oru.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Development of Escherichia coli-based gene expression profiling of sewage sludge leachates
School of Science and Technology, Örebro University, Örebro, Sweden; Molecular Biology Division Bhabha Atomic Research Centre, Mumbai, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai, India. (The Life Science Center-Biology)
Örebro universitet, Institutionen för naturvetenskap och teknik. (The Life Science Center-Biology)
Eskilstuna Strängnäs Energi & Miljö AB, Eskilstuna, Sweden.
Örebro universitet, Institutionen för naturvetenskap och teknik. (The Life Science Center-Biology)ORCID-id: 0000-0001-7336-6335
Visa övriga samt affilieringar
2018 (Engelska)Ingår i: Journal of Applied Microbiology, ISSN 1364-5072, E-ISSN 1365-2672, Vol. 125, nr 5, s. 1502-1517Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

AIMS: The impact of municipal waste on pathogenic microorganisms released into the environment is a public health concern. The present study aims to evaluate the effects of sewage sludge and antibiotic contaminants on stress response, virulence and antibiotic resistance in a pathogenic Escherichia coli.

METHODS AND RESULTS: The effects of sewage sludge leachates on uropathogenic E. coli CFT073 were determined by monitoring the expression of 45 genes associated with antibiotic/metal resistance, stress response and virulence using RT-qPCR. The E. coli gene expression was validated using sub-inhibitory concentrations of tetracycline and ciprofloxacin. E. coli exposed to sewage sludge or sewage sludge-fly ash leachates altered the expression of 5 antibiotic and metal resistance, 3 stress response and 2 virulence associated genes. When antibiotics were combined with sludge or sludge-fly ash the antibiotic-associated gene expression was altered.

CONCLUSIONS: E. coli treated with two sludge leachates had distinct gene expression patterns that were altered when the sludge leachates were combined with tetracycline, although to a lesser extent with ciprofloxacin.

SIGNIFICANCE AND IMPACT OF STUDY: The E. coli multigene expression analysis is a potential new tool for assessing the effects of pollutants on pathogenic microbes in environmental waters for improved risk assessment.

Ort, förlag, år, upplaga, sidor
Blackwell Publishing, 2018. Vol. 125, nr 5, s. 1502-1517
Nyckelord [en]
Ecotoxicity, Gene expression, Resistance, Sludge, Stress response, Virulence
Nationell ämneskategori
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:oru:diva-67511DOI: 10.1111/jam.14028ISI: 000447408400024PubMedID: 29928772Scopus ID: 2-s2.0-85050482911OAI: oai:DiVA.org:oru-67511DiVA, id: diva2:1224051
Forskningsfinansiär
KK-stiftelsen, Dnr 20110177Tillgänglig från: 2018-06-26 Skapad: 2018-06-26 Senast uppdaterad: 2018-11-01Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Khan, Faisal AhmadOlsson, Per-ErikJass, Jana

Sök vidare i DiVA

Av författaren/redaktören
Khan, Faisal AhmadOlsson, Per-ErikJass, Jana
Av organisationen
Institutionen för naturvetenskap och teknik
I samma tidskrift
Journal of Applied Microbiology
Mikrobiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 143 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf