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Carstens, Adam
Publications (5 of 5) Show all publications
Carstens, A., Dicksved, J., Nelson, R., Lindqvist, C. M., Andreasson, A., Bohr, J., . . . Halfvarson, J. (2019). The Gut Microbiota in Collagenous Colitis Shares Characteristics With Inflammatory Bowel Disease-Associated Dysbiosis. Clinical and Translational Gastroenterology, 10(7), Article ID e00065.
Open this publication in new window or tab >>The Gut Microbiota in Collagenous Colitis Shares Characteristics With Inflammatory Bowel Disease-Associated Dysbiosis
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2019 (English)In: Clinical and Translational Gastroenterology, ISSN 2155-384X, E-ISSN 2155-384X, Vol. 10, no 7, article id e00065Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: In inflammatory bowel disease (IBD), an aberrant immune response to gut microbiota is important, but the role of the microbiota in collagenous colitis (CC) is largely unknown. We aimed to characterize the microbiota of patients with CC compared with that of healthy control and patients with IBD.

METHODS: Fecal samples were collected from patients with CC (n = 29), age- and sex-matched healthy controls (n = 29), patients with Crohn's disease (n = 32), and patients with ulcerative colitis (n = 32). Sequence data were obtained by 454 sequencing of 16S rRNA gene amplicons, and the obtained sequences were subsequently taxonomically classified.

RESULTS: Analysis of similarity statistics showed a segregation between patients with CC and healthy controls with increasing taxonomic resolution, becoming significant comparing operational taxonomic unit data (P = 0.006). CC had a lower abundance of 10 different taxa. Taxa-specific analyses revealed a consistent lower abundance of several operational taxonomic units belonging to the Ruminococcaceae family in patients with CC, q < 0.05 after false discovery rate correction. Loss of these taxa was seen in patients with CC with active disease and/or corticosteroid treatment only and resembled the findings in patients with IBD.

DISCUSSION: CC is associated with a specific fecal microbiome seen primarily in patients with active disease or ongoing corticosteroid treatment, whereas the microbiome of CC patients in remission resembled that of healthy controls. Notably, the shift in key taxa, including the Ruminococcaceae family, was also observed in IBD. There may be common mechanisms in the pathogenesis of CC and IBD.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-75573 (URN)10.14309/ctg.0000000000000065 (DOI)000478837900001 ()31343467 (PubMedID)
Available from: 2019-08-09 Created: 2019-08-09 Last updated: 2019-08-16Bibliographically approved
Carstens, A., Roos, A., Andreasson, A., Magnuson, A., Agréus, L., Halfvarson, J. & Engstrand, L. (2018). Differential clustering of faecal and mucosa-associated microbiota in healthy individuals. Journal of Digestive Diseases, 19(12), 745-752
Open this publication in new window or tab >>Differential clustering of faecal and mucosa-associated microbiota in healthy individuals
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2018 (English)In: Journal of Digestive Diseases, ISSN 1751-2972, E-ISSN 1751-2980, Vol. 19, no 12, p. 745-752Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Faecal samples are often used to characterise gut microbiota, since they are easily collected. However, whether or not the faecal microbiota differ from the mucosa-associated microbiota remains largely unknown. This may be specifically relevant in conditions that are characterised by complex mucosal microbe-host interactions, such as Crohn's disease. We aimed to determine the degree of agreement between faecal and mucosal microbiota profiles in healthy individuals, using two commonly used collection procedures.

MATERIAL AND METHODS: The gut microbiota composition of faecal samples (sent at ambient temperature before storage at -70°C) and of colonic biopsies (obtained at endoscopy and immediately stored at -70°C) was determined by sequencing the 16S rRNA gene. Thirty-one randomly selected healthy individuals from the population-based colonoscopy (Popcol) study were included.

