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Strid, Åke, ProfessorORCID iD iconorcid.org/0000-0003-3315-8835
Publications (10 of 80) Show all publications
Hideg, É. & Strid, Å. (2017). The effects of UV-B on the biochemistry and metabolism of plants. In: Brian R. Jordan (Ed.), UV-B radiation and plant life: molecular biology to ecology (pp. 90-110). Wallingford, UK: CABI Publishing
Open this publication in new window or tab >>The effects of UV-B on the biochemistry and metabolism of plants
2017 (English)In: UV-B radiation and plant life: molecular biology to ecology / [ed] Brian R. Jordan, Wallingford, UK: CABI Publishing, 2017, p. 90-110Chapter in book (Refereed)
Abstract [en]

This chapter focuses on the effects of UV-B radiation on the biochemistry and metabolism of plants and their underlying mechanisms. Information on the UV-inducible metabolites and protection responses of plants against UV-B radiation are also discussed.

Place, publisher, year, edition, pages
Wallingford, UK: CABI Publishing, 2017
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biochemistry; Biology
Identifiers
urn:nbn:se:oru:diva-53555 (URN)10.1079/9781780648590.0090 (DOI)978-1-78064-859-0 (ISBN)978-1-78064-860-6 (ISBN)
Projects
UV4quality
Funder
Knowledge FoundationSwedish Research Council Formas
Note

This chapter is dedicated to Prof. Jan M Anderson (1932-2015) and to her lifetime achievements in photosynthesis and plant biology

Available from: 2016-11-18 Created: 2016-11-18 Last updated: 2018-03-06Bibliographically approved
Kalbina, I., Lagerqvist, N., Moiane, B., Ahlm, C., Andersson, S., Strid, Å. & Falk, K. I. (2016). Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oraladministration of the transgenic plants. Protein Expression and Purification, 127, 61-67
Open this publication in new window or tab >>Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oraladministration of the transgenic plants
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2016 (English)In: Protein Expression and Purification, ISSN 1046-5928, E-ISSN 1096-0279, Vol. 127, p. 61-67Article in journal (Refereed) Published
Abstract [en]

The zoonotic Rift Valley fever virus affects livestock and humans in Africa and on the Arabian Peninsula.The economic impact of this pathogen due to livestock losses, as well as its relevance to public health,underscores the importance of developing effective and easily distributed vaccines. Vaccines that can bedelivered orally are of particular interest.

Here, we report the expression in transformed plants (Arabidopsis thaliana) of Rift Valley fever virusantigens. The antigens used in this study were the N protein and a deletion mutant of the Gn glycoprotein.Transformed lines were analysed for specific mRNA and protein content by RT-PCR and Westernblotting, respectively. Furthermore, the plant-expressed antigens were evaluated for their immunogenicityin mice fed the transgenic plants. After oral intake of fresh transgenic plant material, a proportionof the mice elicited specific IgG antibody responses, as compared to the control animals that were fedwild-type plants and of which none sero-converted.

Thus, we show that transgenic plants can be readily used to express and produce Rift Valley Fever virusproteins, and that the plants are immunogenic when given orally to mice. These are promising findingsand provide a basis for further studies on edible plant vaccines against the Rift Valley fever virus.

Place, publisher, year, edition, pages
San Diego, USA: Elsevier, 2016
Keyword
Antigen production, Arabidopsis thaliana, Rift valley fever virus, Plant vaccine, Transformation
National Category
Immunology in the medical area Immunology Biochemistry and Molecular Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Research subject
Biochemistry; Immunology
Identifiers
urn:nbn:se:oru:diva-51367 (URN)10.1016/j.pep.2016.07.003 (DOI)000382181300009 ()27402440 (PubMedID)2-s2.0-84978634507 (Scopus ID)
Projects
Vaccinutveckling och vaccinproduktion
Funder
Knowledge FoundationStiftelsen Olle Engkvist Byggmästare
Note

Funding Agencies:

Swedish International Development Cooperation Agency (SIDA)

Örebro University's Faculty for Business, Science and Technology

Sparbanksstiftelsen Nya

Available from: 2016-07-17 Created: 2016-07-17 Last updated: 2018-01-10Bibliographically approved
Petra, M., Marija, V., Czégény, G., Veljovic Jovanovic, S., Strid, Å. & Hideg, É. (2016). Evaluation of procedures for assessing anti- and pro-oxidants in plant samples. Analytical Methods, 8(28), 5569-5580
Open this publication in new window or tab >>Evaluation of procedures for assessing anti- and pro-oxidants in plant samples
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2016 (English)In: Analytical Methods, ISSN 1759-9660, E-ISSN 1759-9679, Vol. 8, no 28, p. 5569-5580Article, review/survey (Refereed) Published
Abstract [en]

