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Johansson, Carina B.
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Publications (10 of 91) Show all publications
Nyberg, J., Hertzman, S., Svensson, B. & Johansson, C. B. (2013). Osseointegration of implants in irradiated bone with and without hyperbaric oxygen treatment: an experimental study in rat tibiae. International Journal of Oral & Maxillofacial Implants, 28(3), 739-746
Open this publication in new window or tab >>Osseointegration of implants in irradiated bone with and without hyperbaric oxygen treatment: an experimental study in rat tibiae
2013 (English)In: International Journal of Oral & Maxillofacial Implants, ISSN 0882-2786, E-ISSN 1942-4434, Vol. 28, no 3, p. 739-746Article in journal (Refereed) Published
Abstract [en]

Purpose: Hyperbaric oxygen (HBO) has been recommended to enhance implant osseointegration in irradiated bone. The aim of this study was to further investigate the effects of HBO on implant integration in irradiated bone tissue.

Materials and Methods: The present study was an experimental intraindividual study in 16 rats. A single fraction of 20 Gy external irradiation was applied to one rat hind leg, while the other served as a nonirradiated control. Three days after radiation, two implants were inserted in each tibial tuberosity. The rats were divided into two groups: non-HBO treated (group 1) and HBO treated (group 2). Five weeks after radiation, removal torque tests were performed. Implants with surrounding tissue were processed to undecalcified cut and ground sections for histomorphometric evaluations of bone-to-implant contact and bone area. Retrieved bones were also investigated with dual-energy x-ray absorptiometry.

Results: The non-HBO treated rats (group 1) demonstrated higher, but not statistically significantly higher, values in the nonirradiated leg for all investigated parameters compared to the HBO-treated rats (group 2). However, the mean value for bone area was significantly higher in the irradiated sides compared to the nonirradiated control sides.

Conclusions: In the present study, HBO treatment did not have a significant impact on osseointegration of implants in irradiated bone.

Place, publisher, year, edition, pages
Quintessence Publishing Co., Inc., 2013
Keywords
bone, dental implant, hyperbaric oxygen therapy, osseointegration, radiation therapy
National Category
Dentistry
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-30313 (URN)10.11607/jomi.3083 (DOI)000322078500018 ()23748304 (PubMedID)2-s2.0-84898313432 (Scopus ID)
Note

Funding agency: Särfonderna, Örebro University Hospital, Örebro, Sweden;  Hjalmar Svenssons Research Fund, Goteborg, Sweden 

Available from: 2013-08-23 Created: 2013-08-23 Last updated: 2018-04-16Bibliographically approved
Johansson, C. B., Jimbo, R. & Stefenson, P. (2012). Ex vivo and in vivo biomechanical test of implant attachment to various materials: introduction of a new user-friendly removal torque equipment. Clinical Implant Dentistry and Related Research, 14(4), 603-611
Open this publication in new window or tab >>Ex vivo and in vivo biomechanical test of implant attachment to various materials: introduction of a new user-friendly removal torque equipment
2012 (English)In: Clinical Implant Dentistry and Related Research, ISSN 1523-0899, E-ISSN 1708-8208, Vol. 14, no 4, p. 603-611Article in journal (Refereed) Published
Abstract [en]

Objective:The removal torque (RTQ) analysis is commonly used for biomechanical evaluation of osseointegration. The overall aim of this study was to verify results obtained with a newly developed equipment for biomechanical testing of osseointegration.

Methods: Verification of the new equipment for biomechanical tests involved three experiments: Part I, comparison of RTQ between implants placed in four different types of dental synthetic plasters. Part II, comparison of RTQ between custom made, experimentally used implants to self-tapping, commercially available implants molded in the same type of dental plaster. Part III, comparison of RTQ between commercially pure titanium implants to Ti6Al4V implants placed in rabbit bone, 6 weeks after insertion. Briefly, for all experiments, the peak RTQ values and the removal process were recorded every 0.01 seconds up to 10 seconds. After the measurements, peak RTQ values were converted to shear strength.

Results: The developed equipment sensitively responded to the changes of properties related to the molding plasters, implant topographies, and materials. The monitored graphs corresponded well to the expected properties of the different implants and tested materials.

Conclusion: The new RTQ equipment proved to be accurate and could add new knowledge in understanding the biomechanical aspects of osseointegration.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2012
Keywords
Ex vivo, in vivo, removal torque, shear strength
National Category
Dentistry
Identifiers
urn:nbn:se:oru:diva-11602 (URN)10.1111/j.1708-8208.2010.00296.x (DOI)000307011400016 ()20662862 (PubMedID)2-s2.0-84864498020 (Scopus ID)
Funder
Swedish Research Council, 621-2005-3402
Note

Funding Agency:

Örebro University U2000

Available from: 2010-08-18 Created: 2010-08-18 Last updated: 2017-12-12Bibliographically approved
Jimbo, R., Sotres, J., Johansson, C., Breding, K., Currie, F. & Wennerberg, A. (2012). The biological response to three different nanostructures applied on smooth implant surfaces. Clinical Oral Implants Research, 23(6), 706-712
Open this publication in new window or tab >>The biological response to three different nanostructures applied on smooth implant surfaces
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2012 (English)In: Clinical Oral Implants Research, ISSN 0905-7161, E-ISSN 1600-0501, Vol. 23, no 6, p. 706-712Article in journal (Refereed) Published
Abstract [en]

Objective: To evaluate the biological effects of three calcium phosphate (CaP) coatings with nanostructures on relatively smooth implant surfaces.

Material and methods: Stable CaP nanoparticle suspensions of different particle sizes and structures were coated onto implants by immersion and subsequent heat treatment. An uncoated implant was used as the control. After topographical and chemical characterizations, implants were randomly inserted into rabbit tibiae for removal torque (RTQ) testing. To confirm the biological reaction, implants were placed in the bilateral femurs of three rabbits.

Results: The topographical characterization showed that each surface had different nanostructural characteristics and X-ray photon spectroscopy showed various CaP compositions. The control and test groups had different nanotopographies; however, the differences among the test groups were only significant for Surfaces B and C and the rest were insignificant. The RTQ tests showed significantly higher values in two test groups (Surface A and Surface C). Histologically, no adverse effects were seen in any group. Histomorphometrical evaluation showed comparable or better osseointegration along the implant threads in the test groups.

Conclusion: The three different CaP coatings with nanostructures on the implant surfaces had enhancing effects on osseointegration. Along with the surface nanotopography, the CaP chemistry might have influenced the biological outcomes.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell, 2012
Keywords
Animal experiments, biomaterials, bone implant interactions, surface chemistry
National Category
Medical and Health Sciences Dentistry
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-23078 (URN)10.1111/j.1600-0501.2011.02182.x (DOI)000303119000008 ()21488968 (PubMedID)2-s2.0-84860229593 (Scopus ID)
Funder
Swedish Research CouncilKnowledge Foundation
Note

Funding Agencies:

Hjalmar Svensson Research Foundation

Örebro University 

Available from: 2012-05-31 Created: 2012-05-31 Last updated: 2018-09-11Bibliographically approved
Kang, B.-S., Sul, Y.-T., Johansson, C. B., Oh, S.-J., Lee, H.-J. & Albrektsson, T. (2012). The effect of calcium ion concentration on the bone response to oxidized titanium implants. Clinical Oral Implants Research, 23(6), 690-697
Open this publication in new window or tab >>The effect of calcium ion concentration on the bone response to oxidized titanium implants
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2012 (English)In: Clinical Oral Implants Research, ISSN 0905-7161, E-ISSN 1600-0501, Vol. 23, no 6, p. 690-697Article in journal (Refereed) Published
Abstract [en]

Aim: To investigate the effect of calcium concentration on the bone tissue response to Ca-incorporated titanium implants.

Materials and methods: Two titanium surfaces containing 4.2% and 6.6% calcium were prepared using the micro-arc oxidation process. The implants were inserted in the tibia of nine New Zealand White rabbits. After 6 weeks of healing, the bone response to the implants was quantitatively compared by biomechanical and histomorphometrical measurements.

Results: Ca 4.2% and Ca 6.6% containing implants revealed no distinctive differences in their qualitative surface chemistry; chemical bonding state of Ca in titanium oxide was mainly calcium titanates. No significant differences were observed between two implants in peak removal torque and shear strength comparisons (P>0.05). Histomorphometrical analyses presented no significant differences in bonemetal contact, bone area and newly formed bone measurements between two implants (P>0.05).

Conclusions: From biomechanical and histomorphometrical measurements, the two calcium concentrations in this study did not differ significantly with respect to their influence on the bone tissue response. This similar bone response in rabbit tibiae may be explained by the similarity of the qualitative Ca chemistry in titanium surfaces.

Place, publisher, year, edition, pages
Malden, USA: Wiley-Blackwell, 2012
Keywords
Bone response, calcium concentration, micro-arc oxidation, shear strength, surface chemistry, titanium implant
National Category
Medical and Health Sciences Dentistry
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-23077 (URN)10.1111/j.1600-0501.2011.02177.x (DOI)000303119000006 ()21443613 (PubMedID)2-s2.0-84860247757 (Scopus ID)
Funder
Swedish Research Council
Note

Funding Agencies:

Ministry of Education, Science and Technology of Korea 

Hjalmar Svensson Research Foundation

Sylvans Foundation 

Available from: 2012-05-31 Created: 2012-05-31 Last updated: 2018-05-08Bibliographically approved
Reigstad, O., Johansson, C. B., Stenport, V., Wennerberg, A., Reigstad, A. & Rökkum, M. (2011). Different patterns of bone fixation with hydroxyapatite and resorbable CaP coatings in the rabbit tibia at 6, 12, and 52 weeks. Journal of Biomedical Materials Research. Part B - Applied biomaterials, 99B(1), 14-20
Open this publication in new window or tab >>Different patterns of bone fixation with hydroxyapatite and resorbable CaP coatings in the rabbit tibia at 6, 12, and 52 weeks
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2011 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 99B, no 1, p. 14-20Article in journal (Refereed) Published
Abstract [en]

Applying bioactive coatings on orthopedic implants can increase the fixation and long-term implant survival. In our study, we compared a resorbable electrochemically deposited calcium phosphate coating (Bonit (R)) to a thin (40 mu m) plasma-sprayed hydroxyapatite (HA) coating, applied on grit-blasted screw-shaped Ti-6Al-4V implants in the cortical region of rabbit tibia, implanted for 6, 12, and 52 weeks. The removal torque results demonstrated stronger bone-to-implant fixation for the HA than Bonit-coated screws at 6 and 12 weeks. After 52 weeks, the fixation was in favor of the Bonit-coated screws, but the difference was statistically insignificant. Coat flaking and delamination of the HA with multinucleated giant cell activity and bone resorption observed histologically seemed to preclude any significant increase in fixation comparing the HA implants at 6 versus 12 weeks and 12 versus 52 weeks. The Bonit-coated implants exhibited increasing fixation from 6 to 12 weeks and from 12 to 52 weeks, and the coat was resorbed within 6 weeks, with minimal activity of multinucleated giant cells or bone resorption. A different fixation pattern was observed for the two coatings with a sharper but time limited increase in fixation for the HA-coated screws, and a slower but more steadily increasing fixation pattern for the Bonit-coated screws. The side effects were more serious for the HA coating and limiting the expected increase in fixation with time. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 99B: 14-20, 2011.

