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Qian, Minjie
Publications (3 of 3) Show all publications
Rodriguez-Calzada, T., Qian, M., Strid, Å., Neugart, S., Schreiner, M., Torres-Pacheco, I. & Guevara-Gonzales, R. (2019). Effect of UV-B radiation on morphology, phenolic compound production, gene expression, and subsequent drought stress responses in chili pepper (Capsicum annuum L.). Plant physiology and biochemistry (Paris), 134, 94-102
Open this publication in new window or tab >>Effect of UV-B radiation on morphology, phenolic compound production, gene expression, and subsequent drought stress responses in chili pepper (Capsicum annuum L.)
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2019 (English)In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 134, p. 94-102Article in journal (Refereed) Published
Abstract [en]

It has been suggested that accumulation of flavonoids could be a key step in development of plant tolerance to different environmental stresses. Moreover, it has been recognized that abiotic stresses such as drought and UV-B radiation (280-315 nm) induce phenolic compound accumulation, suggesting a role for these compounds in drought tolerance. The aim of the present study was to evaluate the effect of UV-B exposure on chili pepper (Capsicum annuum, cv. ‘Coronel’) plant performance, phenolic compound production, and gene expression associated with response to subsequent drought stress. Additionally, the phenotypic response to drought stress of these plants was studied. UV-B induced a reduction both in stem length, stem dry weight and number of floral primordia. The largest reduction in these variables was observed when combining UV-B and drought. UV-B-treated well-watered plants displayed fructification approximately 1 week earlier than non-UV-B-treated controls. Flavonoids measured epidermally in leaves significantly increased during UV-B treatment. Specifically, UV-B radiation significantly increased chlorogenic acid and apigenin 8-C-hexoside levels in leaves and a synergistic increase of luteolin 6-C-pentoside-8-C-hexoside was obtained by UV-B and subsequent drought stress. Gene expression of phenylalanine ammonia lyase (PAL) and chalcone synthase (CHS) genes also increased during UV-B treatments. On the other hand, expression of genes related to an oxidative response, such as mitochondrial Mn-superoxide dismutase (Mn-SOD) and peroxidase (POD) was not induced by UV-B. Drought stress in UV-B-treated plants induced mitochondrial Mn-SOD gene expression. Taken together, the UV-B treatment did not induce significant tolerance in plants towards drought stress under the conditions used.

Place, publisher, year, edition, pages
Paris, France: Elsevier, 2019
Keywords
Drought, Dualex, Flavonoids, Jalapeno pepper, Morphology, Ultraviolet-B radiation
National Category
Botany Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-67300 (URN)10.1016/j.plaphy.2018.06.025 (DOI)000455692000010 ()29950274 (PubMedID)2-s2.0-85048888625 (Scopus ID)
Funder
Swedish Research Council Formas, 942 - 2015-516Knowledge Foundation, 20130164
Note

Funding Agencies:

CONACYT  283259

Faculty for Business, Science and Technology at Örebro University

Available from: 2018-06-19 Created: 2018-06-19 Last updated: 2019-01-29Bibliographically approved
Ni, J., Bai, S., Zhao, Y., Qian, M., Tao, R., Yin, L., . . . Teng, Y. (2019). Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in 'Red Zaosu' pear fruits by interacting with MYB114. Plant Molecular Biology, 99(1-2), 67-78
Open this publication in new window or tab >>Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in 'Red Zaosu' pear fruits by interacting with MYB114
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2019 (English)In: Plant Molecular Biology, ISSN 0167-4412, E-ISSN 1573-5028, Vol. 99, no 1-2, p. 67-78Article in journal (Refereed) Published
Abstract [en]

KEY MESSAGE: Pp4ERF24 and Pp12ERF96 fine tune blue light-induced anthocyanin biosynthesis via interacting with PpMYB114 and promoting the interaction between PpMYB114 and PpbHLH3, which enhances the expression of PpMYB114-induced PpUFGT.

