oru.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
The photoreceptor UVR8 mediates the perception of both UV-B and UV-A wavelengths up to 350 nm of sunlight with responsivity moderated by cryptochromes
Helsingfors universitet.ORCID-id: 0000-0002-4972-9332
Örebro universitet, Institutionen för naturvetenskap och teknik. (Molekylär biokemi)ORCID-id: 0000-0002-8834-4064
Örebro universitet, Institutionen för naturvetenskap och teknik.
Helsingfors universitet.
Visa övriga samt affilieringar
(Engelska)Manuskript (preprint) (Övrigt vetenskapligt)
Abstract [en]

The photoreceptors UV RESISTANCE LOCUS 8 (UVR8) and CRYPTOCHROMES 1 and 2 (CRYs) play major roles in the perception of UV-B (280–315 nm) and UV-A/blue radiation (315–500 nm), respectively. However, it is poorly understood how they function in sunlight. The roles of UVR8 and CRYs were assessed in a factorial experiment with Arabidopsis thaliana wild-type and photoreceptor mutants exposed to sunlight for 6 h or 12 h under five types of filters with cut-offs in UV and blue-light regions. Transcriptome-wide responses triggered by UV-B and UV-A wavelengths shorter than 350 nm (UV-Asw) required UVR8 whereas those induced by blue and UV-A wavelengths longer than 350 nm (UV-Alw) required CRYs. UVR8 modulated gene expression in response to blue light while lack of CRYs drastically enhanced gene expression in response to UV-B and UV-Asw. These results agree with our estimates of photons absorbed by these photoreceptors in sunlight and with in vitro monomerization of UVR8 by wavelengths up to 335 nm. Motif enrichment analysis predicted complex signaling downstream of UVR8 and CRYs. Our results highlight that it is important to use UV waveband definitions specific to plants’ photomorphogenesis as is routinely done in the visible region.

Nyckelord [en]
Arabidopsis thaliana, blue light, cryptochrome, gene expression, photoreceptor interaction, solar radiation, ultraviolet radiation, UVR8
Nationell ämneskategori
Botanik Biokemi och molekylärbiologi
Forskningsämne
Biokemi
Identifikatorer
URN: urn:nbn:se:oru:diva-79340DOI: 10.1101/2020.01.21.913814.OAI: oai:DiVA.org:oru-79340DiVA, id: diva2:1387909
Forskningsfinansiär
Forskningsrådet Formas, 942-2015-516KK-stiftelsen, 20130164Finlands Akademi, 252548Finlands Akademi, 307335Tillgänglig från: 2020-01-22 Skapad: 2020-01-22 Senast uppdaterad: 2020-01-22

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextBioRxiv

Sök vidare i DiVA

Av författaren/redaktören
Rai, NehaO'Hara, AndrewJenkins, Gareth IStrid, ÅkeAphalo, Pedro JMorales, Luis Orlando
Av organisationen
Institutionen för naturvetenskap och teknik
BotanikBiokemi och molekylärbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 8 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf