oru.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Zebrafish androgen receptor: isolation, molecular, and biochemical characterization
Örebro universitet, Akademin för naturvetenskap och teknik.
Örebro universitet, Akademin för naturvetenskap och teknik.ORCID-id: 0000-0001-9713-2365
Örebro universitet, Akademin för naturvetenskap och teknik.ORCID-id: 0000-0001-7336-6335
Visa övriga samt affilieringar
2008 (Engelska)Ingår i: Biology of Reproduction, ISSN 0006-3363, E-ISSN 1529-7268, Vol. 78, nr 2, s. 361-369Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Androgens play an important role in male sexual differentiation and development. They exert their function by binding to and activating the androgen receptor (Ar), a member of the steroid hormone receptor superfamily. Here, we report on the isolation and characterization of zebrafish Ar. The complete transcript of zebrafish ar is 5.3 kb long encoding a putative polypeptide of 868 amino acids. Our experimental and bioinformatic analysis has found a single ar locus in zebrafish. Phylogenetic analysis using the ligand-binding domain showed that the zebrafish Ar clustered with its cyprinid orthologs to form a separate group, which was closer to the beta clade than to the alpha clade. Tissue-specific expression analysis revealed that the ar mRNA was expressed ubiquitously in all adult tissues tested, with sexually dimorphic expression in the gonad and muscle. While the ar transcript was maternally deposited into the embryo, signs of zygotic expression could be detected as early as 24 h after fertilization, and the expression level increased substantially afterwards. When analyzed during gonad development, the expression level of ar mRNA at 4 wk after fertilization was similar in both developing gonads but later became higher in the transforming testis, suggesting a potential role during male gonad differentiation. We also combined theoretical modeling with in vitro experiments to show that the zebrafish Ar is preferentially activated by 11-ketotestosterone.

Ort, förlag, år, upplaga, sidor
Champaign, Ill.: Society for the Study of Reproduction , 2008. Vol. 78, nr 2, s. 361-369
Nyckelord [en]
Androgens/*metabolism/pharmacology, Animals, Chromosome Mapping, Cloning; Molecular, Computer Simulation, DNA; Complementary/genetics, Female, Gene Expression, Gonads/growth & development, Male, Molecular Sequence Data, Phylogeny, RNA; Messenger/metabolism, Receptors; Androgen/agonists/*classification/*genetics, Sequence Homology; Amino Acid, Testosterone/analogs & derivatives/pharmacology, Transcription; Genetic, Transfection, Zebrafish/genetics/*growth & development, Zebrafish Proteins/*genetics
Nationell ämneskategori
Naturvetenskap Biologiska vetenskaper
Forskningsämne
biologi
Identifikatorer
URN: urn:nbn:se:oru:diva-3557DOI: 10.1095/​biolreprod.107.062018PubMedID: 17942797OAI: oai:DiVA.org:oru-3557DiVA, id: diva2:137854
Tillgänglig från: 2008-12-09 Skapad: 2008-12-09 Senast uppdaterad: 2017-12-14Bibliografiskt granskad
Ingår i avhandling
1. Androgen receptors and endocrine disrupting substances
Öppna denna publikation i ny flik eller fönster >>Androgen receptors and endocrine disrupting substances
2010 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Throughout the animal kingdom, organisms are dependent on substances such as steroid hormones to help them maintain internal balances. Examples of important tasks that are under regulation of steroid hormones are somatic and gonadal development, sexual performance and behavior (both social and sexual) as well as sex differentiation. Balance in the biology of reproduction is important for all organisms, and is sensitive to alterations and disturbances. If the environment is altered in a manner that lead to higher estrogenic or androgenic levels, the sex ratio of organisms that do not rely on genetic differences in the sex differentiation, will be biased towards more females or males in the population. It has been known for some time that there are pollutants in the environment that affect steroid pathways, such as the estrogenic and thyroid systems, but not much has been known about the androgenic systems. Examples of populations being masculinized have been recorded, and estrogenic compounds have been known to act as antiandrogens, but not until recently the first androgen agonist was identified. We used a combination of in vitro and computational modeling to identify the brominated flame retardant, 1,2-dibromo-4-(1,2-dibromoethyl)cyclohexane, as a potent androgen agonist to the human androgen receptor.

In addition to this we cloned and characterized the androgen receptor from, a frequently used model organism, zebrafish (Danio rerio) as a receptor primarily activated by 11-ketotestosterone. This is a feature the zebrafish share with several other teleost fishes, such as the three-spined stickleback. Thus fish androgen receptors differ from most mammalian androgen receptors, where dihydrotestosterone is the most potent activator.

 

Ort, förlag, år, upplaga, sidor
Örebro: Örebro universitet, 2010. s. 64
Serie
Örebro Studies in Life Science ; 5
Nyckelord
Androgen receptor, Brominated flame retardant, TBECH, Endocrine disruptor
Nationell ämneskategori
Biologiska vetenskaper Naturvetenskap
Forskningsämne
Biologi
Identifikatorer
urn:nbn:se:oru:diva-9679 (URN)978-91-7668-714-7 (ISBN)
Disputation
2010-02-26, Hörsal P2, Örebro, 10:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2010-02-10 Skapad: 2010-02-09 Senast uppdaterad: 2017-10-18Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17942797&dopt=Citation

Personposter BETA

Larsson, AndersScherbak, NikolaiOlsson, Per-Erik

Sök vidare i DiVA

Av författaren/redaktören
Larsson, AndersScherbak, NikolaiOlsson, Per-Erik
Av organisationen
Akademin för naturvetenskap och teknik
I samma tidskrift
Biology of Reproduction
NaturvetenskapBiologiska vetenskaper

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 503 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf