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HSP27 is not mandatory for the hypertrophy of human skeletal muscle cells
Örebro universitet, Institutionen för hälsovetenskaper.
Örebro universitet, Institutionen för medicinska vetenskaper. Department of Clinical Research Laboratory.
Örebro universitet, Institutionen för hälsovetenskaper.ORCID-id: 0000-0002-9831-0896
(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Nationell ämneskategori
Fysiologi
Identifikatorer
URN: urn:nbn:se:oru:diva-69017OAI: oai:DiVA.org:oru-69017DiVA, id: diva2:1250437
Tillgänglig från: 2018-09-24 Skapad: 2018-09-24 Senast uppdaterad: 2018-09-24Bibliografiskt granskad
Ingår i avhandling
1. Heat shock proteins in exercised human skeletal muscle
Öppna denna publikation i ny flik eller fönster >>Heat shock proteins in exercised human skeletal muscle
2018 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Exercise is considered as an important stressor accompanied by concerted molecular and cellular changes leading to adaptations at the level of skeletal muscle size and function. An important protein family produced by cells in response to stressful conditions is the heat shock proteins (HSPs). It is suggested that the different HSPs play specific roles in acute and longterm responses to exercise-induced stress. The overall aim of this thesis was to explore the expression of four different HSPs (αB-crystallin, HSP27, HSP60 and HSP70) in human skeletal muscle exposed to exercise, with a special emphasis on the role played by HSP27 in the hypertrophy of human skeletal muscle.

One of the major findings was the fibre type-specific expression of HSPs in resting human skeletal muscle, including the preferential expression of HSP27 in fast type II muscle fibres. Another finding was the occurrence of training background-related differences in the expression of HSPs. Also, a cytoplasmic relocation of HSP27, occurring specifically in type II muscle fibres, was shown in response to a single bout of resistance exercise. Interestingly, there were no corresponding changes in response to an endurance exercise bout, suggesting that HSP27 may be specifically involved in the adaptations to resistance exercise. In order to test this hypothesis, an in-vitro exercise model based on the electrical pulse stimulation (EPS) of muscle cells was developed. The EPS protocol, including an 8 h restitution period, induced a significant hypertrophy of muscle cells together with molecular changes similar to those previously described in response to exercise in humans. The role of HSP27 in the hypertrophy of human muscle cells was examined through the downregulation of HSP27. Based on data from morphological and microarray analyses, findings indicate that HSP27 is not mandatory for the hypertrophy of human muscle cells. Overall, the present thesis clarified the expression of different HSPs in human skeletal muscle and provided an in-vitro-based approach for the elucidation of the exact role played by HSPs in the adaptations of human skeletal muscle to exercise.

Ort, förlag, år, upplaga, sidor
Örebro: Örebro University, 2018. s. 56
Serie
Örebro Studies in Sport Sciences, ISSN 1654-7535 ; 28
Nyckelord
Endurance training, Resistance training, Muscle Fibre Type, Electrical Pulse Stimulation, Muscle Hypertrophy
Nationell ämneskategori
Idrottsvetenskap
Identifikatorer
urn:nbn:se:oru:diva-68028 (URN)978-91-7529-260-1 (ISBN)
Disputation
2018-10-17, Örebro universitet, Gymnastik- och idrottshuset, Hörsal G, Fakultetsgatan 1, Örebro, 09:00 (Engelska)
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Handledare
Tillgänglig från: 2018-07-23 Skapad: 2018-07-23 Senast uppdaterad: 2018-09-25Bibliografiskt granskad

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Folkesson, MattiasKruse, RobertKadi, Fawzi

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