oru.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Skin barrier disruption by sodium lauryl sulfate-exposure alters the expressions of involucrin, transglutaminase 1, profilaggrin, and kallikreins during the repair phase in human skin in vivo
Örebro universitet, Hälsoakademin.
2008 (Engelska)Ingår i: Journal of Investigative Dermatology, ISSN 0022-202X, E-ISSN 1523-1747, Vol. 128, nr 5, s. 1212-1219Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Detergents are skin irritants affecting keratinocytes. In this study, healthy volunteers were exposed to water (vehicle) and 1% sodium lauryl sulfate (SLS) under occlusive patch tests for 24 hours. The messenger RNA (mRNA) expression of keratinocyte differentiation markers and of enzymes involved in corneodesmosome degradation was examined in skin biopsies (n=8) during the repair phase (6 hours to 7 days postexposure) using real-time reverse-transcription PCR. It was found that the expression of involucrin was increased at 6 hours, but then rapidly normalized. The expression of transglutaminase 1 exhibited a twofold increase after 24 hours in the SLS-exposed skin. Profilaggrin was decreased after 6 hours. Later (4–7 days), the expression in SLS-exposed areas was >50% above than in control areas. An increased and altered immunofluorescence pattern of involucrin, transglutaminase 1, and filaggrin was also found (n=4). At 6 hours post-SLS exposure, the mRNA expression of kallikrein-7 (KLK-7) and kallikrein-5 (KLK-5) was decreased by 50 and 75%, respectively, as compared with control and water-exposed areas. Thereafter, the expression pattern of KLK-7 and KLK-5 was normalized. Changes in protein expression of KLK-5 were also found. In conclusion, SLS-induced skin barrier defects induce altered mRNA expression of keratinocyte differentiation markers and enzymes degrading corneodesmosomes.

Ort, förlag, år, upplaga, sidor
London: Nature publishing group , 2008. Vol. 128, nr 5, s. 1212-1219
Nyckelord [en]
Adult, Biopsy, Cell Differentiation/drug effects, Dermatitis; Irritant/pathology/*physiopathology, Female, Humans, Intermediate Filament Proteins/*genetics/metabolism, Irritants/toxicity, Kallikreins/*genetics/metabolism, Keratinocytes/drug effects/metabolism/pathology, Male, Middle Aged, Patch Tests, Protein Precursors/*genetics/metabolism, RNA; Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Skin/metabolism/pathology/physiopathology, Sodium Dodecyl Sulfate/toxicity, Transglutaminases/*genetics/metabolism, Wound Healing/physiology
Nationell ämneskategori
Matematik Dermatologi och venereologi
Forskningsämne
Dermatologi och venereologi
Identifikatorer
URN: urn:nbn:se:oru:diva-3448DOI: 10.1038/sj.jid.5701170PubMedID: 18007579OAI: oai:DiVA.org:oru-3448DiVA, id: diva2:137745
Tillgänglig från: 2008-12-08 Skapad: 2008-12-08 Senast uppdaterad: 2017-12-14Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=18007579&dopt=Citation

Personposter BETA

Lindberg, Magnus

Sök vidare i DiVA

Av författaren/redaktören
Lindberg, Magnus
Av organisationen
Hälsoakademin
I samma tidskrift
Journal of Investigative Dermatology
MatematikDermatologi och venereologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 30 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf