oru.sePublications
Change search
Link to record
Permanent link

Direct link
BETA
Siekmann, Wiebke
Publications (4 of 4) Show all publications
Siekmann, W., Tina, E., Koskela von Sydow, A. & Gupta, A. (2019). Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines. Oncology Letters, 18(1), 395-401
Open this publication in new window or tab >>Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines
2019 (English)In: Oncology Letters, ISSN 1792-1074, E-ISSN 1792-1082, Vol. 18, no 1, p. 395-401Article in journal (Refereed) Published
Abstract [en]

Regional anesthesia may prolong survival following surgery for different types of cancers. The mechanisms behind this are unclear but direct effects on cancer cells by local anesthetics (LA) have been suggested. The aim of this study was to investigate if lidocaine or ropivacaine have a dose-dependent effect on the cell viability and proliferation of a primary and a secondary colon carcinoma cell line in vitro. The colon cancer cell lines SW480 derived from primary tumor and SW620 from a metastatic site in the same patient were exposed to increasing concentrations of lidocaine and ropivacaine (5-1,000 mu M). Cell viability was measured using CellTiter-Blue((R)) and cell proliferation by PKH67 after exposure for up to 72 h. Cell viability was significantly reduced by ropivacaine at the highest concentration (1,000 mu M) after 48 and 72 h in the cell line SW480 and at 72 h in SW620. Exposure to lidocaine did not show any significant reduction in cell viability. Notably, low concentrations of both lidocaine and ropivacaine significantly increased cell viability after 48 and 72 h in SW620. Cell proliferation was significantly reduced by 1,000 mu M lidocaine in SW480 and by 1,000 mu M ropivacaine in SW620. In summary, both lidocaine and ropivacaine showed an anti-proliferative effect in the colon cancer cell lines at high concentrations and after prolonged exposure to LA in vitro. Our findings also indicate that lower concentrations promote cell viability in the metastatic cell line.

Place, publisher, year, edition, pages
Spandidos Publications, 2019
Keywords
local anesthetics, colon cancer, SW480, SW620, in vitro, cell viability, proliferation
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:oru:diva-75408 (URN)10.3892/ol.2019.10332 (DOI)000474896900047 ()2-s2.0-85068799738 (Scopus ID)
Note

Funding Agency:

Research Committee of the Örebro County Council

Available from: 2019-07-30 Created: 2019-07-30 Last updated: 2019-07-30Bibliographically approved
Forget, P., Aguirre, J. A., Bencic, I., Borgeat, A., Cama, A., Condron, C., . . . Buggy, D. (2019). How Anesthetic, Analgesic and Other Non-Surgical Techniques During Cancer Surgery Might Affect Postoperative Oncologic Outcomes: A Summary of Current State of Evidence. Cancers, 11(5), Article ID 592.
Open this publication in new window or tab >>How Anesthetic, Analgesic and Other Non-Surgical Techniques During Cancer Surgery Might Affect Postoperative Oncologic Outcomes: A Summary of Current State of Evidence
Show others...
2019 (English)In: Cancers, ISSN 2072-6694, Vol. 11, no 5, article id 592Article, review/survey (Refereed) Published
Abstract [en]

The question of whether anesthetic, analgesic or other perioperative intervention during cancer resection surgery might influence long-term oncologic outcomes has generated much attention over the past 13 years. A wealth of experimental and observational clinical data have been published, but the results of prospective, randomized clinical trials are awaited. The European Union supports a pan-European network of researchers, clinicians and industry partners engaged in this question (COST Action 15204: Euro-Periscope). In this narrative review, members of the Euro-Periscope network briefly summarize the current state of evidence pertaining to the potential effects of the most commonly deployed anesthetic and analgesic techniques and other non-surgical interventions during cancer resection surgery on tumor recurrence or metastasis.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
cancer, anesthesia, analgesia
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:oru:diva-75264 (URN)10.3390/cancers11050592 (DOI)000472738300005 ()31035321 (PubMedID)2-s2.0-85068567385 (Scopus ID)
Available from: 2019-07-24 Created: 2019-07-24 Last updated: 2019-07-24Bibliographically approved
Siekmann, W., Tina, E. & Gupta, A. (2017). Concentration-dependent cell viability and proliferation in vitro of colon cancer cell lines SW480 and SW620 on exposure to lidocaine or ropivacaine. Acta Anaesthesiologica Scandinavica, 61(8), 1017-1018
Open this publication in new window or tab >>Concentration-dependent cell viability and proliferation in vitro of colon cancer cell lines SW480 and SW620 on exposure to lidocaine or ropivacaine
2017 (English)In: Acta Anaesthesiologica Scandinavica, ISSN 0001-5172, E-ISSN 1399-6576, Vol. 61, no 8, p. 1017-1018Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Background: Cancer cells change phenotypes and properties when evolving from primary tumor cells to metastatic cells. These changes might affect the response to local anesthetics (LA). The aim of this study was to investigate if lidocaine or ropivacaine have a dose- dependent effect on cell viability and proliferation of a primary and a secondary colon carcinoma cell line in vitro.

