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Allbrand, Marianne
Publications (8 of 8) Show all publications
Lodefalk, M., Allbrand, M. & Montgomery, S. (2022). Duration of the pushing phase of labor is inversely associated with expression of TNF, IL6, IGF1 and IGF2 in human placenta. The Journal of Maternal-Fetal & Neonatal Medicine, 35(25), 6476-6482
Open this publication in new window or tab >>Duration of the pushing phase of labor is inversely associated with expression of TNF, IL6, IGF1 and IGF2 in human placenta
2022 (English)In: The Journal of Maternal-Fetal & Neonatal Medicine, ISSN 1476-7058, E-ISSN 1476-4954, Vol. 35, no 25, p. 6476-6482Article in journal (Refereed) Published
Abstract [en]

Objective: Gene expression in placenta differs between vaginal and cesarean deliveries, but the influence of the duration of labor on placental gene expression is incompletely known. Our aim was to investigate associations between duration of labor and expression of some genes involved in growth or inflammation in human placental tissue.

Methods: Placenta samples (n = 126) were collected after an uncomplicated, singleton pregnancy and term vaginal delivery at orebro University Hospital, Sweden. Duration of labor was recorded by the midwife in the delivery room. The expression of the following genes was analyzed by RT-qPCR: tumor necrosis factor (TNF), interleukin-6 (IL6), C-X-C motif chemokine ligand 8, toll-like receptor (TLR) 2, TLR4, insulin receptor, insulin-like growth factor (IGF) 1, IGF2, leptin, hepatocyte growth factor (HGF) and HGF receptor (MET). Multivariable linear regression models were used for the evaluation of associations with labor duration adjusting for potential confounding factors. The Benjamini-Hoschberg method was used to correct for multiple testing.

Results: The expression of TNF, IL6, IGF1 and IGF2 was inversely associated with the duration of the pushing phase of labor (B coefficients (95% confidence interval) = -0.150 (-0.277 to -0.023), -0.159 (-0.289 to -0.029), -0.099 (-0.176 to -0.021), and -0.081 (-0.145 to -0.017), respectively).

Conclusions: Longer duration of pushing is associated with downregulation of the expression of genes in placenta from vaginal deliveries. Future research on gene expression in labored placenta should take into account associations with labor duration and especially the pushing phase. Potential impact of these associations on the mother, the fetus and the new-born infant should also be explored.

Place, publisher, year, edition, pages
Informa Healthcare, 2022
Keywords
Gene expression, insulin-like growth factors, interleukin-6, labor duration, placenta, pushing phase
National Category
Medical Genetics and Genomics
Identifiers
urn:nbn:se:oru:diva-91667 (URN)10.1080/14767058.2021.1916459 (DOI)000646787600001 ()33910460 (PubMedID)2-s2.0-85105914353 (Scopus ID)
Available from: 2021-05-07 Created: 2021-05-07 Last updated: 2025-02-10Bibliographically approved
Allbrand, M., Eklund, D., Cao, Y., Nilsson, K. & Lodefalk, M. (2022). Gene expression of leptin, leptin receptor isoforms and inflammatory cytokines in placentas of obese women: Associations to birth weight and fetal sex. Placenta, 117, 64-71
Open this publication in new window or tab >>Gene expression of leptin, leptin receptor isoforms and inflammatory cytokines in placentas of obese women: Associations to birth weight and fetal sex
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2022 (English)In: Placenta, ISSN 0143-4004, E-ISSN 1532-3102, Vol. 117, p. 64-71Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: Leptin signaling in placentas of obese women may influence fetal growth and may be dependent on fetal sex. The aim of this study was to investigate placental gene expression of leptin, its receptor and inflammatory cytokines in obese mothers in relation to offspring birth weight and sex.

