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Lineage II (Serovar 1/2a and 1/2c) Human Listeria monocytogenes Pulsed-Field Gel Electrophoresis Types Divided into PFGE Groups Using the Band Patterns Below 145.5 kb
Örebro universitet, Restaurang- och hotellhögskolan.
Örebro universitet, Restaurang- och hotellhögskolan.ORCID-id: 0000-0003-0154-9452
Department of Medical Microbiology and Immunology, Creighton University Medical Center, School of Medicine, Omaha NE, USA.
Örebro universitet, Restaurang- och hotellhögskolan.ORCID-id: 0000-0001-6903-5322
2017 (Engelska)Ingår i: Foodborne pathogens and disease, ISSN 1535-3141, E-ISSN 1556-7125, Vol. 14, nr 1, s. 8-16Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Among 504 clinical lineage II isolates of Listeria monocytogenes isolated during 1958-2010 in Sweden, 119 pulsed-field gel electrophoresis (PFGE) types (AscI) have been identified based on the number and distribution of all banding patterns in each DNA profile. In this study, these types were further divided into PFGE groups based on the configuration of small bands with sizes <145.5 kb. The 504 isolates included 483 serovar 1/2a isolates distributed into 114 PFGE types and 21 serovar 1/2c isolates distributed into 9 PFGE types; these were further divided into 21 PFGE groups. PFGE group, that is, configuration of small bands below 145.5 kb, and serovars were correlated. L. monocytogenes isolates belonging to PFGE groups A, B, C, E, F, H, K, L, M, S, V, W, Y, and Ö-6 to Ö-12 shared serovar 1/2a, with one exception. PFGE group E also included two PFGE types sharing serovar 1/2c and four PFGE types belonging to either serovar 1/2a or 1/2c. Isolates belonging to PFGE group N shared serovar 1/2c. In contrast to lineage I isolates, small fragments <33.3 kb were visible in all L. monocytogenes isolates belonging to lineage II. In the results from both the present and previous studies, the genomic region of small bands was genetically more conservative than in large bands. The distribution of these small bands established the relatedness of strains and defined a genetic marker for both lineages I and II, while also establishing their serogroup. The division of L. monocytogenes PFGE types into PFGE groups is advantageous as the profile of every new isolate can be identified easily and quickly through first studying the PFGE group affiliation of the isolate based on the smaller band patterns <145.5 kb, and then identifying the PFGE type based on the band patterns >145.5 kb.

Ort, förlag, år, upplaga, sidor
New Rochelle, USA: Mary Ann Liebert, 2017. Vol. 14, nr 1, s. 8-16
Nyckelord [en]
PFGE type, PFGE group, Listeria monocytogenes, serovar, lineage II
Nationell ämneskategori
Genetik Livsmedelsvetenskap
Identifikatorer
URN: urn:nbn:se:oru:diva-53597DOI: 10.1089/fpd.2016.2173ISI: 000391818800002PubMedID: 27860487Scopus ID: 2-s2.0-85009260194OAI: oai:DiVA.org:oru-53597DiVA, id: diva2:1048631
Anmärkning

Funding Agency:

Stiftelsen Grythytte Akademi Stipendiefond

Tillgänglig från: 2016-11-21 Skapad: 2016-11-21 Senast uppdaterad: 2018-07-28Bibliografiskt granskad

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Lopez-Valladares, GloriaDanielsson-Tham, Marie-LouiseTham, Wilhelm

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