oru.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
Örebro universitet, Institutionen för naturvetenskap och teknik.ORCID-id: 0000-0002-8162-8945
2016 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

sted, utgiver, år, opplag, sider
Örebro: Örebro university , 2016. , s. 80
Serie
Örebro Studies in Life Science ; 15
Emneord [en]
Heavy Metals, Pollution, Accumulation, Remediation, Human Health, Bacteria, Genome Sequencing, de novo Assembly, Gene Prediction
HSV kategori
Forskningsprogram
Biologi
Identifikatorer
URN: urn:nbn:se:oru:diva-51436ISBN: 978-91-7529-146-8 (tryckt)OAI: oai:DiVA.org:oru-51436DiVA, id: diva2:949852
Disputas
2016-09-22, Högskolan i Skövde, G-building, lecture hall G111, Högskolevägen 28, Skövde, 13:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2016-07-25 Laget: 2016-07-25 Sist oppdatert: 2018-12-07bibliografisk kontrollert
Delarbeid
1. Isolation and characterization of a Lysinibacillus strain B1-CDA showing potential for bioremediation of arsenics from contaminated water
Åpne denne publikasjonen i ny fane eller vindu >>Isolation and characterization of a Lysinibacillus strain B1-CDA showing potential for bioremediation of arsenics from contaminated water
Vise andre…
2014 (engelsk)Inngår i: Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, ISSN 1093-4529, E-ISSN 1532-4117, Vol. 49, nr 12, s. 1349-1360Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The main objective of this study was to identify and isolate arsenic resistant bacteria that can be used for removing arsenic from the contaminated environment. Here we report a soil borne bacterium, B1-CDA that can serve this purpose. B1-CDA was isolated from the soil of a cultivated land in Chuadanga district located in the southwest region of Bangladesh. The morphological, biochemical and 16S rRNA analysis suggested that the isolate belongs to Lysinibacillus sphaericus. The minimum inhibitory concentration (MIC) value of the isolate is 500mM (As) as arsenate. TOF-SIMS and ICP-MS analysis confirmed intracellular accumulation and removal of arsenics. Arsenic accumulation in cells amounted to 5.0mg g(-1) of the cells dry biomass and thus reduced the arsenic concentration in the contaminated liquid medium by as much as 50%. These results indicate that B1-CDA has the potential for remediation of arsenic from the contaminated water. We believe the benefits of implementing this bacterium to efficiently reduce arsenic exposure will not only help to remove one aspect of human arsenic poisoning but will also benefit livestock and native animal species. Therefore, the outcome of this research will be highly significant for people in the affected area and also for human populations in other countries that have credible health concerns as a consequence of arsenic-contaminated water.

Emneord
Pollution, toxic metals, arsenics, bioremediation, bacteria, bioaccumulation
HSV kategori
Forskningsprogram
Miljövetenskap
Identifikatorer
urn:nbn:se:oru:diva-36537 (URN)10.1080/10934529.2014.928247 (DOI)000340370000002 ()25072766 (PubMedID)2-s2.0-84905275614 (Scopus ID)
Forskningsfinansiär
Sida - Swedish International Development Cooperation AgencySwedish Research Council Formas
Merknad

Funding Agency:

Nilsson-Ehle (The Royal Physiographic Society in Lund) foundation in Sweden

Tilgjengelig fra: 2014-09-16 Laget: 2014-09-15 Sist oppdatert: 2018-12-07bibliografisk kontrollert
2. Bioremediation of hexavalent chromium (VI) by a soil-borne bacterium, Enterobacter cloacae B2-DHA
Åpne denne publikasjonen i ny fane eller vindu >>Bioremediation of hexavalent chromium (VI) by a soil-borne bacterium, Enterobacter cloacae B2-DHA
Vise andre…
2015 (engelsk)Inngår i: Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering, ISSN 1093-4529, E-ISSN 1532-4117, Vol. 50, nr 11, s. 1136-1147Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Chromium and chromium containing compounds are discharged into the nature as waste from anthropogenic activities, such as industries, agriculture, forest farming, mining and metallurgy. Continued disposal of these compounds to the environment leads to development of various lethal diseases in both humans and animals. In this paper, we report a soil borne bacterium, B2-DHA that can be used as a vehicle to effectively remove chromium from the contaminated sources. B2-DHA is resistant to chromium with a MIC value of 1000 mu g mL(-1) potassium chromate. The bacterium has been identified as a Gram negative, Enterobacter cloacae based on biochemical characteristics and 16S rRNA gene analysis. TOF-SIMS and ICP-MS analyses confirmed intracellular accumulation of chromium and thus its removal from the contaminated liquid medium. Chromium accumulation in cells was 320 mu g/g of cells dry biomass after 120-h exposure, and thus it reduced the chromium concentration in the liquid medium by as much as 81%. Environmental scanning electron micrograph revealed the effect of metals on cellular morphology of the isolates. Altogether, our results indicate that B2-DHA has the potential to reduce chromium significantly to safe levels from the contaminated environments and suggest the potential use of this bacterium in reducing human exposure to chromium, hence avoiding poisoning.