The red coloration of pear fruit is attributed to anthocyanin accumulation, which is transcriptionally regulated by the MYB-bHLH-WD40 complex. A number of ethylene response factors (ERF) have been identified to regulate anthocyanin biosynthesis in different plants. In pear, several ERF transcription factor genes were identified to be potentially involved in the light-induced anthocyanin biosynthesis according to transcriptome data. But the molecular mechanism of these ERFs underlying the regulation of anthocyanin accumulation is unknown. In this study, exposure of 'Red Zaosu' pear, a mutant of 'Zaosu' pear, to blue light significantly induced the anthocyanin accumulation by increasing the expression levels of anthocyanin biosynthetic genes. Gene expression analysis confirmed that the expression of Pp4ERF24 and Pp12ERF96 genes were up-regulated in the process of blue light-induced anthocyanin biosynthesis. Yeast two-hybrid and bimolecular fluorescence complementation assay revealed that Pp4ERF24 and Pp12ERF96 interacted with PpMYB114, but not with PpMYB10. Bimolecular fluorescence complementation assay demonstrated that the interaction between these two ERFs and PpMYB114 enhanced the interaction between PpMYB114 and PpbHLH3. Further analysis by dual luciferase assay verified that these two ERFs increased the up-regulation of PpMYB114-mediated PpUFGT expression. Furthermore, co-transformation of Pp12ERF96 with PpMYB114 and PpbHLH3 in tobacco leaves led to enhanced anthocyanin accumulation. Transient overexpression of Pp4ERF24 or Pp12ERF96 alone in 'Red Zaosu' pear fruit also induced anthocyanin biosynthesis in pear peel. Our findings provide insights into a mechanism involving the synergistic interaction of ERFs with PpMYB114 to regulate light-dependent coloration and anthocyanin biosynthesis in pear fruits.

Place, publisher, year, edition, pages
Springer, 2019
Keywords
Anthocyanin, Blue light, ERF, MYB, Pear (Pyrus)
National Category
Chemical Sciences Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:oru:diva-70807 (URN)10.1007/s11103-018-0802-1 (DOI)000457944100005 ()30539403 (PubMedID)2-s2.0-85058304743 (Scopus ID)
Note

Funding Agencies:

National Natural Science Foundation of China  31471852  31772272 

Earmarked Fund for China Agriculture Research System  CARS-28 

Available from: 2018-12-18 Created: 2018-12-18 Last updated: 2019-02-19Bibliographically approved
Qian, M., Kalbina, I., Rosenqvist, E., Jansen, M. A. K., Teng, Y. & Strid, Å. (2019). UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner. Photochemical and Photobiological Sciences, 18(2), 424-433
Open this publication in new window or tab >>UV regulates expression of phenylpropanoid biosynthesis genes in cucumber (Cucumis sativus L.) in an organ and spectrum dependent manner
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2019 (English)In: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 18, no 2, p. 424-433Article in journal (Refereed) Published
Abstract [en]

Expression of cucumber (Cucumis sativus) genes encoding the phenylpropanoid and flavonoid biosynthetic enzymes phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H), and chalcone synthase (CHS), was studied under control light conditions (photosynthetically active radiation, PAR) in root, stem, and leaf. Furthermore, expression was quantified in leaves illuminated with PAR and supplemental ultraviolet-A (315-400nm) or ultraviolet-B (280-315 nm) radiation. The expression pattern of all twelve CsPAL, threeCsC4H, and three CsCHS genes was established. Among the genes regulated by UV two general expression patterns emerge. One pattern applies to genes primarily regulated by enriched UV-A illumination (pattern 1). Another (pattern 2) was found for the genes regulated by enriched UV-B. Three of the pattern 2 genes (CsPAL4, CsPAL10, CsCHS2) displayed a particular sub-pattern (pattern 2b) with transcription enriched by at least 30 fold. In contrast to the other genes studied, the promoters of the genes regulated according to pattern 2b contained a combination of a number of cis-acting regulatory elements (MREs, ACEs, and G-boxes) that may be of importance for the particularly high enhancement of expression under UV-B- containing light. The regulation of phenylpropanoid and flavonoid biosynthesis genes in cucumber resembles that of a number of other plants. However, cucumber, due to its greater size, is an attractive species for more detailed studies of the fine regulation of spatial and temporal expression of key genes. This in turn, can facilitate the quantitative investigation of the relationships between different promotor motifs, the expression levels of each of these three genes, and metabolite accumulation profiles.

Place, publisher, year, edition, pages
London, UK: Royal Society of Chemistry, 2019
National Category
Biochemistry and Molecular Biology Botany
Research subject
Biochemistry
Identifiers
urn:nbn:se:oru:diva-70117 (URN)10.1039/C8PP00480C (DOI)000458569100015 ()30628617 (PubMedID)2-s2.0-85061384256 (Scopus ID)
Funder
Swedish Research Council Formas, 942-2015-516Knowledge Foundation, 20130164The Swedish Foundation for International Cooperation in Research and Higher Education (STINT), CH2016-6788
Note

Funding Agencies:

Orebro University's Faculty for Business, Science and Technology  

Science Foundation Ireland  SFI 16-IA-4418 

National Natural Science Foundation of China  31711530027 

China Scholarship Council (CSC) 

Available from: 2018-11-11 Created: 2018-11-11 Last updated: 2019-06-18Bibliographically approved
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