oru.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
A quantitative study of the mechanisms behind thymic atrophy in G alpha i2-deficient mice during colitis development
Örebro universitet, Institutionen för hälsovetenskap och medicin.
Systems Biology Research Centre, University of Skövde, Skövde, Sweden.
Örebro universitet, Institutionen för läkarutbildning.ORCID-id: 0000-0001-5460-8888
2012 (Engelska)Ingår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, nr 5, artikel-id e36726Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Mice deficient for the G protein subunit G alpha i2 spontaneously develop colitis, a chronic inflammatory disease associated with dysregulated T cell responses. We and others have previously demonstrated a thymic involution in these mice and an aberrant thymocyte dynamics. The G alpha i2(-/-) mice have a dramatically reduced fraction of double positive thymocytes and an increased fraction of single positive (SP) thymocytes. In this study, we quantify a number of critical parameters in order to narrow down the underlying mechanisms that cause the dynamical changes of the thymocyte development in the G alpha i2(-/-) mice. Our data suggest that the increased fraction of SP thymocytes results only from a decreased number of DP thymocytes, since the number of SP thymocytes in the Gai2(-/-) mice is comparable to the control littermates. By measuring the frequency of T cell receptor excision circles (TRECs) in the thymocytes, we demonstrate that the number of cell divisions the G alpha i2(-/-) SP thymocytes undergo is comparable to SP thymocytes from control littermates. In addition, our data show that the mature SP CD4(+) and CD8(+) thymocytes divide to the same extent before they egress from the thymus. By estimating the number of peripheral TREC+ T lymphocytes and their death rate, we could calculate the daily egression of thymocytes. G alpha i2(-/-) mice with no/mild and moderate colitis were found to have a slower export rate in comparison to the control littermates. The quantitative measurements in this study suggest a number of dynamical changes in the thymocyte development during the progression of colitis.

Ort, förlag, år, upplaga, sidor
San Francisco, USA: Public Library of Science , 2012. Vol. 7, nr 5, artikel-id e36726
Nationell ämneskategori
Immunologi inom det medicinska området
Forskningsämne
Biomedicin
Identifikatorer
URN: urn:nbn:se:oru:diva-24419DOI: 10.1371/journal.pone.0036726ISI: 000305336400031PubMedID: 22590596Scopus ID: 2-s2.0-84861002198OAI: oai:DiVA.org:oru-24419DiVA, id: diva2:544541
Forskningsfinansiär
VetenskapsrådetKK-stiftelsen
Anmärkning

Funding Agencies:

Swedish Cancer Society 

Foundation of Professor Nanna Svartz

Örebro University 

Swedish Society of Medicine 

Tillgänglig från: 2012-08-15 Skapad: 2012-08-15 Senast uppdaterad: 2018-01-12Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Elgbratt, KristinaHultgren-Hörnquist, Elisabeth

Sök vidare i DiVA

Av författaren/redaktören
Elgbratt, KristinaHultgren-Hörnquist, Elisabeth
Av organisationen
Institutionen för hälsovetenskap och medicinInstitutionen för läkarutbildning
I samma tidskrift
PLoS ONE
Immunologi inom det medicinska området

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 496 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf