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Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Örebro University Hospital. Department of Laboratory Medicine, Section for Transfusion Medicine.
Department of Laboratory Medicine, Section for Transfusion Medicine, Örebro University Hospital.
2012 (English)In: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, no 70, UNSP e4414Article in journal (Refereed) Published
Abstract [en]

Blood centers are faced with many challenges including maximizing production yield from the blood product donations they receive as well as ensuring the highest possible level of safety for transfusion patients, including protection from transfusion transmitted diseases. This must be accomplished in a fiscally responsible manner which minimizes operating expenses including consumables, equipment, waste, and personnel costs, among others.

Several methods are available to produce platelet concentrates for transfusion. One of the most common is the buffy coat method in which a single therapeutic platelet unit (>= 2.0 x10(11) platelets per unit or per local regulations) is prepared by pooling the buffy coat layer from up to six whole blood donations. A procedure for producing "double dose" whole blood derived platelets has only recently been developed.

Presented here is a novel method for preparing double dose whole blood derived platelet concentrates from pools of 7 buffy coats and subsequently treating the double dose units with the INTERCEPT Blood System for pathogen inactivation. INTERCEPT was developed to inactivate viruses, bacteria, parasites, and contaminating donor white cells which may be present in donated blood. Pairing INTERCEPT with the double dose buffy coat method by utilizing the INTERCEPT Processing Set with Dual Storage Containers (the "DS set"), allows blood centers to treat each of their double dose units in a single pathogen inactivation processing set, thereby maximizing patient safety while minimizing costs. The double dose buffy coat method requires fewer buffy coats and reduces the use of consumables by up to 50% (e.g. pooling sets, filter sets, platelet additive solution, and sterile connection wafers) compared to preparation and treatment of single dose buffy coat platelet units. Other cost savings include less waste, less equipment maintenance, lower power requirements, reduced personnel time, and lower collection cost compared to the apheresis technique.

Place, publisher, year, edition, pages
JoVE , 2012. no 70, UNSP e4414
Keyword [en]
Medicine, Issue 70, Immunology, Hematology, Infectious Disease, Pathology, pathogen inactivation, pathogen reduction, double-dose platelets, INTERCEPT Blood System, amotosalen, UVA, platelet, blood processing, buffy coat, IBS, transfusion
National Category
Hematology Immunology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-57947DOI: 10.3791/4414ISI: 000209226100033PubMedID: 23242463Scopus ID: 2-s2.0-84874121450OAI: oai:DiVA.org:oru-57947DiVA: diva2:1106843
Note

Funding Agency:

Cerus Corp, manufacturer of the INTERCEPT Blood System

Available from: 2017-06-08 Created: 2017-06-08 Last updated: 2017-11-29Bibliographically approved

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