Optimal control of inlet jet flows is of wide applicative interest in order to enhance mixing and reduce stagnation in a ventilated room. The general approach in mechanical ventilation is to use a constant flow rate forced convection system providing the ventilation air. This type of ventilation may cause several problems such as draught, stagnation at certain occupied locations, and subsequently low ventilation efficiencies. An alternative to increase the ventilation quality that has been of interest in this study is to introduce flow variations, which is considered as a potential to reduce stagnation and increase efficiency of the ventilation. The study was conducted as a model experiment in a small-scale, two-dimensional (2-D) room model with dimensions 30200.9 cm3 with water as operating fluid. The size of the model made it possible to investigate the 2-D velocity vector field within the entire room using Particle Image Velocimetry (PIV) method and further consequent dynamical and statistical analyses have been done from the resulted PIV vector fields. The comparison between cases of constant flow rate and flow variations have been conducted for the cases of two set of base flow rates and for each one, the cases of constant flow rate and flow variations with frequencies of 0.3, 0.4 and 0.5 Hz, is considered. In this investigation we show that the calm region, with a large stagnation zone, without pulsating inflow condition becomes more active in the sense that the stagnation points are moved and that the small-scale structures are grown for increasing pulsation frequency.