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Impact of Proinflammatory Cytokines on the Virulence of Uropathogenic Escherichia coli
School of Medical Sciences, Örebro University, Örebro, Sweden.
Örebro University, School of Medical Sciences. Nutrition-Gut-Brain Interactions Research Centre.ORCID iD: 0000-0003-3383-9219
Örebro University, School of Medical Sciences. iRiSC - Inflammatory Response and Infection Susceptibility Centre.
2019 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 10, article id 1051Article in journal (Refereed) Published
Abstract [en]

The effect of a urinary tract infection on the host is a well-studied research field. However, how the host immune response affects uropathogenic Escherichia coli (CFT073) virulence is less studied. The aim of the present study was to investigate the impact of proinflammatory cytokine exposure on the virulence of uropathogenic Escherichia coli. We found that all tested proinflammatory cytokines (TNF-alpha, IL-1 beta, IL-6, IL-8 and IFN-gamma) induced an increased CFT073 growth. We also found that biofilm formation and hemolytic activity was reduced in the presence of all proinflammatory cytokines. However, a reduction in siderophore release was only observed in the presence of IL-1 beta, IL-6 and IL-8. Real time-qPCR showed that all proinflammatory cytokines except TNF-alpha significantly increased genes associated with the iron acquisition system in CFT073. We also found that the proinflammatory cytokines induced significant changes in type-1 fimbriae, P-fimbriae and gluconeogenetic genes. Furthermore, we also showed, using a Caenorhabditis elegans (C. elegans) killing assay that all cytokines decreased the survival of C. elegans worms significantly. Taken together, our findings show that proinflammatory cytokines have the ability to alter the virulence traits of UPEC.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2019. Vol. 10, article id 1051
Keywords [en]
uropathogenic Escherichia coli, proinflammatory cytokines, cross kingdom interaction, virulence, bacterial growth
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-74408DOI: 10.3389/fmicb.2019.01051ISI: 000467422200001OAI: oai:DiVA.org:oru-74408DiVA, id: diva2:1318557
Note

Funding Agency:

Faculty of Medicine and Health at Örebro University

Available from: 2019-05-28 Created: 2019-05-28 Last updated: 2019-05-28Bibliographically approved

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Rangel, IgnacioDemirel, Isak

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