Emneord
Bioremediation, chromium, Enterobacter cloacae, human health, soil borne bacterium, tannery effluents
HSV kategori
Forskningsprogram
Miljövetenskap
Identifikatorer
urn:nbn:se:oru:diva-45753 (URN)10.1080/10934529.2015.1047670 (DOI)000359339900006 ()26191988 (PubMedID)2-s2.0-84937800926 (Scopus ID)
Forskningsfinansiär
Sida - Swedish International Development Cooperation Agency, AKT-2010-018Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, 229-2007-217
Merknad

Funding Agency:

Nilsson-Ehle (The Royal Physiographic Society in Lund) Foundation in Sweden

Tilgjengelig fra: 2015-09-09 Laget: 2015-09-09 Sist oppdatert: 2018-12-07bibliografisk kontrollert
3. Biosorption of nickel by Lysinibacillus sp BA2 native to bauxite mine
Åpne denne publikasjonen i ny fane eller vindu >>Biosorption of nickel by Lysinibacillus sp BA2 native to bauxite mine
Vise andre…
2014 (engelsk)Inngår i: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 107, s. 260-268Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The current scenario of environmental pollution urges the need for an effective solution for toxic heavy metal removal from industrial wastewater. Bioremediation is the most cost effective process employed by the use of microbes especially bacteria resistant to toxic metals. In this study, Lysinibacillus sp. BA2, a nickel tolerant strain isolated from bauxite mine was used for the biosorption of Ni(II). Lysinibacillus sp. BA2 biomass had isoelectric point (pI) of 3.3. The maximum negative zeta potential value (-39.45) was obtained at pH 6.0 which was highly favourable for Ni(II) biosorption. 238.04 mg of Ni(II) adsorbed on one gram of dead biomass and 196.32 mg adsorbed on one gram of live biomass. The adsorption of Ni(II) on biomass increased with time and attained saturation after 180 mm with rapid biosorption in initial 30 min. The Langmuir and Freundlich isotherms could fit well for biosorption of Ni(II) by dead biomass while Langmuir isotherm provided a better fit for live biomass based on correlation coefficient values. The kinetic studies of Ni(II) removal, using dead and live biomass was well explained by second-order kinetic model. Ni(II) adsorption on live biomass was confirrned by SEM-EDX where cell aggregation and increasing irregularity of cell morphology was observed even though cells were in non-growing state. The FTIR analysis of biomass revealed the presence of carboxyl, hydroxyl and amino groups, which seem responsible for biosorption of Ni(II). The beads made using dead biomass of Lysinibacillus sp. BA2 could efficiently remove Ni(II) from effluent solutions. These microbial cells can substitute expensive methods for treating nickel contaminated industrial wastewaters.

