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Bornehag, Carl-GustafORCID iD iconorcid.org/0000-0003-0417-1686
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Publications (9 of 9) Show all publications
Shu, H., Jönsson, B. A., Gennings, C., Svensson, Å., Nånberg, E., Lindh, C. H., . . . Bornehag, C.-G. (2018). Temporal Trends of Phthalate Exposures during 2007-2010 in Swedish Pregnant Women. Journal of Exposure Science and Environmental Epidemiology, 28, 437-447
Open this publication in new window or tab >>Temporal Trends of Phthalate Exposures during 2007-2010 in Swedish Pregnant Women
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2018 (English)In: Journal of Exposure Science and Environmental Epidemiology, ISSN 1559-0631, E-ISSN 1559-064X, Vol. 28, p. 437-447Article in journal (Refereed) Published
Abstract [en]

Background: The general population is exposed to phthalates, a group of chemicals with strong evidence for endocrine disrupting properties, commonly used in a large number of consumer products. Based on published research and evidence compiled by environmental agencies, certain phthalate applications and products have become restricted, leading to an increasing number of “new generation compounds” coming onto the market during recent years replacing older phthalates. Some examples of such newer compounds are di-iso-nonyl phthalate (DiNP), di-iso-decyl phthalate (DiDP), and most recently di-isononyl-cyclohexane-1,2-dicarboxylate (DiNCH).

Objectives: In order to evaluate temporal trends in phthalate exposure, first trimester urinary biomarkers of phthalates were measured in the Swedish SELMA study over a period of 2.5 years (2007–2010).

Methods: We collected first morning void urine samples around week 10 of pregnancy from 1651 pregnant women. Spot samples were analyzed for 13 phthalate metabolites and one phthalate replacement and least square geometric mean (LSGM) levels of the metabolites were compared between the sampling years when adjusted for potential confounders.

Results: All 14 metabolites were detectable in more than 99% of the SELMA subjects. The levels were generally comparable to other studies, but the SELMA subjects showed slightly higher exposure to butyl-benzyl phthalate (BBzP) and di-butyl phthalate (DBP). Di-ethyl-hexyl phthalate (DEHP) metabolites levels decreased while DiNP, DiDP/di-2-propylheptyl phthalate (DPHP), and DiNCH metabolites levels increased during the sampling period.

Conclusions: Urinary metabolite levels of the older phthalates and more recently introduced phthalate replacement compound changed during the short sampling period in this Swedish pregnancy cohort. Our results indicate that replacement of phthalates can make an impact on human exposure to these chemicals. During this particularly vulnerable stage of life, phthalate exposures are of particular concern as the impacts, though not immediately noticeable, may increase the risk for health effects later in life.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
Keywords
DiNCH, Endocrine disrupting chemicals, Exposure, Phthalates, Pregnant, SELMA-Study, Temporal
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:oru:diva-79062 (URN)10.1038/s41370-018-0020-6 (DOI)000444446100003 ()29472621 (PubMedID)2-s2.0-85042354826 (Scopus ID)
Note

The study was funded by grants from the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), Swedish Asthma and Allergy Association’s Research Foundation, the Swedish Foundation for Health Care Sciences and Allergy Research and the County Council of Värmland.

Available from: 2017-08-11 Created: 2020-01-17 Last updated: 2020-01-28Bibliographically approved
Rendel, F., Fjaeraa Alfredsson, C., Bornehag, C.-G., Sundström, B. E. & Nånberg, E. (2017). Effects of Di-Isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. Basic & Clinical Pharmacology & Toxicology, 120(3), 218-323
Open this publication in new window or tab >>Effects of Di-Isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells
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2017 (English)In: Basic & Clinical Pharmacology & Toxicology, ISSN 1742-7835, E-ISSN 1742-7843, Vol. 120, no 3, p. 218-323Article in journal (Refereed) Published
Abstract [en]

Neuropeptide Y (NPY) is an abundant neuropeptide in the mammalian brain important for behavioural consequences of stress and energy metabolism. We have addressed possible effects of the phthalate DiNP on NPY expression in human SH‐SY5Y cells, a neuronal in vitro differentiation model. Pico‐ to nanomolar doses of DiNP and its metabolite MiNP resulted in decreased NPY mRNA and peptide expression in retinoid‐differentiated cells. Thus, dys‐regulated NPY may be an adverse outcome for exposure to low doses of DiNP in human beings.

