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Tumor volume of colon carcinoma is related to the invasive pattern but not to the expression of cell adhesion proteins
Örebro universitet, Hälsoakademin.
2009 (Engelska)Ingår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 117, nr 3, s. 205-211Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Tumor volume increases during growth and due to tumor progression various mutations appear that may cause phenotypic changes. The invasive pattern may thus be affected resulting in a more disorganized growth. This phenomenon might be due to mutations in the genome of the adhesion proteins, which are responsible for the structural integrity of epithelial tissue. Tumor volume was assessed in whole mount sections of 33 colon carcinomas using Cavalieri's principle. Images from the entire invasive border were captured and used for calculating the irregularity of the border (Complexity Index). The expression of the adhesion proteins E-cadherin, beta-catenin, Claudin 2 and Occludin was assessed after immunohistochemical staining of two randomly selected areas of the invasive front of the tumor. Statistical significance for differences in volume was obtained for tumor Complexity Index, tumor stage (pT) and lymph node status (pN). Expression of adhesion proteins was significantly perturbed in the tumors compared with normal mucosa but only infrequently correlated to tumor differentiation or invasive pattern. The results show that when tumor volume increases the invasive pattern becomes more irregular which is compatible with tumor progression. A direct contribution of adhesion protein derangement to this process appears to be insignificant.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell Publishing Inc., 2009. Vol. 117, nr 3, s. 205-211
Nationell ämneskategori
Medicin och hälsovetenskap
Forskningsämne
Biomedicin
Identifikatorer
URN: urn:nbn:se:oru:diva-3033DOI: 10.1111/j.1600-0463.2008.00011.xISI: 000265487600006PubMedID: 19245593Scopus ID: 2-s2.0-61349175837OAI: oai:DiVA.org:oru-3033DiVA, id: diva2:136461
Tillgänglig från: 2008-11-10 Skapad: 2008-11-10 Senast uppdaterad: 2017-12-14Bibliografiskt granskad
Ingår i avhandling
1. Cell adhesion proteins in different invasive patterns of colon carcinomas: a morphometric and molecular genetic study
Öppna denna publikation i ny flik eller fönster >>Cell adhesion proteins in different invasive patterns of colon carcinomas: a morphometric and molecular genetic study
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Colorectal carcinoma is the second most common type of cancer in both men and women in Sweden. Cancer of the colon and rectum are often considered together and their ten year survival rate is approximately 50 – 60 % depending on sex and location. Different histopathological characteristics of such cancers, including the complexity of growth, are of importance for prognosis.

This thesis has compared different morphometric methods in order to achieve a quantitative and objective measurement of the invasive front of colon carcinoma. Since the growth pattern is dependent on the cell adhesiveness of different proteins we studied the distribution and localization of E-cadherin, Beta-catenin, Claudin 1,2,7 and Occludin as well as screened the genes for mutations.

We found a perturbed protein expression of E-cadherin, Beta-catenin, Claudin 1,2,7 and Occludin in tumor sections compared to normal mucosa, but no relation to tumor volume or growth pattern could be seen. The tumor volume was found to be correlated to the growth pattern but not responsible to the perturbed protein expression. In the mutation screening we found a SNP in exon 13 the E-cadherin gene in the tumor, as well as in exon 2 of Claudin 1 and exon 4 of Claudin 7 in both tumor and normal mucosa. No correlation between mutations and growth pattern or tumor volume was found.

In conclusion, this thesis shows that the computer image analysis with estimation of fractal dimension and number of free tumor cell clusters is superior to the semi quantitative visual grading of tumor invasive complexity. The aberrant expression of cell adhesion proteins in the tumor compared to normal mucosa as well as polymorphisms in the cell adhesion genes CLDN1 and CLDN7 in both tumor and normal mucosa can suggest that these aberrations are important in the tumorigenesis of colon carcinoma.

 

Ort, förlag, år, upplaga, sidor
Örebro: Örebro universitet, 2008. s. 61
Serie
Örebro Studies in Medicine, ISSN 1652-4063 ; 24
Nyckelord
colon carcinoma, growth pattern, tight junction, Complexity Index, cell adhesion, E-cadherin, Beta-catenin, Occludin, Claudin.
Nationell ämneskategori
Klinisk vetenskap
Forskningsämne
Biomedicin
Identifikatorer
urn:nbn:se:oru:diva-2603 (URN)978-91-7668-640-9 (ISBN)
Disputation
2008-11-28, Wilandersalen, USÖ, Universitetsjukhuset, Örebro, 09:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2008-11-10 Skapad: 2008-11-10 Senast uppdaterad: 2017-10-18Bibliografiskt granskad

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Hahn-Strömberg, VictoriaBodin, Lennart

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