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Characterization of human invasive isolates of Listeria monocytogenes in Sweden 1986-2007
Örebro universitet, Institutionen för restaurang- och måltidskunskap. Department of Clinical Medicine, Örebro University Hospital, Örebro, Sweden.
Örebro universitet, Restaurang- och hotellhögskolan - Grythytte akademi.
Örebro universitet, Restaurang- och hotellhögskolan - Grythytte akademi.ORCID-id: 0000-0003-0154-9452
Vise andre og tillknytning
2008 (engelsk)Inngår i: Foodborne pathogens and disease, ISSN 1535-3141, E-ISSN 1556-7125, Vol. 5, nr 6, s. 755-761Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Since 1986, 68% of the Listeria monocytogenes isolates from human cases of invasive listeriosis in Sweden are available for retrospective studies. The aim of the present study was to characterize 601 human invasive isolates of L. monocytogenes in Sweden from 1986 to 2007 by using serotyping and pulsed-field gel electrophoresis. Since 1996, serovar 4b was permanently reduced to the second or third most common serovar in human cases in Sweden. During the latter period, 2000-2007, only 13% belonged to serovar 4b and 71% to 1/2a. The dendrogram, based on pulsovars, reveals two clusters with different serovars. Cluster 1 exhibits serovars 4b and 1/2b, whereas cluster 2 consists of serovar 1/2a. Serovar 1/2a seems to be more heterogeneous than serovar 4b.

sted, utgiver, år, opplag, sider
Mary Ann Liebert, 2008. Vol. 5, nr 6, s. 755-761
Emneord [en]
Invasive, Listeria monocytogenes, retrospective
HSV kategori
Forskningsprogram
Mikrobiologi; Måltidskunskap
Identifikatorer
URN: urn:nbn:se:oru:diva-2997DOI: 10.1089/fpd.2008.0123ISI: 000262028400007PubMedID: 18847381Scopus ID: 2-s2.0-57749091480OAI: oai:DiVA.org:oru-2997DiVA, id: diva2:136258
Merknad

The work is done in cooperation with the School of Hospitality, Culinary Arts & Meal Science, Örebro University

Tilgjengelig fra: 2008-09-05 Laget: 2008-09-05 Sist oppdatert: 2018-01-13bibliografisk kontrollert
Inngår i avhandling
1. Human listeriosis: sources and routes
Åpne denne publikasjonen i ny fane eller vindu >>Human listeriosis: sources and routes
2008 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

The bacterium Listeria monocytogenes can cause the disease listeriosis in both humans and animals. For the epidemiological investigation of listeriosis detection and characterisation of the organism are important steps.

Paper I. There are few reports on the incidence of L. monocytogenes in clinical samples from humans in India. Therefore, we investigated 144 samples from immunocompromised patients. L. monocytogenes was isolated from two placental bits from women with poor obstetric history, one patient with renal failure and three other patients. Five isolates were positive for the virulence genes hlyA, actA and iap. The sixth isolate was positive for hlyA and actA genes.

Paper II. Characterisation of 601 human L. monocytogenes isolates causing invasive listeriosis during the period 1986 to 2007 in Sweden reveals a decrease in serovar 4b strains. Since 1996, serovar 1/2a has become the predominant serovar causing human listeriosis: PFGE analysis revealed two clusters including different serotypes suggesting that we need more studies on genetic relatedness among clinical isolates.

Paper III. The incidence of Listeria species in seafood from markets in Goa was studied. One hundred and fifteen raw/fresh seafoods bought at the fish markets were sampled and tested for presence of Listeria spp. L. monocytogenes was detected in 10 samples. L. monocytogenes in raw seafood may pose a health risk in kitchen if contaminating ready-to-eat food.

Paper IV. Gravad and cold-smoked salmon are associated with human listeriosis in Sweden. L. monocytogenes was isolated from 11 of 56 products. Serovar 1/2a was predominant, followed by 4b. REA/PFGE typing of the isolates identified five types of L. monocytogenes. One type was identical to a human type, two other were closely related.These findings suggest that gravad and cold-smoked salmon are still possible sources of listeriosis in Sweden.

Paper V. Many outbreaks of listeriosis have been related to consumption of dairy-associated products. Therefore, 123 farm bulk milk samples in India and 20 cervico-vaginal samples from dairy cows with reproductive disorders were investigated for L. monocytogenes.

L. monocytogenes was isolated from 17.9% of bulk milk samples and from 10% of cervico-vaginal swabs. The virulence gene hlyA was detected in all isolates. These findings represent a public health risk where unpasteurised milk and milk products are largely consumed.

Paper VI. Isolates of L. monocytogenes (n=177) from 22 animal species were characterized and compared with human strains isolated between 1986-2006 in Sweden. Although many animals and humans shared pulsovars, they did not appear at the same time or with the same proportion of strains. The pulsovars shared by both animals and humans may indicate that there is an exchange of L. monocytogenes strains between these two groups due to either direct or indirect transmission.

 

sted, utgiver, år, opplag, sider
Örebro: Örebro universitet, 2008. s. 89
Serie
Örebro Studies in Medicine, ISSN 1652-4063 ; 19
Emneord
Listeria monocytogenes, listeriosis, zoonoses, PFGE, food safety, incidence
HSV kategori
Forskningsprogram
Biomedicin
Identifikatorer
urn:nbn:se:oru:diva-2447 (URN)978-91-7668-612-6 (ISBN)
Disputas
2008-09-29, Gastronomiska teatern, Måltidens hus, Sörälgsvägen 2, Grythyttan, 13:00 (engelsk)
Opponent
Veileder
Merknad

The work is done in cooperation with the School of Hospitality, Culinary Arts & Meal Science, Örebro UniversityVishal Singh Parihar, Örebro University, Department of Restaurant and Culinary Arts, Sörälgsvägen 2, SE-712 60 Grythyttan, Sweden or ICAR Research Complex for Goa, Ela, Old Goa – 403402, Goa, India. Phone 0832-2284678/79; Fa:0832-2285649. E-mail: drvishu@yahoo.co.in

Tilgjengelig fra: 2008-09-05 Laget: 2008-09-05 Sist oppdatert: 2017-10-18bibliografisk kontrollert

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