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Increased number of double positive CD3+ CD8+ CD4+ lamina propria T lymphocyte in gut mucosa of post infectious IBS patients compared to healthy controls
Örebro universitet, Institutionen för hälsovetenskap och medicin.ORCID-id: 0000-0003-4713-1772
Örebro universitet, Institutionen för läkarutbildning.ORCID-id: 0000-0002-0362-0008
Örebro universitet, Institutionen för läkarutbildning.ORCID-id: 0000-0001-5460-8888
Örebro universitet, Institutionen för hälsovetenskap och medicin.ORCID-id: 0000-0003-3383-9219
2012 (engelsk)Inngår i: Neurogastroenterology and Motility, ISSN 1350-1925, E-ISSN 1365-2982, Vol. 24, nr Suppl. 2, s. 104-105Artikkel i tidsskrift, Meeting abstract (Annet vitenskapelig) Published
Abstract [en]

Objective: Irritable bowel syndrome (IBS) developed after a gastroenteritis is denoted post infectious IBS (PI-IBS) and is thought to represent a specific patho-physiological entity. Naive CD8+CD45RA+cytotoxic T lymphocytes recognize antigen derived from intracellular bacteria and viruses. Naive CD4+CD45RA+helper T lymphocytes are activated by the antigen from extracellular microorganisms with the help of antigen-presenting cells. Activated CD4+CD45RO+helper T lymphocytes help phagocytes to kill microbes and activate naive B lymphocytes.

Aim: To characterize subsets of mucosal lymphocytes in PI-IBS with flow cytometry analysis as a lead in identifying new therapeutic methods.

Methods: 5 PI-IBS patients and 6 healthy individuals were recruited. We performed a distal colonoscopy without bowel cleansing or other preparation. Lamina propria lymphocytes (LPL) and intra-epithelial lymphocytes (IEL) were isolated from sigmodal biopsies and sub classified by CD3, CD4, CD8, CD45RO and CD45RA with flow cytometry.

Results: We observed a significant deference (P < 0.01, two-tailed Mann–Whitney test) in double positive CD3+CD8+CD4+LPL between PI-IBS patient and healthy controls. We also observed a trend towards increased frequency of CD3+CD4+LPL in PI-IBS patients. Additionally, PI-IBS patients showed an increased frequency of CD3+CD8+LPL and IEL. The proportion of memory / activated CD45RO+CD4+LPL was higher in PI-IBS patients, and its proportion of the CD8+population was lower compared with the healthy controls. On the contrary the proportion of naive CD45RA+CD4+LPL was lower in PI-IBS patients compared with healthy controls. However, we found no differences in the distribution naı¨ve / activated CD4+ and CD8+IEL between PI-IBS patients and healthy controls.

Conclusion: The difference in double positive (DP) CD3+CD8+CD4+LPL seen between PI-IBS patient and healthy controls is consistent with findings of increased number DP T cells in target organs in immuno-inflammatory conditions. Our analysis revealed that the normal gut has a greater variation in the prevalence of CD3+CD4+and CD4+CD45RO+LPL than that of PI-IBS patients. These findings confirm aberrant mucosal subsets of lymphocytes. However, more subjects need to be included in the study in order to draw firm conclusions.

sted, utgiver, år, opplag, sider
Wiley-Blackwell, 2012. Vol. 24, nr Suppl. 2, s. 104-105
HSV kategori
Forskningsprogram
Neurologi
Identifikatorer
URN: urn:nbn:se:oru:diva-24425ISI: 000308435400338OAI: oai:DiVA.org:oru-24425DiVA, id: diva2:544563
Konferanse
The European Society for Clinical Nutrition and Metabolism, Barcelona, Spain, September 8-11, 2012
Merknad

Även NGM - Joint International Neurogastroenterology and Motility Meeting, September 6-8, 2012, Bologna, Italy

Tilgjengelig fra: 2012-08-15 Laget: 2012-08-15 Sist oppdatert: 2017-12-07bibliografisk kontrollert

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Sundin, JohannaBrummer, RobertHultgren Hörnquist, Elisabet

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