National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-18638 (URN)10.1002/jbm.b.31866 (DOI)000294912400002 ()
Available from: 2011-09-30 Created: 2011-09-29 Last updated: 2017-12-08Bibliographically approved
Sarve, H., Lindblad, J., Borgefors, G. & Johansson, C. B. (2011). Extracting 3D information on bone remodeling in the proximity of titanium implants in SR mu CT image volumes. Computer Methods and Programs in Biomedicine, 102(1), 25-34
Open this publication in new window or tab >>Extracting 3D information on bone remodeling in the proximity of titanium implants in SR mu CT image volumes
2011 (English)In: Computer Methods and Programs in Biomedicine, ISSN 0169-2607, E-ISSN 1872-7565, Vol. 102, no 1, p. 25-34Article in journal (Refereed) Published
Abstract [en]

Bone-implant integration is measured in several ways. Traditionally and routinely, 2D histological sections of samples, containing bone and the biomaterial, are stained and analyzed using a light microscope. Such histological section provides detailed cellular information about the bone regeneration in the proximity of the implant. However, this information reflects the integration in only a very small fraction, a 10 mu m thick slice, of the sample. In this study, we show that feature values quantified on 2D sections are highly dependent on the orientation and the placement of the section, suggesting that a 3D analysis of the whole sample is of importance for a more complete judgment of the bone structure in the proximity of the implant. We propose features describing the 3D data by extending the features traditionally used for 20-analysis. We present a method for extracting these features from 3D image data and we measure them on five 3D SR mu CT image volumes. We also simulate cuts through the image volume positioned at all possible section positions. These simulations show that the measurement variations due to the orientation of the section around the center line of the implant are about 30%.

Place, publisher, year, edition, pages
Elsevier, 2011
Keywords
Image analysis, 3D image volume, Bone remodeling, SR mu CT, Feature estimation, Osseointegration
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-17093 (URN)10.1016/j.cmpb.2010.12.011 (DOI)000289184500003 ()21269725 (PubMedID)2-s2.0-79952361014 (Scopus ID)
Available from: 2011-09-05 Created: 2011-09-02 Last updated: 2017-12-08Bibliographically approved
Sul, Y.-T., Johansson, C. B. & Albrektsson, T. (2010). A novel in vivo method for quantifying the interfacial biochemical bond strength of bone implants. Journal of the Royal Society Interface, 7(42), 81-90
Open this publication in new window or tab >>A novel in vivo method for quantifying the interfacial biochemical bond strength of bone implants
2010 (English)In: Journal of the Royal Society Interface, ISSN 1742-5689, E-ISSN 1742-5662, Vol. 7, no 42, p. 81-90Article in journal (Refereed) Published
Abstract [en]

Quantifying the in vivo interfacial biochemical bond strength of bone implants is a biological challenge. We have developed a new and novel in vivo method to identify an interfacial biochemical bond in bone implants and to measure its bonding strength. This method, named biochemical bond measurement (BBM), involves a combination of the implant devices to measure true interfacial bond strength and surface property controls, and thus enables the contributions of mechanical interlocking and biochemical bonding to be distinguished from the measured strength values. We applied the BBM method to a rabbit model, and observed great differences in bone integration between the oxygen (control group) and magnesium (test group) plasma immersion ion-implanted titanium implants (0.046 versus 0.086 MPa, n=10, p=0.005). The biochemical bond in the test implants resulted in superior interfacial behaviour of the implants to bone: (i) close contact to approximately 2 μm thin amorphous interfacial tissue, (ii) pronounced mineralization of the interfacial tissue, (iii) rapid bone healing in contact, and (iv) strong integration to bone. The BBM method can be applied to in vivo experimental models not only to validate the presence of a biochemical bond at the bone–implant interface but also to measure the relative quantity of biochemical bond strength. The present study may provide new avenues for better understanding the role of a biochemical bond involved in the integration of bone implants.

