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A combination of the activation marker CD86 and the immune checkpoint marker B and T lymphocyte attenuator (BTLA) indicates a putative permissive activation state of B cell subtypes in healthy blood donors independent of age and sex
Department of Pathology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Clinical Epidemiology and Biostatistics, School of Medical Sciences, Örebro University, Örebro, Sweden.
Örebro University, School of Medical Sciences. Department of Infectious Diseases.ORCID iD: 0000-0001-7679-7253
Department of Clinical Immunology and Transfusion Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
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2020 (English)In: BMC Immunology, ISSN 1471-2172, E-ISSN 1471-2172, Vol. 21, no 1, article id 14Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: The use of anti-B cell based therapies in immune-mediated diseases targeting general B cell markers or molecules important for B cell function has increased the clinical needs of monitoring B cell subpopulations.

RESULTS: We analyzed the expression profile of cell surface markers CD86 and B and T lymphocyte attenuator (BTLA) in B cell subtypes using flow cytometry, including naïve, transitional, switched memory, non-switched memory and double-negative memory B cells and plasmablasts, and investigated the dependence of age and sex in a healthy adult blood donor population. The switched memory B cell subtype displayed a divergent expression of the markers, with increased CD86 and decreased BTLA as compared to non-switched and double negative memory cells, as well as compared to naïve B cells. Plasmablasts expressed highly increased CD86 compared to all other subtypes and a decreased expression of BTLA compared to naïve cells, but still higher compared to the memory cell populations. Transitional B cells had CD86 and BTLA expression similar to the other naïve cells.

CONCLUSIONS: We show divergent expression of CD86 and BTLA in memory cells and plasmablasts compared to naïve B cells independent of age and sex. Furthermore, a similarly divergent difference of expression pattern was seen between the memory cell subtypes, altogether indicating that the combination of CD86 and BTLA might be markers for a permissive activation state. We suggest the combination of CD86 and BTLA expression on B cell subtypes as a potentially important tool in monitoring the status of B cell subtypes before and after treatments influencing the B cell compartment.

Place, publisher, year, edition, pages
BioMed Central, 2020. Vol. 21, no 1, article id 14
Keywords [en]
B cell, B cell subtype, BTLA, CD86
National Category
Immunology
Identifiers
URN: urn:nbn:se:oru:diva-80802DOI: 10.1186/s12865-020-00343-2ISI: 000521993300001PubMedID: 32197584Scopus ID: 2-s2.0-85082146506OAI: oai:DiVA.org:oru-80802DiVA, id: diva2:1416426
Note

Funding Agencies:

ALF  

Örebro University 

Available from: 2020-03-23 Created: 2020-03-23 Last updated: 2020-04-07Bibliographically approved

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Lange, AnnaHultgren Hörnquist, ElisabethHultgren, Olof

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