oru.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Strid, Hilja
Alternative names
Publications (10 of 19) Show all publications
Götlind, Y. Y., Fritsch Fredin, M., Kumawat, A. K., Strid, H., Willén, R., Rangel, I., . . . Hultgren Hörnquist, E. (2013). Interplay between Th1 and Th17 effector T cell pathways in the pathogenesis of spontaneous colitis and colon cancer in the Gai2-deficient mouse. International Immunology, 25(1), 35-44
Open this publication in new window or tab >>Interplay between Th1 and Th17 effector T cell pathways in the pathogenesis of spontaneous colitis and colon cancer in the Gai2-deficient mouse
Show others...
2013 (English)In: International Immunology, ISSN 0953-8178, E-ISSN 1460-2377, Vol. 25, no 1, p. 35-44Article in journal (Refereed) Published
Abstract [en]

Gαi2-deficient mice spontaneously develop colitis. Using xMAP technology and RT-PCR, we investigated cytokine/chemokine profiles during histologically defined phases of disease: (i) no/mild, (ii) moderate, (iii) severe colitis without dysplasia/cancer and (iv) severe colitis with dysplasia/cancer, compared with age-matched wild-type (WT) littermates. Colonic dysplasia was observed in 4/11 mice and cancer in 1/11 mice with severe colitis. The histology correlated with progressive increases in colon weight/cm and spleen weight, and decreased thymus weight, all more advanced in mice with dysplasia/cancer. IL-1β, IL-6, IL-12p40, IL-17, TNF-α, CCL2 and CXCL1 protein levels in colons, but not small intestines increased with colitis progression and were significantly increased in mice with moderate and severe colitis compared with WT mice, irrespective of the absence/presence of dysplasia/cancer. CCL5 did not change during colitis progression. Colonic IL-17 transcription increased 40- to 70-fold in all stages of colitis, whereas IFN-γ mRNA was gradually up-regulated 12- to 55-fold with colitis progression, and further to 62-fold in mice with dysplasia/cancer. IL-27 mRNA increased 4- to 15-fold during the course of colitis, and colonic IL-21 transcription increased 3-fold in mice with severe colitis, both irrespective of the absence/presence of dysplasia/cancer. FoxP3 transcription was significantly enhanced (3.5-fold) in mice with moderate and severe colitis, but not in mice with dysplasia/cancer, compared with WT mice. Constrained correspondence analysis demonstrated an association between increased protein levels of TNF-α, CCL2, IL-1β, IL-6 and CXCL1 and dysplasia/cancer. In conclusion, colonic responses are dominated by a mixed T(h)1/T(h)17 phenotype, with increasing T(h)1 cytokine transcription with progression of colitis in Gαi2(-/-) mice.

Place, publisher, year, edition, pages
Oxford, United Kingdom: Oxford University Press, 2013
Keywords
Chemokines, constrained correspondence analysis, dysplasia, inflammatory bowel disease, real-time RT–PCR
National Category
Medical and Health Sciences Gastroenterology and Hepatology
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-25590 (URN)10.1093/intimm/dxs089 (DOI)000313127400004 ()22962436 (PubMedID)2-s2.0-84872130510 (Scopus ID)
Funder
Swedish Research Council, 2008-4075Swedish Cancer Society, CAN 2008/591
Note

Funding Agency:

Sahlgrenska Academy 

Sahlgrenska University Hospital Foundation for Clinical research 

Swedish Society of Medicine 

Available from: 2012-08-30 Created: 2012-08-30 Last updated: 2019-03-26Bibliographically approved
Kumawat, A. K., Strid, H., Tysk, C., Bohr, J. & Hultgren-Hörnquist, E. (2013). Microscopic colitis patients demonstrate a mixed Th17/Tc17 and Th1/Tc1 mucosal cytokine profile. Molecular Immunology, 55(3-4), 355-364
Open this publication in new window or tab >>Microscopic colitis patients demonstrate a mixed Th17/Tc17 and Th1/Tc1 mucosal cytokine profile
Show others...
2013 (English)In: Molecular Immunology, ISSN 0161-5890, E-ISSN 1872-9142, Vol. 55, no 3-4, p. 355-364Article in journal (Refereed) Published
Abstract [en]

Background:

Microscopic colitis (MC) is a chronic inflammatory bowel disorder of unknown aetiology comprising collagenous colitis (CC) and lymphocytic colitis (LC). Data on the local cytokine profile in MC is limited. This study investigated the T helper (Th) cell and cytotoxic T lymphocyte (CTL) mucosal cytokine profile at messenger and protein levels in MC patients.

