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Strid, Åke, ProfessorORCID iD iconorcid.org/0000-0003-3315-8835
Publications (10 of 94) Show all publications
Rai, N., O'Hara, A., Farkas, D., Safronov, O., Ratanasopa, K., Wang, F., . . . Morales, L. O. (2020). The photoreceptor UVR8 mediates the perception of both UV-B and UV-A wavelengths up to 350 nm of sunlight with responsivity moderated by cryptochromes. Plant, Cell and Environment
Open this publication in new window or tab >>The photoreceptor UVR8 mediates the perception of both UV-B and UV-A wavelengths up to 350 nm of sunlight with responsivity moderated by cryptochromes
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2020 (English)In: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040Article in journal (Refereed) Accepted
Abstract [en]

The photoreceptors UV RESISTANCE LOCUS 8 (UVR8) and CRYPTOCHROMES 1 and 2 (CRYs) play major roles in the perception of UV-B (280–315 nm) and UV-A/blue radiation (315–500 nm), respectively. However, it is poorly understood how they function in sunlight. The roles of UVR8 and CRYs were assessed in a factorial experiment with Arabidopsis thaliana wild-type and photoreceptor mutants exposed to sunlight for 6 h or 12 h under five types of filters with cut-offs in UV and blue-light regions. Transcriptome-wide responses triggered by UV-B and UV-A wavelengths shorter than 350 nm (UV-Asw) required UVR8 whereas those induced by blue and UV-A wavelengths longer than 350 nm (UV-Alw) required CRYs. UVR8 modulated gene expression in response to blue light while lack of CRYs drastically enhanced gene expression in response to UV-B and UV-Asw. These results agree with our estimates of photons absorbed by these photoreceptors in sunlight and with in vitro monomerization of UVR8 by wavelengths up to 335 nm. Motif enrichment analysis predicted complex signaling downstream of UVR8 and CRYs. Our results highlight that it is important to use UV waveband definitions specific to plants’ photomorphogenesis as is routinely done in the visible region.

Place, publisher, year, edition, pages
Blackwell Publishing, 2020
Keywords
Arabidopsis thaliana, blue light, cryptochrome, gene expression, photoreceptor interaction, solar radiation, ultraviolet radiation, UVR8
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-79340 (URN)10.1111/pce.13752 (DOI)32167576 (PubMedID)
Funder
Swedish Research Council Formas, 942-2015-516Knowledge Foundation, 20130164Academy of Finland, 252548Academy of Finland, 307335
Available from: 2020-01-22 Created: 2020-01-22 Last updated: 2020-03-25Bibliographically approved
Neugart, S., Hideg, É., Czégény, G., Schreiner, M. & Strid, Å. (2020). Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant. Photochemical and Photobiological Sciences, 19, 217-228
Open this publication in new window or tab >>Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant
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2020 (English)In: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 19, p. 217-228Article in journal (Refereed) Published
Abstract [en]

Pyridoxine (vitamin B6) and its vitamers are used by living organisms both as enzymatic cofactors and as antioxidants. We used Arabidopsis pyridoxine biosynthesis mutant pdx1.3-1to study involvement of the PLP-synthase main polypeptide PDX1 in plant responses to ultraviolet radiation of two different qualities, one containing primarily UV-A (315-400 nm), the other containing both UV-A and UV-B (280-315 nm). The antioxidant capacity and the flavonoid and glucosinolate (GS) profiles were examined. As indicator of stress, F⁠v/F⁠mof photosystem II reaction centers was used. In pdx1.3-1, UV-A+B exposure led to a significant 5% decrease in F⁠v/F⁠mon the last day (day 15), indicating mild stress at this time point. Antioxidant capacity of Col-0 wildtype increased significantly (50-73%) after 1 and 3 days of UV-A+B. Instead, in pdx1.3-1, the antioxidant capacity significantly decreased by 44-52% over the same time period, proving the importance of a full complement of functional PDX1genes for detoxification of reactive oxygen species. There were no significant changes in flavonoid glycoside profile under any light condition. However, the GS profile was significantly altered, both with respect to Arabidopsis accession and exposure to UV. The difference in flavonoid and GS profiles reflect that the GS biosynthesis pathway contains at least one pyridoxine-dependent enzyme, whereas no such enzyme is used in flavonoid biosynthesis. Also, there was strong correlation between the antioxidant capacity and the content of some GS compounds. Our results show that vitamin B6vitamers, functioning both as antioxidants and co-factors, are of importance for physiological fitness of plants.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2020
National Category
Botany Biochemistry and Molecular Biology Analytical Chemistry
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-78129 (URN)10.1039/C9PP00342H (DOI)31961357 (PubMedID)
Projects
UV4quality
Funder
Swedish Research Council Formas, 942-2015-516Knowledge Foundation, 20130164
Available from: 2019-11-25 Created: 2019-11-25 Last updated: 2020-02-19Bibliographically approved
Qian, M., Rosenqvist, E., Flygare, A.-M., Kalbina, I., Teng, Y., Jansen, M. A. K. & Strid, Å. (2020). UV-A light induces a robust and dwarfed phenotype in cucumber plants (Cucumis sativus L.) without affecting fruit yield. Scientia Horticulturae, 263, Article ID 109110.
Open this publication in new window or tab >>UV-A light induces a robust and dwarfed phenotype in cucumber plants (Cucumis sativus L.) without affecting fruit yield
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2020 (English)In: Scientia Horticulturae, ISSN 0304-4238, E-ISSN 1879-1018, Vol. 263, article id 109110Article in journal (Refereed) Published
Abstract [en]