Place, publisher, year, edition, pages
Blackwell Publishing, 2017
Keywords
phthlate, DiNP, MiNP, NPY, SH-SY5Y neuroblastoma cells
National Category
Medical and Health Sciences Cell and Molecular Biology
Research subject
Biomedical Sciences; Biomedical Sciences
Identifiers
urn:nbn:se:oru:diva-79055 (URN)10.1111/bcpt.12670 (DOI)000394538600016 ()27625336 (PubMedID)2-s2.0-85009476073 (Scopus ID)
Note

This work was financially supported by The County Council of Värmland.

Retraction

The above article, published online on 13 September 2016 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor in Chief, Kim Brøsen, the Nordic Association for the Publication of BCPT and John Wiley & Sons Ltd. The retraction has been agreed following an investigation into the accuracy of the data acquisition conducted by Karlstad University into the accuracy of the data acquisition, which concluded that manipulation had taken place during tests performed on an ELISA equipment in order to achieve a preferred result.

Available from: 2016-09-05 Created: 2020-01-17 Last updated: 2020-02-03Bibliographically approved
Ma, P., Liu, X., Wu, J., Yan, B., Zhang, Y., Lu, Y., . . . Yang, X. (2015). Cognitive deficits and anxiety induced by diisononyl phthalate in mice and the neuroprotective effects of melatonin. Scientific Reports, 5, Article ID 14676.
Open this publication in new window or tab >>Cognitive deficits and anxiety induced by diisononyl phthalate in mice and the neuroprotective effects of melatonin
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2015 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 5, article id 14676Article in journal (Refereed) Published
Abstract [en]

Diisononyl phthalate (DINP) is a plasticizer that is frequently used as a substitute for other plasticizers whose use is prohibited in certain products. In vivo studies on the neurotoxicity of DINP are however, limited. This work aims to investigate whether DINP causes neurobehavioral changes in mice and to provide useful advice on preventing the occurrence of these adverse effects. Behavioral analysis showed that oral administration of 20 or 200â mg/kg/day DINP led to mouse cognitive deficits and anxiety. Brain histopathological observations, immunohistochemistry assays (cysteine-aspartic acid protease 3 [caspase-3], glial fibrillary acidic protein [GFAP]), oxidative stress assessments (reactive oxygen species [ROS], glutathione [GSH], superoxide dismutase [SOD] activities, 8-hydroxy-2-deoxyguanosine [8-OH-dG] and DNA-protein crosslinks [DPC]), and assessment of inflammation (tumor necrosis factor alpha [TNF-Crossed D sign°[ and interleukin-1 beta [IL-1β]) of mouse brains showed that there were histopathological alterations in the brain and increased levels of oxidative stress, and inflammation for these same groups. However, some of these effects were blocked by administration of melatonin (50â mg/kg/day). Down-regulation of oxidative stress was proposed to explain the neuroprotective effects of melatonin. The data suggests that DINP could cause cognitive deficits and anxiety in mice, and that melatonin could be used to avoid these adverse effects.

Place, publisher, year, edition, pages
Nature Publishing Group, 2015
Keywords
Immunohistochemistry, Risk factors
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:oru:diva-79038 (URN)10.1038/srep14676 (DOI)000362087500001 ()26424168 (PubMedID)2-s2.0-84942938338 (Scopus ID)
Note

This work was supported by the Hubei Province Natural Science Foundation Project (2014CFB284), Key Project of National Natural Science Foundation of China (51136002), China Key Technologies R&D Program (2012BAJ02B03) and Hubei Natural Science Foundation Project for Outstanding Young Talents (2012FFA005).

Available from: 2016-06-07 Created: 2020-01-17 Last updated: 2020-01-28Bibliographically approved
Tang, J., Yuan, Y., Wei, C., Liao, X., Yuan, J., Nånberg, E., . . . Yang, X. (2015). Neurobehavioral changes induced by di(2-ethylhexyl) phthalate and the protective effects of vitamin E in Kunming mice. Toxicology Research, 4(4), 1006-1015
Open this publication in new window or tab >>Neurobehavioral changes induced by di(2-ethylhexyl) phthalate and the protective effects of vitamin E in Kunming mice
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2015 (English)In: Toxicology Research, ISSN 2045-452X, E-ISSN 2045-4538, Vol. 4, no 4, p. 1006-1015Article in journal (Refereed) Published
Abstract [en]

Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer commonly used in PVC that may leach into the environment, and has been shown to adversely affect the health of humans and animals. We undertook a study to ascertain the neurotoxicity of DEHP in Kunming mice. This study included three rounds of testing. In the first round, Kunming mice were exposed to different concentrations of DEHP (0, 5, 50, 500 mg kg(-1) per day) after which their cognitive ability was assessed using the Morris water maze (MWM) test. The reactive oxygen species (ROS) content in tissue and the malondialdehyde (MDA) content of brains were also measured. In the second round, vitamin E (50 mg kg(-1) per day) was given daily as an anti-oxidant via the intragastric route. Cognitive deficits and locomotor activity, as well as ROS and MDA contents were tested employing the same methods. In the third round, the depressive mood of mice after DEHP exposure (500 mg kg(-1) per day) was measured using the open field test, the tail suspension test, and the forced swim test. The main findings of this study include: (1) a statistical association exists between DEHP oral exposure and spatial learning (DEHP 500 mg kg(-1) per day) and memory (DEHP 50 mg kg(-1) per day) dysfunction as ascertained by an MWM test of Kunming mice. (2) A statistical association was also found between DEHP oral exposure (50 and 500 mg kg(-1) per day) and oxidative stress (ROS and MDA) of mouse brain tissue. (3) Co-administration of vitamin E (50 mg kg(-1) per day) diminishes the elevation of ROS and MDA induced by DEHP (50 mg kg(-1) per day) from significant levels to non-significant levels. (4) Co-administration of vitamin E (50 mg kg(-1) per day) protects against mouse memory dysfunction induced by DEHP (50 mg kg(-1) per day) from being significant to being not significant. (5) In the 5 mg kg(-1) per day DEHP exposure groups, oxidative stress in brain tissue, and neurobehavioral changes were not found. (6) High dose DEHP exposure (500 mg kg(-1) per day) may induce behavioral despair in mice. Conclusions: These data suggest that DEHP is neurotoxic with regard to cognitive ability and locomotor activity.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2015
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:oru:diva-79069 (URN)10.1039/c4tx00250d (DOI)000356612300023 ()2-s2.0-84934946445 (Scopus ID)
Note

This work was funded by the Key Project of International Cooperation of the Chinese Ministry of Science and Technology (2010DFA31790) and the Key Project of the National Natural Science Foundation of China (51136002).

Available from: 2016-04-11 Created: 2020-01-17 Last updated: 2020-01-28Bibliographically approved
Shu, H., Jönsson, B. A., Larsson, M., Nånberg, E. & Bornehag, C.-G. (2014). PVC flooring at home and development of asthma among young children in Sweden, a 10-year follow-up. Indoor Air, 24(3), 227-235
Open this publication in new window or tab >>PVC flooring at home and development of asthma among young children in Sweden, a 10-year follow-up
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2014 (English)In: Indoor Air, ISSN 0905-6947, E-ISSN 1600-0668, Vol. 24, no 3, p. 227-235Article in journal (Refereed) Published
Abstract [en]