Methods:

Mucosal biopsies from CC (n = 10), LC (n = 5), and CC or LC patients in histopathological remission (CC-HR, n = 4), (LC-HR, n = 6), ulcerative colitis (UC, n = 3) and controls (n = 10) were analysed by real-time PCR and Luminex for expression/production of IL-1 beta, -4, -5, -6, -10, -12, -17, -21, -22, -23, IFN-gamma, TNF-alpha, T-bet and RORC2.

Results:

Mucosal mRNA but not protein levels of IFN-gamma and IL-12 were significantly up regulated in CC, LC as well as UC patients compared to controls. Transcription of the Th1 transcription factor T-bet was significantly enhanced in CC but not LC patients. mRNA levels for IL-17A, IL-21, IL-22 and IL-6 were significantly up regulated in CC and LC patients compared to controls, albeit less than in UC patients. Significantly enhanced IL-21 protein levels were noted in both CC and LC patients. IL-6 protein and IL-1 beta mRNA levels were increased in CC and UC but not LC patients. Increased mucosal mRNA levels of IFN-gamma, IL-21 and IL-22 were correlated with higher clinical activity, recorded as the number of bowel movements per day, in MC patients.

Although at lower magnitude, IL-23A mRNA was upregulated in CC and LC, whereas TNF-alpha protein was increased in CC, LC as well as in UC patients.

Neither mRNA nor protein levels of IL-4, IL-5 or IL-10 were significantly changed in any of the colitis groups. LC-HR and especially CC-HR patients had normalized mRNA and protein levels of the above cytokines compared to LC and CC patients. No significant differences were found between LC and CC in cytokine expression/production.

Conclusion:

LC and CC patients demonstrate a mixed Th17/Tc17 and Th1/Tc1 mucosal cytokine profile.

Keywords
T cells, Mucosal cytokines, Microscopic colitis, Collagenous colitis, Lymphocytic colitis
National Category
Immunology in the medical area
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-29849 (URN)10.1016/j.molimm.2013.03.007 (DOI)000319540200020 ()
Available from: 2013-06-28 Created: 2013-06-28 Last updated: 2019-03-26Bibliographically approved
Kumawat, A. K., Strid, H., Elgbratt, K., Tysk, C., Bohr, J. & Hultgren Hörnquist, E. (2013). Microscopic colitis patients have increased frequencies of Ki67+proliferating and CD45RO+ active/memory CD8+ and CD4+8+ mucosal T cells. Journal of Crohn's & Colitis, 7(9), 694-705
Open this publication in new window or tab >>Microscopic colitis patients have increased frequencies of Ki67+proliferating and CD45RO+ active/memory CD8+ and CD4+8mucosal T cells
Show others...
2013 (English)In: Journal of Crohn's & Colitis, ISSN 1873-9946, E-ISSN 1876-4479, Vol. 7, no 9, p. 694-705Article in journal (Refereed) Published
Abstract [en]

Background: Collagenous colitis (CC) and lymphocytic colitis (LC) are chronic inflammatory bowel disorders of unknown etiology. This study investigated phenotypic characteristics of the mucosal lymphocytes in CC and LC.

Methods: Lamina propria and intraepithelial lymphocytes (LPLs, IELs) isolated from mucosal biopsies from CC (n = 7), LC (n = 6), as well as LC or CC patients in histopathological remission, (LC-HR) (n = 6) and CC-HR (n = 4) and non-inflamed controls (n = 10) were phenotypically characterized by four-color flow cytometry.

Results: The proportions of CD8+ IELs were increased in CC and LC (p < 0.01) compared to controls. Increased proportions of CD45RO+CD8+ IELs and LPLs were observed in LC and even more in CC patients (p < 0.01). Both CC (p < 0.05) and LC patients had elevated proportions of CD4+8+ IELs and LPLs compared to controls. The proportions of CD45RO+ cells were increased in CD4+8+ IELs and LPLs (p < 0.05) in CC and LC patients compared to controls. Both CC (p < 0.05) and LC patients had higher proportions of Ki67+CD8+ IELs and LPLs compared to controls.

In contrast, decreased proportions of CD4+ LPLs were observed in CC and LC as well as CD4+ IELs in LC compared to controls. Increased proportions of Ki67+CD4+ IELs and LPLs (p < 0.05) were observed in CC and LC patients. CC-HR but not LC-HR patients demonstrated normalized proportions of both IELs and LPLs compared to CC and LC patients respectively.

Conclusion: LC and CC patients have differences in mucosal lymphocyte subsets, with increased proportions of Ki67+ and CD45RO+ CD8+ and CD4+8+ mucosal T cells.

