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Mandal, Abul, ProfessorORCID iD iconorcid.org/0000-0002-6319-4055
Publications (10 of 12) Show all publications
Nahar, N., Rahman, A., Ghosh, S., Nawani, N. & Mandal, A. (2017). Functional studies of AtACR2 gene putatively involved in accumulation, reduction and/or sequestration of arsenic species in plants. Biologia (Bratislava), 72(5), 520-526
Open this publication in new window or tab >>Functional studies of AtACR2 gene putatively involved in accumulation, reduction and/or sequestration of arsenic species in plants
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2017 (English)In: Biologia (Bratislava), ISSN 0006-3088, E-ISSN 1336-9563, Vol. 72, no 5, p. 520-526Article in journal (Refereed) Published
Abstract [en]

Food-based exposure to arsenic is a human carcinogen and can severely impact human health resulting in many cancerous diseases and various neurological and vascular disorders. This project is a part of our attempts to develop new varieties of crops for avoiding arsenic contaminated foods. For this purpose, we have previously identified four key genes, and molecular functions of two of these, AtACR2 and AtPCSl, have been studied based on both in silico and in vivo experiments. In the present study, a T-DNA tagged mutant, (SALK-143282C with mutation in AtACR2 gene) of Arabidopsis thaliana was studied for further verification of the function of AtACR2 gene. Semi-quantitative RT-PCR analyses revealed that this mutant exhibits a significantly reduced expression of the AtACR2 gene. When exposed to 100 μM of arsenate (AsV) for three weeks, the mutant plants accumulated arsenic approximately three times higher (778 μg/g d. wt.) than that observed in the control plants (235 μg/g d. wt.). In contrast, when the plants were exposed to 100 μM of arsenite (AsIII), no significant difference in arsenic accumulation was observed between the control and the mutant plants (535 μg/g d. wt. and 498 μg/g d. wt., respectively). Also, when arsenate and arsenite was measured separately either in shoots or roots, significant differences in accumulation of these substances were observed between the mutant and the control plants. These results suggest that AtACR2 gene is involved not only in accumulation of arsenic in plants, but also in conversion of arsenate to arsenite inside the plant cells. © 2017 Institute of Molecular Biology, Slovak Academy of Sciences.

Place, publisher, year, edition, pages
Walter de Gruyter, 2017
Keywords
Arabidopsis thaliana, arsenate reductase 2 gene, arsenic accumulation, arsenic speciation, IC-ICP-DRC-MS, RT-PCR
National Category
Biochemistry and Molecular Biology Botany
Research subject
Biotechnology
Identifiers
urn:nbn:se:oru:diva-70464 (URN)10.1515/biolog-2017-0062 (DOI)000404241300006 ()2-s2.0-85021444188 (Scopus ID)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved
Rahman, A., Olsson, B., Jass, J., Nawani, N., Ghosh, S. & Mandal, A. (2017). Genome Sequencing Revealed Chromium and Other Heavy Metal Resistance Genes in E. cloacae B2-Dha. Journal of Microbial & Biochemical Technology, 9(5), 191-199
Open this publication in new window or tab >>Genome Sequencing Revealed Chromium and Other Heavy Metal Resistance Genes in E. cloacae B2-Dha
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2017 (English)In: Journal of Microbial & Biochemical Technology, E-ISSN 1948-5948, Vol. 9, no 5, p. 191-199Article in journal (Refereed) Published
Abstract [en]

The previously described chromium resistant bacterium, Enterobacter cloacae B2-DHA, was isolated from leather manufacturing tannery landfill in Bangladesh. Here we report the entire genome sequence of this bacterium containing chromium and other heavy metal resistance genes. The genome size and the number of genes, determined by massive parallel sequencing and comparative analysis with other known Enterobacter genomes, are predicted to be 4.22 Mb and 3958, respectively. Nearly 160 of these genes were found to be involved in binding, transport, and catabolism of ions as well as efflux of inorganic and organic compounds. Specifically, the presence of two chromium resistance genes, chrR and chrA was verified by polymerase chain reaction. The outcome of this research highlights the significance of this bacterium in bioremediation of chromium and other toxic metals from the contaminated sources.

Place, publisher, year, edition, pages
Omics Publishing Group, 2017
Keywords
Genome Sequencing, Bioremediation, Toxic metals, Enterobacter cloacae, Gene annotation
National Category
Bioinformatics and Systems Biology
Research subject
Biotechnology; Bioinformatics; INF501 Integration of -omics Data
Identifiers
urn:nbn:se:oru:diva-70477 (URN)10.4172/1948-5948.1000365 (DOI)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-04Bibliographically approved
Rahman, A., Nahar, N., Nawani, N. N. & Mandal, A. (2017). Investigation on Arsenic-Accumulating and Arsenic-Transforming Bacteria for Potential Use in the Bioremediation of Arsenics. In: Surajit Das, Hirak Ranjan Dash (Ed.), Handbook of Metal-Microbe Interactions and Bioremediation: (pp. 509-520). Boca Raton, FL: CRC Press
Open this publication in new window or tab >>Investigation on Arsenic-Accumulating and Arsenic-Transforming Bacteria for Potential Use in the Bioremediation of Arsenics
2017 (English)In: Handbook of Metal-Microbe Interactions and Bioremediation / [ed] Surajit Das, Hirak Ranjan Dash, Boca Raton, FL: CRC Press, 2017, p. 509-520Chapter in book (Refereed)
Abstract [en]

In this chapter, arsenic-accumulating and arsenic- transformingbacterial strains that can be employed as a sourcefor cost-effective and eco-friendly bioremediation of arsenicsfrom contaminated environments have been reviewed. Thischapter demonstrates that many naturally occurring bacterialstrains like B1-CDA have the potential for reducing arseniccontent in contaminated sources to safe levels. Therefore,the socioeconomic impact of this kind of microorganisms ishighly significant for those countries, especially in the developingworld, where impoverished families and villages aremost impacted. Therefore, this discovery should be consideredto be the most significant factor in formulating nationalstrategies for effective poverty elimination. Besides humanarsenic contamination, these bacterial strains will also benefitlivestock and native animal species, and the outcome ofthese studies is vital not only for people in arsenic-affectedareas but also for human populations in other countries thathave credible health concerns as a consequence of arseniccontaminatedwater and foods.

Place, publisher, year, edition, pages
Boca Raton, FL: CRC Press, 2017
National Category
Microbiology
Research subject
Biotechnology
Identifiers
urn:nbn:se:oru:diva-70474 (URN)10.1201/9781315153353 (DOI)9781498762427 (ISBN)9781498762434 (ISBN)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved
Nahar, N., Rahman, A., Nawani, N. N., Ghosh, S. & Mandal, A. (2017). Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana. Journal of plant physiology (Print), 218, 121-126
Open this publication in new window or tab >>Phytoremediation of arsenic from the contaminated soil using transgenic tobacco plants expressing ACR2 gene of Arabidopsis thaliana
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2017 (English)In: Journal of plant physiology (Print), ISSN 0176-1617, E-ISSN 1618-1328, Vol. 218, p. 121-126Article in journal (Refereed) Published
Abstract [en]

We have cloned, characterized and transformed the AtACR2 gene (arsenic reductase 2) of Arabidopsis thaliana into the genome of tobacco (Nicotiana tabacum, var Sumsun). Our results revealed that the transgenic tobacco plants are more tolerant to arsenic than the wild type ones. These plants can grow on culture medium containing 200μM arsenate, whereas the wild type can barely survive under this condition. Furthermore, when exposed to 100μM arsenate for 35days the amount of arsenic accumulated in the shoots of transgenic plants was significantly lower (28μg/g d wt.) than that found in the shoots of non-transgenic controls (40μg/g d wt.). However, the arsenic content in the roots of transgenic plants was significantly higher (2400μg/g d. wt.) than that (2100μg/g d. wt.) observed in roots of wild type plants. We have demonstrated that Arabidopsis thaliana AtACR2 gene is a potential candidate for genetic engineering of plants to develop new crop cultivars that can be grown on arsenic contaminated fields to reduce arsenic content of the soil and can become a source of food containing no arsenic or exhibiting substantially reduced amount of this metalloid.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Arabidopsis thaliana, Arsenic, AtACR2 overexpression, Heavy metal accumulation, Nicotiana tabacum, Phytoremediation
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biotechnology
Identifiers
urn:nbn:se:oru:diva-70463 (URN)10.1016/j.jplph.2017.08.001 (DOI)000413327800014 ()28818758 (PubMedID)2-s2.0-85031780264 (Scopus ID)
Funder
Sida - Swedish International Development Cooperation Agency, AKT-2010-018Swedish Research Council Formas, 229-2007-217
Note

Funding Agency:

