To Örebro University

oru.seÖrebro University Publications
Change search
Link to record
Permanent link

Direct link
Alternative names
Publications (7 of 7) Show all publications
Hayderi, A., Kumawat, A. K., Shavva, V. S., Dreifaldt, M., Sigvant, B., Petri, M., . . . Ljungberg, L. (2024). RSAD2 is abundant in atherosclerotic plaques and promotes interferon-induced CXCR3-chemokines in human smooth muscle cells. Scientific Reports, 14(1), Article ID 8196.
Open this publication in new window or tab >>RSAD2 is abundant in atherosclerotic plaques and promotes interferon-induced CXCR3-chemokines in human smooth muscle cells
Show others...
2024 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 14, no 1, article id 8196Article in journal (Refereed) Published
Abstract [en]

In atherosclerotic lesions, monocyte-derived macrophages are major source of interferon gamma (IFN-γ), a pleotropic cytokine known to regulate the expression of numerous genes, including the antiviral gene RSAD2. While RSAD2 was reported to be expressed in endothelial cells of human carotid lesions, its significance for the development of atherosclerosis remains utterly unknown. Here, we harnessed publicly available human carotid atherosclerotic data to explore RSAD2 in lesions and employed siRNA-mediated gene-knockdown to investigate its function in IFN-γ-stimulated human aortic smooth muscle cells (hAoSMCs). Silencing RSAD2 in IFN-γ-stimulated hAoSMCs resulted in reduced expression and secretion of key CXCR3-chemokines, CXCL9, CXCL10, and CXCL11. Conditioned medium from RSAD2-deficient hAoSMCs exhibited diminished monocyte attraction in vitro compared to conditioned medium from control cells. Furthermore, RSAD2 transcript was elevated in carotid lesions where it was expressed by several different cell types, including endothelial cells, macrophages and smooth muscle cells. Interestingly, RSAD2 displayed significant correlations with CXCL10 (r =  0.45, p = 0.010) and CXCL11 (r = 0.53, p = 0.002) in human carotid lesions. Combining our findings, we uncover a novel role for RSAD2 in hAoSMCs, which could potentially contribute to monocyte recruitment in the context of atherosclerosis.

Place, publisher, year, edition, pages
Nature Publishing Group, 2024
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:oru:diva-113037 (URN)10.1038/s41598-024-58592-9 (DOI)001198838600052 ()38589444 (PubMedID)2-s2.0-85189798266 (Scopus ID)
Funder
Örebro UniversityKnowledge Foundation, 20180035Stiftelsen Gamla Tjänarinnor, 2020-01074; 2021-01198Stiftelsen Sigurd och Elsa Goljes minne, LA2020-0196Sjukvårdsregionala forskningsrådet Mellansverige, RFR-750481; RFR-940393
Available from: 2024-04-09 Created: 2024-04-09 Last updated: 2024-11-11Bibliographically approved
Kurt, S., Pirronello, F., Reitsema, R., Demirel, I., Rangel, I., Sirsjö, A., . . . Kumawat, A. (2023). Increased proportion of circulating neutrophils with impaired phagocytosis capacity in patients with peripheral arterial disease. Paper presented at 91st Annual Meeting of the European-Atherosclerosis-Society (EAS 2023), Mannheim, Germany, May 21-24, 2023. Atherosclerosis, 379(Suppl. 1), S22-S22, Article ID P068.
Open this publication in new window or tab >>Increased proportion of circulating neutrophils with impaired phagocytosis capacity in patients with peripheral arterial disease
Show others...
2023 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 379, no Suppl. 1, p. S22-S22, article id P068Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Background and Aims: Peripheral arterial disease (PAD) is a clinical manifestation of atherosclerosis, affecting arteries in the leg. Based on their symptoms and severity, PAD patients are characterized into three sub-groups: asymptomatic, intermittent claudication (IC) and critical limb ischemia (CLI). Despite its high prevalence, PAD remains under diagnosed and the role of immune cells in PAD pathophysiology remains poorly understood. In this study, we characterized the innate immune responses in PAD patients compared to healthy controls.

Methods: Blood samples were collected from 14 patients with PAD (IC) and 30 healthy controls, to assess the phenotype of monocytes and neutrophils by using 10-colour flow cytometry. Phagocytosis assay was performed with labelled E.coli particles. Mann-Whitney U non-parametrical test was used for statistical comparison between PAD patients and healthy controls.

Results: A significant higher proportion of leukocytes (p<0.05) and neutrophils (p<0.01) was observed in PAD patients compared to healthy controls, whereas monocyte subsets showed no significant differences. Interestingly, neutrophils showed a significantly impaired phagocytosis capability (p<0.05) and reduced expression of myeloperoxidase (MPO) (p<0.05) in PAD patients compared to healthy controls.

Conclusions: Taken together these results, suggest that PAD patients have an increased proportion of neutrophils in circulation, with impaired phagocytosis capability, compared to healthy controls.

Place, publisher, year, edition, pages
Elsevier, 2023
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:oru:diva-109555 (URN)001060595800354 ()
Conference
91st Annual Meeting of the European-Atherosclerosis-Society (EAS 2023), Mannheim, Germany, May 21-24, 2023
Available from: 2023-11-06 Created: 2023-11-06 Last updated: 2023-11-06Bibliographically approved
Pirronello, F., Kurt, S., Reitsema, R., Rangel, I., Dreifaldt, M., Sirsjö, A. & Kumawat, A. (2023). Phenotypic and functional characterization of T cell immune responses in peripheral arterial disease. Paper presented at 91st Annual Meeting of the European-Atherosclerosis-Society (EAS 2023), Mannheim, Germany, May 21-24, 2023. Atherosclerosis, 379(Suppl. 1), S25-S25, Article ID P076.
Open this publication in new window or tab >>Phenotypic and functional characterization of T cell immune responses in peripheral arterial disease
Show others...
2023 (English)In: Atherosclerosis, ISSN 0021-9150, E-ISSN 1879-1484, Vol. 379, no Suppl. 1, p. S25-S25, article id P076Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Background and Aims: Peripheral arterial disease (PAD) is a common manifestation of atherosclerosis, affecting the lower limbs. T cells are among the principal contributors to the development of atherosclerotic plaques. However, T cell immune responses in PAD pathophysiology are poorly understood and a detailed phenotypic and functional characterization of T cell immune responses in PAD is needed.

Methods: Blood samples were collected from PAD patients with claudicatio intermittens (n=14) and healthy controls (HCs, n=30). We assessed the phenotype of active, effector and memory T cell subsets by evaluating the expression of specific surface and intracellular markers analysed by 10-colour flow cytometry. Functional responses were evaluated by performing T cell receptor (TCR) stimulation of PBMCs in a 3D cell culture system to assess cytokine production by ELISA. Statistical analyses were performed using the Mann-Whitney U test.

Results: No differences were observed between PAD and HCs in terms of active, effector and memory T cell phenotypes and in the frequency of cells expressing CCR6 and CXCR3 (markers associated with T cells producing IL-17 and IFN-γ). However, lower frequencies of IFN-γ+ cells among CD8+ (P=0.04), and CD4+CD8+ cells (P=0.03) were observed in PAD compared to HCs. TNF-α production in PAD-derived PBMCs, via TCR stimulation was increased at both 48- (P=0.004) and 72-hour time points (P=0.003). No differences were observed in IL-1β, IFN-γ and IL-17 secretion.

Conclusions: Taken together these results suggest that increased TNF-α secretion by PBMCs in response to TCR activation might contribute to the pro-inflammatory environment in PAD pathogenesis.

Place, publisher, year, edition, pages
Elsevier, 2023
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:oru:diva-109561 (URN)001060595800362 ()
Conference
91st Annual Meeting of the European-Atherosclerosis-Society (EAS 2023), Mannheim, Germany, May 21-24, 2023
Available from: 2023-11-06 Created: 2023-11-06 Last updated: 2023-11-06Bibliographically approved
Norlander, L., Sundqvist, A.-S., Anderzen-Carlsson, A., Dreifaldt, M., Andreasson, J. & Vidlund, M. (2022). Health-related quality of life after Nuss procedure for pectus excavatum: a cross-sectional study. Interactive Cardiovascular and Thoracic Surgery, 35(1), Article ID ivac031.
Open this publication in new window or tab >>Health-related quality of life after Nuss procedure for pectus excavatum: a cross-sectional study
Show others...
2022 (English)In: Interactive Cardiovascular and Thoracic Surgery, ISSN 1569-9293, E-ISSN 1569-9285, Vol. 35, no 1, article id ivac031Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: Pectus excavatum (PE) can cause both physical and psychosocial symptoms and affect patients' health-related quality of life. Previous international studies have shown that the Nuss procedure increases both self-esteem and body image. The aim of the study was to evaluate the health-related quality of life in patients who have undergone the Nuss procedure for PE.

METHODS: The study had a cross-sectional multicentre design. All patients (N = 420) who underwent the Nuss procedure for PE in 3 cardiothoracic departments in Sweden from 2000 to 2019 were invited to answer the RAND-36 and Nuss Questionnaire modified for adults. Genders were compared using the Mann-Whitney U-test. Patients were divided into groups based on age at time of surgery (<20, 20-30 or >30 years) and analysed by the Kruskal-Wallis H-test with post-hoc analyses.

RESULTS: A total of 236 patients returned the questionnaires; 82.2% were males. Men scored significantly better on the modified Nuss Questionnaire total (P = 0.01) and psychosocial (P = 0.02) subscales. Patients who had surgery at <20 years of age had significantly better scores on the same scales (P = 0.007 and 0.006, respectively) compared to patients aged 20-30 years at the time of surgery. However, no significant difference was seen in comparison with patients >30 years. Patients who had their bar removed had significantly better values on both scales.

CONCLUSIONS: Male gender, young age at surgery and bar removal seem to be associated with better health-related quality of life after the Nuss procedure for PE.

Place, publisher, year, edition, pages
Oxford University Press, 2022
Keywords
Bar removal, Nuss procedure, Pectus excavatum, Quality of life
National Category
Surgery
Identifiers
urn:nbn:se:oru:diva-97474 (URN)10.1093/icvts/ivac031 (DOI)000756963000001 ()35142354 (PubMedID)2-s2.0-85134083334 (Scopus ID)
Funder
Region Örebro County, OLL-939296
Note

Funding agency:

Örebro University 2021/02220

Available from: 2022-02-14 Created: 2022-02-14 Last updated: 2023-10-17Bibliographically approved
Dreifaldt, M., Samano, N., Geijer, H., Lidén, M., Bodin, L. & de Souza, D. R. (2021). Pedicled versus skeletonized internal thoracic artery grafts: a randomized trial. Asian cardiovascular & thoracic annals, 29(6), 490-497
Open this publication in new window or tab >>Pedicled versus skeletonized internal thoracic artery grafts: a randomized trial
Show others...
2021 (English)In: Asian cardiovascular & thoracic annals, ISSN 1816-5370, Vol. 29, no 6, p. 490-497Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: Concerns have been raised regarding whether skeletonization of the internal thoracic artery could damage the graft and thereby reduces its patency. The objective of this study was to compare patency rates at mid- and long-term follow-up between pedicled and skeletonized left internal thoracic artery grafts.

METHODS: This randomized controlled trial included 109 patients undergoing coronary artery bypass surgery. The patients were assigned to receive either one pedicled or one skeletonized left internal thoracic artery graft to the left anterior descending artery. Follow-up was performed at 3 years with conventional angiography, and at 8 years with computed tomography angiography. Differences between patency rates were analyzed with Fisher's exact test and a generalized linear model.

RESULTS: The patency rates for pedicled and skeletonized left internal thoracic artery grafts were 46/48 (95.8%) versus 47/52 (90.4%), p = 0.44 at 3 years, and 40/43 (93.0%) versus 37/41 (90.2%), p = 0.71 at 8 years, respectively. The difference in patency rates for pedicled and skeletonized grafts was 5.4% (95% confidence interval: -4.2-14.5) at 3 years and 2.8% (95% confidence interval: -9.9-14.1) at 8 years. All failed grafts, except for one with a localized stenosis, were anastomosed to native coronary arteries with a stenosis less than 70%. Three patients suffered sternal wound infections (two in the pedicled group, one in the skeletonized group).

CONCLUSIONS: The skeletonization technique can be used without jeopardizing the patency of the left internal thoracic artery. The most important factor in graft failure was target artery stenosis below 70%.

Place, publisher, year, edition, pages
Medikaru Toribyun, 2021
Keywords
Coronary artery bypass, graft occlusion, saphenous vein, thoracic arteries, tissue
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:oru:diva-88165 (URN)10.1177/0218492320983491 (DOI)000669941600002 ()33334128 (PubMedID)2-s2.0-85097779720 (Scopus ID)
Note

Funding Agency:

Nyckelfonden OLL-506851

Available from: 2020-12-21 Created: 2020-12-21 Last updated: 2024-01-02Bibliographically approved
Dreifaldt, M., Mannion, J. D., Geijer, H., Lidén, M., Bodin, L. & de Souza, D. R. (2021). The no-touch saphenous vein is an excellent alternative conduit to the radial artery 8 years after coronary artery bypass grafting: A randomized trial. Journal of Thoracic and Cardiovascular Surgery, 161(2), 624-630
Open this publication in new window or tab >>The no-touch saphenous vein is an excellent alternative conduit to the radial artery 8 years after coronary artery bypass grafting: A randomized trial
Show others...
2021 (English)In: Journal of Thoracic and Cardiovascular Surgery, ISSN 0022-5223, E-ISSN 1097-685X, Vol. 161, no 2, p. 624-630Article in journal (Refereed) Published
Abstract [en]

Background: In 2004, a prospective randomized trial demonstrated that after 3 years, saphenous veins (SVs) harvested with a no touch (NT) technique had a greater patency than radial grafts for coronary bypass surgery. Here we report the 8-year follow-up data of this trial.

Methods: The trial included 108 patients undergoing coronary artery bypass grafting (CABG). Each patient was assigned to receive 1 NT SV and 1 radial artery (RA) graft to either the left or right coronary territory to complement the left internal thoracic artery (LITA). Sequential grafting was common, so overall graft patency as well as the patency of each anastomosis were assessed.

