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Biosorption of nickel by Lysinibacillus sp BA2 native to bauxite mine
Dr DY Patil Biotechnol & Bioinformat Inst, Dr DY Patil Vidyapeeth, Pune, India.
Dr DY Patil Biotechnol & Bioinformat Inst, Dr DY Patil Vidyapeeth, Pune, India.
Dr DY Patil Biotechnol & Bioinformat Inst, Dr DY Patil Vidyapeeth, Pune, India.
University of Skövde, Skövde, Sweden.
Vise andre og tillknytning
2014 (engelsk)Inngår i: Ecotoxicology and Environmental Safety, ISSN 0147-6513, E-ISSN 1090-2414, Vol. 107, s. 260-268Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The current scenario of environmental pollution urges the need for an effective solution for toxic heavy metal removal from industrial wastewater. Bioremediation is the most cost effective process employed by the use of microbes especially bacteria resistant to toxic metals. In this study, Lysinibacillus sp. BA2, a nickel tolerant strain isolated from bauxite mine was used for the biosorption of Ni(II). Lysinibacillus sp. BA2 biomass had isoelectric point (pI) of 3.3. The maximum negative zeta potential value (-39.45) was obtained at pH 6.0 which was highly favourable for Ni(II) biosorption. 238.04 mg of Ni(II) adsorbed on one gram of dead biomass and 196.32 mg adsorbed on one gram of live biomass. The adsorption of Ni(II) on biomass increased with time and attained saturation after 180 mm with rapid biosorption in initial 30 min. The Langmuir and Freundlich isotherms could fit well for biosorption of Ni(II) by dead biomass while Langmuir isotherm provided a better fit for live biomass based on correlation coefficient values. The kinetic studies of Ni(II) removal, using dead and live biomass was well explained by second-order kinetic model. Ni(II) adsorption on live biomass was confirrned by SEM-EDX where cell aggregation and increasing irregularity of cell morphology was observed even though cells were in non-growing state. The FTIR analysis of biomass revealed the presence of carboxyl, hydroxyl and amino groups, which seem responsible for biosorption of Ni(II). The beads made using dead biomass of Lysinibacillus sp. BA2 could efficiently remove Ni(II) from effluent solutions. These microbial cells can substitute expensive methods for treating nickel contaminated industrial wastewaters.

sted, utgiver, år, opplag, sider
Elsevier, 2014. Vol. 107, s. 260-268
Emneord [en]
Lysinibacillus sp BA2, Heavy metals, Biosorption, Adsorption isotherm
HSV kategori
Forskningsprogram
Biologi
Identifikatorer
URN: urn:nbn:se:oru:diva-51857DOI: 10.1016/j.ecoenv.2014.06.009ISI: 000342122000036PubMedID: 25011123Scopus ID: 2-s2.0-84903900011OAI: oai:DiVA.org:oru-51857DiVA, id: diva2:956185
Tilgjengelig fra: 2016-08-29 Laget: 2016-08-29 Sist oppdatert: 2018-12-07bibliografisk kontrollert
Inngår i avhandling
1. Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
Åpne denne publikasjonen i ny fane eller vindu >>Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
2016 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

sted, utgiver, år, opplag, sider
Örebro: Örebro university, 2016. s. 80
Serie
Örebro Studies in Life Science ; 15
Emneord
Heavy Metals, Pollution, Accumulation, Remediation, Human Health, Bacteria, Genome Sequencing, de novo Assembly, Gene Prediction
HSV kategori
Forskningsprogram
Biologi
Identifikatorer
urn:nbn:se:oru:diva-51436 (URN)978-91-7529-146-8 (ISBN)
Disputas
2016-09-22, Högskolan i Skövde, G-building, lecture hall G111, Högskolevägen 28, Skövde, 13:15 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2016-07-25 Laget: 2016-07-25 Sist oppdatert: 2018-12-07bibliografisk kontrollert

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