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Antibacterial effects of lactobacillus and bacteriocin NC8 αβ on the periodontal pathogen Porphyromonas gingivalis
Örebro universitet, Institutionen för hälsovetenskap och medicin.ORCID-id: 0000-0002-3373-7864
Örebro universitet, Institutionen för hälsovetenskap och medicin. PEAS Institut AB, Söderleden 1, Linköping.
Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
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(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Nationell ämneskategori
Mikrobiologi inom det medicinska området
Forskningsämne
Biomedicin
Identifikatorer
URN: urn:nbn:se:oru:diva-46730OAI: oai:DiVA.org:oru-46730DiVA, id: diva2:873472
Tillgänglig från: 2015-11-24 Skapad: 2015-11-24 Senast uppdaterad: 2024-03-21Bibliografiskt granskad
Ingår i avhandling
1. Development of novel tools for prevention and diagnosis of Porphyromonas gingivalis infection and periodontitis
Öppna denna publikation i ny flik eller fönster >>Development of novel tools for prevention and diagnosis of Porphyromonas gingivalis infection and periodontitis
2015 (Engelska)Licentiatavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Periodontitis is a chronic infectious disease causing inflammation and destruction of tooth-supporting structures eventually leading to tooth loss. Periodontitis is induced by different subgingival microorganisms in combination with an excessive and dysregulated immune-inflammatory response against this microbial challenge. The pathogenicity of the periodontal biofilm is highly dependent upon some "keystone" species of which Porphyromonas gingivalis is considered to be one of the most important pathogens. P. gingivalis expresses a broad range of virulence factors including lipopolysaccharides (LPS), fimbriae, adhesins, hemagglutinins and an array of proteolytic enzymes. Among these factors, cysteine proteases (gingipains) are of special importance both for the bacterial survival/proliferation and for the pathological outcome. Gingipains consist of arginine-specific proteases (RgpA and RgpB) and lysine-specific protease (Kgp). The major aim of this thesis was to develop and test novel methods for diagnosis and prevention of P. gingivalis infection and periodontitis. In study I, host lymphocytes were stimulated with P. gingivalis to develop antibodies in vitro and the anti-P. gingivalis antibodies were used for immunodetection in clinical samples. The in vitro method of antibody production developed during this study could be used for an efficient real-time detection of P. gingivalis infection and periodontitis, while the attenuating effects of the antibodies suggest a role in passive immunization to prevent periodontitis and its associated diseases. In study II, we have elucidated the properties and antimicrobial effects of different lactobacillus species and the two-peptide bacteriocin NC8 αβ on P. gingivalis. NC8 αβ was found to be efficient against P. gingivalis through bacterial binding followed by permeabilization of the membranes. Liposomal systems were acquired to verify membrane permeabilization by NC8 αβ. The antimicrobial activity of NC8 αβ was found to be rapid, potent and instant. In conclusion, soluble or immobilized NC8 αβ bacteriocins may be used to prevent P. gingivalis colonization and subsequent pathogenicity, and thus supplement the host immune system against invading pathogens associated with periodontitis.

Ort, förlag, år, upplaga, sidor
Örebro: Örebro University, 2015. s. 30
Nationell ämneskategori
Mikrobiologi inom det medicinska området
Forskningsämne
Biomedicin
Identifikatorer
urn:nbn:se:oru:diva-46712 (URN)
Tillgänglig från: 2015-11-24 Skapad: 2015-11-23 Senast uppdaterad: 2018-01-10Bibliografiskt granskad
2. Novel methods for diagnosis and treatment of periodontal disease
Öppna denna publikation i ny flik eller fönster >>Novel methods for diagnosis and treatment of periodontal disease
2024 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Periodontitis is an inflammatory condition affecting tooth-supportive structures, the periodontium. Gingival tissues respond to biofilm formation by initiating an inflammatory process. If left untreated, inflammation progresses to a non-reversible condition in susceptible individuals, causing degradation of the underlying bone structures, where teeth become mobile and tooth-loss eventually occurs. Periodontitis is very common, affecting about half of the adult population. The disease is not only a concern for oral health but is linked to many systemic conditions, such as cardiovascular disease, diabetes, rheumatoid arthritis, and Alzheimer’s.

The studies included in this thesis aim to develop new strategies for the diagnosis and treatment of periodontal disease. Current diagnostic practices rely on probing and x-ray examination. There is a need for more sophisticated diagnostic tools. We have developed different sensing strategies for detection of a key-pathogen in periodontitis, Porphyromonas gingivalis, and its secreted enzymes, gingipains. The proteolytic activity of this pathogen, which drives the destructive effects of the disease, can be detected at low levels by utilizing gold nanoparticles and nanoplasmonic sensing in different ways.

Treatment of periodontitis is basically limited to the removal of soft and hard biofilms. This requires significant resources, from patients as well as clinicians, and maintenance phase is often life-long. We have identified and tested the antibacterial effect of an antimicrobial peptide derived from Lactobacillus plantarum. This bacteriocin, plantaricin NC8αβ, has been shown to effectively inhibit P. gingivalis. The bacteriocin has been further optimized into a short peptide sequence, C5, which can rupture outer membrane vesicles from P. gingivalis. Taken together, these results suggest new sensing strategies for novel diagnostic tools for periodontal disease and define a promising antibacterial agent for periodontal treatment in the future.

Ort, förlag, år, upplaga, sidor
Örebro: Örebro University, 2024. s. 58
Serie
Örebro Studies in Medicine, ISSN 1652-4063 ; 289
Nyckelord
Periodontitis, inflammation, Porphyromonas gingivalis, gingipains, biosensing, gold nanoparticles, antimicrobial treatment, NC8
Nationell ämneskategori
Annan medicinsk grundvetenskap
Identifikatorer
urn:nbn:se:oru:diva-111438 (URN)9789175295510 (ISBN)
Disputation
2024-04-12, Örebro universitet, Campus USÖ, hörsal X3, Södra Grev Rosengatan 32, Örebro, 09:00 (Svenska)
Opponent
Handledare
Tillgänglig från: 2024-02-07 Skapad: 2024-02-07 Senast uppdaterad: 2024-03-25Bibliografiskt granskad

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Khalaf, HazemNakka, Sravya SowdaminiScherbak, NikolaiBengtsson, Torbjörn

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