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Genetic variation regulates the activation and specificity of Restriction-Modification systems in Neisseria gonorrhoeae
Centre for Genomic Pathogen Surveillance, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK; Big Data Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
Örebro universitet, Institutionen för medicinska vetenskaper. WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, National Reference Laboratory for Sexually Transmitted Infections, Department of Laboratory Medicine, Clinical Microbiology.ORCID-id: 0000-0002-0688-2521
Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Örebro universitet, Institutionen för medicinska vetenskaper. Region Örebro län. WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, National Reference Laboratory for Sexually Transmitted Infections, Department of Laboratory Medicine, Clinical Microbiology.ORCID-id: 0000-0003-1710-2081
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2019 (Engelska)Ingår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, nr 1, artikel-id 14685Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Restriction-Modification systems (RMS) are one of the main mechanisms of defence against foreign DNA invasion and can have an important role in the regulation of gene expression. The obligate human pathogen Neisseria gonorrhoeae carries one of the highest loads of RMS in its genome; between 13 to 15 of the three main types. Previous work has described their organization in the reference genome FA1090 and has inferred the associated methylated motifs. Here, we studied the structure of RMS and target methylated motifs in 25 gonococcal strains sequenced with Single Molecule Real-Time (SMRT) technology, which provides data on DNA modification. The results showed a variable picture of active RMS in different strains, with phase variation switching the activity of Type III RMS, and both the activity and specificity of a Type I RMS. Interestingly, the Dam methylase was found in place of the NgoAXI endonuclease in two of the strains, despite being previously thought to be absent in the gonococcus. We also identified the real methylation target of NgoAXII as 5'-GCAGA-3', different from that previously described. Results from this work give further insights into the diversity and dynamics of RMS and methylation patterns in N. gonorrhoeae.

Ort, förlag, år, upplaga, sidor
Nature Publishing Group, 2019. Vol. 9, nr 1, artikel-id 14685
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Infektionsmedicin
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URN: urn:nbn:se:oru:diva-77256DOI: 10.1038/s41598-019-51102-2ISI: 000489701600021PubMedID: 31605008Scopus ID: 2-s2.0-85073164375OAI: oai:DiVA.org:oru-77256DiVA, id: diva2:1360613
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Funding Agency:

Wellcome Foundation for Medical Research at Örebro University Hospital, Örebro, Sweden 098051

Tillgänglig från: 2019-10-14 Skapad: 2019-10-14 Senast uppdaterad: 2019-10-25Bibliografiskt granskad

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Golparian, DanielUnemo, Magnus

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