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Environmental chemicals differentially affect epigenetic-related mechanisms in the zebrafish liver (ZF-L) cell line and in zebrafish embryos
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre (MTM))
Institute for Environmental Medicine, Karolinska Institutet, Solna, Sweden.
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre (MTM); Örebro Life Science Centre)ORCID iD: 0000-0001-9713-2365
Örebro University, School of Science and Technology. (Man-Technology-Environment Research Centre (MTM))ORCID iD: 0000-0002-2356-6686
2019 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 215, article id 105272Article in journal (Refereed) Published
Abstract [en]

A number of chemicals have been shown to affect epigenetic patterning and functions. Since epigenetic mechanisms regulate transcriptional networks, epigenetic changes induced by chemical exposure can represent early molecular events for long-term adverse physiological effects. Epigenetics has thus appeared as a research field of major interest within (eco)toxicological sciences. The present study aimed at measuring effects on epigenetic-related mechanisms of selected environmental chemicals (bisphenols, perfluorinated chemicals, methoxychlor, permethrin, vinclozolin and coumarin 47) in zebrafish embryos and liver cells (ZFL). Transcription of genes related to DNA methylation and histone modifications was measured and global DNA methylation was assessed in ZFL cells using the LUMA assay. The differences in results gathered from both models suggest that chemicals affect different mechanisms related to epigenetics in embryos and cells. In zebrafish embryos, exposure to bisphenol A, coumarin 47, methoxychlor and permethrin lead to significant transcriptional changes in epigenetic factors suggesting that they can impact early epigenome reprogramming related to embryonic development. In ZFL cells, significant transcriptional changes were observed upon exposure to all chemicals but coumarin 47; however, only perfluorooctane sulfonate induced significant effects on global DNA methylation. Notably, in contrast to the other tested chemicals, perfluorooctane sulfonate affected only the expression of the histone demethylase kdm5ba. In addition, kdm5ba appeared as a sensitive gene in zebrafish embryos as well. Taken together, the present results suggest a role for kdm5ba in regulating epigenetic patterns in response to chemical exposure, even though mechanisms remain unclear. To confirm these findings, further evidence is required regarding changes in site-specific histone marks and DNA methylation together with their long-term effects on physiological outcomes.

Place, publisher, year, edition, pages
Elsevier, 2019. Vol. 215, article id 105272
Keywords [en]
Chemical pollutant, DNA methylation, Danio rerio, Histone modification, LUMA, qPCR
National Category
Developmental Biology
Identifiers
URN: urn:nbn:se:oru:diva-77033DOI: 10.1016/j.aquatox.2019.105272ISI: 000489354800005PubMedID: 31442592Scopus ID: 2-s2.0-85070872497OAI: oai:DiVA.org:oru-77033DiVA, id: diva2:1358071
Funder
Swedish Research Council Formas
Note

Funding Agency:

EnForce platform - KK Foundation  201660019

Available from: 2019-10-07 Created: 2019-10-07 Last updated: 2019-10-25Bibliographically approved

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Blanc, MélanieScherbak, NikolaiKeiter, Steffen

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