Plants as well as other aerobic organisms constantly produce reactive oxygen species (ROS). At regulatedlow concentrations ROS may serve as signal molecules, while in excessive amounts these may causeoxidative damage to biomolecules. Actual cellular concentrations are controlled by a network of variousantioxidants, and acclimation to stress conditions is achieved by a dynamic balance of ROS productionand neutralization. Accordingly, plant stress physiology studies generally include an array of methodstesting the occurrence of ROS as well as evaluating antioxidant capacities. The aim of the present workis to provide an overview of these methods, with special emphasis on avoiding errors that can possiblylead to either inaccurate data or misinterpretations of otherwise correct measurements.

Place, publisher, year, edition, pages
Cambridge, United Kingdom: Royal Society of Chemistry, 2016
National Category
Biochemistry and Molecular Biology Food Science
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-51368 (URN)10.1039/c6ay01342b (DOI)000379896600003 ()2-s2.0-84978732075 (Scopus ID)
Projects
Plant UV Photobiology
Note

Funding Agencies:

EU COST action FA0906 'UV4growth'

Ministry of Education and Science, Republic of Serbia III 43010

Hungarian Scientific Grant Agency OTKA NN-85349

Örebro University's Faculty for Business, Science, and Technology

Knowledge Foundation Sweden (project 'UV4quality')

FORMAS research council

Available from: 2016-07-17 Created: 2016-07-17 Last updated: 2017-11-28Bibliographically approved
Hansson, C., Schön, K., Kalbina, I., Strid, Å., Andersson, S., Bokarewa, M. I. & Lycke, N. Y. (2016). Feeding transgenic plants that express a tolerogenic fusion protein effectively protects against arthritis. Plant Biotechnology Journal, 14(4), 1106-1115
Open this publication in new window or tab >>Feeding transgenic plants that express a tolerogenic fusion protein effectively protects against arthritis
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2016 (English)In: Plant Biotechnology Journal, ISSN 1467-7644, E-ISSN 1467-7652, Vol. 14, no 4, p. 1106-1115Article in journal (Refereed) Published
Abstract [en]

Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated if the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants and in this way induce oral tolerance and protect against arthritis. The fusion protein was recombinantly expressed in Arabidopsis thaliana plants, which were fed to H-2q restricted DBA/1 mice to assess the preventive effect on collagen-induced arthritis (CIA). The treatment resulted in fewer mice exhibiting disease and arthritis scores were significantly reduced. Immune suppression was evident in treated mice and serum biomarkers for inflammation as well as anti-collagen IgG responses were reduced. In spleen draining and lymph nodes, CD4+ T cell responses were reduced. Concomitant with a reduced effector T cell activity with lower IFNg, IL-13 and IL-17A production we observed an increase in IL-10 production to recall antigen stimulation in vitro, suggesting reduced Th1, Th2 and Th17 activity subsequent to upregulated IL-10 and regulatory T cell (Treg) functions. The present study shows that edible plants expressing a tolerogen were effective at stimulating CD4 T cell tolerance and in protecting against CIA disease. Our study conveys optimism as to the potential of using edible plants for oral treatment of rheumatoid arthritis.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2016
Keyword
autoimmunity; transgenic plants; edible plants; CIA; IL-10; FoxP3
National Category
Immunology in the medical area Plant Biotechnology Biochemistry and Molecular Biology
Research subject
Biochemistry; Immunology; Molecular Biology
Identifiers
urn:nbn:se:oru:diva-45625 (URN)10.1111/pbi.12479 (DOI)000373069400006 ()26403330 (PubMedID)2-s2.0-84961215734 (Scopus ID)
Funder
Swedish Cancer SocietySwedish Research CouncilKnut and Alice Wallenberg FoundationAFA InsuranceEU, FP7, Seventh Framework ProgrammeSwedish Foundation for Strategic Research
Note

Funding Agency:

UNISEC

Available from: 2015-08-22 Created: 2015-08-22 Last updated: 2018-01-11Bibliographically approved
Hadad, R., Marks, E., Kalbina, I., Schön, K., Unemo, M., Lycke, N., . . . Andersson, S. (2016). Protection against genital tract Chlamydia trachomatis infection following intranasal immunization with a novel recombinant MOMP VS2/4 antigen. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), 124, 1078-1086
Open this publication in new window or tab >>Protection against genital tract Chlamydia trachomatis infection following intranasal immunization with a novel recombinant MOMP VS2/4 antigen
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2016 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 124, p. 1078-1086Article in journal (Refereed) Published
Abstract [en]

