigate associations between circulating levels of PFASs and metabolites. In total, 15 metabolites, predominantly from lipid pathways, were associated with levels of PFASs following adjustment for sex, smoking, exercise habits, education, energy, and alcohol intake, after correction for multiple testing. Perfluorononanoic acid (PFNA) and perfluoroundecanoic acid (PFUnDA) were strongly associated with multiple glycerophosphocholines and fatty acids including docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA). We also found that the different PFASs evaluated were associated with distinctive metabolic profiles, suggesting potentially different biochemical pathways in humans.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
Keywords
Epidemiology, Metabolomics, PFOA, PFOS, Perfluoroalkyl substances, XCMS
National Category
Occupational Health and Environmental Health Public Health, Global Health, Social Medicine and Epidemiology
Identifiers
urn:nbn:se:oru:diva-68801 (URN)10.1038/s41370-018-0060-y (DOI)30185940 (PubMedID)
Available from: 2018-09-10 Created: 2018-09-10 Last updated: 2018-09-11Bibliographically approved
Stubleski, J., Lind, L., Salihovic, S., Lind, P. M. & Kärrman, A. (2018). Longitudinal changes in persistent organic pollutants (POPs) from 2001 to 2009 in a sample of elderly Swedish men and women. Environmental Research, 165, 193-200
Open this publication in new window or tab >>Longitudinal changes in persistent organic pollutants (POPs) from 2001 to 2009 in a sample of elderly Swedish men and women
Show others...
2018 (English)In: Environmental Research, ISSN 0013-9351, E-ISSN 1096-0953, Vol. 165, p. 193-200Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Prospective cohort studies evaluating the temporal trends of background-level persistent organic pollutants (POPs) and their potential negative health effects in humans are needed.

OBJECTIVE: The objectives of this study are to examine the five year longitudinal trend in chlorinated and brominated (Cl/Br) POP concentrations in a sample of elderly individuals and to investigate the relationship between gender, changes in body weight, plasma lipid levels and POP concentrations.

METHODS: In the population-based Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study, plasma samples were collected from the same individuals over a 5 year period. Originally 992 subjects (all aged 70) were sampled between 2001 and 2004 and 814 returning subjects (all aged 75) were sampled again from 2006 to 2009. Plasma concentrations of 16 polychlorinated biphenyls (PCBs), 5 organochlorine pesticides (OCPs), octachlorinated dibenzo-p-dioxin (OCDD), and one polybrominated diphenylether (BDE 47) were determined using high-throughput 96-well plate solid phase extraction and gas chromatography-high resolution mass spectrometry (GC-HRMS).

RESULTS: During the 5-year follow-up, plasma concentrations of all POPs significantly decreased (p < 0.00001). Median reductions ranged from 4% (PCB105) to 45% (PCB 99), with most reductions being in the 30-40% range. For most POPs, a larger decline was seen in men than in women. The relationship between the weight change and change in POP concentrations was generally negative, but a positive relationship between lipid levels and POP concentrations when expressed as wet-weight was observed. In general, similar changes in POP concentrations and their relationships to body weight were observed regardless of using either wet-weight (pg/mL) or lipid-normalized (ng/g lipid) concentrations.

CONCLUSION: In this longitudinal cohort study, gender and minor, but varying changes in body weight and lipid levels greatly influenced the individual-based changes in POP concentrations. In general, our findings suggest that men and women with larger decreases in body weight and greater increases in lipid levels have the slowest decline in body burden of POPs. Based on the results from this study, either wet-weight or lipid normalized concentrations can be used to determine the percent change in POP concentrations and their relationships to physiological changes and differences.

Place, publisher, year, edition, pages
Academic Press, 2018
Keywords
Persistent organic pollutants, Longitudinal trend, PIVUS cohort, Body weight, Plasma lipids
National Category
Occupational Health and Environmental Health
Identifiers
urn:nbn:se:oru:diva-66867 (URN)10.1016/j.envres.2018.04.009 (DOI)000437551200021 ()29715601 (PubMedID)2-s2.0-85046341057 (Scopus ID)
Funder
Swedish Research Council, K2009-64X-21031-01-3Swedish Research Council Formas, 216-2007-2047
Available from: 2018-05-21 Created: 2018-05-21 Last updated: 2018-07-25Bibliographically approved
Eriksson, U., Haglund, P. & Kärrman, A. (2017). Contribution of precursor compounds to the release of per- and polyfluoroalkyl substances (PFASs) from waste water treatment plants (WWTPs). Journal of Environmental Sciences(China), 61, 80-90
Open this publication in new window or tab >>Contribution of precursor compounds to the release of per- and polyfluoroalkyl substances (PFASs) from waste water treatment plants (WWTPs)
2017 (English)In: Journal of Environmental Sciences(China), ISSN 1001-0742, E-ISSN 1878-7320, Vol. 61, p. 80-90Article in journal (Refereed) Published
Abstract [en]