The incidence of asthma and allergy has increased throughout the developed world over the past decades. During the same period of time, the use of industrial chemicals such as phthalates, commonly used as plasticizers in polyvinylchloride (PVC) flooring material, has increased. The aim of this study was to investigate whether PVC flooring in the home of children in the age of 1–5 years is associated with the development of asthma in 5‐ and 10‐year follow‐up investigations (n = 3228). Dampness in Buildings and Health Study (DBH Study) commenced in 2000 in Värmland, Sweden. The current analyses included subjects who answered all baseline and follow‐up questionnaires. Logistic regression analyses were applied to questionnaire results. Children who had PVC floorings in the bedroom at baseline were more likely to develop doctor‐diagnosed asthma during the following 10‐year period when compared with children living without. There were indications that PVC flooring in the parents' bedrooms was strongly associated with the new cases of doctor‐diagnosed asthma when compared with child′s bedroom. Our results suggest that PVC flooring exposure during pregnancy could be a critical period in the development of asthma in children at a later time; prenatal exposure and measurements of phthalate metabolites should be included in the future.

Place, publisher, year, edition, pages
Blackwell Publishing, 2014
Keywords
Allergy, Asthma, Children, Dampness in Buildings and Health Study, Endocrine-disrupting chemicals, Incidence, Longitudinal, Phthalates, Polyvinylchloride flooring
National Category
Medical and Health Sciences Occupational Health and Environmental Health
Research subject
Public Health Science; Biomedical Sciences
Identifiers
urn:nbn:se:oru:diva-79061 (URN)10.1111/ina.12074 (DOI)000335008300002 ()24118287 (PubMedID)2-s2.0-84899651719 (Scopus ID)
Funder
Swedish Research Council Formas
Note

The study was funded by grants from the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (Formas), Swedish Asthma and Allergy Association's Research Foundation, the Swedish Foundation for Health Care Sciences and Allergy Research, and the County Council of Värmland.

Available from: 2013-10-17 Created: 2020-01-17 Last updated: 2020-01-22Bibliographically approved
Shu, H., Jönsson, B. A. .., Lindh, C. H., Knutz, M., Nånberg, E., Svensson, Å. & Bornehag, C. G. (2014). PVC flooring in the home is related to urinary levels of phthalates in swedish pregnant women in the SELMA study. In: Proceedings, Indoor Air 2014, Hong Kong: . Paper presented at Conference of 13th International Conference on Indoor Air Quality and Climate (Indoor Air 2014), Hong Kong, July 7-12 2014. International Society of Indoor Air Quality and Climate
Open this publication in new window or tab >>PVC flooring in the home is related to urinary levels of phthalates in swedish pregnant women in the SELMA study
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2014 (English)In: Proceedings, Indoor Air 2014, Hong Kong, International Society of Indoor Air Quality and Climate , 2014Conference paper, Published paper (Refereed)
Place, publisher, year, edition, pages
International Society of Indoor Air Quality and Climate, 2014
Keywords
Endocrine disruptors; Phthalate; Pregnant; PVC; SELMA-Study
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:oru:diva-79063 (URN)2-s2.0-84924706203 (Scopus ID)
Conference
Conference of 13th International Conference on Indoor Air Quality and Climate (Indoor Air 2014), Hong Kong, July 7-12 2014
Available from: 2016-06-30 Created: 2020-01-17 Last updated: 2020-02-03Bibliographically approved
Bölling, A., Holme, J., Bornehag, C.-G., Nygaard, U. C., Bertelsen, R., Nånberg, E., . . . Becher, R. (2013). Pulmonary phthalate exposure and asthma - is PPAR a plausible mechanistic link?. EXCLI Journal, 12, 733-759
Open this publication in new window or tab >>Pulmonary phthalate exposure and asthma - is PPAR a plausible mechanistic link?
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2013 (English)In: EXCLI Journal, ISSN 1611-2156, E-ISSN 1611-2156, Vol. 12, p. 733-759Article in journal (Refereed) Published
Abstract [en]