Nilsson-Ehle Foundation (The Royal Physiographic Society in Lund) in Sweden

Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved
Yewale, P. P., Rahman, A., Nahar, N., Saha, A., Jass, J., Mandal, A. & Nawani, N. N. (2017). Sources of Metal Pollution, Global Status, and Conventional Bioremediation Practices. In: Surajit Das, Hirak Ranjan Dash (Ed.), Handbook of Metal–Microbe Interactions and Bioremediation: (pp. 25-40). Boca Raton, FL: CRC Press
Open this publication in new window or tab >>Sources of Metal Pollution, Global Status, and Conventional Bioremediation Practices
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2017 (English)In: Handbook of Metal–Microbe Interactions and Bioremediation / [ed] Surajit Das, Hirak Ranjan Dash, Boca Raton, FL: CRC Press, 2017, p. 25-40Chapter in book (Refereed)
Abstract [en]

Pollution control has become a priority task for global regulatory authorities. The framing of regulations, guidelines, and implementation of pollution awareness and control programs has begun at a massive scale. Heavy metals that are one of the most challenging pollutants that affect humans, animals, plants, and the ecosystem health. The sources of different metals and their toxicities are described. Current approaches in bioremediation are addressed along with the challenges posed by them. Furthermore, recent developments in biotechnology that offer novel ways to recover metals from contaminated sites are discussed.

Place, publisher, year, edition, pages
Boca Raton, FL: CRC Press, 2017
Keywords
Metal Pollution, Bioremediation, Human Health, Microbial Biotechnology
National Category
Microbiology
Research subject
Biotechnology
Identifiers
urn:nbn:se:oru:diva-70478 (URN)10.1201/9781315153353 (DOI)9781498762427 (ISBN)9781498762434 (ISBN)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved
Rahman, A., Nahar, N., Olsson, B. & Mandal, A. (2016). Complete Genome Sequence of Enterobacter cloacae B2-DHA: a Chromium-Resistant Bacterium. Genome Announcements, 4(3), Article ID e00483-16.
Open this publication in new window or tab >>Complete Genome Sequence of Enterobacter cloacae B2-DHA: a Chromium-Resistant Bacterium
2016 (English)In: Genome Announcements, ISSN 2169-8287, E-ISSN 2169-8287, Vol. 4, no 3, article id e00483-16Article in journal (Refereed) Published
Abstract [en]

Previously, we reported a chromium-resistant bacterium, Enterobacter cloacae B2-DHA, isolated from the landfills of tannery industries in Bangladesh. Here, we investigated its genetic composition using massively parallel sequencing and comparative analysis with other known Enterobacter genomes. Assembly of the sequencing reads revealed a genome of ~4.21 Mb in size.

Place, publisher, year, edition, pages
American Society for Microbiology, 2016
Keywords
Enterobacter cloacae, Genome sequencing, de novo assembly, Gene annotation
National Category
Bioinformatics and Systems Biology
Research subject
Bioinformatics; Biotechnology
Identifiers
urn:nbn:se:oru:diva-70476 (URN)10.1128/genomeA.00483-16 (DOI)27257201 (PubMedID)2-s2.0-85009965114 (Scopus ID)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-04Bibliographically approved
Rahman, A., Nahar, N., Jass, J., Olsson, B. & Mandal, A. (2016). Complete genome sequence of Lysinibacillus sphaericus B1-CDA: a bacterium that accumulates arsenics. Genome Announcements, 4(1), Article ID e00999-15.
Open this publication in new window or tab >>Complete genome sequence of Lysinibacillus sphaericus B1-CDA: a bacterium that accumulates arsenics
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2016 (English)In: Genome Announcements, ISSN 2169-8287, E-ISSN 2169-8287, Vol. 4, no 1, article id e00999-15Article in journal (Refereed) Published
Abstract [en]

Here, we report the genomic sequence and genetic composition of an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA. Assembly of the sequencing reads revealed that the genome size is ~4.5 Mb encompassing ~80% of the chromosomal DNA.