Solar ultraviolet (UV) light influences plant growth and metabolism. Whereas high doses of UV can be deleterious for plants, natural UV doses are important for morphogenesis in many plants species, including those used in horticulture. Greenhouses are widely used for horticultural production and common cladding materials strongly absorb UV. Thus, low amounts of UV may be limiting the optimal development in some plant species. Light supplementation using UV tubes can overcome UV deficiency. Here we study cucumber seedling production in the absence or presence of different UV wavelengths. UV-A- (315-400 nm) and UV-B- (280-315 nm) enriched light was used for exposure and parameters such as the maximum quantum yield of photosystem II, stem development (internode length and diameter, stem dry weight, stem weight per unit of stem length, and stem bending), root biomass, leaf biomass and specific leaf mass were measured. We found that UV-A supplementation resulted in shorter more compact and sturdy plants, properties that are positive from a horticultural perspective. In contrast, UV-B-enriched light led to even smaller plants that lacked the sturdy phenotype. There were no signs of decreased Fv/Fmunder any of the treatments, nor statistically significant differences in fruit yield between the control plants and the UV-treated plants when grown to harvest. In particular, the differences in fruit yield between the controls and the UV-A-treated plants were negligible in all cases. Thus, supplementary UV-A light can be an interesting alternative to chemical growth regulators for production of sturdy horticultural plants.

Place, publisher, year, edition, pages
Elsevier, 2020
Keywords
Cucumber production, Fruit yield, Plant architecture, Plant morphology, Ultraviolet light
National Category
Plant Biotechnology Horticulture Botany Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-78473 (URN)10.1016/j.scienta.2019.109110 (DOI)000508491600018 ()2-s2.0-85076304828 (Scopus ID)
Funder
Knowledge Foundation, 20130164Swedish Research Council Formas, 942-2015-516
Note

Funding Agencies:

Faculty for Business, Science and Technology at Örebro University

Science Foundation Ireland S16/IA/4418

China Scholarship Council 201406320076

Available from: 2019-12-05 Created: 2019-12-05 Last updated: 2020-03-17Bibliographically approved
Rodriguez-Calzada, T., Qian, M., Strid, Å., Neugart, S., Schreiner, M., Torres-Pacheco, I. & Guevara-Gonzales, R. (2019). Effect of UV-B radiation on morphology, phenolic compound production, gene expression, and subsequent drought stress responses in chili pepper (Capsicum annuum L.). Plant physiology and biochemistry (Paris), 134, 94-102
Open this publication in new window or tab >>Effect of UV-B radiation on morphology, phenolic compound production, gene expression, and subsequent drought stress responses in chili pepper (Capsicum annuum L.)
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2019 (English)In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 134, p. 94-102Article in journal (Refereed) Published
Abstract [en]

It has been suggested that accumulation of flavonoids could be a key step in development of plant tolerance to different environmental stresses. Moreover, it has been recognized that abiotic stresses such as drought and UV-B radiation (280-315 nm) induce phenolic compound accumulation, suggesting a role for these compounds in drought tolerance. The aim of the present study was to evaluate the effect of UV-B exposure on chili pepper (Capsicum annuum, cv. ‘Coronel’) plant performance, phenolic compound production, and gene expression associated with response to subsequent drought stress. Additionally, the phenotypic response to drought stress of these plants was studied. UV-B induced a reduction both in stem length, stem dry weight and number of floral primordia. The largest reduction in these variables was observed when combining UV-B and drought. UV-B-treated well-watered plants displayed fructification approximately 1 week earlier than non-UV-B-treated controls. Flavonoids measured epidermally in leaves significantly increased during UV-B treatment. Specifically, UV-B radiation significantly increased chlorogenic acid and apigenin 8-C-hexoside levels in leaves and a synergistic increase of luteolin 6-C-pentoside-8-C-hexoside was obtained by UV-B and subsequent drought stress. Gene expression of phenylalanine ammonia lyase (PAL) and chalcone synthase (CHS) genes also increased during UV-B treatments. On the other hand, expression of genes related to an oxidative response, such as mitochondrial Mn-superoxide dismutase (Mn-SOD) and peroxidase (POD) was not induced by UV-B. Drought stress in UV-B-treated plants induced mitochondrial Mn-SOD gene expression. Taken together, the UV-B treatment did not induce significant tolerance in plants towards drought stress under the conditions used.

Place, publisher, year, edition, pages
Paris, France: Elsevier, 2019
Keywords
Drought, Dualex, Flavonoids, Jalapeno pepper, Morphology, Ultraviolet-B radiation
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-67300 (URN)10.1016/j.plaphy.2018.06.025 (DOI)000455692000010 ()29950274 (PubMedID)2-s2.0-85048888625 (Scopus ID)
Funder
Swedish Research Council Formas, 942 - 2015-516Knowledge Foundation, 20130164
Note

Funding Agencies:

CONACYT  283259

Faculty for Business, Science and Technology at Örebro University

Available from: 2018-06-19 Created: 2018-06-19 Last updated: 2019-01-29Bibliographically approved
Ni, J., Zhao, Y., Tao, R., Yin, L., Gao, L., Strid, Å., . . . Bai, S. (2019). Ethylene mediates the branching of the jasmonate-induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits. Plant Biotechnology Journal
Open this publication in new window or tab >>Ethylene mediates the branching of the jasmonate-induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits
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2019 (English)In: Plant Biotechnology Journal, ISSN 1467-7644, E-ISSN 1467-7652Article in journal (Refereed) Epub ahead of print
Abstract [en]

Flavonoid accumulation in most fruitsis enhanced by ethylene and jasmonate. However, little is known about the hormone functions related to red pear fruit coloration or their combined effects and potential underlying mechanisms. Various treatments were used to investigate the flavonoid metabolite profile and pear transcriptome to verify the effects of ethylene and jasmonate on flavonoid biosynthesis in red pear fruits as well as the mechanism behind this. Ethylene inhibits anthocyanin biosynthesis in red Chinese pear fruits, whereas jasmonate increases anthocyanin and flavone/isoflavone biosyntheses. The branching of the jasmonate-induced flavonoid biosynthesis pathway is determined by ethylene. Co-expression network and Mfuzz analyses revealed 4,368 candidate transcripts. Additionally, ethylene suppresses PpMYB10 and PpMYB114 expression via TF repressors, ultimately decreasing anthocyanin biosynthesis. Jasmonate induces anthocyanin accumulation through transcriptional or post-translational regulation of TFs like MYB and bHLH in the absence of ethylene. However, jasmonate induces ethylene biosynthesis and the associated signaling pathway in pear, thereby decreasing anthocyanin production, increasing the availability of the precursors for flavone/isoflavone biosynthesis, and enhancing deep yellow fruit coloration. We herein present new phenotypes and fruit coloration regulatory patterns controlled by jasmonate and ethylene, and confirm that the regulation of fruit coloration is complex.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2019
Keywords
Anthocyanin, ethylene, flavone, isoflavone, jasmonate, pear, transcriptome
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:oru:diva-77711 (URN)10.1111/pbi.13287 (DOI)000497161100001 ()31675761 (PubMedID)
Note

Funding Agencies:

