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Alfaro-Moreno, ErnestoORCID iD iconorcid.org/0000-0003-1132-7992
Publications (3 of 3) Show all publications
Alfaro-Moreno, E., Quintana-Belmares, R., Montiel-Davalos, A., Gustafsson, A., Miranda, J., Lopez-Marure, R. & Rosas-Perez, I. (2019). Expression of receptors for adhesion molecules in monocytes exposed to urban particulate matter is independent of size and composition of the particles. Paper presented at 55th Congress of the European-Societies-of-Toxicology (EUROTOX 2019) - Toxicology - Science Providing Solutions, Helsinki, Finland, September 8-11, 2019. Toxicology Letters, 314(Suppl.), S232-S233
Open this publication in new window or tab >>Expression of receptors for adhesion molecules in monocytes exposed to urban particulate matter is independent of size and composition of the particles
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2019 (English)In: Toxicology Letters, ISSN 0378-4274, E-ISSN 1879-3169, Vol. 314, no Suppl., p. S232-S233Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Elsevier, 2019
National Category
Pharmacology and Toxicology Cell Biology
Identifiers
urn:nbn:se:oru:diva-76749 (URN)10.1016/j.toxlet.2019.09.002 (DOI)000484771800626 ()
Conference
55th Congress of the European-Societies-of-Toxicology (EUROTOX 2019) - Toxicology - Science Providing Solutions, Helsinki, Finland, September 8-11, 2019
Available from: 2019-09-26 Created: 2019-09-26 Last updated: 2019-09-26Bibliographically approved
Åkerlund, E., Islam, M. S., McCarrick, S., Alfaro-Moreno, E. & Karlsson, H. L. (2019). Inflammation and (secondary) genotoxicity of Ni and NiO nanoparticles. Nanotoxicology, 13(8), 1060-1072
Open this publication in new window or tab >>Inflammation and (secondary) genotoxicity of Ni and NiO nanoparticles
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2019 (English)In: Nanotoxicology, ISSN 1743-5390, E-ISSN 1743-5404, Vol. 13, no 8, p. 1060-1072Article in journal (Refereed) Published
Abstract [en]

Nanoparticle-induced genotoxicity can arise through different mechanisms, and generally, primary and secondary genotoxicity can be distinguished where the secondary is driven by an inflammatory response. It is, however, yet unclear how a secondary genotoxicity can be detected using in vitro methods. The aim of this study was to investigate inflammation and genotoxicity caused by agglomerated nickel (Ni) and nickel oxide (NiO) nanoparticles and, furthermore, to explore the possibility to test secondary (inflammation-driven) genotoxicity in vitro. As a benchmark particle to compare with, we used crystalline silica (quartz). A proteome profiler antibody array was used to screen for changes in release of 105 different cytokines and the results showed an increased secretion of various cytokines including vascular endothelial growth factor (VEGF) following exposure of macrophages (differentiated THP-1 cells). Both Ni and NiO caused DNA damage (comet assay) following exposure of human bronchial epithelial cells (HBEC) and interestingly conditioned media (CM) from exposed macrophages also resulted in DNA damage (2- and 3-fold increase for Ni and NiO, respectively). Similar results were also found when using a co-culture system of macrophages and epithelial cells. In conclusion, this study shows that it is possible to detect a secondary genotoxicity in lung epithelial cells by using in vitro methods based on conditioned media or co-cultures. Further investigation is needed in order to find out what factors that are causing this secondary genotoxicity and whether such effects are caused by numerous nanoparticles.

Place, publisher, year, edition, pages
Taylor & Francis, 2019
Keywords
Co-culture, nanoparticles, nanomaterials, nickel, secondary genotoxicity
National Category
Cell Biology
Identifiers
urn:nbn:se:oru:diva-75726 (URN)10.1080/17435390.2019.1640908 (DOI)000477282900001 ()31322448 (PubMedID)2-s2.0-85071703787 (Scopus ID)
Funder
Swedish Research Council, 2014-4598Forte, Swedish Research Council for Health, Working Life and Welfare, 2011-0832
Note

Funding Agency:

Swedish Fund for Research Without Animal Experiments  2017-0041

Available from: 2019-08-13 Created: 2019-08-13 Last updated: 2019-10-07Bibliographically approved
Huerta-García, E., Ramos-Godinez, M. D., López-Saavedra, A., Alfaro-Moreno, E., Gómez-Crisóstomo, N. P., Colín-Val, Z., . . . López-Marure, R. (2019). Internalization of Titanium Dioxide Nanoparticles Is Mediated by Actin-Dependent Reorganization and Clathrin- and Dynamin-Mediated Endocytosis in H9c2 Rat Cardiomyoblasts. Chemical Research in Toxicology, 32(4), 578-588
Open this publication in new window or tab >>Internalization of Titanium Dioxide Nanoparticles Is Mediated by Actin-Dependent Reorganization and Clathrin- and Dynamin-Mediated Endocytosis in H9c2 Rat Cardiomyoblasts
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2019 (English)In: Chemical Research in Toxicology, ISSN 0893-228X, E-ISSN 1520-5010, Vol. 32, no 4, p. 578-588Article in journal (Refereed) Published
Abstract [en]

Titanium dioxide nanoparticles (TiO2 NPs) are widely used for industrial and commercial applications. Once inside the body, they translocate into the bloodstream and reach different areas of the cardiovascular system including the heart, increasing the risk of developing cardiovascular diseases; consequently, the investigation of their interaction with cardiac cells is required. We previously showed that TiO2 NPs are internalized by H9c2 rat cardiomyoblasts, and here, we examined the molecular mechanisms underlying this process. TiO2 NPs internalization was evaluated by transmission electron microscopy, time-lapse microscopy, and flow cytometry. Changes in the actin cytoskeleton were studied by phalloidin staining. Endocytic uptake mechanisms for nanoparticles were probed with chemical inhibitors, whereas clathrin and dynamin expression was measured by Western blot. Cellular uptake of TiO2 NPs occurred early after 30 min exposure, and large aggregates were observed after 1 h. Actin cytoskeleton reorganization included cell elongation plus lower density and stability of actin fibers. Cytochalasin-D inhibited TiO2 NPs uptake, indicating actin-mediated internalization. Dynamin and clathrin levels increased early after TiO2 NPs exposure, and their inhibition reduced nanoparticle uptake. Therefore, TiO2 NPs internalization by H9c2 rat cardiomyoblasts involves actin cytoskeleton reorganization and clathrin/dynamin-mediated endocytosis.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
National Category
Cell Biology Pharmacology and Toxicology
Identifiers
urn:nbn:se:oru:diva-72884 (URN)10.1021/acs.chemrestox.8b00284 (DOI)000465190200007 ()30730135 (PubMedID)2-s2.0-85062568137 (Scopus ID)
Note

Funding Agency:

CONACyT  295174  570169

Available from: 2019-03-04 Created: 2019-03-04 Last updated: 2019-06-19Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-1132-7992

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