METHODS: In total, 109 placental tissue samples from severely obese women (body mass index in first trimester ≥35 kg/m2) giving birth vaginally at term to a healthy child were included. Quantitative real-time PCR was used for the analysis of leptin (LEP), its receptor LEPR with two splice variants, interleukin (IL)1B, chemokine (C-X-C motif) ligand 8 (CXCL8), tumour necrosis factor (TNF), IL6, IL10, hypoxia-inducible factor 1-alpha (HIF1A) and insulin receptor (INSR). The subjects were divided into three groups based on LEP expression percentiles (<25th percentile; 25-75th percentile and >75th percentile).

RESULTS: A reverse U-shaped association between LEP expression and birth weight z-scores was found (R2 = 0.075, p = 0.005). Placental LEPRb expression was downregulated (p = 0.034) in those with highest LEP expression. Female infants had higher birth weight z-scores than males (0.58 (-1.49-2.88) vs 0.21 (-1.50-2.93), p = 0.020) and their placental LEPRb expression was upregulated (p = 0.047). The associations between expression of different genes differed by sex.

DISCUSSION: A reverse U-shaped relationship between placental LEP expression and offspring birth weight z-scores was found together with sexual dimorphism in LEPRb expression indicating a complex regulation of fetal growth by placental leptin signaling in maternal obesity.

Place, publisher, year, edition, pages
Elsevier, 2022
Keywords
Cytokine, Gene expression, Infant birth weight, Leptin, Obesity, Placenta
National Category
Gynaecology, Obstetrics and Reproductive Medicine
Identifiers
urn:nbn:se:oru:diva-95430 (URN)10.1016/j.placenta.2021.10.002 (DOI)000742830300010 ()34773742 (PubMedID)2-s2.0-85118757784 (Scopus ID)
Note

Funding agencies:

Research Committee of Region Örebro County

Nyckelfonden, Örebro University Hospital

Available from: 2021-11-15 Created: 2021-11-15 Last updated: 2025-02-11Bibliographically approved
Allbrand, M. (2020). Gene expression of inflammatory markers and growth factors in placenta in relation to maternal obesity and foetal and postnatal growth. (Doctoral dissertation). Örebro: Örebro University
Open this publication in new window or tab >>Gene expression of inflammatory markers and growth factors in placenta in relation to maternal obesity and foetal and postnatal growth
2020 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Maternal obesity is a growing health problem, that contributes to obstetrical complications in pregnancy, as well as neonatal morbidity and mortality. The placenta serves for gas and nutrient exchange between the mother and the foetus, and obesity may influence and modify placental growth and function. The aims of this thesis were to investigate associations between maternal obesity without associated morbidity and gene expression of inflammatory markers and growth factors in the placenta, as well as offspring birth weight and postnatal growth. 

Study I and III were designed as matched case-control studies including 32 obese women with an early pregnancy body mass index (BMI) ≥ 35.0 kg/m2, study II was an experimental study examining twelve placentas of normal weight women, and study IV was a cohort study including 109 obese women with a BMI ≥ 34.5 kg/m2. In studies I-IV analyses of gene expression were performed and in study III additionally cord blood concentrations were determined. 

No difference was found in the occurrence of placental gene expression of inflammatory markers or growth factors between obese and normal weight women, nor did the sampling site in placentas of normal weight women influence gene expression of these markers, except for leptin gene (LEP) and insulin receptor gene (INSR) expression. Ghrelin gene (GHRL) and LEP expression, as well as cord blood ghrelin and adiponectin levels, was not altered in maternal obesity, and a negatively U-shaped relationship between LEP expression and infant birth weight (BW) z-scores was observed in the placentas of obese women.

In conclusion, no statistically significant difference in gene expressions of inflammatory markers and growth factors in the placenta between severely obese and normal weight women was found. These results are in contrast with earlier studies and could be due to the fact that we examined mainly healthy obese women. The correlations we found between gene expression of leptin in the placenta and the birth weight of the infants warrants further studies.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2020. p. 93
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 221
Keywords
obesity, pregnancy, placenta, gene expression, cytokines
National Category
Other Basic Medicine
Identifiers
urn:nbn:se:oru:diva-80923 (URN)978-91-7529-354-7 (ISBN)
Public defence
2020-10-16, Örebro universitet, Campus USÖ, hörsal C1, Södra Grev Rosengatan 32, Örebro, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2020-03-31 Created: 2020-03-31 Last updated: 2020-09-21Bibliographically approved
Allbrand, M., Åman, J., Nilsson, K., Cao, Y. & Lodefalk, M. (2019). Expression of genes involved in inflammation and growth: does sampling site in human full-term placenta matter?. Journal of Perinatal Medicine, 47(5), 539-546
Open this publication in new window or tab >>Expression of genes involved in inflammation and growth: does sampling site in human full-term placenta matter?
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2019 (English)In: Journal of Perinatal Medicine, ISSN 0300-5577, E-ISSN 1619-3997, Vol. 47, no 5, p. 539-546Article in journal (Refereed) Published
Abstract [en]

Objective: To investigate the placental gene expression of substances in the inflammatory cascade and growth factors at nine different well-defined sampling sites in full-term placentas from 12 normal weight healthy non-smoking women with an uncomplicated singleton pregnancy.

Methods: All placentas (six girls and six boys) were delivered vaginally. Quantitative real-time polymerase chain reaction was used to analyze toll receptor-2 and -4, interleukin-6 and -8, tumor necrosis factor-α, leptin, ghrelin, insulin-like growth factor-1 and -2, hepatocyte growth factor, hepatocyte growth factor receptor and insulin receptor (IR).

Results: The leptin gene and the IR gene showed higher expression in lateral regions near the chorionic plate compared to central regions near the basal plate (P = 0.028 and P = 0.041, respectively).

Conclusion: Our results suggest that the sampling site may influence the gene expression for leptin and IR in placental tissue obtained from full-term normal pregnancies. We speculate that this may be due to differences in placental structure and perfusion and may be important when future studies are designed.

Place, publisher, year, edition, pages
Walter de Gruyter, 2019
Keywords
Cytokines, gene expression, growth factors, placenta, sampling
National Category
Gynaecology, Obstetrics and Reproductive Medicine
Identifiers
urn:nbn:se:oru:diva-73427 (URN)10.1515/jpm-2018-0290 (DOI)000473532900008 ()30920955 (PubMedID)2-s2.0-85063721684 (Scopus ID)
Note

Funding Agencies:

Research Committee of Region Örebro County  

Nyckelfonden, Örebro University Hospital  

Available from: 2019-04-04 Created: 2019-04-04 Last updated: 2025-02-11Bibliographically approved
Allbrand, M., Åman, J. & Lodefalk, M. (2017). Placental ghrelin and leptin expression and cord blood ghrelin, adiponectin, leptin, and C-peptide levels in severe maternal obesity. The Journal of Maternal-Fetal & Neonatal Medicine, 31(21), 2839-2846
Open this publication in new window or tab >>Placental ghrelin and leptin expression and cord blood ghrelin, adiponectin, leptin, and C-peptide levels in severe maternal obesity
2017 (English)In: The Journal of Maternal-Fetal & Neonatal Medicine, ISSN 1476-7058, E-ISSN 1476-4954, Vol. 31, no 21, p. 2839-2846Article in journal (Refereed) Published
Abstract [en]

PURPOSE: The purpose of this study is to investigate placental ghrelin and leptin expression as well as cord blood ghrelin and adiponectin levels in maternal obesity and associations between placental ghrelin expression, cord blood ghrelin levels and maternal and infant variables.

MATERIALS AND METHODS: Placental ghrelin and leptin expression were analyzed by RT-PCR in 32 severely obese and 32 matched normal-weight women. Cord blood ghrelin, adiponectin, leptin, and C-peptide concentrations were analyzed by ELISA.