Due to their extensive use as plasticisers in numerous consumer products, phthalates have become ubiquitous environmental contaminants. An increasing number of epidemiological studies suggest that exposure to phthalates may be associated with worsening or development of airway diseases. Peroxisome Proliferation Activated Receptors (PPAR)s, identified as important targets for phthalates in early studies in rodent liver, have been suggested as a possible mechanistic link. In this review we discuss the likelihood of an involvement of PPARs in asthma development and exacerbation due to pulmonary phthalate exposure. First, we go through the literature on indoor air levels of phthalates and pulmonary phthalate kinetics. These data are then used to estimate the pulmonary phthalate levels due to inhalation exposure. Secondly, the literature on phthalate-induced activation or modulation of PPARs is summarized. Based on these data, we discuss whether pulmonary phthalate exposure is likely to cause PPAR activation, and if this is a plausible mechanism for adverse effects of phthalates in the lung. It is concluded that the pulmonary concentrations of some phthalates may be sufficient to cause a direct activation of PPARs. Since PPARs mainly mediate antiinflammatory effects in the lungs, a direct activation is not a likely molecular mechanism for adverse effects of phthalates. However, possible modulatory effects of phthalates on PPARs deserve further investigation, including partial antagonist effects and/or cross talk with other signalling pathways. Moreover other mechanisms, including interactions between phthalates and other receptors, could also contribute to possible adverse pulmonary effects of phthalates.

Place, publisher, year, edition, pages
Universität Mainz, 2013
Keywords
Phthalates, asthma, Peroxisome Proliferation Activated Receptors, molecular mechanism
National Category
Public Health, Global Health, Social Medicine and Epidemiology
Research subject
Public Health Science
Identifiers
urn:nbn:se:oru:diva-79020 (URN)000323213400001 ()26622216 (PubMedID)2-s2.0-84882703318 (Scopus ID)
Funder
The Research Council of Norway
Available from: 2016-06-30 Created: 2020-01-20 Last updated: 2020-01-22Bibliographically approved
Guo, J., Han, B., Qin, L., Li, B., You, H., Yang, J., . . . Yang, X. (2012). Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice. PLoS ONE, 7(6), Article ID e39008.
Open this publication in new window or tab >>Pulmonary toxicity and adjuvant effect of di-(2-exylhexyl) phthalate in ovalbumin-immunized BALB/c mice
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2012 (English)In: PLoS ONE, E-ISSN 1932-6203, Vol. 7, no 6, article id e39008Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Asthma is a complex pulmonary inflammatory disease, which is characterized by airway hyperresponsiveness, variable airflow obstruction and inflammation in the airways. The majority of asthma is allergic asthma, which is a disease caused by type I hypersensitivity mediated by IgE. Exposures to a number of environmental chemicals are suspected to lead to asthma, one such pollutant is di-(2-ethylheyl) phthalate (DEHP). DEHP is a manufactured chemical that is commonly added in plastic products to make them flexible. Epidemiological studies have revealed a positive association between DEHP exposure and asthma prevalence.

METHODOLOGY/PRINCIPAL FINDINGS: The present study was aimed to determine the underlying role of DEHP exposure in airway reactivity, especially when combined with allergen exposure. The biomarkers include pulmonary histopathology, airway hyperresponsiveness (lung function), IgE, IL-4, IFN-γ and eosinophils. Healthy balb/c mice were randomly divided into eight exposure groups (n = 8 each): (1) saline control, (2) 30 µg/(kg•d) DEHP, (3) 300 µg/(kg•d) DEHP, (4) 3000 µg/(kg•d) DEHP, and (5) ovalbumin (OVA)-sensitized group, (6) OVA-combined with 30 µg/(kg•d) DEHP, (7) OVA-combined with 300 µg/(kg•d) DEHP, and (8) OVA-combined with 3000 µg/(kg•d) DEHP. Experimental tests were conducted after 52-day DEHP exposure and subsequently one week of challenge with aerosolized OVA. The principal findings include: (1) Strong postive associations exist between OVA-combined DEHP exposure and serum total IgE (T-IgE), as well as histological findings. These positive associations show a dose-dependent low dose sensitive effect of DEHP. (2) IL-4, eosinophil recruitment and lung function are also indicators for adjuvant effect of DEHP.