RESULTS: Faecal samples were characterised by a reduced degree of richness (p<0.0001) and diversity (p=0.016), and also differences in several phyla, including a lower relative abundance of Proteobacteria (p<0.0001) and Verrucomicrobia (p=0.008) than in biopsies. Only 3 of 30 individuals had a similar faecal and mucosal microbiota profile, based on weighted UniFrac analysis. A difference in Crohn's disease dysbiosis-associated bacteria was observed, including a lower relative abundance of Faecalibacterium (p=0.004) and a higher relative abundance of Ruminococcus (p=0.001) in faeces than in biopsies.

CONCLUSIONS: Analysis of faecal samples that have been transported at ambient temperature does not adequately reflect the colonic mucosa-associated microbiota in healthy individuals. These findings have implications for the interpretation of the previous literature, and may be specifically relevant to studies on Crohn's disease.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Asia, 2018
Keywords
Crohn disease, fecal microbiota, mucosa-associated microbiota
National Category
Microbiology in the medical area Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-70345 (URN)10.1111/1751-2980.12688 (DOI)000455494100004 ()30467977 (PubMedID)2-s2.0-85059893806 (Scopus ID)
Funder
Swedish Research Council, 2012-1930 2011-2764
Note

Funding Agencies:

Örebro University  

Bengt Ihre Foundation  

Nanna Svartz Foundation  

Örebro University Hospital Research Foundation  

Örebro County Research Foundation  

Söderberg Foundation  

Swedish Foundation for Gastrointestinal Research 

Available from: 2018-11-27 Created: 2018-11-27 Last updated: 2019-01-29Bibliographically approved
Vatn, S., Karlsson, M. C., Carstens, A., Detlie, T. E., Ricanek, P., Bergemalm, D., . . . Vatn, M. H. (2018). Faecal microbiota in newly diagnosed Crohn's disease and its relation to treatment escalation. Journal of Crohn's & Colitis, 12(Suppl. 1), S555-S555
Open this publication in new window or tab >>Faecal microbiota in newly diagnosed Crohn's disease and its relation to treatment escalation
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2018 (English)In: Journal of Crohn's & Colitis, ISSN 1873-9946, E-ISSN 1876-4479, Vol. 12, no Suppl. 1, p. S555-S555Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Oxford University Press, 2018
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-66758 (URN)000427318902249 ()
Available from: 2018-04-26 Created: 2018-04-26 Last updated: 2018-09-12Bibliographically approved
Vatn, S., Karlsson, M. C., Carstens, A., Detlie, T. E., Ricanek, P., Lindquist, C. M., . . . Vatn, M. H. (2018). Faecal microbiota in treatment-naive ulcerative colitis and its relation to treatment escalation. Journal of Crohn's & Colitis, 12(Suppl. 1), S557-S557
Open this publication in new window or tab >>Faecal microbiota in treatment-naive ulcerative colitis and its relation to treatment escalation
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2018 (English)In: Journal of Crohn's & Colitis, ISSN 1873-9946, E-ISSN 1876-4479, Vol. 12, no Suppl. 1, p. S557-S557Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Oxford University Press, 2018
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-66757 (URN)000427318902253 ()
Available from: 2018-04-26 Created: 2018-04-26 Last updated: 2018-09-12Bibliographically approved
Ricanek, P., Rahul, K., Ber, Y., Vatn, S., Finnby, L., Lindahl, T., . . . Vatn, M. H. (2017). Microbiota related disease activity and distribution in subgroups of inflammatory bowel disease. Journal of Crohn's & Colitis, 11(Suppl. 1), S483-S484
Open this publication in new window or tab >>Microbiota related disease activity and distribution in subgroups of inflammatory bowel disease
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2017 (English)In: Journal of Crohn's & Colitis, ISSN 1873-9946, E-ISSN 1876-4479, Vol. 11, no Suppl. 1, p. S483-S484Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Oxford University Press, 2017
National Category
Gastroenterology and Hepatology
Identifiers
urn:nbn:se:oru:diva-57786 (URN)10.1093/ecco-jcc/jjx002.909 (DOI)000398606901319 ()28175257 (PubMedID)
Available from: 2017-05-23 Created: 2017-05-23 Last updated: 2018-07-31Bibliographically approved
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