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Immunocytochemical localization of glucocorticoid receptor in human gingival fibroblasts and evidence for a colocalization of glucocorticoid receptor with cytoplasmic microtubules
Örebro universitet, Hälsoakademin.ORCID-id: 0000-0002-1592-8835
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1990 (Engelska)Ingår i: European Journal of Cell Biology, ISSN 0171-9335, E-ISSN 1618-1298, Vol. 53, nr 2, s. 390-401Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The cellular distribution of the glucocorticoid receptor (GR) in relation to various intracellular and plasma membrane structures in human fibroblasts was studied using indirect immunofluorescence techniques with monoclonal and polyclonal antibodies. During interphase, GR was located predominantly in the cytoplasm, showing a similar pattern as tubulin. In mitotic cells, GR and tubulin were localized in mitotic spindles and in telophase midbodies. Colchicine and vinblastine induced a similar redistribution of GR and tubulin to the cell periphery. This redistribution was reversible for colchicine but not for vinblastine. Vinblastine also induced paracrystals containing GR and tubulin. These results support the hypothesis that GR interacts in vivo with cytoplasmic microtubules.

Ort, förlag, år, upplaga, sidor
1990. Vol. 53, nr 2, s. 390-401
Nationell ämneskategori
Medicin och hälsovetenskap
Forskningsämne
Cellforskning
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URN: urn:nbn:se:oru:diva-20229PubMedID: 2081553OAI: oai:DiVA.org:oru-20229DiVA, id: diva2:452870
Tillgänglig från: 2011-10-31 Skapad: 2011-10-29 Senast uppdaterad: 2017-12-08Bibliografiskt granskad

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