Place, publisher, year, edition, pages
London, United Kingdom: Royal Society, 2010
Keywords
bone-implant interface, interfacial biochemical bond, bonding strength measurement, titanium, metal plasma source ion implantation, surface property
National Category
Biomaterials Science
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-11357 (URN)10.1098/rsif.2009.0060 (DOI)000271951100007 ()19369221 (PubMedID)2-s2.0-70249146653 (Scopus ID)
Available from: 2010-07-06 Created: 2010-07-06 Last updated: 2018-04-19Bibliographically approved
Romanos, G. E., Traini, T., Johansson, C. B. & Piattelli, A. (2010). Biologic Width and Morphologic Characteristics of Soft Tissues Around Immediately Loaded Implants: Studies Performed on Human Autopsy Specimens. Journal of Periodontology, 81(1), 70-78
Open this publication in new window or tab >>Biologic Width and Morphologic Characteristics of Soft Tissues Around Immediately Loaded Implants: Studies Performed on Human Autopsy Specimens
2010 (English)In: Journal of Periodontology, ISSN 0022-3492, E-ISSN 1943-3670, Vol. 81, no 1, p. 70-78Article in journal (Refereed) Published
Abstract [en]

Background: Esthetics and the health of oral implants are based upon the soft tissue reaction and biologic width (BW).

Methods: Twelve dental implants were placed in the maxilla and mandible of a patient who smoked. Permanent standard abutments and temporary restorations were immediately fixed in place during the surgery stage. The definitive restorations were placed 4 months after loading without removal of the original abutments. After 10 months, the patient died, and the implants were removed en block and processed for histology.

Results: The BW in the maxilla was 6.5 ± 2.5 mm, whereas in the mandible, it was 4.8 ± 1.3 mm (P = 0.017). The sulcular epithelium (SE) in the maxilla was 2.7 ± 0.8 mm, whereas in the mandible, it was 1.7 ± 0.4 mm (P <0.001). The junctional epithelium (JE) in the maxilla was 1.3 ± 0.4 mm, whereas in the mandible, it was 1.5 ± 0.5 mm (P = 0.164). The connective tissue (CT) in the maxilla was 2.5 ± 1.3 mm, whereas in the mandible, it was 1.6 ± 0.4 mm (P = 0.006). In the maxillary bone, the BW, SE, and CT were significantly longer than in the mandible, whereas for the JE, no statistically significant difference was observed.

Conclusion: The soft tissue organization around dental implants was different for upper and lower jawbones.

Place, publisher, year, edition, pages
Chicago, USA: American Academy of Periodontology, 2010
Keywords
Connective tissue, dental implants; dental prosthesis, implant supported, epithelial cells, junctional epithelium
National Category
Medical and Health Sciences Dentistry
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-11354 (URN)10.1902/jop.2009.090364 (DOI)000283020200011 ()20059419 (PubMedID)2-s2.0-77649207617 (Scopus ID)
Available from: 2010-07-06 Created: 2010-07-06 Last updated: 2018-04-19Bibliographically approved
Mordenfeld, A., Hallman, M., Johansson, C. B. & Albrektsson, T. (2010). Histological and histomorphometrical analyses of biopsies harvested 11 years after maxillary sinus floor augmentation with deproteinized bovine and autogenous bone. Clinical Oral Implants Research, 21(9), 961-970
Open this publication in new window or tab >>Histological and histomorphometrical analyses of biopsies harvested 11 years after maxillary sinus floor augmentation with deproteinized bovine and autogenous bone
2010 (English)In: Clinical Oral Implants Research, ISSN 0905-7161, E-ISSN 1600-0501, Vol. 21, no 9, p. 961-970Article in journal (Refereed) Published
Abstract [en]