Place, publisher, year, edition, pages
American Society for Microbiolology, 2016
National Category
Bioinformatics and Systems Biology
Research subject
Natural sciences; Bioinformatics
Identifiers
urn:nbn:se:oru:diva-70469 (URN)10.1128/genomeA.00999-15 (DOI)26798084 (PubMedID)2-s2.0-85009977094 (Scopus ID)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-04Bibliographically approved
Nawani, N., Rahman, A., Nahar, N., Saha, A., Kapadnis, B. & Mandal, A. (2016). Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora. Biologia (Bratislava), 71(5), 494-507
Open this publication in new window or tab >>Status of metal pollution in rivers flowing through urban settlements at Pune and its effect on resident microflora
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2016 (English)In: Biologia (Bratislava), ISSN 0006-3088, E-ISSN 1336-9563, Vol. 71, no 5, p. 494-507Article in journal (Refereed) Published
Abstract [en]

This study illustrates the sporadic distribution of metals in fluvial systems flowing from catchments to urban settlements. This is a detailed study prognosticating the deteriorating quality of rivers at specific locations due to metal pollution. Heavy metals like cadmium, lead, nickel and mercury are prominent in industrial sector. Contour plots derived using spatial and temporal data could determine the focal point of metal pollution and its gradation. Metal values recorded were cadmium 157 mg/L, lead 47 mg/L, nickel 61 mg/L and mercury 0.56 mg/L. Prokaryote diversity was less in polluted water and it harboured metal tolerant bacteria, which were isolated from these polluted sites. Actinomycetes like Streptomyces and several other bacteria like Stenotrophomonas and Pseudomonas isolated from the polluted river sites exhibited changes in morphology in presence of heavy metals. This stress response offered remedial measures as Streptomyces were effective in biosorption of cadmium, nickel and lead and Stenotrophomonas and Pseudomonas were effective in the bioaccumulation of lead and cadmium. The amount of 89 mg of lead and 106 mg of nickel could be adsorbed on one gram of Streptomyces biomass-based biosorbent. Such biological remedies can be further explored to remove metals from polluted sites and from metal contaminated industrial or waste waters.

Place, publisher, year, edition, pages
Walter de Gruyter, 2016
Keywords
Metal pollution, Bioremediation, Morphological Changes, Pune rivers, Prokaryote Diversity
National Category
Microbiology Environmental Sciences Water Engineering
Research subject
Natural sciences
Identifiers
urn:nbn:se:oru:diva-70465 (URN)10.1515/biolog-2016-0074 (DOI)000379818000005 ()2-s2.0-84976553017 (Scopus ID)
Projects
Bioremediation of toxic metals and other pollutants for protecting human health and the ecosystem
Funder
Sida - Swedish International Development Cooperation Agency
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved
Islam, M. S., Mohanto, N. C., Karim, M. R., Aktar, S., Hoque, M. M., Rahman, A., . . . Hossain, K. (2015). Elevated concentrations of serum matrix metalloproteinase-2 and -9 and their associations with circulating markers of cardiovascular diseases in chronic arsenic-exposed individuals. Environmental health, 14(1), Article ID 92.
Open this publication in new window or tab >>Elevated concentrations of serum matrix metalloproteinase-2 and -9 and their associations with circulating markers of cardiovascular diseases in chronic arsenic-exposed individuals
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2015 (English)In: Environmental health, ISSN 1476-069X, E-ISSN 1476-069X, Vol. 14, no 1, article id 92Article in journal (Refereed) Published
Abstract [en]

Background: Cardiovascular diseases (CVDs) and cancers are the major causes of chronic arsenic exposure-related morbidity and mortality. Matrix metalloproteinase-2 (MMP-2) and −9 (MMP-9) are deeply involved in the pathogenesis of CVDs and cancers. This study has been designed to evaluate the interactions of arsenic exposure with serum MMP-2 and MMP-9 concentrations especially in relation to the circulating biomarkers of CVDs.

Methods: A total of 373 human subjects, 265 from arsenic-endemic and 108 from non-endemic areas in Bangladesh were recruited for this study. Arsenic concentrations in the specimens were measured by inductively coupled plasma mass spectroscopy (ICP-MS) and serum MMPs were quantified by immunoassay kits.

Results: Serum MMP-2 and MMP-9 concentrations in arsenic-endemic population were significantly (p < 0.001) higher than those in non-endemic population. Both MMPs showed significant positive interactions with drinking water (rs = 0.208, p < 0.001 for MMP-2; rs = 0.163, p <0.01 for MMP-9), hair (rs= 0.163, p < 0.01 for MMP-2; rs = 0.173, p < 0.01 for MMP-9) and nail (rs= 0.160, p < 0.01 for MMP-2; rs = 0.182, p < 0.001 for MMP-9) arsenic of the study subjects. MMP-2 concentrations were 1.02, 1.03 and 1.05 times, and MMP-9 concentrations were 1.03, 1.06 and 1.07 times greater for 1 unit increase in log-transformed water, hair and nail arsenic concentrations, respectively, after adjusting for covariates (age, sex, BMI, smoking habit and hypertension). Furthermore, both MMPs were increased dose-dependently when the study subjects were split into three (≤10, 10.1-50 and > 50 μg/L) groups based on the regulatory upper limit of water arsenic concentration set by WHO and Bangladesh Government. MMPs were also found to be significantly (p < 0.05) associated with each other. Finally, the concentrations of both MMPs were correlated with several circulating markers related to CVDs.