Place, publisher, year, edition, pages
Oxford, United Kingdom: Oxford University Press, 2013
Keywords
Collagenous colitis, Lymphocytic colitis, Flow cytometry, Lamina propria lymphocytes, Intraepithelial lymphocytes
National Category
Medical and Health Sciences Gastroenterology and Hepatology
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-25588 (URN)10.1016/j.crohns.2012.08.014 (DOI)000323995900002 ()22995775 (PubMedID)
Available from: 2012-08-30 Created: 2012-08-30 Last updated: 2019-03-26Bibliographically approved
Varelogianni, G., Hussain, R., Strid, H., Oliynyk, I., Roomans, G. M. & Johannesson, M. (2013). The effect of ambroxol on chloride transport, CFTR and ENaC in cysticfibrosis airway epithelial cells. Cell Biology International, 37(11), 1149-1156
Open this publication in new window or tab >>The effect of ambroxol on chloride transport, CFTR and ENaC in cysticfibrosis airway epithelial cells
Show others...
2013 (English)In: Cell Biology International, ISSN 1065-6995, E-ISSN 1095-8355, Vol. 37, no 11, p. 1149-1156Article in journal (Refereed) Published
Abstract [en]

Ambroxol, a mucokinetic anti-inflammatory drug, has been used for treatment of cystic fibrosis (CF). The respiratoryepitheliumis covered by the airway surface liquid (ASL), the thickness and composition of which is determined by Cl efflux viathe cystic fibrosis transmembrane conductance regulator (CFTR) and Naþ influx via the epithelial Naþ channel (ENaC). In cellsexpressing wt-CFTR, ambroxol increased the Cl- conductance, but not the bicarbonate conductance of the CFTR channels.Weinvestigated whether treatment with ambroxol enhances chloride transport and/or CFTR and ENaC expression in CF airwayepithelial cells (CFBE) cells. CFBE cells were treated with 100 mM ambroxol for 2, 4 or 8 h. mRNA expression for CFTR andENaC subunits was analysed by real-time polymerase chain reaction (RT-PCR); protein expression was measured by Westernblot. The effect of ambroxol on Cl− transport was measured by Cl− efflux measurements with a fluorescent chloride probe.Ambroxol significantly stimulated Cl− efflux from CFBE cells (a sixfold increase after 8 h treatment), and enhanced theexpression of the mRNA of CFTR and a-ENaC, and of the CFTR protein. No significant difference was observed in b-ENaCafter exposure to ambroxol, whereasmRNA expression of g-ENaC was reduced. No significant effects of ambroxol on the ENaCsubunits were observed by Western blot. Ambroxol did not significantly affect the intracellular Ca2+ concentration.Upregulation of CFTR and enhanced Cl efflux after ambroxol treatment should promote transepithelial ion and watertransport, which may improve hydration of the mucus, and therefore be beneficial to CF-patients.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell, 2013
Keywords
Airway epithelium, ambroxol, cystic fibrosis, Cl− efflux, CFTR; ENaC
National Category
Medical and Health Sciences Cell Biology
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-32769 (URN)10.1002/cbin.10146 (DOI)000325489500002 ()23765701 (PubMedID)2-s2.0-84885861654 (Scopus ID)
External cooperation:
Funder
Swedish Heart Lung FoundationSwedish Research Council
Note

Funding Agencies:

Swedish Science Research Council 

Available from: 2013-12-13 Created: 2013-12-13 Last updated: 2017-12-06Bibliographically approved
Strid, H., Kumawat, A., Tysk, C., Hultgren Hörnquist, E. & Bohr, J. (2012). Genuttrycket för Renin och IL-6 i kolonmucosan är förändrad vid kollagen kolit. In: : . Paper presented at Svenska Gastrodagarna, Malmö, 8-11 Maj, 2012.
Open this publication in new window or tab >>Genuttrycket för Renin och IL-6 i kolonmucosan är förändrad vid kollagen kolit
Show others...
2012 (Swedish)Conference paper, Oral presentation only (Other academic)
National Category
Immunology in the medical area
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-24430 (URN)
Conference
Svenska Gastrodagarna, Malmö, 8-11 Maj, 2012
Available from: 2012-08-15 Created: 2012-08-15 Last updated: 2019-03-26Bibliographically approved
Kumawat, A. K., Strid, H., Tysk, C., Bohr, J. & Hultgren-Hörnquist, E. (2012). Patienter med mikroskopisk kolit har blandad Th1/Th17 samt CTL-associerad cytokinprofil. In: : . Paper presented at Svenska Gastrodagarna, Malmö, 8-11 Maj, 2012.
Open this publication in new window or tab >>Patienter med mikroskopisk kolit har blandad Th1/Th17 samt CTL-associerad cytokinprofil
Show others...
2012 (Swedish)Conference paper, Oral presentation only (Other academic)
National Category
Immunology in the medical area
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-24433 (URN)
Conference
Svenska Gastrodagarna, Malmö, 8-11 Maj, 2012
Available from: 2012-08-15 Created: 2012-08-15 Last updated: 2019-03-26Bibliographically approved
Strid, H., Kumawat, A. K., Tysk, C., Hultgren Hörnquist, E. & Bohr, J. (2011). Altered gene expression of IL-6 and rennin in colonic biopsies from collagenous colitis and ulcerative colitis compared to healthy controls. Paper presented at 19th United European Gastroenterology Week (UEGW) October 22-26, 2011. Gut, 60(Suppl. 3), Article ID A317.
Open this publication in new window or tab >>Altered gene expression of IL-6 and rennin in colonic biopsies from collagenous colitis and ulcerative colitis compared to healthy controls
Show others...
2011 (English)In: Gut, ISSN 0017-5749, E-ISSN 1468-3288, Vol. 60, no Suppl. 3, article id A317Article in journal, Meeting abstract (Refereed) Published
Place, publisher, year, edition, pages
BMJ Publishing Group Ltd, 2011
National Category
Immunology in the medical area
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-24437 (URN)
Conference
19th United European Gastroenterology Week (UEGW) October 22-26, 2011
Available from: 2012-08-15 Created: 2012-08-15 Last updated: 2019-03-26Bibliographically approved
Kumawat, A. K., Strid, H., Elgbratt, K., Nyhlin, N., Tysk, C., Bohr, J. & Hultgren Hörnquist, E. (2011). Collagenous colitis patients demonstrate a Th1/CTL-associated gene expression profile with increased frequencies of Ki67+ proliferating and CD45RO+ activated/ memory CD8+ and CD4+8+ mucosal T cells. In: : . Paper presented at International Congress of Mucosal Immunology, Paris, July 5-9, 2011.
Open this publication in new window or tab >>Collagenous colitis patients demonstrate a Th1/CTL-associated gene expression profile with increased frequencies of Ki67+ proliferating and CD45RO+ activated/ memory CD8+ and CD4+8+ mucosal T cells
Show others...
2011 (English)Conference paper, Oral presentation only (Other academic)
National Category
Immunology in the medical area
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-24442 (URN)
Conference
International Congress of Mucosal Immunology, Paris, July 5-9, 2011
Available from: 2012-08-15 Created: 2012-08-15 Last updated: 2019-03-26Bibliographically approved
Kumawat, A. K., Strid, H., Elgbratt, K., Nyhlin, N., Tysk, C., Bohr, J. & Hultgren Hörnquist, E. (2011). Collagenous colitis patients demonstrate a Th1/CTL-associated gene expression profile with increased frequencies of Ki67+ proliferating and CD45RO+ activated/memory CD8+ and CD4+8+ mucosal T cells. Paper presented at 19th United European Gastroenterology Week (UEGW), Stockholm, Sweden, October 22-26, 2011. Gut, 60(Suppl. 3), A318
Open this publication in new window or tab >>Collagenous colitis patients demonstrate a Th1/CTL-associated gene expression profile with increased frequencies of Ki67+ proliferating and CD45RO+ activated/memory CD8+ and CD4+8+ mucosal T cells
Show others...
2011 (English)In: Gut, ISSN 0017-5749, E-ISSN 1468-3288, Vol. 60, no Suppl. 3, p. A318-Article in journal, Meeting abstract (Refereed) Published
Place, publisher, year, edition, pages
BMJ Publishing Group Ltd, 2011
National Category
Immunology in the medical area
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-24434 (URN)
Conference
19th United European Gastroenterology Week (UEGW), Stockholm, Sweden, October 22-26, 2011
Available from: 2012-08-15 Created: 2012-08-15 Last updated: 2019-03-26Bibliographically approved
Kumawat, A., Götlind, Y.-Y., Fritsch Fredin, M., Willén, R., Chazot, P., Strid, H. & Hultgren Hörnquist, E. (2011). Modulation of histamine 4 receptor mRNA and protein expression in Gai2-deficient mice during colitis progression. Paper presented at 40th meeting of Scandinavian Society for Immunology and the winter school of immunology, Geilo, Norway, April 5-8, 2011. Scandinavian Journal of Immunology, 73(4), 373-373
Open this publication in new window or tab >>Modulation of histamine 4 receptor mRNA and protein expression in Gai2-deficient mice during colitis progression
Show others...
2011 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 73, no 4, p. 373-373Article in journal, Meeting abstract (Refereed) Published
Place, publisher, year, edition, pages
Wiley-Blackwell, 2011
National Category
Immunology in the medical area
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-24452 (URN)10.1111/j.1365-3083.2011.02516.x (DOI)000287871800076 ()
Conference
40th meeting of Scandinavian Society for Immunology and the winter school of immunology, Geilo, Norway, April 5-8, 2011
Available from: 2012-08-15 Created: 2012-08-15 Last updated: 2019-03-26Bibliographically approved
Organisations

Search in DiVA

Show all publications