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DNA launched suicidal flaviviruses as therapeutic vaccine candidates
Örebro universitet, Institutionen för medicinska vetenskaper. (Inflammatory Response and Infection Susceptibility Centre (iRiSC))
School of Medical Sciences, Örebro University, Örebro, Sweden. (Inflammatory Response and Infection Susceptibility Centre (iRiSC))
Örebro universitet, Institutionen för medicinska vetenskaper. (Inflammatory Response and Infection Susceptibility Centre (iRiSC))
Örebro universitet, Institutionen för medicinska vetenskaper. (Inflammatory Response and Infection Susceptibility Centre (iRiSC))
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2018 (Engelska)Konferensbidrag, Poster (med eller utan abstract) (Refereegranskat)
Abstract [en]

Chronic liver disease, resulting from Hepatitis B virus (HBV), Hepatitis D virus (HDV), or Hepatitis C virus (HCV) infections, contributes to a major health burden worldwide. The relativelyhigh cost of the HCV treatment brings concerns about the accessibility, especially in the developing countries. Hence, there exists a need for cost effect interventions with high efficiency. We aim to develop therapeutic vaccine candidates against HBV, HCV and HDV using DNA based subgenomic flavivirus replicons as a delivery system. Tick-borne encephalitis virus (TBEV), Langat virus (LGTV), West-Nile virus (WNV), or Kunjinvirus (KUNV) replicon with firefly luciferase geneas a reporter were expressed and characterized in cell culture studies. WNV and KUNV replicons showed significantly higher replication compared to their respective negative controls with unfunctional viral RNA dependent RNA polymerase. KUNV and WNV replicons were chosen for cloning the HCV or HB/DV vaccine candidate gene by replacing luciferasegene. Owing to the self-replicating trait of the flavivirus subgenomic replicons, Western blotting demonstrated that the antigen expression by KUNV and WNV replicons was several folds higher than the positive control. These results suggest that DNA based KUNV and WNV replicons may function as carriers for the hepatitis vaccine candidate genes, and these replicons are currently used for in vivostudies in animal models.

Ort, förlag, år, upplaga, sidor
2018.
Nationell ämneskategori
Medicin och hälsovetenskap Infektionsmedicin Immunologi
Forskningsämne
Immunologi; Infektionssjukdomar
Identifikatorer
URN: urn:nbn:se:oru:diva-76632OAI: oai:DiVA.org:oru-76632DiVA, id: diva2:1353174
Konferens
15th Smögen Summer Symposium on Virology, Smögen, Sweden, August 23-25, 2018
Tillgänglig från: 2019-09-20 Skapad: 2019-09-20 Senast uppdaterad: 2019-09-23Bibliografiskt granskad

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Asghar, NaveedTran, Pham Tue HungMelik, WessamJohansson, Magnus

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Asghar, NaveedTran, Pham Tue HungMelik, WessamJohansson, Magnus
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Institutionen för medicinska vetenskaper
Medicin och hälsovetenskapInfektionsmedicinImmunologi

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