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The efficiency of cross-linking methods in eradication of bacteria is influenced by the riboflavin concentration and the irradiation time of ultraviolet light
Örebro University, School of Medical Sciences. Clinical Research Centre, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Medical Sciences. Department of Ophthalmology, Örebro University Hospital, Örebro, Sweden; Centre for Health Care Sciences, Örebro University Hospital, Örebro, Sweden.
Department of Ophthalmology, Ryhov County Hospital, Jönköping, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden. Örebro University Hospital. Department of Ophthalmology, Örebro University Hospital, Örebro, Sweden.
2014 (English)In: Acta Ophthalmologica, ISSN 1755-375X, E-ISSN 1755-3768, Vol. 92, no 7, p. 656-661Article in journal (Refereed) Published
Abstract [en]

Purpose: To determine bacterial eradication using numerous riboflavin concentrations and different ultraviolet light A (UVA) radiant and exposure time in an experimental model.

Methods: Dilutions of Staphylococcus epidermidis were mixed with riboflavin at varying concentrations (0.007-0.09%). Effects on bacterial growth were evaluated after 0, 3, 6, 30 and 60min of UVA exposure (irradiance 30 and 3mW/cm(2)). Standard settings of UVA were compared with high-power UVA approach. Different fluid thicknesses of the exposed dilutions were also examined to improve the model.

Results: Bacterial eradication (%) was increased after 60 compared with 30min of UVA exposure for concentrations of 0.03-0.07% but not for 0.09% riboflavin. There was a significant difference between the efficacy between 0.03 and 0.09% and eradication dropped from 80% to 50% (p=0.01). A correlation could be calculated for the amount of riboflavin at 60min of UVA and the ability to kill bacteria (p=0.01). The antibacterial effect was more pronounced when the tested bacterial suspension thickness was reduced. High-power UVA method was less potent in microbial elimination, eradicating only 60% of bacteria after 6min versus 97-99% after 60min in the low-power setting, compared with respective controls (p=0.02).

Conclusions: In these in vitro experiments, a longer UVA exposure time in combination with lower riboflavin levels were found to be favourable in killing bacteria as compared to the standard cross-linking settings. Further studies are needed to evaluate the clinical relevance of these findings.

Place, publisher, year, edition, pages
Hoboken, USA: John Wiley & Sons, 2014. Vol. 92, no 7, p. 656-661
Keywords [en]
Bacteria, cross-linking, exposure time, riboflavin, UVA
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:oru:diva-39452DOI: 10.1111/aos.12301ISI: 000344162700030PubMedID: 25493311Scopus ID: 2-s2.0-84887264034OAI: oai:DiVA.org:oru-39452DiVA, id: diva2:770391
Note

Funding Agency:

Örebro University Hospital (Sweden) - Research Funds OLL-120691

Available from: 2014-12-10 Created: 2014-12-10 Last updated: 2018-08-29Bibliographically approved

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Makdoumi, KarimCrafoord, Sven

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