Results: Angiography was performed in 84 patients (78%) at mean of 97 months postoperatively. Graft patency were high and similar for both NT and RA: 86% for NT versus 79% for RA (P = .22). The patency of coronary anastomoses was significantly higher with the NT SV grafts (91% vs 81%; P = .046). The NT grafts also had excellent patency in coronary arteries with <90% stenosis (93% patency) and in coronary arteries of small diameter (87% patency) or with mild calcification (88% patency). Patency for the LITA was 92%.

Conclusions: NT SV grafts have excellent patency similar to that of RA grafts after 8 years. In addition, NT SV grafts can be used in situations that are not ideal for RA grafts.

Place, publisher, year, edition, pages
Elsevier, 2021
Keywords
CABG, saphenous vein, radial artery, no touch technique, patency
National Category
Cardiac and Cardiovascular Systems
Identifiers
urn:nbn:se:oru:diva-89692 (URN)10.1016/j.jtcvs.2019.09.177 (DOI)000608547600069 ()31831193 (PubMedID)2-s2.0-85075371746 (Scopus ID)
Note

Funding Agencies:

Nyckelfonden  

Regional Research Committee, University Hospital, Örebro, Sweden  

Available from: 2021-02-18 Created: 2021-02-18 Last updated: 2021-02-18Bibliographically approved
Norlander, L., Karlsson, J., Anderzen-Carlsson, A., Vidlund, M., Dreifaldt, M., Andreasson, J. & Sundqvist, A.-S. (2021). Translation and psychometric evaluation of the Swedish versions of the Nuss Questionnaire modified for Adults and the Single Step Questionnaire. Journal of Patient-Reported Outcomes, 5(1), Article ID 21.
Open this publication in new window or tab >>Translation and psychometric evaluation of the Swedish versions of the Nuss Questionnaire modified for Adults and the Single Step Questionnaire
Show others...
2021 (English)In: Journal of Patient-Reported Outcomes, E-ISSN 2509-8020, Vol. 5, no 1, article id 21Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Pectus excavatum (PE) is the most common congenital chest wall deformity. Most individuals with PE suffer from psychosocial problems, with low self-esteem and poor body image. Correctional surgery for PE is available, the most widely used is the Nuss procedure. The Nuss procedure has previously been reported to improve self-esteem, body image and health-related quality of life (HRQoL). To assess HRQoL among individuals with PE, the Nuss Questionnaire modified for Adults (NQ-mA) and Single Step Questionnaire (SSQ) has been developed. The aim of the current study was to translate and culturally adapt NQ-mA and SSQ to fit a Swedish context, and to evaluate the psychometric properties, and validate the culturally adapted versions.

METHODS: Individuals who had undergone the Nuss procedure for pectus excavatum were invited to participate in a multicentre study with cross-sectional design. HRQoL was assessed by NQ-mA, SSQ and RAND-36. Psychometric properties for NQ-mA and SSQ were evaluated by content validity index and construct validity (exploratory factor analysis, confirmatory factor analysis, and inter-scale correlations). Known-groups validity, as well as floor and ceiling effects, were evaluated. Internal consistency reliability was examined using Cronbach's alpha.

RESULTS: In total 236 individuals participated in the study. Content validity index for NQ-mA showed satisfactory results, except for two items. Factor analysis for NQ-mA suggested a two-factor model, with exclusion of two items. Subscales correlated adequately with RAND-36's domains. Known-groups validity for NQ-mA demonstrated high sensitivity for between-group differences. Ceiling effects were present in several items. Cronbach's alpha for NQ-mA was .89. Confirmatory factor analysis for SSQ resulted in a three-factor model, with exclusion of five items. However, this model was shown to be unstable through further exploratory factor analysis testing, and no further psychometric tests were conducted for SSQ.

CONCLUSION: The 10-item Swedish version of NQ-mA was shown to be valid for research and clinical assessment of HRQoL in individuals with pectus excavatum. The Swedish version of SSQ requires revision of items before further validation can be performed.

Place, publisher, year, edition, pages
Springer Nature, 2021
Keywords
Factor analysis, Nuss procedure, Pectus excavatum, Psychometrics, Quality of life
National Category
Psychology (excluding Applied Psychology)
Identifiers
urn:nbn:se:oru:diva-90006 (URN)10.1186/s41687-021-00293-2 (DOI)000705258400001 ()33629190 (PubMedID)2-s2.0-85101697856 (Scopus ID)
Funder
Region Örebro County
Note

Funding agency:

Örebro University

Available from: 2021-02-26 Created: 2021-02-26 Last updated: 2024-06-11Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-5585-1783

Search in DiVA

Show all publications