sted, utgiver, år, opplag, sider
Elsevier, 2014
Emneord
Lysinibacillus sp BA2, Heavy metals, Biosorption, Adsorption isotherm
HSV kategori
Forskningsprogram
Biologi
Identifikatorer
urn:nbn:se:oru:diva-51857 (URN)10.1016/j.ecoenv.2014.06.009 (DOI)000342122000036 ()25011123 (PubMedID)2-s2.0-84903900011 (Scopus ID)
Tilgjengelig fra: 2016-08-29 Laget: 2016-08-29 Sist oppdatert: 2018-12-07bibliografisk kontrollert
4. Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
Åpne denne publikasjonen i ny fane eller vindu >>Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
Vise andre…
2015 (engelsk)Inngår i: Genomics, ISSN 0888-7543, E-ISSN 1089-8646, Vol. 106, nr 6, s. 384-392Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Previously, we reported an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, isolated from an arsenic contaminated lands. Here, we have investigated its genetic composition and evolutionary history by using massively parallel sequencing and comparative analysis with other known Lysinibacillus genomes. Assembly of the sequencing reads revealed a genome of similar to 4.5 Mb in size encompassing similar to 80% of the chromosomal DNA. We found that the set of ordered contigs contains abundant regions of similarity with other Lysinibacillus genomes and clearly identifiable genome rearrangements. Furthermore, all genes of B1-CDA that were predicted be involved in its resistance to arsenic and/or other heavy metals were annotated. The presence of arsenic responsive genes was verified by PCR in vitro conditions. The findings of this study highlight the significance of this bacterium in removing arsenics and other toxic metals from the contaminated sources. The genetic mechanisms of the isolate could be used to cope with arsenic toxicity.

sted, utgiver, år, opplag, sider
Academic Press, 2015
Emneord
Toxic metals, Bioremediation, Lysinibacillus sphaericus B1-CDA, Genome sequencing, de novo assembly, Gene prediction
HSV kategori
Forskningsprogram
Miljövetenskap
Identifikatorer
urn:nbn:se:oru:diva-47292 (URN)10.1016/j.ygeno.2015.09.006 (DOI)000365613100010 ()26387925 (PubMedID)2-s2.0-84948102629 (Scopus ID)
Merknad

Funding Agencies:

Swedish International Development Cooperation Agency (SIDA) AKT-2010-018

Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS) 229-2007-217

Nilsson-Ehle (The Royal Physio-graphic Society in Lund) foundation in Sweden

Tilgjengelig fra: 2016-01-05 Laget: 2016-01-04 Sist oppdatert: 2018-12-07bibliografisk kontrollert
5. Genome analysis of Enterobacter cloacae B2-DHA: A bacterium resistant to chromium and/or other heavy metals
Åpne denne publikasjonen i ny fane eller vindu >>Genome analysis of Enterobacter cloacae B2-DHA: A bacterium resistant to chromium and/or other heavy metals
Vise andre…
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Forskningsprogram
Biologi
Identifikatorer
urn:nbn:se:oru:diva-51858 (URN)
Tilgjengelig fra: 2016-08-31 Laget: 2016-08-29 Sist oppdatert: 2018-12-06bibliografisk kontrollert

Open Access i DiVA

Cover(605 kB)63 nedlastinger
Filinformasjon
Fil COVER01.pdfFilstørrelse 605 kBChecksum SHA-512
86d5a9fe27507475b59c531a51951df2250e4ebe7f3e06b1bc13efe16e4e408fc7fc4c6e2782ba315f7f5cf611e24b4ad137310a980bec421389c75e5c30fc7a
Type coverMimetype application/pdf
Spikblad(106 kB)64 nedlastinger
Filinformasjon
Fil SPIKBLAD01.pdfFilstørrelse 106 kBChecksum SHA-512
b223d269bb901796d3f18b0ef13177af4348e78596157eaf4a882c22b4160171b1191a84cdd19c07a0cdcc5cd377d09a1fc501af443cb84e719c9cd177c55cc3
Type spikbladMimetype application/pdf
Introductory chapter(1883 kB)843 nedlastinger
Filinformasjon
Fil FULLTEXT01.pdfFilstørrelse 1883 kBChecksum SHA-512
5ffbdc5ede62ca20938c9e325cc5d7406599fb87c436121784aac548afc7bb6c3cea784dfcfaa5bff157f2bf4b825f66594ce15088dc838cd1fc7df3aca7b391
Type fulltextMimetype application/pdf

Personposter BETA

Rahman, Aminur

Søk i DiVA

Av forfatter/redaktør
Rahman, Aminur
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar
Totalt: 843 nedlastinger
Antall nedlastinger er summen av alle nedlastinger av alle fulltekster. Det kan for eksempel være tidligere versjoner som er ikke lenger tilgjengelige

isbn
urn-nbn

Altmetric

isbn
urn-nbn
Totalt: 1648 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf