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Scanning electrochemical microscopy of single human urinary bladder cells using reactive oxygen species as probe of inflammatory response
Dept Chem, Univ Western Ontario, London ON, Canada.
Univ Western Ontario, London ON, Canada.
Örebro universitet, Akademin för naturvetenskap och teknik. Univ Western Ontario, London ON, Canada.ORCID-id: 0000-0001-7957-0310
Dept Chem, Univ Western Ontario, London ON, Canada.
2010 (engelsk)Inngår i: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 12, nr 6, s. 773-776Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Scanning electrochemical microscopy of single human bladder (T24) and kidney (A498) epithelial cells after stimulation with heat-killed uropathogenic Escherichia coli GR-12 was investigated for the first time. 124 cells treated with the hk-UPEC in time-lapsed images showed prolonged release of reactive oxygen species (ROS) due to toll-like receptors (TLR4), while A498 cells without TLR4 did not show the ROS increase after the dosage of the same stimulant. This indicates discrete pathways of triggering the inflammatory. It also reveals that ROS can be used as a probe to detect inflammatory response to gram-negative bacteria. (C) 2010 Elsevier B.V. All rights reserved.

sted, utgiver, år, opplag, sider
2010. Vol. 12, nr 6, s. 773-776
Emneord [en]
Scanning electrochemical microscopy, Reactive oxygen species, Ultramicroelectrode, Inflammatory response, Live cells, Toll-like receptors
HSV kategori
Forskningsprogram
Biologi
Identifikatorer
URN: urn:nbn:se:oru:diva-12931DOI: 10.1016/j.elecom.2010.03.030ISI: 000279215700016OAI: oai:DiVA.org:oru-12931DiVA, id: diva2:383269
Tilgjengelig fra: 2011-01-04 Laget: 2011-01-03 Sist oppdatert: 2018-04-23bibliografisk kontrollert

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