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Tarum, Janelle
Publications (2 of 2) Show all publications
Alexopoulou, S., Fart, F., Jonsson, A.-S., Karni, L., Kenalemang, L. M., Krishna, S., . . . Widell, B. (2018). Successful ageing in an interdisciplinary context: popular science presentations. Örebro: Örebro University
Open this publication in new window or tab >>Successful ageing in an interdisciplinary context: popular science presentations
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2018 (English)Book (Other (popular science, discussion, etc.))
Place, publisher, year, edition, pages
Örebro: Örebro University, 2018. p. 127
National Category
Gerontology, specialising in Medical and Health Sciences Other Social Sciences not elsewhere specified
Identifiers
urn:nbn:se:oru:diva-66306 (URN)978-91-87789-18-2 (ISBN)
Available from: 2018-04-03 Created: 2018-04-03 Last updated: 2018-09-14Bibliographically approved
Tarum, J., Folkesson, M., Atherton, P. J. & Kadi, F. (2017). Electrical pulse stimulation: an in vitro exercise model for the induction of human skeletal muscle cell hypertrophy. A proof-of-concept study. Experimental Physiology, 102(11), 1405-1413
Open this publication in new window or tab >>Electrical pulse stimulation: an in vitro exercise model for the induction of human skeletal muscle cell hypertrophy. A proof-of-concept study
2017 (English)In: Experimental Physiology, ISSN 0958-0670, E-ISSN 1469-445X, Vol. 102, no 11, p. 1405-1413Article in journal (Refereed) Published
Abstract [en]

New Findings:

  • What is the central question of this study?

Is electrical pulse stimulation (EPS) an in vitro exercise model able to elicit the hypertrophy of human muscle cells?

  • What is the main finding and its importance?

The addition of a restitution period of 8h after EPS induces the enlargement of human muscle cells, a major physiological end-point to resistance exercise. This is supported by downregulationof myostatin, a negative regulator of muscle mass, and increased phosphorylated mTOR and 4E-BP1, key factors in the growth cascade. This proof-of-concept study provides a model of physiologically mediated muscle growth, which will be the basis for future studies aiming to depict molecular events governing the hypertrophy of human muscle cells.

Electrical pulse stimulation (EPS) of muscle cells has previouslybeenused as an in vitro exercise model. The present study aimedto establish an EPS protocol promoting the hypertrophy ofhuman muscle cells, which represents a major physiological end-point to resistance exercise in humans. We hypothesized that adding a resting period after EPS would be crucial for the occurrence of the morphological change. Myoblasts obtained from human muscle biopsies (n=5) were differentiated into multinucleated myotubes and exposed to 8h of EPS consisting of 2ms pulses at 12V, with a frequency of 1Hz. Myotube size was assessed using immunohistochemistry immediately, 4 and 8h after completed EPS. Gene expression and phosphorylation status of selected markers of hypertrophy were assessed using RT-PCR and Western blotting, respectively. Release of the myokine interleukin-6 in culture medium was measured using enzyme-linked immunosorbent assay. We demonstrated a significant increase (31 +/- 14%; P=0.03) in the size of myotubes when EPS was followed by an 8h resting period, but not immediately or 4h after completion of EPS. The response was supported by downregulation (P=0.04) of the gene expression of myostatin, a negative regulator of muscle mass, and an increase in phosphorylated mTOR (P=0.03) and 4E-BP1 (P=0.01), which are important factors in the cellular growth signalling cascade. The present work demonstrates that EPS is an in vitro exercise model promoting the hypertrophy of human muscle cells, recapitulating a major physiological end-point to resistance exercise in human skeletal muscle.

Place, publisher, year, edition, pages
John Wiley & Sons, 2017
Keywords
Cell growth, muscle contraction, myotube morphology
National Category
Physiology
Identifiers
urn:nbn:se:oru:diva-61685 (URN)10.1113/EP086581 (DOI)000414175100010 ()28861930 (PubMedID)2-s2.0-85032974682 (Scopus ID)
Available from: 2017-11-14 Created: 2017-11-14 Last updated: 2018-09-24Bibliographically approved
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