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Unlocking pathology archives for microRNA-profiling
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
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2008 (Engelska)Ingår i: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 28, nr 1A, s. 119-23Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: MicroRNAs (miRNAs) are approximately 22 nucleotide long, non-coding RNAs that regulate gene expression by binding to the 3'-untranslated region of target mRNAs and also a variety of cellular processes. It has recently been established that dysregulation of miRNA expression can be detected in the majority of human cancers. A variety of high-throughput screening methods has been developed to identify dysregulated miRNA species in tumours. For retrospective clinical studies formalin-fixed, paraffin-embedded (FFPE) tissue is the most widely used material.

Materials and methods: The miRNA expression profiles of freshly frozen (CRYO) and FFPE tissues of seven tonsil and four liver samples were compared, using a qPCR-based assay, profiling 157 miRNA species.

Results: The significance of miRNA-profiles was barely influenced by FFPE treatment in both tissues and the variance induced by FFPE treatment was much smaller than the variance caused by biologically based differential expression.

Conclusion: FFPE material is well suited for miRNA profiling.

Ort, förlag, år, upplaga, sidor
Athens, Greece: International Institute of Anticancer Research, 2008. Vol. 28, nr 1A, s. 119-23
Nyckelord [en]
miRNA, FFPE, expression profiling
Nationell ämneskategori
Bioinformatik och systembiologi
Identifikatorer
URN: urn:nbn:se:oru:diva-40636ISI: 000253844200017PubMedID: 18383833Scopus ID: 2-s2.0-40549095349OAI: oai:DiVA.org:oru-40636DiVA, id: diva2:778025
Tillgänglig från: 2015-01-09 Skapad: 2015-01-09 Senast uppdaterad: 2018-01-30Bibliografiskt granskad

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