RESULTS: Neither ghrelin nor leptin expression and neither cord blood ghrelin nor adiponectin levels differed between the groups. Placental ghrelin expression was associated with BMI at delivery in the obese women (r = 0.424, p = .016) and in the infants born to normal-weight women with their weight z-scores at six (r = -0.642, p = .010), nine (r = -0.441, p = .015), and 12 months of age (r = -0.402, p = .028).

CONCLUSIONS: Placental ghrelin and leptin expression as well as cord blood ghrelin and adiponectin levels do not seem to be altered in severe maternal obesity. Placenta-derived ghrelin may influence the infants' postnatal weight gain, but possibly only when the mother has normal weight.

Place, publisher, year, edition, pages
Taylor & Francis Group, 2017
Keywords
Adiponectin, birth weight, ghrelin, leptin, obesity, placenta
National Category
Gynaecology, Obstetrics and Reproductive Medicine
Identifiers
urn:nbn:se:oru:diva-61734 (URN)10.1080/14767058.2017.1358262 (DOI)000440610300007 ()28783996 (PubMedID)2-s2.0-85027047254 (Scopus ID)
Note

Funding agencies:

Research Committee of Region Örebro County

Nyckelfonden, Örebro University Hospital

Available from: 2017-11-06 Created: 2017-11-06 Last updated: 2025-02-11Bibliographically approved
Allbrand, M., Åman, J. & Lodefalk, M. (2015). Adipocytokines in placenta and cord blood in relation to maternal obesity, and foetal and postnatal growth of the child. Paper presented at 54th Annual ESPE (European Society for Paediatric Endocrinology) Meeting in Barcelona, Spain, 1-3 October 2015.. Hormone Research in Paediatrics, 82(Suppl. 1), 47-48
Open this publication in new window or tab >>Adipocytokines in placenta and cord blood in relation to maternal obesity, and foetal and postnatal growth of the child
2015 (English)In: Hormone Research in Paediatrics, ISSN 1663-2818, E-ISSN 1663-2826, Vol. 82, no Suppl. 1, p. 47-48Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Background: The nutritional and hormonal state in utero may be a link between maternal obesity and obesity in the offspring. The gene expression in placentae in pregnancies complicated by diabetes is reduced for leptin, but increased for ghrelin. It is not known whether these genes’ expressions in placentae are altered in maternal obesity.

Objectives and hypotheses: To compare obese and normal-weight women and their children concerning gene expressions of leptin and ghrelin in placentae; leptin, ghrelin, adiponectin, and C-peptide levels in cord blood, birth size and postnatal growth. Changes in the expression of these adipocytokines may lead to an altered hypothalamic sensitivity to leptin and ghrelin resulting in an increased risk of obesity in the offspring.

Method: 32 women with pre-pregnancy obesity, but otherwise healthy, were compared to 32 matched, normal-weight controls. Full-term placenta biopsies were analysed with qPCR for leptin mRNA and ghrelin mRNA. Cord blood samples were examined with ELISA for leptin, ghrelin, adiponectin, and C-peptide concentrations. Birth size and postnatal growth of the children were collected from clinical registers at the Child Health Care Units.

Results: The leptin and ghrelin gene expressions in placentae did not differ between obese and normal-weight women. The leptin concentration in cord blood was higher in children of obese mothers (P=0.021). It correlated with birth weight Z-score (r=0.467, P<0.001) and C-peptide level in cord blood (r=0.446, P<0.001). Children of obese women were slightly heavier at birth, but postnatal growth did not differ between groups. Children with birth weight  ≤−0.67 Z-score had higher ghrelin levels in cord blood than heavier children (P=0.042). The leptin level in cord blood correlated negatively with weight gain at 6 months (r=−0.332, P=0.009). The ghrelin level in cord blood correlated with weight gain at 3 months in girls (r=0.611, P=0.001), but not in boys. The adiponectin level in cord blood correlated negatively with length gain at 3 years in the obese group (r=−0.571, P=0.033), but not in the normal-weight group.

Conclusion: Leptin and ghrelin placental gene expressions are not altered in obese women, but foetal adipocytokine production may influence early postnatal growth, possibly by influencing hunger signalling or insulin levels

Place, publisher, year, edition, pages
Basel, Switzerland: S. Karger, 2015
Keywords
Obesity, ghrelin, leptin, adipocytokines, growth
National Category
Medical and Health Sciences Pediatrics
Research subject
Pediatrics
Identifiers
urn:nbn:se:oru:diva-49275 (URN)
Conference
54th Annual ESPE (European Society for Paediatric Endocrinology) Meeting in Barcelona, Spain, 1-3 October 2015.
Available from: 2016-03-11 Created: 2016-03-11 Last updated: 2022-12-20Bibliographically approved
Allbrand, M., Björkqvist, M., Nilsson, K., Östlund, I. & Åman, J. (2015). Placental gene expression of inflammatory markers and growth factors: a case control study of obese and normal weight women. Journal of Perinatal Medicine, 43(2), 159-164
Open this publication in new window or tab >>Placental gene expression of inflammatory markers and growth factors: a case control study of obese and normal weight women
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2015 (English)In: Journal of Perinatal Medicine, ISSN 0300-5577, E-ISSN 1619-3997, Vol. 43, no 2, p. 159-164Article in journal (Refereed) Published
Abstract [en]

Objective: To survey the placental gene expression of inflammatory markers and growth factors in non-smoking obese women with an uncomplicated pregnancy without associated morbidity and delivery at term compared with normal weight women.

Methods: Placental tissue samples from 32 obese women (body mass index, BMI >= 35.0 kg/m(2)) were compared with samples from 94 normal weight women (BMI 18.5-25.0 kg/m(2)) matched for age (+/- 1 year), gestational age (+/- 3 days), parity and mode of delivery. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to analyse toll receptor-2 and -4, interleukin-6 and -8, tumour necrosis factor-alpha, leptin, adiponectin, insulin-like growth factor-1 and -2, hepatocyte growth factor, hepatocyte growth factor receptor and insulin receptor.

Results: There was no significant difference in gene expression in placental tissue samples from obese and normal weight women.

Conclusion: We found no difference in the occurrence of inflammatory marker and growth factor mRNA levels in placental tissue samples from a large group of obese women without associated morbidity and with healthy infants compared to a closely matched control group of healthy normal weight women. Compared with the previous studies, this anomalous finding may be explained by the absence of associated morbidity in the obese women in our study.

Place, publisher, year, edition, pages
Walter de Gruyter, 2015
Keywords
Obesity, pregnancy
National Category
Gynaecology, Obstetrics and Reproductive Medicine Pediatrics
Research subject
Obstetrics and Gynaecology; Pediatrics
Identifiers
urn:nbn:se:oru:diva-44240 (URN)10.1515/jpm-2013-0343 (DOI)000350338000005 ()25014513 (PubMedID)2-s2.0-84945571575 (Scopus ID)
Note

Funding Agency:

Foundation for Medical Research, Orebro University Hospital

Available from: 2015-04-14 Created: 2015-04-14 Last updated: 2025-02-11Bibliographically approved
Allbrand, M., Eklund, D., Cao, Y., Åman, J., Nilsson, K. & Lodefalk, M.Gene expression of leptin, leptin receptor isoforms, and inflammatory cytokines in placentas of obese women: associations to birth weight and foetal sex.
Open this publication in new window or tab >>Gene expression of leptin, leptin receptor isoforms, and inflammatory cytokines in placentas of obese women: associations to birth weight and foetal sex
Show others...
(English)Manuscript (preprint) (Other academic)
National Category
Other Basic Medicine
Identifiers
urn:nbn:se:oru:diva-85819 (URN)
Available from: 2020-09-21 Created: 2020-09-21 Last updated: 2020-12-01Bibliographically approved
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