CONCLUSIONS/SIGNIFICANCE: Our results suggest that except the significant changes of immunological and inflammatory biomarkers (T-IgE, IL-4, IFN-γ and eosinophils), the pulmonary histological (histopathological examination) and physiological (lung function) data also support that DEHP may promote and aggravate allergic asthma by adjuvant effect.

Place, publisher, year, edition, pages
PLOS, 2012
National Category
Respiratory Medicine and Allergy
Research subject
Biomedical Science
Identifiers
urn:nbn:se:oru:diva-79032 (URN)10.1371/journal.pone.0039008 (DOI)000305340000059 ()22701742 (PubMedID)2-s2.0-84862224204 (Scopus ID)
Note

This work was supported by the China National Natural Science Foundation (grant ID: 51136002, 51076079) and National Key Technologies R&D Program of China (grant ID: 2012BAJ02B03). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Available from: 2013-10-17 Created: 2020-01-17 Last updated: 2020-01-28Bibliographically approved
Bornehag, C.-G. & Nanberg, E. (2010). Phthalate exposure and asthma in children. International Journal of Andrology, 33(2), 333-345
Open this publication in new window or tab >>Phthalate exposure and asthma in children
2010 (English)In: International Journal of Andrology, ISSN 0105-6263, E-ISSN 1365-2605, Vol. 33, no 2, p. 333-345Article in journal (Refereed) Published
Abstract [en]

During the last decades more than 100 000 new chemicals have been introduced to the environment. Many of these new chemicals and many common consumer products that include these have been shown to be toxic in animal studies and an increasing body of evidence suggests that they are also impacting human health. Among the suspect chemicals, the endocrine disrupting chemicals (EDCs) are of particular concern. One such chemical group is the phthalates, used in soft poly vinyl chloride (PVC) material and in a huge number of consumer products. During the same period of time that the prevalence of these modern chemicals has increased, there has been a remarkable increase in several chronic illnesses, including asthma and allergy in children. In this article we outline the scientific knowledge on phthalate exposure for asthma and airway diseases in children by examining epidemiological and experimental peer review data for potential explanatory mechanisms. Epidemiological data point to a possible correlation between phthalate exposure and asthma and airway diseases in children. Experimental studies present support for an adjuvant effect on basic mechanisms in allergic sensitization by several phthalates. Despite variations in the experimental design and reported result in the individual studies, a majority of published reports have identified adjuvant effects on Th2 differentiation, production of Th2 cytokines and enhanced levels of Th2 promoted immunoglobulins (mainly IgG1 but also IgE) in mice. A limited amount of data do also suggest phthalate-induced enhancement of mast cell degranulation and eosinophilic infiltration which are important parts in the early inflammation phase. Thus, some of the early key mechanisms in the pathology of allergic asthma could possibly be targeted by phthalate exposure. But the important questions of clinical relevance of real life exposure and identification of molecular targets that can explain interactions largely remain to be answered

Place, publisher, year, edition, pages
Blackwell Publishing, 2010
Keywords
asthma, endocrine disrupters, environmental factors, epidemiology, immunology
National Category
Biological Sciences Respiratory Medicine and Allergy
Research subject
Biomedical Science
Identifiers
urn:nbn:se:oru:diva-79042 (URN)10.1111/j.1365-2605.2009.01023.x (DOI)000275636400017 ()20059582 (PubMedID)2-s2.0-77949572961 (Scopus ID)
Available from: 2012-02-08 Created: 2020-01-17 Last updated: 2020-01-28Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0003-0417-1686

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