Methods: The colon cancer cell lines SW 480, derived from primary tumor and SW620 from metastatic tumor in the same patient, were exposed to increasing log-concentrations of lidocaine and ropivacaine. Cell viability was measured using CellTiter Blue, and cell proliferation by PKH67, after exposure for up to 72 h.

Results: Cell viability was not affected after 24 h of exposure. However, the metastatic cell line SW620 showed a significant increase in cell viability at low concentrati ons after 48 and 72 h. Exposure to the higher, but clinically relevant, concentrations of both LA resulted in decreased cell viability in both cell lines. These higher concentrations also showed an inhibitory effect on cell proliferation after 72 h, which was more pronounced for ropivacaine.

Conclusions: Low concentrations of lidocaine and ropivacaine, as achieved in plasma by epidural infusion of LA, do not have direct antiproliferative effects on these colon cancer cell lines in vitro. Higher concentrations of LA, as during continuous local infiltration into tissues over 72 h, inhibit proliferation of both cancer cell lines. The increase in cell viability seen in SW620 should be investigated and underlying mechanisms further elucidated in future studies.

Place, publisher, year, edition, pages
John Wiley & Sons, 2017
National Category
Anesthesiology and Intensive Care
Identifiers
urn:nbn:se:oru:diva-59281 (URN)10.1111/aas.12941 (DOI)000407231100098 ()
Available from: 2017-08-29 Created: 2017-08-29 Last updated: 2018-08-05Bibliographically approved
Siekmann, W., Eintrei, C., Magnuson, A., Sjölander, A., Matthiessen, P., Myrelid, P. & Gupta, A. (2017). Surgical and not analgesic technique affects postoperative inflammation following colorectal cancer surgery: a prospective, randomized study. Colorectal Disease, 19(6), O186-O195
Open this publication in new window or tab >>Surgical and not analgesic technique affects postoperative inflammation following colorectal cancer surgery: a prospective, randomized study
Show others...
2017 (English)In: Colorectal Disease, ISSN 1462-8910, E-ISSN 1463-1318, Vol. 19, no 6, p. O186-O195Article in journal (Refereed) Published
Abstract [en]

AIM: Epidural analgesia reduces the surgical stress response. However, its effect on pro- and anti-inflammatory cytokines in the genesis of inflammation following major abdominal surgery remains unclear. Our main objective was to elucidate whether perioperative epidural analgesia prevents the inflammatory response following colorectal cancer surgery.

METHODS: 96 patients scheduled for open or laparoscopic surgery were randomized to epidural analgesia (group E) or patient controlled intravenous analgesia (group P). Surgery and anaesthesia were standardized in both groups. Plasma cortisol, insulin and serum cytokines (IL-1β,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12p70,IL-13,TNFα,IFNγ,GM-CSF,PGE2 and VEGF) were measured preoperatively (T0), 1-6 hours postoperatively (T1) and 3-5 days postoperatively (T2). Mixed model analysis was used, after logarithmic transformation when appropriate, for analyses of cytokines and stress markers.

RESULTS: There were no significant differences in any serum cytokine concentration between groups P and E at any time point except in IL-10 which was 87% higher in group P (median and range 4.1 (2.3-9.2) pg/ml,) compared to group E (2.6 (1.3-4.7) pg/ml) (p=0.002) at T1. There was no difference in plasma cortisol and insulin between the groups at any time point after surgery. Significant difference in median serum cytokine concentration was found between open and laparoscopic surgery with higher levels of IL-6,IL-8 and IL-10 at T1 in patients undergoing open surgery compared to laparoscopic surgery. No difference in serum cytokine concentration was detected between the groups or between the surgical technique at T2.

CONCLUSIONS: Open surgery, compared to laparoscopic surgery, has greater impact on these inflammatory mediators than epidural analgesia vs. intravenous analgesia. This article is protected by copyright. All rights reserved.

Place, publisher, year, edition, pages
John Wiley & Sons, 2017
Keywords
Anaesthesia, epidural, surgery, colorectal cancer, inflammation, cytokines
National Category
Anesthesiology and Intensive Care Surgery
Research subject
Oncology; Surgery; Anaesthesiology
Identifiers
urn:nbn:se:oru:diva-57387 (URN)10.1111/codi.13643 (DOI)000402674400003 ()28258664 (PubMedID)2-s2.0-85018758511 (Scopus ID)
Note

Funding Agency:

Regional Research Committee, Örebro-Uppsala Region, Sweden

Available from: 2017-05-03 Created: 2017-05-03 Last updated: 2018-09-18Bibliographically approved
Organisations

Search in DiVA

Show all publications