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Time-dependent expression and activity of cytochrome P450 1s in early life-stages of the zebrafish (Danio rerio)
Institute for Environmental Research, RWTH Aachen University, Aachen, Germany; National Research Centre for Environmental Toxicology (Entox), The University of Queensland, Brisbane, Australia.
Institute for Environmental Research, RWTH Aachen University, Aachen, Germany.
Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Wildau, Germany.
Institute for Environmental Research, RWTH Aachen University, Aachen, Germany.
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2015 (Engelska)Ingår i: Environmental science and pollution research international, ISSN 0944-1344, E-ISSN 1614-7499, Vol. 22, nr 21, s. 16319-15328Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Zebrafish embryos are being increasingly used as model organisms for the assessment of single substances and complex environmental samples for regulatory purposes.Thus, it is essential to fully understand the xenobiotic metabolism during the different life-stages of early development.The aim of the present study was to determine arylhydrocarbon receptor (AhR)-mediated activity during selected times of early development using qPCR, enzymatic activity through measurement of 7-ethoxyresorufin-Odeethylase(EROD) activity, and protein expression analysis. In the present study, gene expression of cyp1a, cyp1b1, cyp1c1, cyp1c2, and ahr2 as well as EROD activity were investigated up to 120 h postfertilization (hpf) after exposure to either β-naphthoflavone (BNF) or a polycyclic aromatichydrocarbons (PAH)-contaminated sediment extract from Vering Kanal in Hamburg (VK). Protein expression was measured at 72 hpf after exposure to 20 μg/L BNF. Altered proteins were identified by matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) peptide mass fingerprinting. Distinct patterns of basal messenger RNA (mRNA) expressionwere found for each of the cyp1 genes, suggesting specific roles during embryonic development. All transcripts were induced by BNF and VK. ahr2 mRNA expression was significantly upregulated after exposure toVK. All cyp1 genes investigated showed a temporal decline in expression at 72 hpf. The significant decline of Hsp 90β protein at 72 hpf after exposure to BNF may suggest an explanation for the decline of cyp1 genes at this time point as Hsp 90β is of major importance for the functioning of the Ah-receptor. EROD activity measured in embryos was significantly induced after 96 hpf of exposure to BNF or VK. Together, these results demonstrate distinct temporal patterns of cyp1 genes and protein activities in zebrafish embryos as well as show a need to investigate further the xenobiotic biotransformation system during early development of zebrafish.

Ort, förlag, år, upplaga, sidor
Springer, 2015. Vol. 22, nr 21, s. 16319-15328
Nyckelord [en]
Arylhydrocarbon receptor, β-naphthoflavone, Cytochrome P450, EROD, Hsp 90β, Zebrafish
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URN: urn:nbn:se:oru:diva-44706DOI: 10.1007/s11356-015-4673-6ISI: 000363964700007PubMedID: 25994265Scopus ID: 2-s2.0-84945443216OAI: oai:DiVA.org:oru-44706DiVA, id: diva2:814677
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Funding Agency:

German Federal Ministry of Education and Research 02WU1053

Tillgänglig från: 2015-05-27 Skapad: 2015-05-27 Senast uppdaterad: 2017-12-04Bibliografiskt granskad

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Keiter, Steffen

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