Earmarked Fund for China Agriculture Research System CARS-28

National Natural Science Foundation of China 31772272

China Postdoctoral Science Foundation 2019M652095

Available from: 2019-11-07 Created: 2019-11-07 Last updated: 2019-12-03Bibliographically approved
Jansen, M. A. K., Bilger, W., Hideg, É., Strid, Å. & Urban, O. (2019). Interactive effects of UV-B radiation in a complex environment. Plant physiology and biochemistry (Paris), 134, 1-8
Open this publication in new window or tab >>Interactive effects of UV-B radiation in a complex environment
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2019 (English)In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 134, p. 1-8Article in journal, Editorial material (Refereed) Published
Place, publisher, year, edition, pages
Elsevier Masson, 2019
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-69786 (URN)10.1016/j.plaphy.2018.10.021 (DOI)000455692000001 ()30385007 (PubMedID)2-s2.0-85055288326 (Scopus ID)
Funder
Knowledge Foundation, 20130164Swedish Research Council Formas, 942-2015-516
Available from: 2018-10-23 Created: 2018-10-23 Last updated: 2019-01-29Bibliographically approved
Czégény, G., Körösi, L., Strid, Å. & Hideg, É. (2019). Multiple roles for Vitamin B6in plant acclimation to UV-B. Scientific Reports, 9(1), Article ID 1259.
Open this publication in new window or tab >>Multiple roles for Vitamin B6in plant acclimation to UV-B
2019 (Swedish)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, no 1, article id 1259Article in journal (Refereed) Published
Abstract [en]

Direct and indirect roles of vitamin B6in leaf acclimation to supplementary UV-B radiation are shown in vitamin B6deficient Arabidopsis thalianamutant rsr4-1 and C24 wild type. Responses to 4 days of 3.9 kJ m-2d-1 biologically effective UV-B dose were compared in terms of leaf photochemistry, vitamer content, and antioxidant enzyme activities; complemented with a comprehensive study of vitamer ROS scavenging capacities. Under UV-B, rsr4-1 leaves lost more (34%) photochemical yield than C24 plants (24%). In the absence of UV-B, rsr4-1 leaves contained markedly less pyridoxal-5’-phosphate (PLP) than C24 ones, but levels increased up to the C24 contents in response to UV-B. Activities of class-III ascorbate and glutathione peroxidases increased in C24 leaves upon the UV-B treatment but not in the rsr4-1 mutant. SOD activities remained the same in C24 but decreased by more than 50% in rsr4-1 under UV-B. Although PLP was shown to be an excellent antioxidant in vitro, our results suggest that the UV-B protective role of B6 vitamers is realized indirectly, via supporting peroxidase defence rather than by direct ROS scavenging. We hypothesize that the two defence pathways are linked through the PLP-dependent biosynthesis of cystein and heme, affecting peroxidases.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019
National Category
Biochemistry and Molecular Biology Botany
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-70847 (URN)10.1038/s41598-018-38053-w (DOI)000457616300148 ()30718682 (PubMedID)2-s2.0-85061031153 (Scopus ID)
Funder
Swedish Research Council Formas, 942-2015-516Knowledge Foundation, 20130164
Note

Funding Agencies:

New National Excellence Program of the Ministry of Human Capacities  UNKP-17-3-III-PTE-229

Örebro University's Faculty for Business, Science and Technology  

Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences  

European Social Fund  EFOP-3.6.1.-16-2016-00004 

European Union 

Available from: 2018-12-20 Created: 2018-12-20 Last updated: 2019-02-19Bibliographically approved
Wu, M., Farkas, D., Eriksson, L. A. & Strid, Å. (2019). Proline 411 biases the conformation of the intrinsically disordered plant UVR8 photoreceptor C27 domain altering the functional properties of the peptide. Scientific Reports, 9, Article ID 818.
Open this publication in new window or tab >>Proline 411 biases the conformation of the intrinsically disordered plant UVR8 photoreceptor C27 domain altering the functional properties of the peptide
2019 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, article id 818Article in journal (Refereed) Published
Abstract [en]

UVR8 (UV RESISTANCE LOCUS 8) is a UV-B photoreceptor responsible for initiating UV-B signalling in plants. UVR8 is a homodimer in its signalling inactive form. Upon absorption of UV radiation, the protein monomerizes into its photoactivated state. In the monomeric form, UVR8 binds the E3 ubiquitin ligase COP1 (CONSTITUTIVELY PHOTOMORPHOGENIC 1), triggering subsequent UV-B-dependent photomorphogenic development in plants. Recent in vivoexperiments have shown that the UVR8 C-terminal region (aa 397-423; UVR8C27) alone is sufficient to regulate the activity of COP1. In this work, CD spectroscopy and NMR experiments showed that the UVR8C27domain was non-structured but gained secondary structure at higher temperatures leading to increased order. Bias-exchange metadynamics simulations were also performed to evaluate the free energy landscape of UVR8C27. An inverted free energy landscape was revealed, with a disordered structure in the global energy minimum. Flanking the global energy minimum, more structured states were found at higher energies. Furthermore, stabilization of the low energy disordered state was attributed to a proline residue, P411, as evident from P411A mutant data. P411 is also a key residue in UVR8 binding to COP1. UVR8C27is therefore structurally competent to function as a molecular switch for interaction of UVR8 with different binding partners since at higher free energies different structural conformations are being induced in this peptide. P411 has a key role for this function.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019
National Category
Botany Biochemistry and Molecular Biology Physical Chemistry Theoretical Chemistry
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-70355 (URN)10.1038/s41598-018-37005-8 (DOI)000456826200047 ()30692548 (PubMedID)2-s2.0-85060606046 (Scopus ID)
Funder
Swedish Research CouncilCarl Tryggers foundation
Note

Funding Agencies:

Faculty of Science at University of Gothenburg

Swedish NMR centre at the University of Gothenburg 

Sven and Lily Lawski foundation for Scientific Research

Available from: 2018-11-28 Created: 2018-11-28 Last updated: 2019-02-13Bibliographically approved
O'Hara, A., Headland, L. R., Díaz-Ramos, L. A., Morales, L. O., Strid, Å. & Jenkins, G. I. (2019). Regulation of Arabidopsis gene expression by low fluence rate UV-B independently of UVR8 and stress signaling. Photochemical and Photobiological Sciences, 18(7), 1675-1684
Open this publication in new window or tab >>Regulation of Arabidopsis gene expression by low fluence rate UV-B independently of UVR8 and stress signaling
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2019 (English)In: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 18, no 7, p. 1675-1684Article in journal (Refereed) Published
Abstract [en]

UV-B exposure of plants regulates expression of numerous genes concerned with various responses. Sudden exposure of non-acclimated plants to high fluence rate, short wavelength UV-B induces expression via stress-related signaling pathways that are not specific to the UV-B stimulus, whereas low fluence rates of UV-B can regulate expression via the UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8). However, there is little information about whether non-stressful, low fluence rate UV-B treatments can activate gene expression independently of UVR8. Here, transcriptomic analysis of wild-type and uvr8 mutant Arabidopsis exposed to low fluence rate UV-B showed that numerous genes were regulated independently of UVR8. Moreover, nearly all of these genes were distinct to those induced by stress treatments. A small number of genes were expressed at all UV-B fluence rates employed and may be concerned with activation of eustress responses that facilitate acclimation to changing conditions. Expression of the gene encoding the transcription factor ARABIDOPSIS NAC DOMAIN CONTAINING PROTEIN 13 (ANAC13) was studied to characterise a low fluence rate, UVR8-independent response. ANAC13 is induced by as little as 0.1 μmol m−2 s−1 UV-B and its regulation is independent of components of the canonical UVR8 signaling pathway COP1 and HY5/HYH. Furthermore, UV-B induced expression of ANAC13 is independent of the photoreceptors CRY1, CRY2, PHOT1 and PHOT2 and phytochromes A, B, D and E. ANAC13 expression is induced over a range of UV-B wavelengths at low doses, with maximum response at 310 nm. This study provides a basis for further investigation of UVR8 and stress independent, low fluence rate UV-B signaling pathway(s).

Place, publisher, year, edition, pages
RSC Publishing, 2019
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:oru:diva-74760 (URN)10.1039/C9PP00151D (DOI)000477947100005 ()31218318 (PubMedID)2-s2.0-85068743324 (Scopus ID)
Funder
Knowledge Foundation, 20130164Swedish Research Council Formas, 942-2015-516
Note

Funding Agencies:

UK Biotechnology and Biological Sciences research council PhD studentships at the University of Glasgow

Faculty for Business, Science, and Technology at Örebro University  

Strategic Young Researchers Recruitment Programme at Örebro University  

University of Glasgow 

Available from: 2019-06-20 Created: 2019-06-20 Last updated: 2019-11-15Bibliographically approved
Eriksson, L. A., Sirsjö, A. & Strid, Å. (2019). Tetrazole derivatives as cytochrome p450 inhibitors. us US20190040020A1.
Open this publication in new window or tab >>Tetrazole derivatives as cytochrome p450 inhibitors
2019 (English)Patent (Other (popular science, discussion, etc.))
Abstract [en]

According to the invention there is provided a compound of formula I, wherein Rand Rhave meanings given in the description, which compounds are useful in the treatment of skin disorders and other diseases.

National Category
Basic Medicine Pharmaceutical Sciences Medicinal Chemistry Cell and Molecular Biology Chemical Sciences Theoretical Chemistry Physical Chemistry Biochemistry and Molecular Biology Biophysics
Identifiers
urn:nbn:se:oru:diva-78131 (URN)
Patent
US US20190040020A1
Available from: 2019-11-26 Created: 2019-11-26 Last updated: 2019-11-26Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-3315-8835

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