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Platelet-derived growth factor increases the turnover of GTP/GDP on Ras in permeabilized fibroblasts
Department of Pathology, University Hospital, Uppsala, Sweden.ORCID-id: 0000-0001-8889-5803
Department of Pathology, University Hospital, Uppsala, Sweden.
1993 (Engelska)Ingår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 268, nr 24, s. 18187-18194Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The potent mitogen platelet-derived growth factor (PDGF) induced a rapid increase in Ras.GTP in permeabilized human and murine fibroblasts. The effect was initiated by both PDGF-AA acting exclusively through PDGF alpha-receptors, and by PDGF-BB interacting with both alpha- and beta-type receptors. The dose-response curves suggest that both receptor types mediate the response. PDGF-dependent Ras activation, measured as increased formation of Ras.GTP, was rapid and reversible. At 37 degrees C the effect had a duration of around 10 min. The PDGF-dependent increase in Ras.GTP was followed by a simultaneous increase in Ras.GDP. Under no experimental condition could a relative increase in Ras.GTP be detected. 0.5 microM GDP and 0.5 microM GTP were equally potent competing for the formation of Ras.[alpha-32P]GTP upon PDGF stimulation. Furthermore, when the basal nucleotide exchange rate on Ras was elevated by omission of Mg2+ from the medium, PDGF had no further effect on the formation of Ras.GTP. We therefore conclude that PDGF activates Ras through a mechanism leading to an increased nucleotide exchange on Ras.

Ort, förlag, år, upplaga, sidor
The American Society for Biochemistry and Molecular Biology, Inc. , 1993. Vol. 268, nr 24, s. 18187-18194
Nationell ämneskategori
Medicin och hälsovetenskap Farmakologi och toxikologi
Identifikatorer
URN: urn:nbn:se:oru:diva-79049ISI: A1993LT74300080PubMedID: 8349694Scopus ID: 2-s2.0-0027219341OAI: oai:DiVA.org:oru-79049DiVA, id: diva2:1386270
Forskningsfinansiär
Magnus Bergvalls Stiftelse
Anmärkning

This work was supported by the Swedish Natural Science Research Council and Magn. Bergvalls Stiftelse (to E. N.).

Tillgänglig från: 2013-10-21 Skapad: 2020-01-17 Senast uppdaterad: 2020-01-27Bibliografiskt granskad

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PubMedScopusPlatelet-derived growth factor increases the turnover of GTP/GDP on Ras in permeabilized fibroblasts

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