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Comparative genome analysis of Lysinibacillus B1-CDA, a bacterium that accumulates arsenics
Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden; School of Science and Technology, Örebro University, Örebro, Sweden. (The Life Science Center)ORCID-id: 0000-0002-8162-8945
Systems Biology Research Center, School of Bioscience, University of Skövde, Skövde, Sweden.
Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune, India.
Örebro universitet, Institutionen för naturvetenskap och teknik.ORCID-id: 0000-0001-7957-0310
Visa övriga samt affilieringar
2015 (Engelska)Ingår i: Genomics, ISSN 0888-7543, E-ISSN 1089-8646, Vol. 106, nr 6, s. 384-392Artikel i tidskrift (Refereegranskat) Published
Resurstyp
Text
Abstract [en]

Previously, we reported an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, isolated from an arsenic contaminated lands. Here, we have investigated its genetic composition and evolutionary history by using massively parallel sequencing and comparative analysis with other known Lysinibacillus genomes. Assembly of the sequencing reads revealed a genome of similar to 4.5 Mb in size encompassing similar to 80% of the chromosomal DNA. We found that the set of ordered contigs contains abundant regions of similarity with other Lysinibacillus genomes and clearly identifiable genome rearrangements. Furthermore, all genes of B1-CDA that were predicted be involved in its resistance to arsenic and/or other heavy metals were annotated. The presence of arsenic responsive genes was verified by PCR in vitro conditions. The findings of this study highlight the significance of this bacterium in removing arsenics and other toxic metals from the contaminated sources. The genetic mechanisms of the isolate could be used to cope with arsenic toxicity.

Ort, förlag, år, upplaga, sidor
Academic Press, 2015. Vol. 106, nr 6, s. 384-392
Nyckelord [en]
Toxic metals, Bioremediation, Lysinibacillus sphaericus B1-CDA, Genome sequencing, de novo assembly, Gene prediction
Nationell ämneskategori
Miljövetenskap Miljöbioteknik
Forskningsämne
Miljövetenskap
Identifikatorer
URN: urn:nbn:se:oru:diva-47292DOI: 10.1016/j.ygeno.2015.09.006ISI: 000365613100010PubMedID: 26387925Scopus ID: 2-s2.0-84948102629OAI: oai:DiVA.org:oru-47292DiVA, id: diva2:890940
Anmärkning

Funding Agencies:

Swedish International Development Cooperation Agency (SIDA) AKT-2010-018

Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS) 229-2007-217

Nilsson-Ehle (The Royal Physio-graphic Society in Lund) foundation in Sweden

Tillgänglig från: 2016-01-05 Skapad: 2016-01-04 Senast uppdaterad: 2018-12-07Bibliografiskt granskad
Ingår i avhandling
1. Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
Öppna denna publikation i ny flik eller fönster >>Bioremediation of Toxic Metals for Protecting Human Health and the Ecosystem
2016 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Heavy metal pollutants, discharged into the ecosystem as waste by anthropogenic activities, contaminate drinking water for millions of people and animals in many regions of the world. Long term exposure to these metals, leads to several lethal diseases like cancer, keratosis, gangrene, diabetes, cardio- vascular disorders, etc. Therefore, removal of these pollutants from soil, water and environment is of great importance for human welfare. One of the possible eco-friendly solutions to this problem is the use of microorganisms that can accumulate the heavy metals from the contaminated sources, hence reducing the pollutant contents to a safe level.

In this thesis an arsenic resistant bacterium Lysinibacillus sphaericus B1-CDA, a chromium resistant bacterium Enterobacter cloacae B2-DHA and a nickel resistant bacterium Lysinibacillus sp. BA2 were isolated and studied. The minimum inhibitory concentration values of these isolates are 500 mM sodium arsenate, 5.5 mM potassium chromate and 9 mM nickel chloride, respectively. The time of flight-secondary ion mass spectrometry and inductively coupled plasma-mass spectroscopy analyses revealed that after 120 h of exposure, the intracellular accumulation of arsenic in B1-CDA and chromium in B2-DHA were 5.0 mg/g dwt and 320 μg/g dwt of cell biomass, respectively. However, the arsenic and chromium contents in the liquid medium were reduced to 50% and 81%, respectively. The adsorption values of BA2 when exposed to nickel for 6 h were 238.04 mg of Ni(II) per gram of dead biomass indicating BA2 can reduce nickel content in the solution to 53.89%. Scanning electron micrograph depicted the effect of these metals on cellular morphology of the isolates. The genetic composition of B1-CDA and B2-DHA were studied in detail by sequencing of whole genomes. All genes of B1-CDA and B2-DHA predicted to be associated with resistance to heavy metals were annotated.

The findings in this study accentuate the significance of these bacteria in removing toxic metals from the contaminated sources. The genetic mechanisms of these isolates in absorbing and thus removing toxic metals could be used as vehicles to cope with metal toxicity of the contaminated effluents discharged to the nature by industries and other human activities.

Ort, förlag, år, upplaga, sidor
Örebro: Örebro university, 2016. s. 80
Serie
Örebro Studies in Life Science ; 15
Nyckelord
Heavy Metals, Pollution, Accumulation, Remediation, Human Health, Bacteria, Genome Sequencing, de novo Assembly, Gene Prediction
Nationell ämneskategori
Annan biologi
Forskningsämne
Biologi
Identifikatorer
urn:nbn:se:oru:diva-51436 (URN)978-91-7529-146-8 (ISBN)
Disputation
2016-09-22, Högskolan i Skövde, G-building, lecture hall G111, Högskolevägen 28, Skövde, 13:15 (Engelska)
Opponent
Handledare
Tillgänglig från: 2016-07-25 Skapad: 2016-07-25 Senast uppdaterad: 2018-12-07Bibliografiskt granskad

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