Till Örebro universitet

oru.seÖrebro universitets publikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Difference in the action spectra for UVR8 monomerisation and HY5 transcript accumulation in Arabidopsis
Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Bower Building, University of Glasgow, Glasgow, UK.
Örebro universitet, Institutionen för naturvetenskap och teknik. Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Bower Building, University of Glasgow, Glasgow, UK. (Molekylär biokemi)ORCID-id: 0000-0002-8834-4064
Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden; School of Science & Technology, Örebro Life Science Center, Örebro University, Örebro, Sweden.
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Visa övriga samt affilieringar
2018 (Engelska)Ingår i: Photochemical and Photobiological Sciences, ISSN 1474-905X, E-ISSN 1474-9092, Vol. 17, nr 8, s. 1108-1117Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) activates photomorphogenic responses when plants are exposed to ultraviolet-B (UVB) light. However, whereas the absorption spectrum of UVR8 peaks at 280 nm, action spectra for several photomorphogenic UV-B responses show maximal photon effectiveness at 290-300 nm. To investigate this apparent discrepancy we measured the effectiveness of UV wavelengths in initiating two responses in Arabidopsis: photoconversion of homodimeric UVR8 into the monomeric form, which is active in signaling, and accumulation of transcripts of the ELONGATED HYPOCOTYL 5 (HY5) transcription factor, which has a key role in UVR8-mediated responses. When purified UVR8 or Arabidopsis leaf extracts were exposed to UV light monomerisation was maximal at approximately 280 nm, which correlates with the UVR8 absorption spectrum. When intact plants were exposed to UV, monomerisation was most strongly initiated at approximately 290 nm, and this shift in maximal effectiveness could be explained by strong absorption or reflectance at 280 nm by leaf tissue. Notably, the action spectrum for accumulation of HY5 transcripts in the same leaf tissue samples used to assay UVR8 dimer/monomer status peaked at approximately 300 nm. Possible reasons for the difference in maximal photon effectiveness of UVR8 monomerisation and HY5 transcript accumulation in leaf tissue are discussed.

Ort, förlag, år, upplaga, sidor
Royal Society of Chemistry, 2018. Vol. 17, nr 8, s. 1108-1117
Nationell ämneskategori
Växtbioteknologi Biokemi och molekylärbiologi
Forskningsämne
Biokemi
Identifikatorer
URN: urn:nbn:se:oru:diva-67649DOI: 10.1039/C8PP00138CISI: 000441153300013PubMedID: 29993086Scopus ID: 2-s2.0-85051272104OAI: oai:DiVA.org:oru-67649DiVA, id: diva2:1230133
Forskningsfinansiär
KK-stiftelsen, 20130164Forskningsrådet FormasCarl Tryggers stiftelse för vetenskaplig forskning
Anmärkning

Funding Agencies:

Consejo Nacional de Ciencia y Tecnologia (CONACYT)  

UK Biotechnology and Biological Sciences Research Council PhD studentship (University of Glasgow)  

Sven and Lily Lawski's Foundation for Scientific Research (University of Örebro) 

Faculty for Business, Science, and Technology at Örebro University

EU COST action 'UV4Growth'  FA0906 

University of Glasgow 

Tillgänglig från: 2018-07-03 Skapad: 2018-07-03 Senast uppdaterad: 2018-08-22Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Person

O'Hara, AndrewStrid, Åke

Sök vidare i DiVA

Av författaren/redaktören
O'Hara, AndrewStrid, Åke
Av organisationen
Institutionen för naturvetenskap och teknik
I samma tidskrift
Photochemical and Photobiological Sciences
VäxtbioteknologiBiokemi och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 998 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf