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Dual action of bacteriocin PLNC8 alpha beta through inhibition of Porphyromonas gingivalis infection and promotion of cell proliferation
Örebro universitet, Institutionen för medicinska vetenskaper.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Örebro universitet, Institutionen för medicinska vetenskaper. Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
School of Medical Sciences, Örebro University, Örebro, Sweden.
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2017 (Engelska)Ingår i: Pathogens and Disease, E-ISSN 2049-632X, Vol. 75, nr 5, artikel-id ftx064Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Periodontitis is a chronic inflammatory disease that is characterised by accumulation of pathogenic bacteria, including Porphyromonas gingivalis, in periodontal pockets. The lack of effective treatments has emphasised in an intense search for alternative methods to prevent bacterial colonisation and disease progression. Bacteriocins are bacterially produced antimicrobial peptides gaining increased consideration as alternatives to traditional antibiotics. We show rapid permeabilisation and aggregation of P. gingivalis by the two-peptide bacteriocin PLNC8 alpha beta. In a cell culture model, P. gingivalis was cytotoxic against gingival fibroblasts. The proteome profile of fibroblasts is severely affected by P. gingivalis, including induction of the ubiquitin-proteasome pathway. PLNC8 alpha beta enhanced the expression of growth factors and promoted cell proliferation, and suppressed proteins associated with apoptosis. PLNC8 alpha beta efficiently counteracted P. gingivalis-mediated cytotoxicity, increased expression of a large number of proteins and restored the levels of inflammatory mediators. In conclusion, we show that bacteriocin PLNC8 alpha beta displays dual effects by acting as a potent antimicrobial agent killing P. gingivalis and as a stimulatory factor promoting cell proliferation. We suggest preventive and therapeutical applications of PLNC8 alpha beta in periodontitis to supplement the host immune defence against P. gingivalis infection and support wound healing processes.

Ort, förlag, år, upplaga, sidor
Oxford University Press, 2017. Vol. 75, nr 5, artikel-id ftx064
Nyckelord [en]
Porphyromonas gingivalis, periodontitis, cell proliferation, proteomics, bacteriocin, PLNC8
Nationell ämneskategori
Immunologi inom det medicinska området Infektionsmedicin Mikrobiologi inom det medicinska området
Identifikatorer
URN: urn:nbn:se:oru:diva-59311DOI: 10.1093/femspd/ftx064ISI: 000407245300014Scopus ID: 2-s2.0-85028656048OAI: oai:DiVA.org:oru-59311DiVA, id: diva2:1136155
Forskningsfinansiär
KK-stiftelsen, 20150244 20150086
Anmärkning

Funding Agency:

Foundation of Magnus Bergvall 2015-00823  

Tillgänglig från: 2017-08-25 Skapad: 2017-08-25 Senast uppdaterad: 2018-01-13Bibliografiskt granskad

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Bengtsson, TorbjörnSelegård, RobertKhalaf, Hazem

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Pathogens and Disease
Immunologi inom det medicinska områdetInfektionsmedicinMikrobiologi inom det medicinska området

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