Objective The purpose of the present study was to histologically and histomorphometrically evaluate the long-term tissue response to deproteinized bovine bone (DPBB) particles used in association with autogenous bone and to compare particle size after 6 months and 11 years, in the same patients, in order to determine possible resorption. Material and methods Twenty consecutive patients (14 women and six men) with a mean age of 62 years (range 48-69 years) with severe atrophy of the posterior maxilla were included in this study. Thirty maxillary sinuses with < 5 mm subantral alveolar bone were augmented with a mixture of 80% DPBB and 20% autogenous bone. Eleven years (mean 11.5 years) after augmentation, biopsies were taken from the grafted areas of the 11 patients who volunteered to participate in this new surgical intervention. The following histomorphometrical measurements were performed in these specimens: total bone area in percentage, total area of the DPBB, total area of marrow space, the degree of DPBB-bone contact (percentage of the total surface length for each particle), the length of all DPBB particles and the area of all DPBB particles. The length and the area of the particles were compared with samples harvested from the same patients at 6 months (nine samples) and pristine particles from the manufacturer. Results The biopsies consisted of 44.7 +/- 16.9% lamellar bone, 38 +/- 16.9% marrow space and 17.3 +/- 13.2% DPBB. The degree of DPBB to bone contact was 61.5 +/- 34%. There were no statistically significant differences between the length and area of the particles after 11 years compared with those measured after 6 months in the same patients or to pristine particles from the manufacturer. Conclusion DPBB particles were found to be well integrated in lamellar bone, after sinus floor augmentation in humans, showing no significant changes in particle size after 11 years.

Keywords
clinical study, histology, bovine hydroxyapatite, autogenous bone graft, fibrin glue, maxillary sinus floor augmentation
National Category
Biomedical Laboratory Science/Technology Medical and Health Sciences
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-12903 (URN)10.1111/j.1600-0501.2010.01939.x (DOI)000280630200012 ()
Available from: 2011-01-04 Created: 2011-01-03 Last updated: 2018-08-28Bibliographically approved
Sarve, H., Lindblad, J., Johansson, C. B. & Borgefors, G. (2010). Methods for visualization of bone tissue in the proximity of implants. In: Leonard Bolc, Ryszard Tadeusiewicz, Leszek J. Chmielewski, Konrad Wojciechowski (Ed.), Computer vision and graphics: Proceedings, Part II. Paper presented at International Conference, ICCVG 2010, September 20-22, Warsaw, Poland, (pp. 243-250). Berlin: Springer
Open this publication in new window or tab >>Methods for visualization of bone tissue in the proximity of implants
2010 (English)In: Computer vision and graphics: Proceedings, Part II / [ed] Leonard Bolc, Ryszard Tadeusiewicz, Leszek J. Chmielewski, Konrad Wojciechowski, Berlin: Springer , 2010, p. 243-250Conference paper, Published paper (Refereed)
Abstract [en]

In this work we present two methods for visualization of SRμCT-scanned 3D volumes of screw-shaped bone implant samples: thread fly-through and 2D unfolding. The thread fly-through generates an animation by following the thread helix and extracting slices along it. Relevant features, such as bone ratio and bone implant contact, are computed for each slice of the animation and displayed as graphs beside the animation. The 2D unfolding, on the other hand, maps the implant surface onto which feature information is projected to a 2D image, providing an instant overview of the whole implant. The unfolding is made area-preserving when appropriate. These visualization methods facilitate better understanding of the bone-implant integration and provides a good platform for communication between involved experts.

Place, publisher, year, edition, pages
Berlin: Springer, 2010
Series
Lecture Notes in Computer Science ; 6375
National Category
Dentistry Dentistry
Research subject
Surface Biotechnology; Medicine
Identifiers
urn:nbn:se:oru:diva-11367 (URN)10.1007/978-3-642-15907-7_30 (DOI)978-3-642-15906-0 (ISBN)
Conference
International Conference, ICCVG 2010, September 20-22, Warsaw, Poland,
Available from: 2010-07-06 Created: 2010-07-06 Last updated: 2017-10-18Bibliographically approved
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