Per- and polyfluoroalkyl substances (PFASs) are ubiquitous in sludge and water from waste water treatment plants, as a result of their incorporation in everyday products and industrial processes. In this study, we measured several classes of persistent PFASs, precursors, transformation intermediates, and newly identified PFASs in influent and effluent sewage water and sludge from three municipal waste water treatment plants in Sweden, sampled in 2015. For sludge, samples from 2012 and 2014 were analyzed as well. Levels of precursors in sludge exceeded those of perfluoroalkyl acids and sulfonic acids (PFCAs and PFSAs), in 2015 the sum of polyfluoroalkyl phosphoric acid esters (PAPs) were 15-20ng/g dry weight, the sum of fluorotelomer sulfonic acids (FTSAs) was 0.8-1.3ng/g, and the sum of perfluorooctane sulfonamides and ethanols ranged from non-detected to 3.2ng/g. Persistent PFSAs and PFCAs were detected at 1.9-3.9ng/g and 2.4-7.3ng/g dry weight, respectively. The influence of precursor compounds was further demonstrated by an observed substantial increase for a majority of the persistent PFCAs and PFSAs in water after waste water treatment. Perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA), perfluorohexane sulfonic acid (PFHxS), and perfluorooctane sulfonic acid (PFOS) had a net mass increase in all WWTPs, with mean values of 83%, 28%, 37% and 58%, respectively. The load of precursors and intermediates in influent water and sludge combined with net mass increase support the hypothesis that degradation of precursor compounds is a significant contributor to PFAS contamination in the environment.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
diPAPs; FTSAs; Waste water; Daily discharge; PFCA precursor
National Category
Occupational Health and Environmental Health
Identifiers
urn:nbn:se:oru:diva-62948 (URN)10.1016/j.jes.2017.05.004 (DOI)000416721600010 ()29191318 (PubMedID)2-s2.0-85019583169 (Scopus ID)
Funder
Swedish Environmental Protection Agency
Available from: 2017-12-07 Created: 2017-12-07 Last updated: 2018-01-13Bibliographically approved
Blanc, M., Kärrman, A., Kukučka, P., Scherbak, N. & Keiter, S. (2017). Mixture-specific gene expression in zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonic acid (PFOS), perfluorohexanoic acid (PFHxA) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126). Science of the Total Environment, 590-591, 249-257
Open this publication in new window or tab >>Mixture-specific gene expression in zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonic acid (PFOS), perfluorohexanoic acid (PFHxA) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126)
Show others...
2017 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 590-591, p. 249-257Article in journal (Refereed) Published
Abstract [en]

Perfluorooctane sulfonic acid (PFOS) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) are persistent organic pollutants of high concern because of their environmental persistence, bioaccumulation and toxic properties. Besides, the amphiphilic properties of fluorinated compounds such as PFOS and perfluorohexanoic acid (PFHxA) suggest a role in increasing cell membrane permeability and solubilizing chemicals. The present study aimed at investigating whether PFOS and PFHxA are capable of modifying the activation of PCB126 toxicity-related pathways. For this purpose, zebrafish embryos were exposed in semi-static conditions to 7.5 μg/L of PCB126 alone, in the presence of 25 mg/L of PFOS, 15.7 mg/L of PFHxA or in the presence of both PFOS and PFHxA. Quantitative PCR was performed on embryos aged from 24 h post fertilization (hpf) to 96 hpf to investigate expression changes of genes involved in metabolism of xenobiotics (ahr2, cyp1a), oxidative stress (gpx1a, tp53), lipids metabolism (acaa2, osbpl1a), and epigenetic mechanisms (dnmt1, dnmt3ba). Cyp1a and ahr2 expression were significantly induced by the presence of PCB126. However, after 72 and 78 h of exposure, induction of cyp1a expression was significantly lower when embryos were co-exposed to PCB126 + PFOS + PFHxA when compared to PCB126-exposed embryos. Significant upregulation of gpx1a occurred after exposure to PCB126 + PFHxA and to PCB126 + PFOS + PFHxA at 30 and 48 hpf. Besides, embryos appeared more sensitive to PCB126 + PFOS + PFHxA at 78 hpf: acaa2 and osbpl1a were significantly downregulated; dnmt1 was significantly upregulated. While presented as environmentally safe, PFHxA demonstrated that it could affect gene expression patterns in zebrafish embryos when combined to PFOS and PCB126, suggesting that such mixture may increase PCB126 toxicity. This is of particular relevance since PFHxA is persistent and still being ejected into the environment. Moreover, it provides additional information as to the importance to integrate mixture effects of chemicals in risk assessment and biomonitoring frameworks.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Mixture toxicity PFAS Molecular pathway Zebrafish cyp1a Lipids
National Category
Environmental Sciences Biochemistry and Molecular Biology
Research subject
Enviromental Science
Identifiers
urn:nbn:se:oru:diva-57792 (URN)10.1016/j.scitotenv.2017.02.232 (DOI)000399511800026 ()28283292 (PubMedID)2-s2.0-85014558344 (Scopus ID)
Note

Funding agencies:

ERASMUS program 

Available from: 2017-05-22 Created: 2017-05-22 Last updated: 2017-10-18Bibliographically approved
Bjurlid, F., Kärrman, A., Ricklund, N. & Hagberg, J. (2017). Occurrence of brominated dioxins in a study using various firefighting methods. Science of the Total Environment, 599-600, 1213-1221
Open this publication in new window or tab >>Occurrence of brominated dioxins in a study using various firefighting methods
2017 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 599-600, p. 1213-1221Article in journal (Refereed) Published
Abstract [en]

The use of different firefighting methods influences how fast a fire is extinguished and how fast the temperature drops in the area affected by the fire. These differences may also influence the formation of harmful pollutants during firefighting of an accidental fire. The aim was to study occurrence of brominated dibenzo-p-dioxins and furans (PBDD/Fs) in gas and soot during five fire scenarios resembling a small apartment fire and where different firefighting methods were used. Samples of gas and soot were taken both during the buildup of the fire and during the subsequent extinguishing of the fire while using different firefighting methods (nozzle, compressed air foam system, cutting extinguisher) and an extinguishing additive. New containers equipped with identical sets of combustible material were used for the five tests. The use of different firefighting methods and extinguishing additive induced variations in concentration and congener profiles of detected PBDD/Fs. The concentration range of Sigma PBDD/Fs in gas was 4020-18,700 pg/m(3), and in soot 76-4092 pg/m(2). PBDFs were the predominant congeners and 1,2,3,4,6,7,8-HpBDF was the most abundant congener. Chlorinated dibenzo-p-dioxins and furans (PCDD/Fs) were also monitored. The PBDD/Fs contributed with in average 97% to the total (PCDD/Fs plus PBDD/Fs) toxic equivalents, in soot and gas. During extinguishing, the shorter time the temperature was around 300 degrees C, the lower occurrence of PBDD/Fs. In the study the firefighting methods showed a difference in how effectively they induced a temperature decrease below 300 degrees C in the fire zone during quenching, where cutting extinguishing using additive and the compressed air foam system showed the fastest drop in temperature.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
PBDD/F, PCDD/F, Fire, Cutting extinguisher, Foam, Additive, Nozzle, Formation
National Category
Environmental Sciences
Research subject
Enviromental Science
Identifiers
urn:nbn:se:oru:diva-59123 (URN)10.1016/j.scitotenv.2017.05.087 (DOI)000405253500018 ()28514839 (PubMedID)2-s2.0-85019068316 (Scopus ID)
Funder
Swedish Civil Contingencies Agency
Available from: 2017-08-22 Created: 2017-08-22 Last updated: 2018-09-04Bibliographically approved
Eriksson, U., Mueller, J. F., Toms, L.-M. L., Hobson, P. & Kärrman, A. (2017). Temporal trends of PFSAs, PFCAs and selected precursors in Australian serum from 2002 to 2013. Environmental Pollution, 220(Pt A), 168-177
Open this publication in new window or tab >>Temporal trends of PFSAs, PFCAs and selected precursors in Australian serum from 2002 to 2013
Show others...
2017 (English)In: Environmental Pollution, ISSN 0269-7491, E-ISSN 1873-6424, Vol. 220, no Pt A, p. 168-177Article in journal (Refereed) Published
Abstract [en]