The asymptomatic nature of most Chlamydia trachomatis infections and the lack of appropriate effects by current prevention and management call for vaccine development. We evaluated a recombinant subunit vaccine candidate based on the major outer membrane protein variable segments 2 and 4 (MOMP VS2/4). To achieve maximal immunogenicity and ease of production and purification, MOMP VS2/4 was constructed by using highly immunogenic sequences of MOMP only, thereby minimizing the presence of hydrophobic regions, and spacing the immunogenic epitopes with a flexible amino acid sequence. A purification tag was also added. The MOMP VS2/4 was given intranasally, with or without intravaginal boost, with cholera toxin (CT) adjuvant to C57BL/6 mice, which were screened for immunogenicity and protection against a live challenge infection with C. trachomatis serovar D. Bacterial shedding, cell-mediated responses, and antibody responses were monitored. Immunized mice exhibited significantly less bacterial shedding and were better protected against infertility as compared to unimmunized control mice. Immunizations stimulated both systemic and local specific antibody (IgG1, IgG2c, and IgA) responses, and primed T cells that produced interferon-c and interleukins 13 and 17 upon challenge with recall antigen. Thus, MOMP VS2/4, in combination with CT adjuvant, stimulated Th1, Th2, and Th17 effector cells, and generated protective immunity associated with less pathology. We regard MOMP VS2/4 as a promising candidate for further development into a mucosal chlamydial vaccine.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell, 2016
Keyword
Chlamydia trachomatis, vaccine, major outer membrane protein, mice, antibody response, T cells
National Category
Immunology in the medical area Microbiology in the medical area
Research subject
Biochemistry; Immunology; Microbiology; Infectious Diseases
Identifiers
urn:nbn:se:oru:diva-53554 (URN)10.1111/apm.12605 (DOI)000388265700008 ()27859689 (PubMedID)2-s2.0-84995753108 (Scopus ID)
Projects
Utveckling av vacciner mot sexuellt överförbara sjukdomarMolecular farming
Funder
Stiftelsen Olle Engkvist Byggmästare
Note

Funding Agencies:

Sparbanksstiftelsen Nya

Örebro University's Faculty for Business, Science, and Technology

Foundation for Medical Research at Örebro University Hospital

Available from: 2016-11-18 Created: 2016-11-18 Last updated: 2018-01-13Bibliographically approved
Jordan, B. R., Strid, Å. & Wargent, J. J. (2016). What role does UVB play in determining photosynthesis? (3ed.). In: Mohammad Pessarakli (Ed.), Handbook of Photosynthesis: (pp. 275-286). Boca Raton: CRC Press
Open this publication in new window or tab >>What role does UVB play in determining photosynthesis?
2016 (English)In: Handbook of Photosynthesis / [ed] Mohammad Pessarakli, Boca Raton: CRC Press, 2016, 3, p. 275-286Chapter in book (Refereed)
Place, publisher, year, edition, pages
Boca Raton: CRC Press, 2016 Edition: 3
National Category
Other Agricultural Sciences Botany
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-39416 (URN)9781482230734 (ISBN)
Projects
Plant UV Photobiology
Available from: 2014-12-08 Created: 2014-12-08 Last updated: 2018-03-05Bibliographically approved
Morales, L. O., Brosché, M., Vainonen, J. P., Sipari, N., Lindfors, A., Strid, Å. & Aphalo, P. J. (2015). Are solar UV-B- and UV-A-dependent gene expression and metabolite accumulation in Arabidopsis mediated by the stress response regulator RADICAL-INDUCED CELL DEATH1?. Plant, Cell and Environment, 38(5), 878-891
Open this publication in new window or tab >>Are solar UV-B- and UV-A-dependent gene expression and metabolite accumulation in Arabidopsis mediated by the stress response regulator RADICAL-INDUCED CELL DEATH1?
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2015 (English)In: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 38, no 5, p. 878-891Article in journal (Refereed) Published
Abstract [en]