Conclusions: This study showed the significant positive associations and dose–response relationships of arsenic exposure with serum MMP-2 and MMP-9 concentrations. This study also showed the interactions of MMP-2 and MMP-9 concentrations with the circulating markers of CVDs suggesting the MMP-2 and MMP-9 -mediated mechanism of arsenic-induced CVDs.

Place, publisher, year, edition, pages
BioMed Central, 2015
Keywords
Arsenic, MMP-2, MMP-9, Cardiovascular diseases, Cancer, Bangladesh
National Category
Biochemistry and Molecular Biology
Research subject
Natural sciences
Identifiers
urn:nbn:se:oru:diva-70459 (URN)10.1186/s12940-015-0079-7 (DOI)000366085600001 ()26637202 (PubMedID)2-s2.0-84949512940 (Scopus ID)
Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-05Bibliographically approved
Nahar, N., Rahman, A., Moś, M., Warzecha, T., Ghosh, S., Hossain, K., . . . Mandal, A. (2014). In silico and in vivo studies of molecular structures and mechanisms of AtPCS1 protein involved in binding arsenite and/or cadmium in plant cells. Journal of Molecular Modeling, 20(3), Article ID 2104.
Open this publication in new window or tab >>In silico and in vivo studies of molecular structures and mechanisms of AtPCS1 protein involved in binding arsenite and/or cadmium in plant cells
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2014 (English)In: Journal of Molecular Modeling, ISSN 1610-2940, E-ISSN 0948-5023, Vol. 20, no 3, article id 2104Article in journal (Refereed) Published
Abstract [en]

This paper reports a continuation of our previous research on the phytochelatin synthase1 (PCS1) gene involved in binding and sequestration of heavy metals or metalloids in plant cells. Construction of a 3D structure of the Arabidopsis thaliana PCS1 protein and prediction of gene function by employing iterative implementation of the threading assembly refinement (I-TASSER) revealed that PC ligands (3GC-gamma-glutamylcysteine) and Gln50, Pro53, Ala54, Tyr55, Cys56, Ile102, Gly161, His162, Phe163, Asp204 and Arg211 residues are essential for formation of chelating complex with cadmium (Cd²⁺) or arsenite (AsIII). This finding suggests that the PCS1 protein might be involved in the production of the enzyme phytochelatin synthase, which might in turn bind, localize, store or sequester heavy metals in plant cells. For validation of the in silico results, we included a T-DNA tagged mutant of Arabidopsis thaliana, SAIL_650_C12, (mutation in AtPCS1 gene) in our investigation. Furthermore, using reverse transcriptase PCR we confirmed that the mutant does not express the AtPCS1 gene. Mutant plants of SAIL_650_C12 were exposed to various amounts of cadmium (Cd²⁺) and arsenite (AsIII) and the accumulation of these toxic metals in the plant cells was quantified spectrophotometrically. The levels of Cd²⁺ and AsIII accumulation in the mutant were approximately 2.8 and 1.6 times higher, respectively, than that observed in the wild-type controlled plants. We confirmed that the results obtained in in silico analyses complement those obtained in in vivo experiments.

Place, publisher, year, edition, pages
Springer, 2014
Keywords
Arabidopsis thaliana, Substrate binding residue, Heavy metal, In silico analysis, In vivo experiment, Phytochelatin synthase
National Category
Biochemistry and Molecular Biology Plant Biotechnology
Research subject
Natural sciences
Identifiers
urn:nbn:se:oru:diva-70461 (URN)10.1007/s00894-014-2104-0 (DOI)000332180100010 ()24554125 (PubMedID)2-s2.0-84893828770 (Scopus ID)
Projects
Bioremediation
Funder
Sida - Swedish International Development Cooperation Agency, AKT-2010-018Swedish Research Council Formas, 229-2007-217
Note

Funding agency:

The Nilsson-Ehle Foundation (The Royal Physiographic Society in Lund)

Available from: 2018-12-04 Created: 2018-12-04 Last updated: 2018-12-06Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-6319-4055

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