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The Gut Microbiota in Collagenous Colitis Shares Characteristics With Inflammatory Bowel Disease-Associated Dysbiosis
Örebro University, School of Medical Sciences. Department of Internal Medicine, Ersta Hospital, Stockholm, Sweden; Department of Gastroenterology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Örebro University, School of Medical Sciences.ORCID iD: 0000-0003-3887-9519
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2019 (English)In: Clinical and Translational Gastroenterology, E-ISSN 2155-384X, Vol. 10, no 7, article id e00065Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: In inflammatory bowel disease (IBD), an aberrant immune response to gut microbiota is important, but the role of the microbiota in collagenous colitis (CC) is largely unknown. We aimed to characterize the microbiota of patients with CC compared with that of healthy control and patients with IBD.

METHODS: Fecal samples were collected from patients with CC (n = 29), age- and sex-matched healthy controls (n = 29), patients with Crohn's disease (n = 32), and patients with ulcerative colitis (n = 32). Sequence data were obtained by 454 sequencing of 16S rRNA gene amplicons, and the obtained sequences were subsequently taxonomically classified.

RESULTS: Analysis of similarity statistics showed a segregation between patients with CC and healthy controls with increasing taxonomic resolution, becoming significant comparing operational taxonomic unit data (P = 0.006). CC had a lower abundance of 10 different taxa. Taxa-specific analyses revealed a consistent lower abundance of several operational taxonomic units belonging to the Ruminococcaceae family in patients with CC, q < 0.05 after false discovery rate correction. Loss of these taxa was seen in patients with CC with active disease and/or corticosteroid treatment only and resembled the findings in patients with IBD.

DISCUSSION: CC is associated with a specific fecal microbiome seen primarily in patients with active disease or ongoing corticosteroid treatment, whereas the microbiome of CC patients in remission resembled that of healthy controls. Notably, the shift in key taxa, including the Ruminococcaceae family, was also observed in IBD. There may be common mechanisms in the pathogenesis of CC and IBD.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019. Vol. 10, no 7, article id e00065
National Category
Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:oru:diva-75573DOI: 10.14309/ctg.0000000000000065ISI: 000478837900001PubMedID: 31343467Scopus ID: 2-s2.0-85070852572OAI: oai:DiVA.org:oru-75573DiVA, id: diva2:1341658
Available from: 2019-08-09 Created: 2019-08-09 Last updated: 2023-12-08Bibliographically approved
In thesis
1. Chronic inflammatory bowel diseases: studies of microbiota and its influence
Open this publication in new window or tab >>Chronic inflammatory bowel diseases: studies of microbiota and its influence
2021 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Introduction: Inflammatory bowel diseases are becoming increasingly common. The underlying mechanisms are not entirely known but the gut microbiota seem to be involved in the pathogenesis. 

Aim: The aim of this thesis was to characterise gut microbiota related to diagnosis, disease course and response to biological treatment, taking aspects of the source of biological material into account. 

Materials and methods: Patients and healthy individuals from several different cohorts in Sweden and Europe were invited. Faecal samples and mucosal biopsies were analysed using different sequencing platforms to investigate the gut microbiota. In Study I the faecal microbiota was correlated to different inflammatory bowel diseases. In Study II we compared the microbiota in faeces to the microbiota in mucosal biopsies. In StudyIII we related the faecal microbiota to the outcome of biological treatment. In Study IV we investigated the diagnostic and prognostic properties of the GAmapTM Dysbiosis Test.

Results: The faecal microbiota in collagenous colitis resembles the faecalmicrobiota in inflammatory bowel disease. The faecal microbiota differs from the mucosal microbiota. Faecal microbiota at initiation of biological treatment among patients with Crohn’s disease differ between responders and non-responders. The GAmapTM Dysbiosis Test discriminates patients with inflammatory bowel disease from healthy individuals.

Conclusion: Collagenous colitis may share microbial underpinnings with other inflammatory bowel diseases. Conclusions about mucosal interactions with the gut microbiota should be made with caution when usingfaecal samples to characterise the microbiota. In Crohn’s disease, the faecal microbiota may be included in a model to predict the outcome of biological treatment. The GAmap Dysbiosis Test does not seem to be superior to other current diagnostic tools in clinical decision-making. 

Place, publisher, year, edition, pages
Örebro: Örebro University, 2021. p. 133
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 248
Keywords
Inflammatory bowel disease, microscopic colitis, Crohn’s disease, ulcerative colitis, microbiota, microbiome, biological treatment
National Category
General Practice
Identifiers
urn:nbn:se:oru:diva-93138 (URN)9789175294049 (ISBN)
Public defence
2021-10-14, Örebro universitet, Campus USÖ, hörsal C1, Södra Grev Rosengatan 32, Örebro, 09:00 (Swedish)
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Available from: 2021-07-26 Created: 2021-07-26 Last updated: 2021-10-22Bibliographically approved

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Carstens, AdamLindqvist, Carl MårtenBohr, JohanHalfvarson, Jonas

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