Wavelengths in the ultraviolet (UV) region of the solar spectrum, UV-B (280-315nm) and UV-A (315-400nm), are key environmental signals modifying several aspects of plant physiology. Despite significant advances in the understanding of plant responses to UV-B and the identification of signalling components involved, there is limited information on the molecular mechanisms that control UV-B signalling in plants under natural sunlight. Here, we aimed to corroborate the previous suggested role for RADICAL-INDUCED CELL DEATH1 (RCD1) in UV-B signalling under full spectrum sunlight. Wild-type Arabidopsis thaliana and the rcd1-1 mutant were used in an experimental design outdoors where UV-B and UV-A irradiances were manipulated using plastic films, and gene expression, PYRIDOXINE BIOSYNTHESIS1 (PDX1) accumulation and metabolite profiles were analysed in the leaves. At the level of transcription, RCD1 was not directly involved in the solar UV-B regulation of genes with functions in UV acclimation, hormone signalling and stress-related markers. Furthermore, RCD1 had no role on PDX1 accumulation but modulated the UV-B induction of flavonoid accumulation in leaves of Arabidopsis exposed to solar UV. We conclude that RCD1 does not play an active role in UV-B signalling but rather modulates UV-B responses under full spectrum sunlight.

Keyword
Flavonoids; RCD1; Signalling; Solar UV; Stress; UV acclimation
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-34535 (URN)10.1111/pce.12341 (DOI)000353898400004 ()24689869 (PubMedID)2-s2.0-84927723426 (Scopus ID)
Note

Funding Agencies:

Academy of Finland 116775 135751 140981

Finnish Cultural Foundation

LignoCell - TEKES

LignoCell - Academy of Finland

Available from: 2014-04-01 Created: 2014-04-01 Last updated: 2017-12-05Bibliographically approved
Czégény, G., Wu, M., Dér, A., Eriksson, L. A., Strid, Å. & Hideg, É. (2014). Hydrogen peroxide contributes to the ultraviolet-B (280-315 nm) induced oxidative stress of plant leaves through multiple pathways. FEBS Letters, 588(14), 2255-2261
Open this publication in new window or tab >>Hydrogen peroxide contributes to the ultraviolet-B (280-315 nm) induced oxidative stress of plant leaves through multiple pathways
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2014 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 588, no 14, p. 2255-2261Article in journal (Refereed) Published
Abstract [en]

Solar UV-B (280-315 nm) radiation is a developmental signal in plants but may also cause oxidative stress when combined with other environmental factors. Using computer modelling and in solution experiments we show that UV-B is capable of photosensitizing hydroxyl radical production from hydrogen peroxide. We present evidence that the oxidative effect of UV-B in leaves is at least two-fold: (i) it increases cellular hydrogen peroxide concentrations, to a larger extent in pyridoxine antioxidant mutant pdx1.3-1 Arabidopsis and (ii) is capable of a partial photo-conversion of both ‘natural’ and ‘extra’ hydrogen peroxide to hydroxyl radicals. As stress conditions other than UV can increase cellular hydrogen peroxide levels, synergistic deleterious effects of various stresses may be expected already under ambient solar UV-B.

Keyword
Arabidopsis; Computer modeling; Hydrogen peroxide; Hydroxyl radical; Leaf; Oxygen radicals; Photosynthesis; Plant; Terephthalic acid; UV photobiology
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-34932 (URN)10.1016/j.febslet.2014.05.005 (DOI)000338401100011 ()24846142 (PubMedID)2-s2.0-84902662550 (Scopus ID)
Projects
UV Plant Photobiology
Funder
Knowledge FoundationCarl Tryggers foundation
Note

Funding Agencies:

COST Action FA0906

Hungarian Scientific Grant Agency OTKMNN-85349

Örebro University's Faculty for Business, Science and Technology

Available from: 2014-05-03 Created: 2014-05-03 Last updated: 2017-12-05Bibliographically approved
Lindh, I., Bråve, A., Hallengärd, D., Hadad, R., Kalbina, I., Strid, Å. & Andersson, S. (2014). Oral delivery of plant-derived HIV-1 p24 antigen in low doses shows a superior priming effect in mice compared to high doses. Vaccine, 32(20), 2288-2293
Open this publication in new window or tab >>Oral delivery of plant-derived HIV-1 p24 antigen in low doses shows a superior priming effect in mice compared to high doses
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2014 (English)In: Vaccine, ISSN 0264-410X, E-ISSN 1873-2518, Vol. 32, no 20, p. 2288-2293Article in journal (Refereed) Published
Abstract [en]

During early infection with human immunodeficiency virus type 1 (HIV-1), there is a rapid depletion of CD4+ T-cells in the gut-associated lymphoid tissue (GALT) in the gastrointestinal tract. Therefore, immediate protection at these surfaces is of high priority for the development of an HIV-1 vaccine. Thus, transgenic plants expressing HIV-1 antigens, which are exposed to immune competent cells in the GALT during oral administration, can be interesting as potential vaccine candidates. In the present study, we used two HIV-1 p24 antigen-expressing transgenic plant systems, Arabidopsis thaliana and Daucus carota, in oral immunization experiments. Both transgenic plant systems showed a priming effect in mice and induced humoral immune responses, which could be detected as anti-p24-specific IgG in sera after an intramuscular p24 protein boost. Dose-dependent antigen analyses using transgenic Arabidopsis thaliana indicated that low p24 antigen doses were superior to high p24 antigen doses

Place, publisher, year, edition, pages
Elsevier, 2014
Keyword
Transgenic plants; Arabidopsis thaliana; Carrot; Edible vaccine; Oral immunization; HIV-1 p24
National Category
Immunology in the medical area Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) Biochemistry and Molecular Biology
Research subject
Biochemistry; Immunology
Identifiers
urn:nbn:se:oru:diva-33982 (URN)10.1016/j.vaccine.2014.02.073 (DOI)000335100600005 ()24631072 (PubMedID)2-s2.0-84897502968 (Scopus ID)
Projects
Ätbara vacciner
Funder
Knowledge Foundation
Note

Funding Agencies:

Örebro University's Faculty for Business, Science, and Technology 

Nyckelfonden

Available from: 2014-02-27 Created: 2014-02-27 Last updated: 2018-01-11Bibliographically approved
Wu, M., Strid, Å. & Eriksson, L. A. (2014). Photochemical reaction mechanism of UV-B-induced monomerization of UVR8 dimers as the first signaling event in UV-B-regulated gene expression in plants. Journal of Physical Chemistry B, 118, 951-965
Open this publication in new window or tab >>Photochemical reaction mechanism of UV-B-induced monomerization of UVR8 dimers as the first signaling event in UV-B-regulated gene expression in plants
2014 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 118, p. 951-965Article in journal (Refereed) Published
Abstract [en]

The Arabidopsis thaliana UV RESISTANCE LOCUS8 (UVR8) protein has been identified to specifically mediate photomorphogenic UV-B responses by acting as a UV-B photoreceptor. The dimeric structure of the UVR8 protein dissociates into signaling–active monomers upon UV-B exposure, and the monomers rapidly interact with downstream signaling components to regulate gene expression. UVR8 monomers revert to dimers in the absence of UV-B radiation, thereby reversing transcription activation. UVR8 amino acid residues W233 and W285 have been identified to play critical roles in the UVR8 dimer for the response to UV-B irradiation. In the present work, the photo-reaction mechanism for UVR8 monomerization is explored using quantum chemical cluster calculations, and evaluated by molecular dynamics simulations using the wild type UVR8 dimer and novel force field parameters developed for intermediate radicals formed in the photochemical process. Three different models are investigated, which show that the preferred mechanism for UVR8 monomerization involves electron transfer from residue W233 to W285 and onwards to R338 initiated by UV-B irradiation, coupled to simultaneous proton transfer from W233 to D129 leading to the formation of protonated D129, a deprotonated W233 radical and a neutral R338 radical.. Due to the formation of the neutral R338 radical, salt bridges involving this residue are disrupted together with the concomitant interruption of several other key salt bridges R286-D96, R286-D107, R338-D44, R354-E43 and R354-E53. The resulting large decrease in protein-protein interaction energy arising from this sequence of events lead to the monomerization of the UVR8 dimer. The mechanism presented is in accord with all experimental data available to date.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2014
Keyword
Amino acid residues; Arabidopsis thaliana; Force field parameters; Molecular dynamics simulations; Photochemical process; Protein-protein interactions; Sequence of events; Signaling components
National Category
Physical Chemistry Biochemistry and Molecular Biology
Research subject
Physical Chemistry; Biochemistry
Identifiers
urn:nbn:se:oru:diva-33022 (URN)10.1021/jp4104118 (DOI)000330610400011 ()24410443 (PubMedID)2-s2.0-84893504425 (Scopus ID)
Projects
ULTRAVIOLET RADIATION AS AN ENVIRONMENTAL STIMULI IN BIOLOGICAL SYSTEMS: PERCEPTION, SIGNALLING, MOLECULAR RESPONSES AND MORPHOGENESIS
Funder
Swedish Research CouncilCarl Tryggers foundation
Note

Funding Agency:

Faculty of Science at the University of Gothenburg

Available from: 2014-01-11 Created: 2014-01-11 Last updated: 2017-10-18Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0003-3315-8835

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