Per- and polyfluoroalkyl substances (PFASs) are a family of compounds that includes numerous compound classes. To date, only a subset of these PFASs have been studied thoroughly in the general population. In this study, pooled serum samples from Australia collected in 2002-2013 were analyzed for PFASs according to gender and age (age categories of 0-4 years, 5-15 years, 16-30 years, 31-45 years, 46-60 years, and >60 years), in total 54 pooled samples and 4920 individuals. Compound classes included were perfluorocarboxylic acids (PFCAs), perfluorosulfonic acids (PFSAs), and two groups of PFCA precursor compounds; polyfluoroalkyl phosphate diesters (diPAPs), and fluorotelomer sulfonic acids (FTSAs). Several PFASs that were not reported in previous studies of Australian serum samples were found in this sample set including; diPAPs, FTSAs, perfluoropentane sulfonic acid (PFPeS), perfluoroheptane sulfonic acid (PFHpS), perfluoroheptane carboxylic acid (PFHpA), perfluoroundecanoic acid (PFUnDA), perfluorododecanoic acid (PFDoDA), and perfluorotridecanoic acid (PFTrDA). Various temporal trends were observed with a significant reduction (p < 0.05) between 2002 and 2013 for 8:2 FTSA, perflurohexane sulfonic acid (PFHxS), PFHpS, PFOS, and perflurooctanoic acid (PFOA). Levels of longer-chained PFDA and PFUnDA started to decrease more recently, between 2006 and 2013, while PFDoDA increased during the same time period. Higher levels in younger age groups (0-4 and 5-15 years) compared to adults (>15 years) were found for 8:2 FTSA and PFHpA, while levels of PFHpS, PFOS, PFUnDA, PFDoDA and PFTrDA were higher in adult age groups compared to younger age groups. Gender-specific patterns were seen for PFOA, PFHxS, PFHpS and PFOS, where levels were lower in women. Changes in manufacturing processes were reflected in the temporal time trends, and differences in bioaccumulation potential between homologues could be associated with age trends. Our results emphasize the importance of including emerging classes of PFASs in biomonitoring studies.

Place, publisher, year, edition, pages
Oxford, United Kingdom: Elsevier, 2017
Keywords
diPaPs, FTSAs, serum, temporal trend, PFAS
National Category
Occupational Health and Environmental Health
Identifiers
urn:nbn:se:oru:diva-54422 (URN)10.1016/j.envpol.2016.09.036 (DOI)000390736700019 ()27726977 (PubMedID)2-s2.0-84992359649 (Scopus ID)
Note

Funding Agencies:

ARC future fellowship FF120100546

Queensland Health

Available from: 2017-01-11 Created: 2017-01-10 Last updated: 2018-07-30Bibliographically approved
Lind, M., Salihovic, S., Stubleski, J., Kärrman, A., van Bavel, B. & Lind, L. (2017). The changes in plasma levels of perfluoroalkyl substances (PFASs) are related to the increase in carotid intima-media thickness over 10 years. Paper presented at 85th Congress of the European-Atherosclerosis-Society (EAS), Prague, Czech Republic, April 23-26, 2017. Atherosclerosis, 263, E18-E18
Open this publication in new window or tab >>The changes in plasma levels of perfluoroalkyl substances (PFASs) are related to the increase in carotid intima-media thickness over 10 years
Show others...
2017 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 263, p. E18-E18Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Aim: It has previously been reported that the environmental contaminants perfluoroalkyl substances (PFASs) are linked to atherosclerosis in cross-sectional studies. Since cross-sectional studies could be subject to reverse causation, we here analyzed if the longitudinal changes in PFASs during a 10 years follow-up were related to the change in intima-media thickness (IMT) during the same period.

Methods: In the Prospective Investigation of the Vasculature in Uppsala Seniors (PIVUS) study,1,016 individuals were investigated at age 70. 826 of those were reinvestigated at age 75 and 606 at age 80 years. Eight different PFASs and carotid artery intima-media thickness (IMT, ultrasound) were measured at the three time-points.

Results: IMT increased 0.058 mm during the 10-year period (p<0.0001). Following adjustment for baseline values of PFASs (age 70) and sex, the changes in plasma levels of 6 of the 8 measured PFASs were significantly related to the change in IMT over the 10-year follow-up period (p<0.0062 using Bonferroni correction for 8 tests). Further adjustment for traditional CV risk factors (HDL and LDL-cholesterol, smoking, systolic blood pressure, statin use, fasting glucose and serum triglycerides) did only affect these relationships marginally.

Conclusions: The change in plasma levels of several PFASs during 10 years was related the increase in IMT seen during the same period, giving further evidence that PFASs might interfere with the atherosclerotic process.

Place, publisher, year, edition, pages
Elsevier, 2017
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:oru:diva-60725 (URN)10.1016/j.atherosclerosis.2017.06.083 (DOI)000407634000051 ()
Conference
85th Congress of the European-Atherosclerosis-Society (EAS), Prague, Czech Republic, April 23-26, 2017
Available from: 2017-09-11 Created: 2017-09-11 Last updated: 2017-09-11Bibliographically approved
Organisations

Search in DiVA

Show all publications